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The map label for this gene is trmE [H]

Identifier: 125625274

GI number: 125625274

Start: 2477220

End: 2478587

Strand: Direct

Name: trmE [H]

Synonym: llmg_2521

Alternate gene names: 125625274

Gene position: 2477220-2478587 (Clockwise)

Preceding gene: 125625271

Following gene: 125625280

Centisome position: 97.93

GC content: 38.96

Gene sequence:

>1368_bases
ATGACATTAACACAAGAATTTGATACGATTGCGGCGATTTCGACACCGCTTGGTGAAGGAGCAATTGCCATTGTTCGTTT
ATCAGGAACAGATGCCCTGAAAATTGCTCAATCTGTCTATAAAGGAAAAAATTTAGCTCAAGTTGCTAGCCATACGATTA
ATTACGGCCACATCTTTGAAGAAGAACGTCTGGTTGACGAAGTGATGGTTTCAGTCATGCGTGCGCCTAAAACTTTCACT
CGTGAAGATATTGTTGAAATCAACACACACGGTGGGATTGCAGTCACACAAGAAATCTTGCAATTACTTTTACGTAATGG
TGCAAGACTTGCTGAACCTGGTGAATTTACCAAGCGTGCTTTCTTAAATGGACGGATTGACTTGGCACAAGCTGAGTCTG
TCATGGATTTAATCCGCGCAAAAACCGATAAAGCAGCGAATATTGCCGTAAAACAACTTGACGGAAGTCTCTCAAAAATG
ATTAATAATATTCGTCAAGAGATTCTCGAAAGTTTAGCACAAGTCGAGGTCAATATTGATTATCCAGAGTATGATGATGT
CGAAACCATGACTTCACAAATGCTCCTTGAAAAGACTGCTCATTTTGAACAGCTTTTGGAAAATCTTTTATCAACAGCTA
AACGCGGAAAAATTTTGCGCGAAGGCTTAAAAACGGCCATTATTGGACGTCCAAACGTTGGAAAATCAAGCTTACTCAAT
CAATTATTGCGTGAAGAAAAAGCCATTGTCACTGACATTGCTGGAACAACACGTGATGTTATTACCGAGTTTGCCAATAT
TGGTGGCGTTCCTCTTGAATTAGTAGACACCGCTGGGATTCGTGAAACAGATGATTTAGTCGAAGCGATTGGGATTGAAC
GTTCTAAAAAGGCCTTAGCCGAAGCTGATTTGGTTTTGCTAGTTTTGGACGCCTCACTGGAATTGACTGATAAAGATTTG
GAACTTTTAGAGCTATCTAAAAATGCCAATCGTATTGTTTTACTTAACAAAACTGATTTACCCGAAAAACTTGATATTAA
TCAAATTTCCGGTGATTTTATTCGAATTTCAGCCCTTAAAAATGAGAATTTATCTGCTGTTGAAGAAAAAATCAATCAAA
TTTTCTTTGCTGGTGAAATTGAAGCCAAAGACGCAACCGTCTTATCAAATGCTCGTCACATTTCGCTTGTTGAAGAAGCT
TTAAAAGCACTCAAGGAAGCAAATAACGGGCTTGCTCTTGGCTTACCTGTTGATTTGATTCAAGTCGATGTCACACGTTG
TTGGCAATTACTTGGAGAAATCACAGGCGAAGCCGCTCCTGATGAATTGATTACACAACTTTTCTCACAATTTTGTTTAG
GGAAATAA

Upstream 100 bases:

>100_bases
TTTATGTGTGGAAACTCAATCTTAATAGAATTTTATTTTTTATAAAAAAATAAATTAAATAGGCTAGGAAATTCAAGCTT
TTTGCGTTAAAATAGTCTTT

Downstream 100 bases:

>100_bases
AAAAGAGTTCAATTGAACTCTTTTTTTACTGACAGAATTGATCAATCATCAAACCATCCCTTTAATTTCTGTCAGACATT
TTCTAACAAACTGGTCAGTC

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MTLTQEFDTIAAISTPLGEGAIAIVRLSGTDALKIAQSVYKGKNLAQVASHTINYGHIFEEERLVDEVMVSVMRAPKTFT
REDIVEINTHGGIAVTQEILQLLLRNGARLAEPGEFTKRAFLNGRIDLAQAESVMDLIRAKTDKAANIAVKQLDGSLSKM
INNIRQEILESLAQVEVNIDYPEYDDVETMTSQMLLEKTAHFEQLLENLLSTAKRGKILREGLKTAIIGRPNVGKSSLLN
QLLREEKAIVTDIAGTTRDVITEFANIGGVPLELVDTAGIRETDDLVEAIGIERSKKALAEADLVLLVLDASLELTDKDL
ELLELSKNANRIVLLNKTDLPEKLDINQISGDFIRISALKNENLSAVEEKINQIFFAGEIEAKDATVLSNARHISLVEEA
LKALKEANNGLALGLPVDLIQVDVTRCWQLLGEITGEAAPDELITQLFSQFCLGK

Sequences:

