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The map label for this gene is ytzG [H]

Identifier: 125625272

GI number: 125625272

Start: 2475290

End: 2476276

Strand: Reverse

Name: ytzG [H]

Synonym: llmg_2518

Alternate gene names: 125625272

Gene position: 2476276-2475290 (Counterclockwise)

Preceding gene: 125625273

Following gene: 125625270

Centisome position: 97.9

GC content: 36.68

Gene sequence:

>987_bases
CTGACCAGATTTCTGTCAGTAACTTTTATTTTTACTGACAGAATTTTCGCCAATAAAATAAGGAGTATCACTACCTGTAA
TAGGCTAAAGTGTGAATTCGCAGACAAAAGTTGCTCAAGTGATTTTTTAGATACCTCATTAGTCACTTCTGGTGAAAATA
CTAGCATATTCGTAAGTCAGCAAGCTGACAAGGACTACCCTAAGTGGGAAATTATTGTTCCCGCTTCGTTCGGTTATTAT
CTATGCTATAATAAAGGCATGAGACTAGATAAATTTTTAGCAGAAAGTGGCCTCGGAAGCCGGAGTGAAGTCAAAGTTTT
ATTGAAAAAAGGGCGCGTGACCGTTGCTGGTGAAATTCAGCGAGATGGCAGATTTCAAGTGGATAAGAATTCCAGACAGG
TGGCTTTTGACGGTCAAATTTTAGATTACCAAGAATATTATTACTACCTATTGAATAAACCGCAAGGAGTCGTATCCGCT
ACGAAAGATAATGTTTTTAAAACAGTTGTTCAACTTTTATCAGAAAAAGATTTTCGTAAGGATATTTTCCCAGTCGGACG
TTTAGATAAAAATACCGAAGGACTTTTGATTTTAACCAATGATGGGGCTCTTGGCCATGATTTAACTCAACCCAAAAAAC
ACGTTGAAAAAGAATATTTTGCACGAATTGATGGAAAAGTTACTGACGAGATTATCAATAAATTTGCTGAAGGTTTGACT
TTGAAAAATGGTGAAAAAGCAAGACCAGGAGAATTATTTGTTGAGAAAACTTACAAAGGCGATAGCCAATCCGGCTGGGT
CAGTGAAATTCGAGTTATTATTCATGAAGGAAAATTCCACCAAGTCAAACGGATGTTTGAGGCAGTTGAGATGTATGTCC
TTTATCTCAAACGAATTCGGATGGGCAATTTAGCTTTGGATTCAGATTTAGCTCTGGGAGCTTATCGTCCTTTAACAATC
GAAGAGATTGAAAACTTAAAAAAATAA

Upstream 100 bases:

>100_bases
ATGATGACAGTTTTGCAAATCAATTGGCAGCTTATCCACCTGAAACAATGGTTATTTTTAAACTTACAGATGCTGATATT
TATGGAGAATAAAAAAGTTA

Downstream 100 bases:

>100_bases
AAGTTACTGATGAAAATTCGAAGGTATCCGTAAGCTAAAGTGCAACGGCTATCCTCTGACAGAATTTTTCGTCAGTAACT
TTTTTAATTTTCACGATTAA

Product: ribosomal small subunit pseudouridine synthase A

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MTRFLSVTFIFTDRIFANKIRSITTCNRLKCEFADKSCSSDFLDTSLVTSGENTSIFVSQQADKDYPKWEIIVPASFGYY
LCYNKGMRLDKFLAESGLGSRSEVKVLLKKGRVTVAGEIQRDGRFQVDKNSRQVAFDGQILDYQEYYYYLLNKPQGVVSA
TKDNVFKTVVQLLSEKDFRKDIFPVGRLDKNTEGLLILTNDGALGHDLTQPKKHVEKEYFARIDGKVTDEIINKFAEGLT
LKNGEKARPGELFVEKTYKGDSQSGWVSEIRVIIHEGKFHQVKRMFEAVEMYVLYLKRIRMGNLALDSDLALGAYRPLTI
EEIENLKK

Sequences:

