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The map label for this gene is dagK [H]

Identifier: 162329651

GI number: 162329651

Start: 436267

End: 437298

Strand: Direct

Name: dagK [H]

Synonym: llmg_0445

Alternate gene names: 162329651

Gene position: 436267-437298 (Clockwise)

Preceding gene: 125623311

Following gene: 125623313

Centisome position: 17.25

GC content: 36.63

Gene sequence:

>1032_bases
ATGATGAAGGCGAGATTGATTTACAATCCGACATCGGGTCAAGAAATTATAAAAAAACATATTGCTGATATTTTGGATAA
ATTAGAGCAATATGGGTATGAAGCAAGTGCTTATCAAACGACAGCAGAACAAGATTCGGCTAAGAAAGAAGCAGCACGAG
CAACGCAAGCTGGATTTGATTTAATCATTGCAGCAGGTGGAGATGGCACAATTAATGAAGTTGTGGCCGGAATTTCTCCC
TTTGAGAAACGACCACGATTGGCCATTGTCCCAACGGGAACAACTAATGATTTTGCAAGAGCACTGAAGATTCCACGAGG
AAAACCACTGGAAGCAATCGAAATCATTGGTAAAAATCAAATTTTAAATATTGACGTTGGTCATGCCATTATTAAAGAAA
CCAAAGATGAGCAATATTTTATTAATATTGCTGCCGGTGGAGGGCTGACAGAACTTACTTACAGTGTTCCAAGCCATTTA
AAAACGGCATTTGGTTATTTGGCTTATTTAGCCAAAGGTGCAGAACTTTTACCTCGTGTCCGCAAAGCACCGGTTCGTGT
TGTTCATGATGAGGGTGTCTTTGAAGGTGATATTTCAATGTTTTTTGCGGCACTGACTAATTCAGTTGGTGGTTTTGAAA
AGATTGCGCCTGATGCAAAGTTAGATGATGGACTGTTCACACTTATTTTAGTAAAAACTGATAATTTATTTGAATTGATT
GCTTTACTTGCAATTGTAGCCAATGGAAAACATTTGGATGATGTTAATTTGGAGTATATAAAAACAAGTAAAATTGAGAT
AGAAGCTTTAGGTGGACAATCAATCCTTCTTAATTTGGATGGTGAATATGGCGGAGATGCTCCTGTTCAATTTGATAATT
TAAAAGGGCATTTGGATATGTATGTCAATCTTGATGAAATCAATGATGACCATTATATTGGCGATGAAGAAACTTTAGCT
TTGGAAGCAGTTGCTCAAAAATTTGCTGAAGAAGCAAATAAGATTAAAGATGAAGATGAAACAGATCAATAA

Upstream 100 bases:

>100_bases
AAGAGATTTACTGACAGAATTGTAAAAGCCTAAGGGCTACTCTCTGACAAAGCCGTCAGTAAATAATTTAAAAAGAATGG
ATGAGAAGTTTTCGGGGGAA

Downstream 100 bases:

>100_bases
ATGATAAAAGTTGCTGACAGAGTTGCCAGTGACTTTTTTTGATTCTGTCAGCAAAAAGAAAAGATAATTTTAAAATTGTG
AATAAGCGTGTGGTTTAATT

Product: putative lipid kinase

Products: NA

Alternate protein names: DAG kinase; DAGK [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MMKARLIYNPTSGQEIIKKHIADILDKLEQYGYEASAYQTTAEQDSAKKEAARATQAGFDLIIAAGGDGTINEVVAGISP
FEKRPRLAIVPTGTTNDFARALKIPRGKPLEAIEIIGKNQILNIDVGHAIIKETKDEQYFINIAAGGGLTELTYSVPSHL
KTAFGYLAYLAKGAELLPRVRKAPVRVVHDEGVFEGDISMFFAALTNSVGGFEKIAPDAKLDDGLFTLILVKTDNLFELI
ALLAIVANGKHLDDVNLEYIKTSKIEIEALGGQSILLNLDGEYGGDAPVQFDNLKGHLDMYVNLDEINDDHYIGDEETLA
LEAVAQKFAEEANKIKDEDETDQ

Sequences:

