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The map label for this gene is hisS [H]

Identifier: 125624984

GI number: 125624984

Start: 2180691

End: 2181983

Strand: Reverse

Name: hisS [H]

Synonym: llmg_2217

Alternate gene names: 125624984

Gene position: 2181983-2180691 (Counterclockwise)

Preceding gene: 125624985

Following gene: 125624983

Centisome position: 86.26

GC content: 37.74

Gene sequence:

>1293_bases
ATGAAACTTCAAAAACCAAAAGGAACAGCGGATTTATTACCTGCTGAAACTGCAAAATGGCAGTATATCGAAGAAATTGC
TCGTGGCGTTTTTAATGATTATAATTTCAAAGAGATTCGTACCCCAATGTTTGAAAGCTATGAACTCTTTAGTCGTGCCA
CTGGTGAAACCAGTGATATTGTTACTAAAGAAATGTATGATTTCGAGGATAAAGGTGGACGTCATATTGCCCTTCGTCCA
GAGGGAACAGCATCAGCCGTTCGTGCTTACATCGAAAATAAACTCTATGCTCCAGAAGTTGTGAAACCAGTAAAACTTTG
GTATGATGCTCCAATGTTTCGTTATGAACGTCCACAATCAGGACGTTTACGTCAATTCCACCAATTTGGTGTAGAATGCT
TAGGCTTGAAAAATCCAGCGGTCGATGTAGAAATTATTGCGATGGCTGATACGCTTTTCCGTCAATTAGGCATTTCTGGG
GTGAAGCTTTCGCTTAACACATTAGGGGATATGGAAAGCCGTAAGGCATATCGACAAGCTTTGATTGATTATTTGACTCC
GTTTGAAAATCAACTTTCAGAAGATAGCCGTCGTCGTTTAAATGAAAATCCTTTACGAGTGTTGGATAGTAAAGAAGCAG
AAGATATCGCAATTGTGAAAAATGCCCCTGCTATCTTGGACTATCTCAATGAAACTTCAAAAGCTTATTTTGAAGAAGTA
AAAGCCTTACTCGAAGCTTTGAACATCGAATATACCATTGACTCAAATATGGTTCGTGGTTTAGATTACTATAATGATAC
AATTTTTGAATTCATTGTTAATTTTGATGGTAAAGAACTAACTGTTTGTGGTGGTGGACGTTATGACGGTCTAGTAGAGT
ATTTTGATGGTCCAGCCACTCCAGCCTTCGGTTTTGGTTTAGGTATCGAAAGACTCCTAATGATTGCCGAAAAACAAGAA
ATTAACTTTATTCCTGAGGAAACTTTGGATGTTTACATTGCTGTAATGGGTGAAAAAGCTAATCTTGAAGCAACAAAATT
GGCTGAGAGTTTACGCGAACAAGCCTTCAAAGTAGAACGTGATTTTTCAAATCGTAAACTTGGAGCACAATTTAAAACTG
CCGAAAAATTGGGAGCAGAATTGATTATTACACTTGGAGAAGATGAAGTTCGCACAGGACAAATCAAAGTGAAACATAAT
CAAACACGTAAACAAGTAGAAACAACACTCCAAGCGGTTCACGAATCATTTGCCCCAATTTTTGAAGAAATTTATGCTGA
CGAGATAAACTAA

Upstream 100 bases:

>100_bases
TTGGGGTGCTTTCTTATGTAAACGGTATCTTATTAGATAAGTATCACATATAATGATTTTTTACAAAGACTCCCACTCCC
AGTGAATCGAAAGGAAAAAA

Downstream 100 bases:

>100_bases
GTCAAGAAAAGGATCAGGTGAAGAGATGAAAGATTGTCCATTTTGTGAGGTGAAAGATAAGGTCTTTGAAAATGAATTGG
CACAGGCATTTTATGATGCT

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MKLQKPKGTADLLPAETAKWQYIEEIARGVFNDYNFKEIRTPMFESYELFSRATGETSDIVTKEMYDFEDKGGRHIALRP
EGTASAVRAYIENKLYAPEVVKPVKLWYDAPMFRYERPQSGRLRQFHQFGVECLGLKNPAVDVEIIAMADTLFRQLGISG
VKLSLNTLGDMESRKAYRQALIDYLTPFENQLSEDSRRRLNENPLRVLDSKEAEDIAIVKNAPAILDYLNETSKAYFEEV
KALLEALNIEYTIDSNMVRGLDYYNDTIFEFIVNFDGKELTVCGGGRYDGLVEYFDGPATPAFGFGLGIERLLMIAEKQE
INFIPEETLDVYIAVMGEKANLEATKLAESLREQAFKVERDFSNRKLGAQFKTAEKLGAELIITLGEDEVRTGQIKVKHN
QTRKQVETTLQAVHESFAPIFEEIYADEIN

Sequences:

>Translated_430_residues
MKLQKPKGTADLLPAETAKWQYIEEIARGVFNDYNFKEIRTPMFESYELFSRATGETSDIVTKEMYDFEDKGGRHIALRP
EGTASAVRAYIENKLYAPEVVKPVKLWYDAPMFRYERPQSGRLRQFHQFGVECLGLKNPAVDVEIIAMADTLFRQLGISG
VKLSLNTLGDMESRKAYRQALIDYLTPFENQLSEDSRRRLNENPLRVLDSKEAEDIAIVKNAPAILDYLNETSKAYFEEV
KALLEALNIEYTIDSNMVRGLDYYNDTIFEFIVNFDGKELTVCGGGRYDGLVEYFDGPATPAFGFGLGIERLLMIAEKQE
INFIPEETLDVYIAVMGEKANLEATKLAESLREQAFKVERDFSNRKLGAQFKTAEKLGAELIITLGEDEVRTGQIKVKHN
QTRKQVETTLQAVHESFAPIFEEIYADEIN
>Mature_430_residues
MKLQKPKGTADLLPAETAKWQYIEEIARGVFNDYNFKEIRTPMFESYELFSRATGETSDIVTKEMYDFEDKGGRHIALRP
EGTASAVRAYIENKLYAPEVVKPVKLWYDAPMFRYERPQSGRLRQFHQFGVECLGLKNPAVDVEIIAMADTLFRQLGISG
VKLSLNTLGDMESRKAYRQALIDYLTPFENQLSEDSRRRLNENPLRVLDSKEAEDIAIVKNAPAILDYLNETSKAYFEEV
KALLEALNIEYTIDSNMVRGLDYYNDTIFEFIVNFDGKELTVCGGGRYDGLVEYFDGPATPAFGFGLGIERLLMIAEKQE
INFIPEETLDVYIAVMGEKANLEATKLAESLREQAFKVERDFSNRKLGAQFKTAEKLGAELIITLGEDEVRTGQIKVKHN
QTRKQVETTLQAVHESFAPIFEEIYADEIN

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI15029520, Length=455, Percent_Identity=26.5934065934066, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI6996014, Length=461, Percent_Identity=26.0303687635575, Blast_Score=109, Evalue=5e-24,
Organism=Escherichia coli, GI1788861, Length=415, Percent_Identity=45.0602409638554, Blast_Score=362, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI71993693, Length=470, Percent_Identity=27.2340425531915, Blast_Score=124, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI71993686, Length=470, Percent_Identity=27.2340425531915, Blast_Score=124, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6325290, Length=473, Percent_Identity=24.3128964059197, Blast_Score=100, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24643061, Length=463, Percent_Identity=23.9740820734341, Blast_Score=111, Evalue=9e-25,
Organism=Drosophila melanogaster, GI24643059, Length=482, Percent_Identity=23.0290456431535, Blast_Score=101, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 48985; Mature: 48985

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQKPKGTADLLPAETAKWQYIEEIARGVFNDYNFKEIRTPMFESYELFSRATGETSDI
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHCCCCCHHH
VTKEMYDFEDKGGRHIALRPEGTASAVRAYIENKLYAPEVVKPVKLWYDAPMFRYERPQS
HHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHCCCCCCC
GRLRQFHQFGVECLGLKNPAVDVEIIAMADTLFRQLGISGVKLSLNTLGDMESRKAYRQA
CHHHHHHHCCCEEEECCCCCCCEEEEHHHHHHHHHHCCCCEEEEHHHHCCHHHHHHHHHH
LIDYLTPFENQLSEDSRRRLNENPLRVLDSKEAEDIAIVKNAPAILDYLNETSKAYFEEV
HHHHHCCHHHHHCHHHHHHCCCCCHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHH
KALLEALNIEYTIDSNMVRGLDYYNDTIFEFIVNFDGKELTVCGGGRYDGLVEYFDGPAT
HHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHCCCCCC
PAFGFGLGIERLLMIAEKQEINFIPEETLDVYIAVMGEKANLEATKLAESLREQAFKVER
CCHHHHHHHHHHHHHHHHHCCCCCCCHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
DFSNRKLGAQFKTAEKLGAELIITLGEDEVRTGQIKVKHNQTRKQVETTLQAVHESFAPI
CCCCCCCCCHHHHHHHCCCEEEEEECCCCCEECEEEEECCHHHHHHHHHHHHHHHHHHHH
FEEIYADEIN
HHHHHHCCCC
>Mature Secondary Structure
MKLQKPKGTADLLPAETAKWQYIEEIARGVFNDYNFKEIRTPMFESYELFSRATGETSDI
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHCCCCCHHH
VTKEMYDFEDKGGRHIALRPEGTASAVRAYIENKLYAPEVVKPVKLWYDAPMFRYERPQS
HHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHCCCCCCC
GRLRQFHQFGVECLGLKNPAVDVEIIAMADTLFRQLGISGVKLSLNTLGDMESRKAYRQA
CHHHHHHHCCCEEEECCCCCCCEEEEHHHHHHHHHHCCCCEEEEHHHHCCHHHHHHHHHH
LIDYLTPFENQLSEDSRRRLNENPLRVLDSKEAEDIAIVKNAPAILDYLNETSKAYFEEV
HHHHHCCHHHHHCHHHHHHCCCCCHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHH
KALLEALNIEYTIDSNMVRGLDYYNDTIFEFIVNFDGKELTVCGGGRYDGLVEYFDGPAT
HHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHCCCCCC
PAFGFGLGIERLLMIAEKQEINFIPEETLDVYIAVMGEKANLEATKLAESLREQAFKVER
CCHHHHHHHHHHHHHHHHHCCCCCCCHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
DFSNRKLGAQFKTAEKLGAELIITLGEDEVRTGQIKVKHNQTRKQVETTLQAVHESFAPI
CCCCCCCCCHHHHHHHCCCEEEEEECCCCCEECEEEEECCHHHHHHHHHHHHHHHHHHHH
FEEIYADEIN
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]