>Translated_328_residues
MTRFLSVTFIFTDRIFANKIRSITTCNRLKCEFADKSCSSDFLDTSLVTSGENTSIFVSQQADKDYPKWEIIVPASFGYY
LCYNKGMRLDKFLAESGLGSRSEVKVLLKKGRVTVAGEIQRDGRFQVDKNSRQVAFDGQILDYQEYYYYLLNKPQGVVSA
TKDNVFKTVVQLLSEKDFRKDIFPVGRLDKNTEGLLILTNDGALGHDLTQPKKHVEKEYFARIDGKVTDEIINKFAEGLT
LKNGEKARPGELFVEKTYKGDSQSGWVSEIRVIIHEGKFHQVKRMFEAVEMYVLYLKRIRMGNLALDSDLALGAYRPLTI
EEIENLKK
>Mature_327_residues
TRFLSVTFIFTDRIFANKIRSITTCNRLKCEFADKSCSSDFLDTSLVTSGENTSIFVSQQADKDYPKWEIIVPASFGYYL
CYNKGMRLDKFLAESGLGSRSEVKVLLKKGRVTVAGEIQRDGRFQVDKNSRQVAFDGQILDYQEYYYYLLNKPQGVVSAT
KDNVFKTVVQLLSEKDFRKDIFPVGRLDKNTEGLLILTNDGALGHDLTQPKKHVEKEYFARIDGKVTDEIINKFAEGLTL
KNGEKARPGELFVEKTYKGDSQSGWVSEIRVIIHEGKFHQVKRMFEAVEMYVLYLKRIRMGNLALDSDLALGAYRPLTIE
EIENLKK

Specific function: Responsible For Synthesis Of Pseudouridine From Uracil-516 In 16s Ribosomal RNA. [C]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=242, Percent_Identity=40.495867768595, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1787524, Length=252, Percent_Identity=30.5555555555556, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1790453, Length=249, Percent_Identity=30.5220883534137, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI87081838, Length=178, Percent_Identity=32.5842696629214, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 37506; Mature: 37375

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRFLSVTFIFTDRIFANKIRSITTCNRLKCEFADKSCSSDFLDTSLVTSGENTSIFVSQ
CCCEEEEEEEEEHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHEECCCCCEEEEEE
QADKDYPKWEIIVPASFGYYLCYNKGMRLDKFLAESGLGSRSEVKVLLKKGRVTVAGEIQ
CCCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEEC
RDGRFQVDKNSRQVAFDGQILDYQEYYYYLLNKPQGVVSATKDNVFKTVVQLLSEKDFRK
CCCCEEECCCCCEEEECCEEECHHHEEEEEECCCCCCEECHHHHHHHHHHHHHCCCHHHH
DIFPVGRLDKNTEGLLILTNDGALGHDLTQPKKHVEKEYFARIDGKVTDEIINKFAEGLT
HCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCE
LKNGEKARPGELFVEKTYKGDSQSGWVSEIRVIIHEGKFHQVKRMFEAVEMYVLYLKRIR
ECCCCCCCCCCEEEEEECCCCCCCCHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
MGNLALDSDLALGAYRPLTIEEIENLKK
CCCEEECCCCCCCCCCCCCHHHHHHCCC
>Mature Secondary Structure 
TRFLSVTFIFTDRIFANKIRSITTCNRLKCEFADKSCSSDFLDTSLVTSGENTSIFVSQ
CCEEEEEEEEEHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHEECCCCCEEEEEE
QADKDYPKWEIIVPASFGYYLCYNKGMRLDKFLAESGLGSRSEVKVLLKKGRVTVAGEIQ
CCCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEEC
RDGRFQVDKNSRQVAFDGQILDYQEYYYYLLNKPQGVVSATKDNVFKTVVQLLSEKDFRK
CCCCEEECCCCCEEEECCEEECHHHEEEEEECCCCCCEECHHHHHHHHHHHHHCCCHHHH
DIFPVGRLDKNTEGLLILTNDGALGHDLTQPKKHVEKEYFARIDGKVTDEIINKFAEGLT
HCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCE
LKNGEKARPGELFVEKTYKGDSQSGWVSEIRVIIHEGKFHQVKRMFEAVEMYVLYLKRIR
ECCCCCCCCCCEEEEEECCCCCCCCHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
MGNLALDSDLALGAYRPLTIEEIENLKK
CCCEEECCCCCCCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]