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The map label for this gene is rrmA [H]

Identifier: 125625011

GI number: 125625011

Start: 2208602

End: 2209423

Strand: Reverse

Name: rrmA [H]

Synonym: llmg_2246

Alternate gene names: 125625011

Gene position: 2209423-2208602 (Counterclockwise)

Preceding gene: 125625012

Following gene: 125625009

Centisome position: 87.35

GC content: 34.55

Gene sequence:

>822_bases
ATGTTAAAAAAAATCGAAAAAGCTTATTTATTTTTAGAGAAGAATTTAGAAGTGCTAAGATGCCCAATTTGTCAGGGAAA
ATTTCAATTGGAAGCCAATGCCTTGAAATGTCAAAATAATCATACCTATAATTTAAATAAGAAAGGTTATGTGAATTTTC
TACAAACCAAAGCTGATACAGAACATTACACGCGAAAAATGTTTGAGCCTCGCAGACGTCTGATTCAAGCTGGGATGTAT
CAACATTTACTGACAGAAATTCAAAAATATTTTGTCAGTGGAAATTTGCTTGATGTTGGGACTGGGGAAGGTTCATTTTT
GGAGCTCCTTAAGGTAGATGGAGCAAAATTTGCTTTTGATATTGCTAAAGATGGGATTGAAATGGCGACTGAACTAGAGA
TGGAAAGTTTTTTAAGTTTAGCTGACTTGACTAATCTCCCTTTTGCTGATGAAAGCCTGTCAGTAATTTTGAATATCTTC
ACTCCGTCAAATTATGCAGAATTTCACCGTGTATTAGCAGAAAGTGGTTTAGTCATCAAAATTATTCCTGATAAAAATTA
TCTGCGAGAATTGCGTGAAGTTTATCAACTCCCCGTTGATTATAATAATCAGCCAGTTCTTGAACGTTTTAGAGAAGAAT
TTCCTAATGCCAGTCAAGAAGAAATTGAATACCAATTCGAGATTCCGAAAAATTTGCGTCATGATTTTTTACTGATGAGC
CCTTTGGAATGGTCTGTTTCTGATGAAAGAAAAAAATTTGCTAAAGAAAACCCACCAAAAACGGCCACAATTCATGTCCA
GATTCTAGTGGGGAGAAAATAA

Upstream 100 bases:

>100_bases
ATATTGTGGTCTAATGGGTCGTTTTTTATTGCATAATTGTATAAAACGCGTATTTTACCCTCTATTTGGCTCTAATGGGG
AGCTGTGATAAAATAGAACT

Downstream 100 bases:

>100_bases
AAAAGCTGTCAATCAGACAGCTTTTATTTTTATTTCATTAATAATTTAATTGCTTTAGCAATTCCTTTTTTTACAGAAAT
ATGAAGAACAAGCGAAAGAA

Product: putative rRNA (guanine-N1-)-methyltransferase

Products: S-adenosyl-L-homocysteine +rRNA containing Nmethylguanine [C]

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MLKKIEKAYLFLEKNLEVLRCPICQGKFQLEANALKCQNNHTYNLNKKGYVNFLQTKADTEHYTRKMFEPRRRLIQAGMY
QHLLTEIQKYFVSGNLLDVGTGEGSFLELLKVDGAKFAFDIAKDGIEMATELEMESFLSLADLTNLPFADESLSVILNIF
TPSNYAEFHRVLAESGLVIKIIPDKNYLRELREVYQLPVDYNNQPVLERFREEFPNASQEEIEYQFEIPKNLRHDFLLMS
PLEWSVSDERKKFAKENPPKTATIHVQILVGRK

Sequences:

