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The map label for this gene is proS [H]

Identifier: 125625170

GI number: 125625170

Start: 2354322

End: 2356172

Strand: Reverse

Name: proS [H]

Synonym: llmg_2412

Alternate gene names: 125625170

Gene position: 2356172-2354322 (Counterclockwise)

Preceding gene: 125625171

Following gene: 125625169

Centisome position: 93.15

GC content: 37.55

Gene sequence:

>1851_bases
ATGAAACAATCAAAAATGCTTATACCAACCCTTCGAGAAATGCCTTCTGATGCCCAGGTTATTAGCCATGCTTTACTGAT
GCGTGCAGGATATGTTCGCCAAGTTTCAGCAGGGATTTATGCCTACTTGCCATTGGCAAATCGTGTACTTGAAAAGTTGA
AAAATATCATGCGTGAAGAGTTTGATGAAATTGGTGCTGTAGAACTTCTTGCTCCAAGTCTTTTGACAGCAGACCTTTGG
CGTGAATCTGGTCGTTACGAAACTTACGGAGAAGATTTATATAAATTAAAAAACCGTGATAGTTCTGATTTCATTTTAGG
ACCAACTCATGAGGAAACAATGACTTCTCTTGTCCGTGACGAAATCACTTCTTATAAAAAATTACCACTCAATGTTTACC
AAATTGCGACAAAATTCCGTGATGAAAAACGTCCGCGTTATGGTTTATTGCGTGGTCGTGAGTTCTTGATGAAAGATGGT
TATAGTTACCATGCTGACCAAGATTCATTAGATGAAACTTATCTTGATTATAAAAAAGCTTACGAAAAAATCTTTGAGCG
TGCTGGTTTGAACTTTAAACCAATTATTGCCGATGCTGGTGCAATGGGTGGTAAAGATAGTCAAGAGTTTATTGCGATTA
CTGATGACCGCATCAATCTCGAAAAATGGCTTGTTTTAAGTAAAAATATTACTTCAATTGATGAAATTCCAGAATCTGTC
CTTTCAGAAATCCAAGAAGAACTTGGTAAATGGTTGGTTGCTGGTGAAGATACCATTGTTTATGCTGAAGGTGGCGATTA
CGCTGCAAACATTGAAATGGCAACAAGCCAGTTTGAACCAAATGTCGCTTATACAGAAGAACTTGAACTTGAAAAAGTAG
CAACACCAGGGGCTAAAACAATTGATGAAGTATCAGACTTCCTTGAAATTGATGAAGAACAAACGGTTAAAACTTTGGTT
TATCATGCTGATGATGAGTTAATTGTTATTTTGTTAAACGGGAATGACCAACTTAATGAAGTGAAATTAACTAACCATCT
TGGAGCGTCATTTATTGAAGCTGCAAGTGAAGCAGAAGTTGAAGAAAAATTTGGAGCTCATTTTGGTTCACTTGGACCTA
TTGGTCTTGAAAATGTTCGAATTATTGCTGACCGCAAAGTTGAGTTAATTAAAAATGCAGTGGTTGGTGCGAATGTTGAT
GGCTATCATTACAAGAATGCTAATTTCGGTCGTGATTTTGAAGTTGAAGAATTTGTTGATTTACGCACAGTTAATGAAGG
TGAAATTTCACCAGATGGCCGTGGAACTTTGAAATTTGCACGTGGGATTGAAATTGGCCACATCTTTAAATTGGGCACTC
GCTATACAGAAGCCATGAATGCCAATATTCTTGATGCCAATGGTCGTTCAATTCCAATGCTCATGGGCTGCTACGGAATC
GGTGTCAGTCGTTTGCTTTCAGCTATTTTGGAACAATTTGCCCGTATCTATGTTGAAAAAACACCAAGAGAAGAATTCAA
ATTTAGCTGGTCAATCAATTTCCCTAAAGAATTGGCACCATTTGACATTCACCTTGTACCTGTCAATGTTAAAGATGAAG
CAGCAATGGAATTAACTTCTGAACTTGAAGAAAAACTCCGTGGTAAAGGTTACCAAGTTTTAGTTGATGACCGTAATGAA
CGTGCAGGGGTGAAATTTGCTGATAGTGATTTGATTGGACTTCCAGTCCGTGTAACAATCGGTAAAAAAGCAGCTGAGGG
AATCGTTGAAGTTAAAATTCGTGCGACAGGTGAAGTTGTCGAAATCAACAAAGATGAACTTGTTAATACCATTGAAATTT
TAAGTAAATAA

Upstream 100 bases:

>100_bases
GAATGACATTCTACGGGCTTTTGTCAACTAATATTATCTATTCGTCTTCTATTCAGAAAAAAAGAAGTTGAACCATACTC
ATAAAGGAAATTTTAAAATT

Downstream 100 bases:

