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The map label for this gene is polA [H]

Identifier: 125625183

GI number: 125625183

Start: 2367757

End: 2370390

Strand: Reverse

Name: polA [H]

Synonym: llmg_2425

Alternate gene names: 125625183

Gene position: 2370390-2367757 (Counterclockwise)

Preceding gene: 125625185

Following gene: 125625182

Centisome position: 93.71

GC content: 37.62

Gene sequence:

>2634_bases
ATGGAAGATAAGAATAGACTTTTGCTAATTGATGGGTCATCGGTTGCTTTTCGAGCGTTTTTCGCATTATATAATCAGTT
AGATCGGTTCAAAGCACCTAATGGCTTACATACAAATGCCATTTTTGCTTTTCATACGATGTTATCTTCTTTGATGGAAC
GAATTCAACCGACACATGTGTTGATTGCTTTTGATGCGGGGAAAACCACTTTTCGGACGGAAATGTTTGCGGATTATAAA
GGGGGACGGTCAAAAACACCCGATGAGTTTCGCGAGCAATTGCCTTTTATTAAGGAAATGATTGAGAAGTTAGGAATTCG
ACACTATGAGTTGGCAAACTATGAGGCTGATGATATTATCGGAACTTTGGATAAAATGGCTGAAGCGCCTGATGTGAATT
TCGATGTGACCATTGTGACCGGGGACAAGGATATGATTCAGCTGGTCGATGGAAATACACGAGTGGAAATTTCTAAAAAG
GGTGTGGCTGAGTTTGAGGAATTTACACCTGACTATCTCTTAGAAAAAATGGGACTTACGCCATCACAATTTATTGATTT
GAAGGCTTTGATGGGCGATTCTTCGGATAATTATCCTGGCGTCACTAAAGTTGGTGAAAAAACGGGTCTTAAACTTTTAC
AGGAATTTGGTTCACTGGAAAATCTTTATGAAAATGTTGAGACGCTAAAAGCCAGCAAGATGAAGGATAATCTAATTGCT
GACAAGGAAATGGCCTTTTTGTCACAGCAATTGGCGACAATCAATACGAAAGCTCCTCTTGAAATTGGTTTAGAAGATAC
GCTTTTAAAAGAAAAAAATGTGGCTGAACTGGGTCAATTTTATGATGAAATGGGATTTTCACAATTCAAAAGTAAGTTAT
TAGCTGACGGCGGTGGAGAAGTTACTGACGAAACTGTCAGTGAAGAAATTAAGTTTGAAATAGTTACTGACAAAAGTTCA
GTTGCTTCAGTCAATGCTGATGATTTCTTTTATTTGGAAACTTTGGGTGAAAATTATCATCGTGAGCAGATTGTGGCATT
TGCTTGGGGAAATTCTGAGAAAATTTATGTTTCAAAAAATCTAGATTTACTGACAGAAATGAAATTTCCTGAAAATACTT
ATGATTTCAAGAAAAATCGCGTACTTTTGAGCCACTTAAATATTGAATTGCCTCTGGTTAAATTTGATGCTATGCTTGCA
AAATATTTGATTTCTACAACGGAAGATAATAAAATTTCAACCATTGCACGACTTTTTGATGTGGGACATTTGGCCACTGA
TGAGGAAATTTTTGGCAAAGGTACAAAACTTGCTCTACCTGATGATGAGATTTTGTTTGACCATTTGGCTCGTAAAATCA
GAGTTTTAGCTCGTGCCAAAGAAAAAATGATGGCCGAACTTATTGAAAATGAACAAGAACATCTTTTGTCTGATATGGAA
TTACCATTAGCAGAAGTTTTGGCAAAAATGGAGATTACAGGAATTTCTGTTAGTCAAAATACCTTGGAAGAAATTGGCGC
AGAAAACGAAGAAAAATTGGCTTCGTTGACTCGTGAAATTTATGATTTGGCTGGTGAAGAATTTAATATTAATTCGCCAA
AACAGTTGGGTGTGATTTTATTTGAAAAATTACAATTGCCAGTAGGTAAGAAAACAAAAACGGGTTATTCAACGGCGGTG
GATGTTTTAGAAGACTTGGCTGCATTATCTCCTGTGGTGGCAAAGATTTTGGAATATCGTCAAATCAATAAAGTACAATC
GACTTATGTTAAAGGCTTGATTCCGCAAATTGCTGATGATGGAAAAATCCATACGCGATATGTTCAGGACTTGACTCAGA
CCGGTCGTTTGAGTTCGGTTGACCCGAATCTGCAAAATATTCCTGTTCGACTGGAAGAAGGACGGAAGATTCGTAAGGCA
TTTGTGCCAAGTCAAGATTCTCTTTTGTTGAGTTCCGACTATTCACAAATTGAATTGCGAGTGCTGGCTCATATTTCTGC
TGATGAGCATTTGATTGACGCCTTTAAACATGGGGCTGATATCCATACGTCAACGGCTATGCGGGTCTTTGGCATTGAAA
AAGCAGAAGATGTCACGGCAAATGACCGCCGAAACGCAAAAGCAGTCAATTTTGGTGTGGTTTATGGAATTTCGGATTTT
GGTTTGGCAAGAAATCTGGGAATTACAAGAAAAGATGCCAAAAATTATATTGAAACTTACTTTGAACGTTATCCTGGTAT
TAAAACTTATATGGAAAATATTGTTCGCGAGGCTAGAGATAAAGGATTTGTTGAGACAATGAGTCATCGTCGTCGTAAGA
TTCCAGATATTAATGCACGTAATTTTAATGTTCGTGGTTTTGCGGAGCGGACGGCAATTAATTCGCCAATCCAAGGATCG
GCAGCGGATATTTTGAAAATTGCGATGATTAATCTGGATAAAGCGCTGACGGAACGTCAATCAAAATCAAAATTATTATT
GCAAGTACACGATGAAATTATTCTAGATGTTCCACTCGAGGAATTGGAGGATATCAAGGCACTTGTAAAGCAAACAATGG
AAGAAGCAATTGAACTTGCTGTGCCATTGAAAGTGGATGATAATACTGGTAAAACTTGGTATGAAGCCAAATAA