>Translated_455_residues
MTLTQEFDTIAAISTPLGEGAIAIVRLSGTDALKIAQSVYKGKNLAQVASHTINYGHIFEEERLVDEVMVSVMRAPKTFT
REDIVEINTHGGIAVTQEILQLLLRNGARLAEPGEFTKRAFLNGRIDLAQAESVMDLIRAKTDKAANIAVKQLDGSLSKM
INNIRQEILESLAQVEVNIDYPEYDDVETMTSQMLLEKTAHFEQLLENLLSTAKRGKILREGLKTAIIGRPNVGKSSLLN
QLLREEKAIVTDIAGTTRDVITEFANIGGVPLELVDTAGIRETDDLVEAIGIERSKKALAEADLVLLVLDASLELTDKDL
ELLELSKNANRIVLLNKTDLPEKLDINQISGDFIRISALKNENLSAVEEKINQIFFAGEIEAKDATVLSNARHISLVEEA
LKALKEANNGLALGLPVDLIQVDVTRCWQLLGEITGEAAPDELITQLFSQFCLGK
>Mature_454_residues
TLTQEFDTIAAISTPLGEGAIAIVRLSGTDALKIAQSVYKGKNLAQVASHTINYGHIFEEERLVDEVMVSVMRAPKTFTR
EDIVEINTHGGIAVTQEILQLLLRNGARLAEPGEFTKRAFLNGRIDLAQAESVMDLIRAKTDKAANIAVKQLDGSLSKMI
NNIRQEILESLAQVEVNIDYPEYDDVETMTSQMLLEKTAHFEQLLENLLSTAKRGKILREGLKTAIIGRPNVGKSSLLNQ
LLREEKAIVTDIAGTTRDVITEFANIGGVPLELVDTAGIRETDDLVEAIGIERSKKALAEADLVLLVLDASLELTDKDLE
LLELSKNANRIVLLNKTDLPEKLDINQISGDFIRISALKNENLSAVEEKINQIFFAGEIEAKDATVLSNARHISLVEEAL
KALKEANNGLALGLPVDLIQVDVTRCWQLLGEITGEAAPDELITQLFSQFCLGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=464, Percent_Identity=32.9741379310345, Blast_Score=203, Evalue=3e-52,
Organism=Homo sapiens, GI193082988, Length=495, Percent_Identity=30.9090909090909, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI193082993, Length=454, Percent_Identity=31.9383259911894, Blast_Score=187, Evalue=2e-47,
Organism=Escherichia coli, GI2367268, Length=457, Percent_Identity=35.4485776805252, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI87082120, Length=170, Percent_Identity=36.4705882352941, Blast_Score=93, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17507259, Length=461, Percent_Identity=30.58568329718, Blast_Score=184, Evalue=8e-47,
Organism=Saccharomyces cerevisiae, GI6323665, Length=490, Percent_Identity=29.3877551020408, Blast_Score=193, Evalue=5e-50,
Organism=Drosophila melanogaster, GI45550813, Length=478, Percent_Identity=30.5439330543933, Blast_Score=203, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR003661
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 49885; Mature: 49754

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLTQEFDTIAAISTPLGEGAIAIVRLSGTDALKIAQSVYKGKNLAQVASHTINYGHIFE
CCCCHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHCCCHHHHH
EERLVDEVMVSVMRAPKTFTREDIVEINTHGGIAVTQEILQLLLRNGARLAEPGEFTKRA
HHHHHHHHHHHHHHCCCCCCHHCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHH
FLNGRIDLAQAESVMDLIRAKTDKAANIAVKQLDGSLSKMINNIRQEILESLAQVEVNID
HHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHEEEECC
YPEYDDVETMTSQMLLEKTAHFEQLLENLLSTAKRGKILREGLKTAIIGRPNVGKSSLLN
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHH
QLLREEKAIVTDIAGTTRDVITEFANIGGVPLELVDTAGIRETDDLVEAIGIERSKKALA
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEHHCCCCCCCHHHHHHHHCCCHHHHHHH
EADLVLLVLDASLELTDKDLELLELSKNANRIVLLNKTDLPEKLDINQISGDFIRISALK
HHCEEEEEECCCCCCCCCCHHHHHHCCCCCEEEEEECCCCCCCCCCHHCCCCEEEEEEEC
NENLSAVEEKINQIFFAGEIEAKDATVLSNARHISLVEEALKALKEANNGLALGLPVDLI
CCCHHHHHHHHHHEEEECCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCEEECCCHHHH
QVDVTRCWQLLGEITGEAAPDELITQLFSQFCLGK
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLTQEFDTIAAISTPLGEGAIAIVRLSGTDALKIAQSVYKGKNLAQVASHTINYGHIFE
CCCHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHCCCHHHHH
EERLVDEVMVSVMRAPKTFTREDIVEINTHGGIAVTQEILQLLLRNGARLAEPGEFTKRA
HHHHHHHHHHHHHHCCCCCCHHCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHH
FLNGRIDLAQAESVMDLIRAKTDKAANIAVKQLDGSLSKMINNIRQEILESLAQVEVNID
HHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHEEEECC
YPEYDDVETMTSQMLLEKTAHFEQLLENLLSTAKRGKILREGLKTAIIGRPNVGKSSLLN
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHH
QLLREEKAIVTDIAGTTRDVITEFANIGGVPLELVDTAGIRETDDLVEAIGIERSKKALA
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEHHCCCCCCCHHHHHHHHCCCHHHHHHH
EADLVLLVLDASLELTDKDLELLELSKNANRIVLLNKTDLPEKLDINQISGDFIRISALK
HHCEEEEEECCCCCCCCCCHHHHHHCCCCCEEEEEECCCCCCCCCCHHCCCCEEEEEEEC
NENLSAVEEKINQIFFAGEIEAKDATVLSNARHISLVEEALKALKEANNGLALGLPVDLI
CCCHHHHHHHHHHEEEECCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCEEECCCHHHH
QVDVTRCWQLLGEITGEAAPDELITQLFSQFCLGK
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]