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The map label for this gene is pbp2A [H]

Identifier: 125625152

GI number: 125625152

Start: 2333678

End: 2335909

Strand: Reverse

Name: pbp2A [H]

Synonym: llmg_2392

Alternate gene names: 125625152

Gene position: 2335909-2333678 (Counterclockwise)

Preceding gene: 125625153

Following gene: 125625151

Centisome position: 92.35

GC content: 37.72

Gene sequence:

>2232_bases
ATGCCTGAAAATAAGAATTTCAGTCGGCGTAGTAAAAAAGAAACAGGAAAGAAAAGTTTAGAAATTCCTAAAATACGCCC
TAAAAAACAAAAAAAACTTGAAGAAGAAATAGAAAAACCAGCTAAAACTAAATTTGGTAAATTTATGAGACCGATAAAAC
GTTTCTGGAAACGTTATAACTTAACAAAAATTACGATTATTTTTGTTTTGGTAGCGATCGTTGCGACAGGCTCATATCTT
TTTTACTTGGCTAAAACAGCAAATGTGAAGGTTCTACAAAGTTCTATTTCTGCTCAGACAGTTATTTATGATAAAGATAA
TAATGAAGCTGGAAATCTTTATGGCCAAAAGGGAACTCCTGTTAAGATTGACCAAATTTCTAAAAACATTACGAATGCTG
TAGTGGCAACAGAAGATAGAACCTTTTATGAAAACCACGGGGTTAATTTGAAACGTTTTGCTCTGGCGGCAGTAACACTT
GGTCGTTTTGGTGGTGGTTCAACAATTACTCAACAGTTGGCTAAAAATGCTTACTTGACTCAAGAACAAACCATTGACCG
TAAGGCGCGTGAATTTTTCTTGGCTCTTGAAATTAACAAACACTATAGCAAAGATGAGATTTTAGATATGTATTTGAATA
ACTCTTACTTTGGTAATGGGGTTTGGGGAATTCAAGATGCAGCCTTGAAATATTTTGGAGTTCCAGCCAGTGAAGTAACT
GTTGATGAAGCAGCCAGTCTTGCCGGAATGCTAAAAGGTCCTGAAATTTATAATCCTTTATATGAAAAGGGAAAATATGC
CACCGACCGTCGCAATACCGTCTTACAAAATATGGTTAATGCTGGCTATCTTGAGCAATCACAAGCAGATAGCTTTATGA
AGGTTGATTTACAAGCTCAATTGCAAGATAATTATCAATCTAAAAGTAGTCAGTACAAATATCCAAGCTACTATAATGCG
GTAATTTCTGAGGCTGAACGTAAGTATGGTTTAACCTTACAAGAAATTATGAATAATGGCTATAAAATTTATACGGGAAT
GGATCAAAATATGCAGTCAGGTTTACAAAAAACCTATGCGGATCCAAGCTTCTTCCCACAAGCAGCAGATGGAACCTATG
CCCAGTCAGCATCGGTGGCAATTGATCCAAAAACAGGAGCTGTTAATGCTCTCGTGGGTAATGTTAATACAGAAGGTTCT
AATAGTTTTACTGATTATAACTATGCGACAATGTCTAAACGTTCTCCCGGTTCGGTCATCAAACCATTGATTGTCTATGC
TCCTGCAATTGAAGCGGGTTGGTCAATTGACAAAACAGTTGATGACTCTCCGGCTGATTATAATGGTTGGAAACCAACGG
ACTTTGATAATCAATGGCGAGGACAAATTCCGATGTATACGGCTTTAGCCAATTCATATAATATTCCAGCCATAAATACC
TATCAAGCCATTGGTCCAAAAGTTGGAAATGCTTTGGGACGTGAATTTGGTTTGGATTTAAATAGTAAAAATGATGTTTT
ACCAACGGCTCTTGGTGCAGGAGTTGAAACAAATCCATGGCAAATTGCTCAGGCTTATCAAGCTTTTGCCAATGGTGGAG
TAATGAATGATGCGCATCTCATTACTAAAATTGAAAATGCGGCTGGTCAAGTCGTTAAAACGGCAAAAGTAAACAAAAAA
CGTGTCGTTAGTAAAGATACTGCCGATAAAATGACTCAAATGATGTTAGGGACTTACACTAATGGTTCTGCTTGGAAAGC
TTCACCTAAATCTTATACTTTGGCAGGTAAGACAGGGACTAACGAAGACCAAGACCAGTGGGTAGTCGGTTATACTCCAG
ATGTTGTGATGGCGCTTTGGGTTGGTTATTCTGATGGTAAATATAAATTGACAGGTTCATCAGAAGGACAAACGTCAGTA
ATTTTCCGTCAGGAAGCAAGTTATATGTTGCCTTACACCAAAGGAACACCTTTCACAGTTGAAAACCCTTATGCTCAGGC
AGGCGTTGCAGCTCAACAACCTTATTGGACTCAACAAAGACAATATCAAGATGATATTGTAGACCAAGAACAGGCAGAAG
CGAATACGACTGGAAATACTCCCGAAAGCTCTAGTTCTAGCACTTCATCATCTTCTGATTCCAATGGTTTGGACCTAGGT
AAAATTGGTAAAAATATTGGTGATGCAGCTAAAAATGCTTGGGATAAAGTTAAAGGGGTTTTTGGCAACTAA