Upstream 100 bases:

>100_bases
AAAGCTGATTTTTATTGATTTGAGAGATAAATTGATTTGAATTAAAAGTAAAATAGAATAAATAGAATAAAAAGTAGAAA
AATAGAGGTAGATAATAGAG

Downstream 100 bases:

>100_bases
TACTAGTTATTAATGATACTGACAAGCTTTCTGTCAGCAAGTAGCAGTTGAATTTTTAATTCAGTTGACAGAGCAGCTAG
CTGACAAAAAAAGGTAAATA

Product: DNA polymerase I

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 877; Mature: 877

Protein sequence:

>877_residues
MEDKNRLLLIDGSSVAFRAFFALYNQLDRFKAPNGLHTNAIFAFHTMLSSLMERIQPTHVLIAFDAGKTTFRTEMFADYK
GGRSKTPDEFREQLPFIKEMIEKLGIRHYELANYEADDIIGTLDKMAEAPDVNFDVTIVTGDKDMIQLVDGNTRVEISKK
GVAEFEEFTPDYLLEKMGLTPSQFIDLKALMGDSSDNYPGVTKVGEKTGLKLLQEFGSLENLYENVETLKASKMKDNLIA
DKEMAFLSQQLATINTKAPLEIGLEDTLLKEKNVAELGQFYDEMGFSQFKSKLLADGGGEVTDETVSEEIKFEIVTDKSS
VASVNADDFFYLETLGENYHREQIVAFAWGNSEKIYVSKNLDLLTEMKFPENTYDFKKNRVLLSHLNIELPLVKFDAMLA
KYLISTTEDNKISTIARLFDVGHLATDEEIFGKGTKLALPDDEILFDHLARKIRVLARAKEKMMAELIENEQEHLLSDME
LPLAEVLAKMEITGISVSQNTLEEIGAENEEKLASLTREIYDLAGEEFNINSPKQLGVILFEKLQLPVGKKTKTGYSTAV
DVLEDLAALSPVVAKILEYRQINKVQSTYVKGLIPQIADDGKIHTRYVQDLTQTGRLSSVDPNLQNIPVRLEEGRKIRKA
FVPSQDSLLLSSDYSQIELRVLAHISADEHLIDAFKHGADIHTSTAMRVFGIEKAEDVTANDRRNAKAVNFGVVYGISDF
GLARNLGITRKDAKNYIETYFERYPGIKTYMENIVREARDKGFVETMSHRRRKIPDINARNFNVRGFAERTAINSPIQGS
AADILKIAMINLDKALTERQSKSKLLLQVHDEIILDVPLEELEDIKALVKQTMEEAIELAVPLKVDDNTGKTWYEAK

