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The map label for this gene is poxL [H]

Identifier: 125625082

GI number: 125625082

Start: 2277746

End: 2279473

Strand: Reverse

Name: poxL [H]

Synonym: llmg_2321

Alternate gene names: 125625082

Gene position: 2279473-2277746 (Counterclockwise)

Preceding gene: 125625083

Following gene: 125625081

Centisome position: 90.12

GC content: 39.64

Gene sequence:

>1728_bases
ATGACAAAAATAAATGCAGCAGATGCAATGTTAAAAGTTATTGAAGATTGGGGTGTCAAAAATATTTACGGTTTACCCGG
CGGATCAATTGATTCGACAATGAACGCATTATATAATCGAAAAGACACATTGAATTATATTCAAGTTCGCCATGAAGAAG
TGGGGGCTTTAGCGGCTGTCGGAGAAGCAAAAGTAACCGGAGAGATTGGTGTAACCTTTGGTTCGGCAGGACCAGGAGCC
GTTCATTTATTAAATGGCTTATATGACGCTCAATACGATCATGTTCCAGTCCTTGCCTTAGTTGGACAAGTTCCAACGAG
TGCAATGAACACTAATTATTTCCAAGAGTTAAACGAAAATCCAATGTTTGCTGATGTTAGTGTTTATAATCGAACAGTAA
TGACAGCAGAACAAATGCCAGCGGTAGTTGACGAAGCCATTCGTCAAGCCTATGCTAAACGTGGTGTTGCTGTCATTACC
ATTCCCAAAGATTTGGGTTGGCAAGAAATTGAAGACAACTATATTTCTAGTGCGAACTTATATCAAAAGCCACTACCTTT
AGAACCTGATAGTGAAAGAATTCAACAAGATTGGAAAATACTAAAAGAAGCAAAACACCCCTTGATATATATCGGACAAG
GAACAAGAGGAGCACGTGAAGAAGTTTTAGCTCTTTCTAAAATGCTACAAGCTCCAATTTTGAGCTCAGCTCTAGCTAAA
GGGATAGTTCCCGATAATGCGCCTGCATATATGGGCTCTGCTGGACGGGTTGCTTCTAAACCGGGGGTAGAGATTGCCTC
TAATACTGATGCTGTGCTATTCTTAGGTTCAGATATGCATTTCCAAAGTTTCTTTATCCCTGAAGATGCCAAATATATTC
AAGTTGACATTGATGGAACTAAATTTGGACGTCGTCATCAAGTTGATTTGGCTATTTTGGCTGACGCTAAATTGACCATA
AAAAAATTAATGGAAATTGGAGAACAACTTCCAGAAACGGCTTGGTATCGTGCAGCCTTAGCAGACAAAGAAAATTGGGT
AGAATGGCTTGAAAAACGTTCGCTTATTGATAGTTCGCCATTAGAAGTAGAGCCCGTCTTTAAAGCAATCAATGAAATGG
CAACTGATGATGCTATTTTCCAAGTTGATGTGGGAAATGTAACAATCGATGGCATGCGACTTCTTGATACAAGTAAAAAC
CAAAGATTTACGACAAGTGGTTGGTATGCTACGATGGGCTATGCTTTGCCGGCAGCAATTGGGGCCAAGTCTCAATATCC
AAACCGACAAGTTTGGTCAATTTCTGGGGATGGAGCTTGGGCGATGGTGATGCCTGATATTTTAACTCAGGTCAAATACC
ATTTACCAATCATTAATGTTGTTTTAACTAATAAGAGCCTAGGCTTTATTGAAGCAGAACAAGATGATGCCAAACAACCA
CACTCTGGTATTGATTTGATTGGAGCAAATTTTGGAGCTTCGGCAGCAGCTTTGGGTGCAGTAGGGTTTGATGTCAATAA
CTTGTCAGAACTGAAAGAGGCCTTTGCAAAAGCGAAAGAAACTGATCGACCAGTTGTCATTAATGTCAATATTTTTGACC
ATCGTCCCTTGCCTGTTGAACAATTAAAACTTGATTCATCGATTTACGGAGAAGAAGCTGTTGAGGCTTTCGAGAAAGAA
TATCAAACCCAAGGTTTAATTCCTTTCAGTAAACTTATTAAAGAATAA

Upstream 100 bases:

>100_bases
ATTTACCTGAAAATGAAAGGGGAATGTTTAATAATTAGTATTTTTTAAAGCAGTGTTAACATAGTTAGTAATTGATAGAT
TGAACATCAAGGAGAAAATC

Downstream 100 bases:

>100_bases
AAAAATTATTAGAAAAAAAGATACTGGATATTCCAGTGTATTTTTTTTATTTTGAAAACCTTAAACAAAATGAAGGGGAA
TACAAAACGACATTTTAAAA

Product: pyruvate oxidase

Products: NA

Alternate protein names: Pyruvic oxidase; POX [H]