Upstream 100 bases:

>100_bases
CAAATATGTAAGGAAAAAAAATCGGAAAAGTATTAAAATAAGATATTGATGTATTCATTTGAGTAAGTATAGAAATAAAA
AATATCTGAGGGAAGTATAG

Downstream 100 bases:

>100_bases
AAGTAAAAAAAACACTGAAAATATGATAAAATTAAGAAGAAAGCGTAGTAGTTTTAAGACAAAAAGAACTTAAAGAATAA
AGATAGAAATCGAGAAGGAA

Product: penicillin-binding protein 2a

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 743; Mature: 742

Protein sequence:

>743_residues
MPENKNFSRRSKKETGKKSLEIPKIRPKKQKKLEEEIEKPAKTKFGKFMRPIKRFWKRYNLTKITIIFVLVAIVATGSYL
FYLAKTANVKVLQSSISAQTVIYDKDNNEAGNLYGQKGTPVKIDQISKNITNAVVATEDRTFYENHGVNLKRFALAAVTL
GRFGGGSTITQQLAKNAYLTQEQTIDRKAREFFLALEINKHYSKDEILDMYLNNSYFGNGVWGIQDAALKYFGVPASEVT
VDEAASLAGMLKGPEIYNPLYEKGKYATDRRNTVLQNMVNAGYLEQSQADSFMKVDLQAQLQDNYQSKSSQYKYPSYYNA
VISEAERKYGLTLQEIMNNGYKIYTGMDQNMQSGLQKTYADPSFFPQAADGTYAQSASVAIDPKTGAVNALVGNVNTEGS
NSFTDYNYATMSKRSPGSVIKPLIVYAPAIEAGWSIDKTVDDSPADYNGWKPTDFDNQWRGQIPMYTALANSYNIPAINT
YQAIGPKVGNALGREFGLDLNSKNDVLPTALGAGVETNPWQIAQAYQAFANGGVMNDAHLITKIENAAGQVVKTAKVNKK
RVVSKDTADKMTQMMLGTYTNGSAWKASPKSYTLAGKTGTNEDQDQWVVGYTPDVVMALWVGYSDGKYKLTGSSEGQTSV
IFRQEASYMLPYTKGTPFTVENPYAQAGVAAQQPYWTQQRQYQDDIVDQEQAEANTTGNTPESSSSSTSSSSDSNGLDLG
KIGKNIGDAAKNAWDKVKGVFGN

Sequences:

>Translated_743_residues
MPENKNFSRRSKKETGKKSLEIPKIRPKKQKKLEEEIEKPAKTKFGKFMRPIKRFWKRYNLTKITIIFVLVAIVATGSYL
FYLAKTANVKVLQSSISAQTVIYDKDNNEAGNLYGQKGTPVKIDQISKNITNAVVATEDRTFYENHGVNLKRFALAAVTL
GRFGGGSTITQQLAKNAYLTQEQTIDRKAREFFLALEINKHYSKDEILDMYLNNSYFGNGVWGIQDAALKYFGVPASEVT
VDEAASLAGMLKGPEIYNPLYEKGKYATDRRNTVLQNMVNAGYLEQSQADSFMKVDLQAQLQDNYQSKSSQYKYPSYYNA
VISEAERKYGLTLQEIMNNGYKIYTGMDQNMQSGLQKTYADPSFFPQAADGTYAQSASVAIDPKTGAVNALVGNVNTEGS
NSFTDYNYATMSKRSPGSVIKPLIVYAPAIEAGWSIDKTVDDSPADYNGWKPTDFDNQWRGQIPMYTALANSYNIPAINT
YQAIGPKVGNALGREFGLDLNSKNDVLPTALGAGVETNPWQIAQAYQAFANGGVMNDAHLITKIENAAGQVVKTAKVNKK
RVVSKDTADKMTQMMLGTYTNGSAWKASPKSYTLAGKTGTNEDQDQWVVGYTPDVVMALWVGYSDGKYKLTGSSEGQTSV
IFRQEASYMLPYTKGTPFTVENPYAQAGVAAQQPYWTQQRQYQDDIVDQEQAEANTTGNTPESSSSSTSSSSDSNGLDLG
KIGKNIGDAAKNAWDKVKGVFGN
>Mature_742_residues
PENKNFSRRSKKETGKKSLEIPKIRPKKQKKLEEEIEKPAKTKFGKFMRPIKRFWKRYNLTKITIIFVLVAIVATGSYLF
YLAKTANVKVLQSSISAQTVIYDKDNNEAGNLYGQKGTPVKIDQISKNITNAVVATEDRTFYENHGVNLKRFALAAVTLG
RFGGGSTITQQLAKNAYLTQEQTIDRKAREFFLALEINKHYSKDEILDMYLNNSYFGNGVWGIQDAALKYFGVPASEVTV
DEAASLAGMLKGPEIYNPLYEKGKYATDRRNTVLQNMVNAGYLEQSQADSFMKVDLQAQLQDNYQSKSSQYKYPSYYNAV
ISEAERKYGLTLQEIMNNGYKIYTGMDQNMQSGLQKTYADPSFFPQAADGTYAQSASVAIDPKTGAVNALVGNVNTEGSN
SFTDYNYATMSKRSPGSVIKPLIVYAPAIEAGWSIDKTVDDSPADYNGWKPTDFDNQWRGQIPMYTALANSYNIPAINTY
QAIGPKVGNALGREFGLDLNSKNDVLPTALGAGVETNPWQIAQAYQAFANGGVMNDAHLITKIENAAGQVVKTAKVNKKR
VVSKDTADKMTQMMLGTYTNGSAWKASPKSYTLAGKTGTNEDQDQWVVGYTPDVVMALWVGYSDGKYKLTGSSEGQTSVI
FRQEASYMLPYTKGTPFTVENPYAQAGVAAQQPYWTQQRQYQDDIVDQEQAEANTTGNTPESSSSSTSSSSDSNGLDLGK
IGKNIGDAAKNAWDKVKGVFGN

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=606, Percent_Identity=29.7029702970297, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI87082258, Length=303, Percent_Identity=35.973597359736, Blast_Score=168, Evalue=1e-42,
Organism=Escherichia coli, GI1788867, Length=574, Percent_Identity=27.0034843205575, Blast_Score=151, Evalue=2e-37,
Organism=Escherichia coli, GI1789601, Length=177, Percent_Identity=38.4180790960452, Blast_Score=100, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 81997; Mature: 81865