>100_bases
AAAAGAGATTTACGATGAAAATTGTTATTGTTGGAAGCTCCCATGCCGGAATTTGTGCTGGTTTACGGGCGCTTGAAGAA
TACCCAGAAGCCGAGATTAC

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 616; Mature: 616

Protein sequence:

>616_residues
MKQSKMLIPTLREMPSDAQVISHALLMRAGYVRQVSAGIYAYLPLANRVLEKLKNIMREEFDEIGAVELLAPSLLTADLW
RESGRYETYGEDLYKLKNRDSSDFILGPTHEETMTSLVRDEITSYKKLPLNVYQIATKFRDEKRPRYGLLRGREFLMKDG
YSYHADQDSLDETYLDYKKAYEKIFERAGLNFKPIIADAGAMGGKDSQEFIAITDDRINLEKWLVLSKNITSIDEIPESV
LSEIQEELGKWLVAGEDTIVYAEGGDYAANIEMATSQFEPNVAYTEELELEKVATPGAKTIDEVSDFLEIDEEQTVKTLV
YHADDELIVILLNGNDQLNEVKLTNHLGASFIEAASEAEVEEKFGAHFGSLGPIGLENVRIIADRKVELIKNAVVGANVD
GYHYKNANFGRDFEVEEFVDLRTVNEGEISPDGRGTLKFARGIEIGHIFKLGTRYTEAMNANILDANGRSIPMLMGCYGI
GVSRLLSAILEQFARIYVEKTPREEFKFSWSINFPKELAPFDIHLVPVNVKDEAAMELTSELEEKLRGKGYQVLVDDRNE
RAGVKFADSDLIGLPVRVTIGKKAAEGIVEVKIRATGEVVEINKDELVNTIEILSK

Sequences:

>Translated_616_residues
MKQSKMLIPTLREMPSDAQVISHALLMRAGYVRQVSAGIYAYLPLANRVLEKLKNIMREEFDEIGAVELLAPSLLTADLW
RESGRYETYGEDLYKLKNRDSSDFILGPTHEETMTSLVRDEITSYKKLPLNVYQIATKFRDEKRPRYGLLRGREFLMKDG
YSYHADQDSLDETYLDYKKAYEKIFERAGLNFKPIIADAGAMGGKDSQEFIAITDDRINLEKWLVLSKNITSIDEIPESV
LSEIQEELGKWLVAGEDTIVYAEGGDYAANIEMATSQFEPNVAYTEELELEKVATPGAKTIDEVSDFLEIDEEQTVKTLV
YHADDELIVILLNGNDQLNEVKLTNHLGASFIEAASEAEVEEKFGAHFGSLGPIGLENVRIIADRKVELIKNAVVGANVD
GYHYKNANFGRDFEVEEFVDLRTVNEGEISPDGRGTLKFARGIEIGHIFKLGTRYTEAMNANILDANGRSIPMLMGCYGI
GVSRLLSAILEQFARIYVEKTPREEFKFSWSINFPKELAPFDIHLVPVNVKDEAAMELTSELEEKLRGKGYQVLVDDRNE
RAGVKFADSDLIGLPVRVTIGKKAAEGIVEVKIRATGEVVEINKDELVNTIEILSK
>Mature_616_residues
MKQSKMLIPTLREMPSDAQVISHALLMRAGYVRQVSAGIYAYLPLANRVLEKLKNIMREEFDEIGAVELLAPSLLTADLW
RESGRYETYGEDLYKLKNRDSSDFILGPTHEETMTSLVRDEITSYKKLPLNVYQIATKFRDEKRPRYGLLRGREFLMKDG
YSYHADQDSLDETYLDYKKAYEKIFERAGLNFKPIIADAGAMGGKDSQEFIAITDDRINLEKWLVLSKNITSIDEIPESV
LSEIQEELGKWLVAGEDTIVYAEGGDYAANIEMATSQFEPNVAYTEELELEKVATPGAKTIDEVSDFLEIDEEQTVKTLV
YHADDELIVILLNGNDQLNEVKLTNHLGASFIEAASEAEVEEKFGAHFGSLGPIGLENVRIIADRKVELIKNAVVGANVD
GYHYKNANFGRDFEVEEFVDLRTVNEGEISPDGRGTLKFARGIEIGHIFKLGTRYTEAMNANILDANGRSIPMLMGCYGI
GVSRLLSAILEQFARIYVEKTPREEFKFSWSINFPKELAPFDIHLVPVNVKDEAAMELTSELEEKLRGKGYQVLVDDRNE
RAGVKFADSDLIGLPVRVTIGKKAAEGIVEVKIRATGEVVEINKDELVNTIEILSK

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=229, Percent_Identity=37.5545851528384, Blast_Score=174, Evalue=2e-43,
Organism=Escherichia coli, GI1786392, Length=617, Percent_Identity=42.1393841166937, Blast_Score=471, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI115532348, Length=198, Percent_Identity=37.3737373737374, Blast_Score=146, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6320931, Length=584, Percent_Identity=27.0547945205479, Blast_Score=187, Evalue=6e-48,
Organism=Drosophila melanogaster, GI24656200, Length=217, Percent_Identity=34.1013824884793, Blast_Score=140, Evalue=2e-33,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 69243; Mature: 69243