Number of amino acids: Translated: 575; Mature: 574

Protein sequence:

>575_residues
MTKINAADAMLKVIEDWGVKNIYGLPGGSIDSTMNALYNRKDTLNYIQVRHEEVGALAAVGEAKVTGEIGVTFGSAGPGA
VHLLNGLYDAQYDHVPVLALVGQVPTSAMNTNYFQELNENPMFADVSVYNRTVMTAEQMPAVVDEAIRQAYAKRGVAVIT
IPKDLGWQEIEDNYISSANLYQKPLPLEPDSERIQQDWKILKEAKHPLIYIGQGTRGAREEVLALSKMLQAPILSSALAK
GIVPDNAPAYMGSAGRVASKPGVEIASNTDAVLFLGSDMHFQSFFIPEDAKYIQVDIDGTKFGRRHQVDLAILADAKLTI
KKLMEIGEQLPETAWYRAALADKENWVEWLEKRSLIDSSPLEVEPVFKAINEMATDDAIFQVDVGNVTIDGMRLLDTSKN
QRFTTSGWYATMGYALPAAIGAKSQYPNRQVWSISGDGAWAMVMPDILTQVKYHLPIINVVLTNKSLGFIEAEQDDAKQP
HSGIDLIGANFGASAAALGAVGFDVNNLSELKEAFAKAKETDRPVVINVNIFDHRPLPVEQLKLDSSIYGEEAVEAFEKE
YQTQGLIPFSKLIKE

Sequences:

>Translated_575_residues
MTKINAADAMLKVIEDWGVKNIYGLPGGSIDSTMNALYNRKDTLNYIQVRHEEVGALAAVGEAKVTGEIGVTFGSAGPGA
VHLLNGLYDAQYDHVPVLALVGQVPTSAMNTNYFQELNENPMFADVSVYNRTVMTAEQMPAVVDEAIRQAYAKRGVAVIT
IPKDLGWQEIEDNYISSANLYQKPLPLEPDSERIQQDWKILKEAKHPLIYIGQGTRGAREEVLALSKMLQAPILSSALAK
GIVPDNAPAYMGSAGRVASKPGVEIASNTDAVLFLGSDMHFQSFFIPEDAKYIQVDIDGTKFGRRHQVDLAILADAKLTI
KKLMEIGEQLPETAWYRAALADKENWVEWLEKRSLIDSSPLEVEPVFKAINEMATDDAIFQVDVGNVTIDGMRLLDTSKN
QRFTTSGWYATMGYALPAAIGAKSQYPNRQVWSISGDGAWAMVMPDILTQVKYHLPIINVVLTNKSLGFIEAEQDDAKQP
HSGIDLIGANFGASAAALGAVGFDVNNLSELKEAFAKAKETDRPVVINVNIFDHRPLPVEQLKLDSSIYGEEAVEAFEKE
YQTQGLIPFSKLIKE
>Mature_574_residues
TKINAADAMLKVIEDWGVKNIYGLPGGSIDSTMNALYNRKDTLNYIQVRHEEVGALAAVGEAKVTGEIGVTFGSAGPGAV
HLLNGLYDAQYDHVPVLALVGQVPTSAMNTNYFQELNENPMFADVSVYNRTVMTAEQMPAVVDEAIRQAYAKRGVAVITI
PKDLGWQEIEDNYISSANLYQKPLPLEPDSERIQQDWKILKEAKHPLIYIGQGTRGAREEVLALSKMLQAPILSSALAKG
IVPDNAPAYMGSAGRVASKPGVEIASNTDAVLFLGSDMHFQSFFIPEDAKYIQVDIDGTKFGRRHQVDLAILADAKLTIK
KLMEIGEQLPETAWYRAALADKENWVEWLEKRSLIDSSPLEVEPVFKAINEMATDDAIFQVDVGNVTIDGMRLLDTSKNQ
RFTTSGWYATMGYALPAAIGAKSQYPNRQVWSISGDGAWAMVMPDILTQVKYHLPIINVVLTNKSLGFIEAEQDDAKQPH
SGIDLIGANFGASAAALGAVGFDVNNLSELKEAFAKAKETDRPVVINVNIFDHRPLPVEQLKLDSSIYGEEAVEAFEKEY
QTQGLIPFSKLIKE

Specific function: Important for the aerobic growth. Decarboxylates pyruvate in four steps. The energy released is partially stored in acetyl phosphate [H]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=559, Percent_Identity=24.865831842576, Blast_Score=152, Evalue=1e-36,
Organism=Homo sapiens, GI21361361, Length=567, Percent_Identity=23.4567901234568, Blast_Score=114, Evalue=3e-25,
Organism=Escherichia coli, GI1787096, Length=546, Percent_Identity=32.4175824175824, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI1790104, Length=545, Percent_Identity=27.7064220183486, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI87081685, Length=555, Percent_Identity=26.6666666666667, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1786717, Length=559, Percent_Identity=25.9391771019678, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1788716, Length=549, Percent_Identity=22.7686703096539, Blast_Score=106, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17531299, Length=560, Percent_Identity=24.8214285714286, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17531301, Length=560, Percent_Identity=24.8214285714286, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17542570, Length=585, Percent_Identity=22.2222222222222, Blast_Score=114, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6323755, Length=563, Percent_Identity=24.1563055062167, Blast_Score=144, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6320816, Length=559, Percent_Identity=25.7602862254025, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6320123, Length=505, Percent_Identity=22.1782178217822, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI19922626, Length=560, Percent_Identity=25.7142857142857, Blast_Score=164, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014092
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =1.2.3.3 [H]