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPENKNFSRRSKKETGKKSLEIPKIRPKKQKKLEEEIEKPAKTKFGKFMRPIKRFWKRYN
CCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LTKITIIFVLVAIVATGSYLFYLAKTANVKVLQSSISAQTVIYDKDNNEAGNLYGQKGTP
CHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCC
VKIDQISKNITNAVVATEDRTFYENHGVNLKRFALAAVTLGRFGGGSTITQQLAKNAYLT
EEHHHHHHHHHHCEEEECCCEEHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCC
QEQTIDRKAREFFLALEINKHYSKDEILDMYLNNSYFGNGVWGIQDAALKYFGVPASEVT
HHHHHHHHHHEEEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHH
VDEAASLAGMLKGPEIYNPLYEKGKYATDRRNTVLQNMVNAGYLEQSQADSFMKVDLQAQ
HHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHCCCEEEEEHHHH
LQDNYQSKSSQYKYPSYYNAVISEAERKYGLTLQEIMNNGYKIYTGMDQNMQSGLQKTYA
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCEEEEECCCHHHHHHHHHHCC
DPSFFPQAADGTYAQSASVAIDPKTGAVNALVGNVNTEGSNSFTDYNYATMSKRSPGSVI
CCCCCCCCCCCCCCCCCCEEECCCCCCEEEEEECCCCCCCCCCCCCCEEECCCCCCCHHH
KPLIVYAPAIEAGWSIDKTVDDSPADYNGWKPTDFDNQWRGQIPMYTALANSYNIPAINT
CCHHEEECCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHCCCCCCCCCC
YQAIGPKVGNALGREFGLDLNSKNDVLPTALGAGVETNPWQIAQAYQAFANGGVMNDAHL
HHHHCCHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHCCCCCCCHHH
ITKIENAAGQVVKTAKVNKKRVVSKDTADKMTQMMLGTYTNGSAWKASPKSYTLAGKTGT
HHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCC
NEDQDQWVVGYTPDVVMALWVGYSDGKYKLTGSSEGQTSVIFRQEASYMLPYTKGTPFTV
CCCCCCEEEECCHHHHHHHHEECCCCEEEEECCCCCCEEEEEEECCCEEEEECCCCCEEE
ENPYAQAGVAAQQPYWTQQRQYQDDIVDQEQAEANTTGNTPESSSSSTSSSSDSNGLDLG
CCCHHHCCCCCCCCCCHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHH
KIGKNIGDAAKNAWDKVKGVFGN
HHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PENKNFSRRSKKETGKKSLEIPKIRPKKQKKLEEEIEKPAKTKFGKFMRPIKRFWKRYN
CCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LTKITIIFVLVAIVATGSYLFYLAKTANVKVLQSSISAQTVIYDKDNNEAGNLYGQKGTP
CHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCC
VKIDQISKNITNAVVATEDRTFYENHGVNLKRFALAAVTLGRFGGGSTITQQLAKNAYLT
EEHHHHHHHHHHCEEEECCCEEHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCC
QEQTIDRKAREFFLALEINKHYSKDEILDMYLNNSYFGNGVWGIQDAALKYFGVPASEVT
HHHHHHHHHHEEEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHH
VDEAASLAGMLKGPEIYNPLYEKGKYATDRRNTVLQNMVNAGYLEQSQADSFMKVDLQAQ
HHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHCCCEEEEEHHHH
LQDNYQSKSSQYKYPSYYNAVISEAERKYGLTLQEIMNNGYKIYTGMDQNMQSGLQKTYA
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCEEEEECCCHHHHHHHHHHCC
DPSFFPQAADGTYAQSASVAIDPKTGAVNALVGNVNTEGSNSFTDYNYATMSKRSPGSVI
CCCCCCCCCCCCCCCCCCEEECCCCCCEEEEEECCCCCCCCCCCCCCEEECCCCCCCHHH
KPLIVYAPAIEAGWSIDKTVDDSPADYNGWKPTDFDNQWRGQIPMYTALANSYNIPAINT
CCHHEEECCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHCCCCCCCCCC
YQAIGPKVGNALGREFGLDLNSKNDVLPTALGAGVETNPWQIAQAYQAFANGGVMNDAHL
HHHHCCHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHCCCCCCCHHH
ITKIENAAGQVVKTAKVNKKRVVSKDTADKMTQMMLGTYTNGSAWKASPKSYTLAGKTGT
HHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCC
NEDQDQWVVGYTPDVVMALWVGYSDGKYKLTGSSEGQTSVIFRQEASYMLPYTKGTPFTV
CCCCCCEEEECCHHHHHHHHEECCCCEEEEECCCCCCEEEEEEECCCEEEEECCCCCEEE
ENPYAQAGVAAQQPYWTQQRQYQDDIVDQEQAEANTTGNTPESSSSSTSSSSDSNGLDLG
CCCHHHCCCCCCCCCCHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHH
KIGKNIGDAAKNAWDKVKGVFGN
HHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]