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQSKMLIPTLREMPSDAQVISHALLMRAGYVRQVSAGIYAYLPLANRVLEKLKNIMREE
CCCCCCCCHHHHHCCCHHHHHHHHHHHHHCHHHHHHCCCEEEHHHHHHHHHHHHHHHHHH
FDEIGAVELLAPSLLTADLWRESGRYETYGEDLYKLKNRDSSDFILGPTHEETMTSLVRD
HHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHH
EITSYKKLPLNVYQIATKFRDEKRPRYGLLRGREFLMKDGYSYHADQDSLDETYLDYKKA
HHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
YEKIFERAGLNFKPIIADAGAMGGKDSQEFIAITDDRINLEKWLVLSKNITSIDEIPESV
HHHHHHHCCCCCEEEEECCCCCCCCCCCCEEEEECCCCCHHHEEEECCCCCHHHHHHHHH
LSEIQEELGKWLVAGEDTIVYAEGGDYAANIEMATSQFEPNVAYTEELELEKVATPGAKT
HHHHHHHHCCEEEECCCEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCHHHHCCCCCHH
IDEVSDFLEIDEEQTVKTLVYHADDELIVILLNGNDQLNEVKLTNHLGASFIEAASEAEV
HHHHHHHHHCCCHHHEEEHEEECCCCEEEEEECCCCCCCEEEEHHHHCHHHHHHHHHHHH
EEKFGAHFGSLGPIGLENVRIIADRKVELIKNAVVGANVDGYHYKNANFGRDFEVEEFVD
HHHHCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHH
LRTVNEGEISPDGRGTLKFARGIEIGHIFKLGTRYTEAMNANILDANGRSIPMLMGCYGI
HEECCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHH
GVSRLLSAILEQFARIYVEKTPREEFKFSWSINFPKELAPFDIHLVPVNVKDEAAMELTS
HHHHHHHHHHHHHHHHHHCCCCHHHEEEEEEECCCCCCCCEEEEEEEECCCCHHHHHHHH
ELEEKLRGKGYQVLVDDRNERAGVKFADSDLIGLPVRVTIGKKAAEGIVEVKIRATGEVV
HHHHHHCCCCCEEEEECCCCCCCCEEECCCEECCEEEEEECCHHHCCEEEEEEEECCCEE
EINKDELVNTIEILSK
ECCHHHHHHHHHHHCC
>Mature Secondary Structure
MKQSKMLIPTLREMPSDAQVISHALLMRAGYVRQVSAGIYAYLPLANRVLEKLKNIMREE
CCCCCCCCHHHHHCCCHHHHHHHHHHHHHCHHHHHHCCCEEEHHHHHHHHHHHHHHHHHH
FDEIGAVELLAPSLLTADLWRESGRYETYGEDLYKLKNRDSSDFILGPTHEETMTSLVRD
HHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHH
EITSYKKLPLNVYQIATKFRDEKRPRYGLLRGREFLMKDGYSYHADQDSLDETYLDYKKA
HHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
YEKIFERAGLNFKPIIADAGAMGGKDSQEFIAITDDRINLEKWLVLSKNITSIDEIPESV
HHHHHHHCCCCCEEEEECCCCCCCCCCCCEEEEECCCCCHHHEEEECCCCCHHHHHHHHH
LSEIQEELGKWLVAGEDTIVYAEGGDYAANIEMATSQFEPNVAYTEELELEKVATPGAKT
HHHHHHHHCCEEEECCCEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCHHHHCCCCCHH
IDEVSDFLEIDEEQTVKTLVYHADDELIVILLNGNDQLNEVKLTNHLGASFIEAASEAEV
HHHHHHHHHCCCHHHEEEHEEECCCCEEEEEECCCCCCCEEEEHHHHCHHHHHHHHHHHH
EEKFGAHFGSLGPIGLENVRIIADRKVELIKNAVVGANVDGYHYKNANFGRDFEVEEFVD
HHHHCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHH
LRTVNEGEISPDGRGTLKFARGIEIGHIFKLGTRYTEAMNANILDANGRSIPMLMGCYGI
HEECCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHH
GVSRLLSAILEQFARIYVEKTPREEFKFSWSINFPKELAPFDIHLVPVNVKDEAAMELTS
HHHHHHHHHHHHHHHHHHCCCCHHHEEEEEEECCCCCCCCEEEEEEEECCCCHHHHHHHH
ELEEKLRGKGYQVLVDDRNERAGVKFADSDLIGLPVRVTIGKKAAEGIVEVKIRATGEVV
HHHHHHCCCCCEEEEECCCCCCCCEEECCCEECCEEEEEECCHHHCCEEEEEEEECCCEE
EINKDELVNTIEILSK
ECCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]