Molecular weight: Translated: 63081; Mature: 62950

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKINAADAMLKVIEDWGVKNIYGLPGGSIDSTMNALYNRKDTLNYIQVRHEEVGALAAV
CCCCCHHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC
GEAKVTGEIGVTFGSAGPGAVHLLNGLYDAQYDHVPVLALVGQVPTSAMNTNYFQELNEN
CCCEEECEEEEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHCCHHHHHCCCC
PMFADVSVYNRTVMTAEQMPAVVDEAIRQAYAKRGVAVITIPKDLGWQEIEDNYISSANL
CCEEEHHHHCCEEEEHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHCCCCHH
YQKPLPLEPDSERIQQDWKILKEAKHPLIYIGQGTRGAREEVLALSKMLQAPILSSALAK
CCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
GIVPDNAPAYMGSAGRVASKPGVEIASNTDAVLFLGSDMHFQSFFIPEDAKYIQVDIDGT
CCCCCCCCCCCCCCCCCCCCCCCEEECCCCEEEEEECCCCEEEEECCCCCEEEEEEECCC
KFGRRHQVDLAILADAKLTIKKLMEIGEQLPETAWYRAALADKENWVEWLEKRSLIDSSP
CCCCCEEEEEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCC
LEVEPVFKAINEMATDDAIFQVDVGNVTIDGMRLLDTSKNQRFTTSGWYATMGYALPAAI
CCHHHHHHHHHHHCCCCCEEEEECCCEEECCEEEEECCCCCEEECCCCHHHHHHHHHHHH
GAKSQYPNRQVWSISGDGAWAMVMPDILTQVKYHLPIINVVLTNKSLGFIEAEQDDAKQP
CCCCCCCCCEEEEECCCCEEEEHHHHHHHHHHHCCCEEEEEEECCCCCEEECCCCCCCCC
HSGIDLIGANFGASAAALGAVGFDVNNLSELKEAFAKAKETDRPVVINVNIFDHRPLPVE
CCCCEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHH
QLKLDSSIYGEEAVEAFEKEYQTQGLIPFSKLIKE
HHCCCCHHCCHHHHHHHHHHHHHCCCCCHHHHHCC
>Mature Secondary Structure 
TKINAADAMLKVIEDWGVKNIYGLPGGSIDSTMNALYNRKDTLNYIQVRHEEVGALAAV
CCCCHHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC
GEAKVTGEIGVTFGSAGPGAVHLLNGLYDAQYDHVPVLALVGQVPTSAMNTNYFQELNEN
CCCEEECEEEEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHCCHHHHHCCCC
PMFADVSVYNRTVMTAEQMPAVVDEAIRQAYAKRGVAVITIPKDLGWQEIEDNYISSANL
CCEEEHHHHCCEEEEHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHCCCCHH
YQKPLPLEPDSERIQQDWKILKEAKHPLIYIGQGTRGAREEVLALSKMLQAPILSSALAK
CCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
GIVPDNAPAYMGSAGRVASKPGVEIASNTDAVLFLGSDMHFQSFFIPEDAKYIQVDIDGT
CCCCCCCCCCCCCCCCCCCCCCCEEECCCCEEEEEECCCCEEEEECCCCCEEEEEEECCC
KFGRRHQVDLAILADAKLTIKKLMEIGEQLPETAWYRAALADKENWVEWLEKRSLIDSSP
CCCCCEEEEEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCC
LEVEPVFKAINEMATDDAIFQVDVGNVTIDGMRLLDTSKNQRFTTSGWYATMGYALPAAI
CCHHHHHHHHHHHCCCCCEEEEECCCEEECCEEEEECCCCCEEECCCCHHHHHHHHHHHH
GAKSQYPNRQVWSISGDGAWAMVMPDILTQVKYHLPIINVVLTNKSLGFIEAEQDDAKQP
CCCCCCCCCEEEEECCCCEEEEHHHHHHHHHHHCCCEEEEEEECCCCCEEECCCCCCCCC
HSGIDLIGANFGASAAALGAVGFDVNNLSELKEAFAKAKETDRPVVINVNIFDHRPLPVE
CCCCEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHH
QLKLDSSIYGEEAVEAFEKEYQTQGLIPFSKLIKE
HHCCCCHHCCHHHHHHHHHHHHHCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12566566; 8145244; 8438155 [H]