Sequences:

>Translated_877_residues
MEDKNRLLLIDGSSVAFRAFFALYNQLDRFKAPNGLHTNAIFAFHTMLSSLMERIQPTHVLIAFDAGKTTFRTEMFADYK
GGRSKTPDEFREQLPFIKEMIEKLGIRHYELANYEADDIIGTLDKMAEAPDVNFDVTIVTGDKDMIQLVDGNTRVEISKK
GVAEFEEFTPDYLLEKMGLTPSQFIDLKALMGDSSDNYPGVTKVGEKTGLKLLQEFGSLENLYENVETLKASKMKDNLIA
DKEMAFLSQQLATINTKAPLEIGLEDTLLKEKNVAELGQFYDEMGFSQFKSKLLADGGGEVTDETVSEEIKFEIVTDKSS
VASVNADDFFYLETLGENYHREQIVAFAWGNSEKIYVSKNLDLLTEMKFPENTYDFKKNRVLLSHLNIELPLVKFDAMLA
KYLISTTEDNKISTIARLFDVGHLATDEEIFGKGTKLALPDDEILFDHLARKIRVLARAKEKMMAELIENEQEHLLSDME
LPLAEVLAKMEITGISVSQNTLEEIGAENEEKLASLTREIYDLAGEEFNINSPKQLGVILFEKLQLPVGKKTKTGYSTAV
DVLEDLAALSPVVAKILEYRQINKVQSTYVKGLIPQIADDGKIHTRYVQDLTQTGRLSSVDPNLQNIPVRLEEGRKIRKA
FVPSQDSLLLSSDYSQIELRVLAHISADEHLIDAFKHGADIHTSTAMRVFGIEKAEDVTANDRRNAKAVNFGVVYGISDF
GLARNLGITRKDAKNYIETYFERYPGIKTYMENIVREARDKGFVETMSHRRRKIPDINARNFNVRGFAERTAINSPIQGS
AADILKIAMINLDKALTERQSKSKLLLQVHDEIILDVPLEELEDIKALVKQTMEEAIELAVPLKVDDNTGKTWYEAK
>Mature_877_residues
MEDKNRLLLIDGSSVAFRAFFALYNQLDRFKAPNGLHTNAIFAFHTMLSSLMERIQPTHVLIAFDAGKTTFRTEMFADYK
GGRSKTPDEFREQLPFIKEMIEKLGIRHYELANYEADDIIGTLDKMAEAPDVNFDVTIVTGDKDMIQLVDGNTRVEISKK
GVAEFEEFTPDYLLEKMGLTPSQFIDLKALMGDSSDNYPGVTKVGEKTGLKLLQEFGSLENLYENVETLKASKMKDNLIA
DKEMAFLSQQLATINTKAPLEIGLEDTLLKEKNVAELGQFYDEMGFSQFKSKLLADGGGEVTDETVSEEIKFEIVTDKSS
VASVNADDFFYLETLGENYHREQIVAFAWGNSEKIYVSKNLDLLTEMKFPENTYDFKKNRVLLSHLNIELPLVKFDAMLA
KYLISTTEDNKISTIARLFDVGHLATDEEIFGKGTKLALPDDEILFDHLARKIRVLARAKEKMMAELIENEQEHLLSDME
LPLAEVLAKMEITGISVSQNTLEEIGAENEEKLASLTREIYDLAGEEFNINSPKQLGVILFEKLQLPVGKKTKTGYSTAV
DVLEDLAALSPVVAKILEYRQINKVQSTYVKGLIPQIADDGKIHTRYVQDLTQTGRLSSVDPNLQNIPVRLEEGRKIRKA
FVPSQDSLLLSSDYSQIELRVLAHISADEHLIDAFKHGADIHTSTAMRVFGIEKAEDVTANDRRNAKAVNFGVVYGISDF
GLARNLGITRKDAKNYIETYFERYPGIKTYMENIVREARDKGFVETMSHRRRKIPDINARNFNVRGFAERTAINSPIQGS
AADILKIAMINLDKALTERQSKSKLLLQVHDEIILDVPLEELEDIKALVKQTMEEAIELAVPLKVDDNTGKTWYEAK

Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity [H]

COG id: COG0749

COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Homo sapiens, GI112420958, Length=462, Percent_Identity=33.982683982684, Blast_Score=232, Evalue=1e-60,
Organism=Homo sapiens, GI139394648, Length=524, Percent_Identity=30.9160305343511, Blast_Score=212, Evalue=1e-54,
Organism=Escherichia coli, GI1790294, Length=935, Percent_Identity=37.3262032085562, Blast_Score=534, Evalue=1e-152,
Organism=Escherichia coli, GI226510970, Length=224, Percent_Identity=30.8035714285714, Blast_Score=110, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI86563272, Length=408, Percent_Identity=28.4313725490196, Blast_Score=172, Evalue=5e-43,
Organism=Drosophila melanogaster, GI17933644, Length=415, Percent_Identity=32.7710843373494, Blast_Score=220, Evalue=4e-57,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 98867; Mature: 98867

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00447 DNA_POLYMERASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDKNRLLLIDGSSVAFRAFFALYNQLDRFKAPNGLHTNAIFAFHTMLSSLMERIQPTHV
CCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEE
LIAFDAGKTTFRTEMFADYKGGRSKTPDEFREQLPFIKEMIEKLGIRHYELANYEADDII
EEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHH
GTLDKMAEAPDVNFDVTIVTGDKDMIQLVDGNTRVEISKKGVAEFEEFTPDYLLEKMGLT
HHHHHHHCCCCCCEEEEEEECCCCEEEEECCCCEEEEECCCHHHHHHCCHHHHHHHHCCC
PSQFIDLKALMGDSSDNYPGVTKVGEKTGLKLLQEFGSLENLYENVETLKASKMKDNLIA
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DKEMAFLSQQLATINTKAPLEIGLEDTLLKEKNVAELGQFYDEMGFSQFKSKLLADGGGE
HHHHHHHHHHHHHCCCCCCEEECCHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCC
VTDETVSEEIKFEIVTDKSSVASVNADDFFYLETLGENYHREQIVAFAWGNSEKIYVSKN
CCHHHHCCCEEEEEEECCCCCCCCCCCCEEEHHHHCCCCCCCEEEEEEECCCCEEEEECC
LDLLTEMKFPENTYDFKKNRVLLSHLNIELPLVKFDAMLAKYLISTTEDNKISTIARLFD
CCHHEECCCCCCCCCCCCCCEEEEECCEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
VGHLATDEEIFGKGTKLALPDDEILFDHLARKIRVLARAKEKMMAELIENEQEHLLSDME
HCCCCCCHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCC
LPLAEVLAKMEITGISVSQNTLEEIGAENEEKLASLTREIYDLAGEEFNINSPKQLGVIL
CCHHHHHHHHHHCCEECCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
FEKLQLPVGKKTKTGYSTAVDVLEDLAALSPVVAKILEYRQINKVQSTYVKGLIPQIADD
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GKIHTRYVQDLTQTGRLSSVDPNLQNIPVRLEEGRKIRKAFVPSQDSLLLSSDYSQIELR
CCCHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCHHHHHHCCCCCCEEEECCCCCEEEE