>Translated_273_residues
MLKKIEKAYLFLEKNLEVLRCPICQGKFQLEANALKCQNNHTYNLNKKGYVNFLQTKADTEHYTRKMFEPRRRLIQAGMY
QHLLTEIQKYFVSGNLLDVGTGEGSFLELLKVDGAKFAFDIAKDGIEMATELEMESFLSLADLTNLPFADESLSVILNIF
TPSNYAEFHRVLAESGLVIKIIPDKNYLRELREVYQLPVDYNNQPVLERFREEFPNASQEEIEYQFEIPKNLRHDFLLMS
PLEWSVSDERKKFAKENPPKTATIHVQILVGRK
>Mature_273_residues
MLKKIEKAYLFLEKNLEVLRCPICQGKFQLEANALKCQNNHTYNLNKKGYVNFLQTKADTEHYTRKMFEPRRRLIQAGMY
QHLLTEIQKYFVSGNLLDVGTGEGSFLELLKVDGAKFAFDIAKDGIEMATELEMESFLSLADLTNLPFADESLSVILNIF
TPSNYAEFHRVLAESGLVIKIIPDKNYLRELREVYQLPVDYNNQPVLERFREEFPNASQEEIEYQFEIPKNLRHDFLLMS
PLEWSVSDERKKFAKENPPKTATIHVQILVGRK

Specific function: Specifically Methylates The Guanosine In Position 745 Of 23s rRNA. [C]

COG id: COG0500

COG function: function code QR; SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmA family [H]

Homologues:

Organism=Escherichia coli, GI1788125, Length=246, Percent_Identity=28.4552845528455, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013216
- InterPro:   IPR016718 [H]

Pfam domain/function: PF08241 Methyltransf_11 [H]

EC number: 2.1.1.51 [C]

Molecular weight: Translated: 31763; Mature: 31763

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKIEKAYLFLEKNLEVLRCPICQGKFQLEANALKCQNNHTYNLNKKGYVNFLQTKADT
CCCHHHHHHHHHHCCCCEEECCCCCCCEEEECCEEEECCCCEEECCCCCCHHHHHHCCCH
EHYTRKMFEPRRRLIQAGMYQHLLTEIQKYFVSGNLLDVGTGEGSFLELLKVDGAKFAFD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHEEEEECCCCCEEHH
IAKDGIEMATELEMESFLSLADLTNLPFADESLSVILNIFTPSNYAEFHRVLAESGLVIK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCHHHHHHHHHCCCCEEE
IIPDKNYLRELREVYQLPVDYNNQPVLERFREEFPNASQEEIEYQFEIPKNLRHDFLLMS
EECCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHEEEEEECCCCCCCCEEEEC
PLEWSVSDERKKFAKENPPKTATIHVQILVGRK
CCCCCCCHHHHHHHHCCCCCEEEEEEEEEECCC
>Mature Secondary Structure
MLKKIEKAYLFLEKNLEVLRCPICQGKFQLEANALKCQNNHTYNLNKKGYVNFLQTKADT
CCCHHHHHHHHHHCCCCEEECCCCCCCEEEECCEEEECCCCEEECCCCCCHHHHHHCCCH
EHYTRKMFEPRRRLIQAGMYQHLLTEIQKYFVSGNLLDVGTGEGSFLELLKVDGAKFAFD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHEEEEECCCCCEEHH
IAKDGIEMATELEMESFLSLADLTNLPFADESLSVILNIFTPSNYAEFHRVLAESGLVIK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCHHHHHHHHHCCCCEEE
IIPDKNYLRELREVYQLPVDYNNQPVLERFREEFPNASQEEIEYQFEIPKNLRHDFLLMS
EECCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHEEEEEECCCCCCCCEEEEC
PLEWSVSDERKKFAKENPPKTATIHVQILVGRK
CCCCCCCHHHHHHHHCCCCCEEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Unidentified protein factor B [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; rRNA [C]

Specific reaction: S-Adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine +rRNA containing Nmethylguanine [C]

General reaction: Methyl group transfer [C]

Inhibitor: Mg2+; Unidentified protein factor A1 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969509; 9384377 [H]