>Translated_343_residues
MMKARLIYNPTSGQEIIKKHIADILDKLEQYGYEASAYQTTAEQDSAKKEAARATQAGFDLIIAAGGDGTINEVVAGISP
FEKRPRLAIVPTGTTNDFARALKIPRGKPLEAIEIIGKNQILNIDVGHAIIKETKDEQYFINIAAGGGLTELTYSVPSHL
KTAFGYLAYLAKGAELLPRVRKAPVRVVHDEGVFEGDISMFFAALTNSVGGFEKIAPDAKLDDGLFTLILVKTDNLFELI
ALLAIVANGKHLDDVNLEYIKTSKIEIEALGGQSILLNLDGEYGGDAPVQFDNLKGHLDMYVNLDEINDDHYIGDEETLA
LEAVAQKFAEEANKIKDEDETDQ
>Mature_343_residues
MMKARLIYNPTSGQEIIKKHIADILDKLEQYGYEASAYQTTAEQDSAKKEAARATQAGFDLIIAAGGDGTINEVVAGISP
FEKRPRLAIVPTGTTNDFARALKIPRGKPLEAIEIIGKNQILNIDVGHAIIKETKDEQYFINIAAGGGLTELTYSVPSHL
KTAFGYLAYLAKGAELLPRVRKAPVRVVHDEGVFEGDISMFFAALTNSVGGFEKIAPDAKLDDGLFTLILVKTDNLFELI
ALLAIVANGKHLDDVNLEYIKTSKIEIEALGGQSILLNLDGEYGGDAPVQFDNLKGHLDMYVNLDEINDDHYIGDEETLA
LEAVAQKFAEEANKIKDEDETDQ

Specific function: Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is a key enzyme involved in the production of lipoteichoic acid by reintroducing DAG formed from the breakdown of membrane phospholipids into the phosphatidylglycerol biosynthet

COG id: COG1597

COG function: function code IR; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DAGKc domain [H]

Homologues:

Organism=Escherichia coli, GI1788402, Length=249, Percent_Identity=30.5220883534137, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017438
- InterPro:   IPR005218
- InterPro:   IPR001206 [H]

Pfam domain/function: PF00781 DAGK_cat [H]

EC number: =2.7.1.107 [H]

Molecular weight: Translated: 37410; Mature: 37410

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKARLIYNPTSGQEIIKKHIADILDKLEQYGYEASAYQTTAEQDSAKKEAARATQAGFD
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHCHHHHHHHHHHHHHHHHCCCE
LIIAAGGDGTINEVVAGISPFEKRPRLAIVPTGTTNDFARALKIPRGKPLEAIEIIGKNQ
EEEEECCCCCHHHHHHCCCCHHHCCCEEEEECCCCHHHHHHHCCCCCCCCHHHEEECCCE
ILNIDVGHAIIKETKDEQYFINIAAGGGLTELTYSVPSHLKTAFGYLAYLAKGAELLPRV
EEEEECCHHHHHCCCCCEEEEEEECCCCHHEEEEHHHHHHHHHHHHHHHHHCCHHHHHHH
RKAPVRVVHDEGVFEGDISMFFAALTNSVGGFEKIAPDAKLDDGLFTLILVKTDNLFELI
HCCCEEEEECCCCEEHHHHHHHHHHHHCCCCHHHHCCCCCCCCCEEEEEEEECCCHHHHH
ALLAIVANGKHLDDVNLEYIKTSKIEIEALGGQSILLNLDGEYGGDAPVQFDNLKGHLDM
HHHHHHHCCCCCCCCCEEEEEECEEEEEEECCCEEEEEECCCCCCCCCEEECCCCCEEEE
YVNLDEINDDHYIGDEETLALEAVAQKFAEEANKIKDEDETDQ
EEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MMKARLIYNPTSGQEIIKKHIADILDKLEQYGYEASAYQTTAEQDSAKKEAARATQAGFD
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHCHHHHHHHHHHHHHHHHCCCE
LIIAAGGDGTINEVVAGISPFEKRPRLAIVPTGTTNDFARALKIPRGKPLEAIEIIGKNQ
EEEEECCCCCHHHHHHCCCCHHHCCCEEEEECCCCHHHHHHHCCCCCCCCHHHEEECCCE
ILNIDVGHAIIKETKDEQYFINIAAGGGLTELTYSVPSHLKTAFGYLAYLAKGAELLPRV
EEEEECCHHHHHCCCCCEEEEEEECCCCHHEEEEHHHHHHHHHHHHHHHHHCCHHHHHHH
RKAPVRVVHDEGVFEGDISMFFAALTNSVGGFEKIAPDAKLDDGLFTLILVKTDNLFELI
HCCCEEEEECCCCEEHHHHHHHHHHHHCCCCHHHHCCCCCCCCCEEEEEEEECCCHHHHH
ALLAIVANGKHLDDVNLEYIKTSKIEIEALGGQSILLNLDGEYGGDAPVQFDNLKGHLDM
HHHHHHHCCCCCCCCCEEEEEECEEEEEEECCCEEEEEECCCCCCCCCEEECCCCCEEEE
YVNLDEINDDHYIGDEETLALEAVAQKFAEEANKIKDEDETDQ
EEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]