VLAHISADEHLIDAFKHGADIHTSTAMRVFGIEKAEDVTANDRRNAKAVNFGVVYGISDF
EEEECCCCHHHHHHHHCCCCCCCCHHHHEEECCCCCCCCCCCCCCCEEEEEEEEECCCHH
GLARNLGITRKDAKNYIETYFERYPGIKTYMENIVREARDKGFVETMSHRRRKIPDINAR
CHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCC
NFNVRGFAERTAINSPIQGSAADILKIAMINLDKALTERQSKSKLLLQVHDEIILDVPLE
CCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHEEECCHH
ELEDIKALVKQTMEEAIELAVPLKVDDNTGKTWYEAK
HHHHHHHHHHHHHHHHHHEEEEEEEECCCCCEEECCC
>Mature Secondary Structure
MEDKNRLLLIDGSSVAFRAFFALYNQLDRFKAPNGLHTNAIFAFHTMLSSLMERIQPTHV
CCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEE
LIAFDAGKTTFRTEMFADYKGGRSKTPDEFREQLPFIKEMIEKLGIRHYELANYEADDII
EEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHH
GTLDKMAEAPDVNFDVTIVTGDKDMIQLVDGNTRVEISKKGVAEFEEFTPDYLLEKMGLT
HHHHHHHCCCCCCEEEEEEECCCCEEEEECCCCEEEEECCCHHHHHHCCHHHHHHHHCCC
PSQFIDLKALMGDSSDNYPGVTKVGEKTGLKLLQEFGSLENLYENVETLKASKMKDNLIA
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DKEMAFLSQQLATINTKAPLEIGLEDTLLKEKNVAELGQFYDEMGFSQFKSKLLADGGGE
HHHHHHHHHHHHHCCCCCCEEECCHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCC
VTDETVSEEIKFEIVTDKSSVASVNADDFFYLETLGENYHREQIVAFAWGNSEKIYVSKN
CCHHHHCCCEEEEEEECCCCCCCCCCCCEEEHHHHCCCCCCCEEEEEEECCCCEEEEECC
LDLLTEMKFPENTYDFKKNRVLLSHLNIELPLVKFDAMLAKYLISTTEDNKISTIARLFD
CCHHEECCCCCCCCCCCCCCEEEEECCEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
VGHLATDEEIFGKGTKLALPDDEILFDHLARKIRVLARAKEKMMAELIENEQEHLLSDME
HCCCCCCHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCC
LPLAEVLAKMEITGISVSQNTLEEIGAENEEKLASLTREIYDLAGEEFNINSPKQLGVIL
CCHHHHHHHHHHCCEECCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
FEKLQLPVGKKTKTGYSTAVDVLEDLAALSPVVAKILEYRQINKVQSTYVKGLIPQIADD
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GKIHTRYVQDLTQTGRLSSVDPNLQNIPVRLEEGRKIRKAFVPSQDSLLLSSDYSQIELR
CCCHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCHHHHHHCCCCCCEEEECCCCCEEEE
VLAHISADEHLIDAFKHGADIHTSTAMRVFGIEKAEDVTANDRRNAKAVNFGVVYGISDF
EEEECCCCHHHHHHHHCCCCCCCCHHHHEEECCCCCCCCCCCCCCCEEEEEEEEECCCHH
GLARNLGITRKDAKNYIETYFERYPGIKTYMENIVREARDKGFVETMSHRRRKIPDINAR
CHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCC
NFNVRGFAERTAINSPIQGSAADILKIAMINLDKALTERQSKSKLLLQVHDEIILDVPLE
CCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHEEECCHH
ELEDIKALVKQTMEEAIELAVPLKVDDNTGKTWYEAK
HHHHHHHHHHHHHHHHHHEEEEEEEECCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]