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The map label for this gene is aspS [H]

Identifier: 125624982

GI number: 125624982

Start: 2178461

End: 2180233

Strand: Reverse

Name: aspS [H]

Synonym: llmg_2215

Alternate gene names: 125624982

Gene position: 2180233-2178461 (Counterclockwise)

Preceding gene: 125624983

Following gene: 125624981

Centisome position: 86.19

GC content: 38.41

Gene sequence:

>1773_bases
ATGAAACGTACAAATTACGCGGGAAATATTACAGAAGAATATTTAAATCAAACAATCACAGTTAAAGGTTGGGTGGCTAA
ACGCCGTAACCTTGGAGGATTGATTTTCATTGATTTACGTGACCGAGAAGGAATTGTCCAAATTGTGGTCAATCCTGAAA
CAGCAGCAACCGAAGTGGCTGAAGCCGCGGATAAAGCACGTAATGAATTTGTCCTTGAAGTAACGGGTAAAGTGGTTGAG
CGTGCAAGTAAGAATGACAAAATCAAAACCGGTGGTATTGAAATCGAAGCGACTGCTATCGAAATTTTGTCTACTTCTAA
AACAACACCTTTTGAAATCAAAGATGATGTTGAAGTACTTGATGATACACGTTTGAAATATCGTTACCTTGATTTACGTC
GTCCAGAAATGCTTAAAAATATCACAATGCGTCATGCGACGACTCGTTCAATTCGTGAATATTTGGATGGAGCAGGCTTT
ATTGATGTTGAAACGCCATTTTTGAACAAATCAACACCAGAAGGAGCGCGTGATTATCTTGTCCCTTCTCGAGTTAATAA
AGGCGAATTTTACGCTTTACCGCAATCACCACAATTGATGAAACAACTTTTGATGACTGCTGGTCTTGATCGTTACTATC
AAATTGTGAAATGTTTCCGTGATGAAGATTTACGTGGTGACCGTCAGCCAGAATTCACACAAGTCGATTTAGAAACTTCA
TTTTTGGGTGAAGAAGAAATTCAAGAGTTGACTGAAGAATTGATTGCTAAAGTCATGAAAGATGTCAAAGGAATTGATGT
GACTTTACCATTCCCAAGAATGAACTATGATGATGCCATGAATTTTTATGGTTCTGACAAACCTGACACACGTTTTGAAT
TGTTACTGACAGACTTGTCAGCACTTGCTAAAACAGTTGATTTCAAAGTTTTCCAAGAAGCTGAAGTTGTTAAAGCAATT
GTTGTCAAAGGCGCTGCTGACAAATATTCTCGTAAATCAATTGATAAACTGACAGAACAAGCCAAACAAAATGGAGCTAA
AGGTCTTGCCTGGGTCAAATTTGAAAAAGGGGAATTCGCCGGTGGTGTTTCAAAATTCTTAGCTGAATCTACTGACAGCT
TTGTTAATGAATTAAAATTGACTGACAATGACTTGGTACTTTTTGTTGCTGACAGCCTTGATGTAGCGAATTCTGCTCTT
GGTGCCCTTCGTTTAACAATTGGTAAACAACAAGGTCTGATTGATTTCCGTAAGTTCAATTTCCTTTGGGTCATTGATTG
GCCAATGTTTGAATGGTCAGATGAAGAAGAACGTTACATGTCTGCTCACCATCCATTTACTTTACCAACGAAAGAAACAC
AAGCTTTCTTATCTGCTGACGGACATCGGAAAGATTCTGATTTGAAAAAAGTTCGTGCGCATGCTTATGATATTGTCCTT
AATGGTTATGAACTTGGCGGAGGTTCTTTGAGAATTAATAGTCGTGACCTTCAAGAAGAAATGCTGAGTGCTCTTGGCTT
TAAACTAGAAGATGCCAATGAACAATTTGGCTTCTTACTTGAAGCACTTGACTATGGTTTCCCACCACATGGTGGATTGG
CTCTTGGTCTTGACCGTTTTGTAATGTTACTTGCTGGTAAAGATAATATCCGTGAAGTAATTGCGTTCCCTAAAAACAAT
AAAGCCTCTGATCCAATGACTCAGGCTCCATCTATAGTAGCTGACAAACAGCTGGAAGAATTGAGTATTAAACTTGCAAA
CAAAGATCAATAA

Upstream 100 bases:

>100_bases
TCGTGGCGTGATTCCTGAAAAACAAAATTATCGATAATAAGAAGAAAGAGGTCTTAAATTTTACCTGTTTGTACTAGAGA
ACAGTGAAATTTACATTTAT

Downstream 100 bases:

>100_bases
AATTGTCGGTGGCCTTTTAGATAAAATTGGACTTTACCAAGTATTAAAATCTATTGGCGCTGAAAAAATGGCAAATAAAT
TTTCGGCAGCAGTTGTATAT

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 590; Mature: 590

Protein sequence:

>590_residues
MKRTNYAGNITEEYLNQTITVKGWVAKRRNLGGLIFIDLRDREGIVQIVVNPETAATEVAEAADKARNEFVLEVTGKVVE
RASKNDKIKTGGIEIEATAIEILSTSKTTPFEIKDDVEVLDDTRLKYRYLDLRRPEMLKNITMRHATTRSIREYLDGAGF
IDVETPFLNKSTPEGARDYLVPSRVNKGEFYALPQSPQLMKQLLMTAGLDRYYQIVKCFRDEDLRGDRQPEFTQVDLETS
FLGEEEIQELTEELIAKVMKDVKGIDVTLPFPRMNYDDAMNFYGSDKPDTRFELLLTDLSALAKTVDFKVFQEAEVVKAI
VVKGAADKYSRKSIDKLTEQAKQNGAKGLAWVKFEKGEFAGGVSKFLAESTDSFVNELKLTDNDLVLFVADSLDVANSAL
GALRLTIGKQQGLIDFRKFNFLWVIDWPMFEWSDEEERYMSAHHPFTLPTKETQAFLSADGHRKDSDLKKVRAHAYDIVL
NGYELGGGSLRINSRDLQEEMLSALGFKLEDANEQFGFLLEALDYGFPPHGGLALGLDRFVMLLAGKDNIREVIAFPKNN
KASDPMTQAPSIVADKQLEELSIKLANKDQ

Sequences:

>Translated_590_residues
MKRTNYAGNITEEYLNQTITVKGWVAKRRNLGGLIFIDLRDREGIVQIVVNPETAATEVAEAADKARNEFVLEVTGKVVE
RASKNDKIKTGGIEIEATAIEILSTSKTTPFEIKDDVEVLDDTRLKYRYLDLRRPEMLKNITMRHATTRSIREYLDGAGF
IDVETPFLNKSTPEGARDYLVPSRVNKGEFYALPQSPQLMKQLLMTAGLDRYYQIVKCFRDEDLRGDRQPEFTQVDLETS
FLGEEEIQELTEELIAKVMKDVKGIDVTLPFPRMNYDDAMNFYGSDKPDTRFELLLTDLSALAKTVDFKVFQEAEVVKAI
VVKGAADKYSRKSIDKLTEQAKQNGAKGLAWVKFEKGEFAGGVSKFLAESTDSFVNELKLTDNDLVLFVADSLDVANSAL
GALRLTIGKQQGLIDFRKFNFLWVIDWPMFEWSDEEERYMSAHHPFTLPTKETQAFLSADGHRKDSDLKKVRAHAYDIVL
NGYELGGGSLRINSRDLQEEMLSALGFKLEDANEQFGFLLEALDYGFPPHGGLALGLDRFVMLLAGKDNIREVIAFPKNN
KASDPMTQAPSIVADKQLEELSIKLANKDQ
>Mature_590_residues
MKRTNYAGNITEEYLNQTITVKGWVAKRRNLGGLIFIDLRDREGIVQIVVNPETAATEVAEAADKARNEFVLEVTGKVVE
RASKNDKIKTGGIEIEATAIEILSTSKTTPFEIKDDVEVLDDTRLKYRYLDLRRPEMLKNITMRHATTRSIREYLDGAGF
IDVETPFLNKSTPEGARDYLVPSRVNKGEFYALPQSPQLMKQLLMTAGLDRYYQIVKCFRDEDLRGDRQPEFTQVDLETS
FLGEEEIQELTEELIAKVMKDVKGIDVTLPFPRMNYDDAMNFYGSDKPDTRFELLLTDLSALAKTVDFKVFQEAEVVKAI
VVKGAADKYSRKSIDKLTEQAKQNGAKGLAWVKFEKGEFAGGVSKFLAESTDSFVNELKLTDNDLVLFVADSLDVANSAL
GALRLTIGKQQGLIDFRKFNFLWVIDWPMFEWSDEEERYMSAHHPFTLPTKETQAFLSADGHRKDSDLKKVRAHAYDIVL
NGYELGGGSLRINSRDLQEEMLSALGFKLEDANEQFGFLLEALDYGFPPHGGLALGLDRFVMLLAGKDNIREVIAFPKNN
KASDPMTQAPSIVADKQLEELSIKLANKDQ

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=600, Percent_Identity=39.8333333333333, Blast_Score=417, Evalue=1e-116,
Organism=Homo sapiens, GI194272210, Length=285, Percent_Identity=27.719298245614, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI5031815, Length=285, Percent_Identity=27.719298245614, Blast_Score=89, Evalue=2e-17,
Organism=Homo sapiens, GI45439306, Length=272, Percent_Identity=25, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI4758762, Length=292, Percent_Identity=27.0547945205479, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI1788173, Length=599, Percent_Identity=48.7479131886477, Blast_Score=536, Evalue=1e-153,
Organism=Escherichia coli, GI1789256, Length=297, Percent_Identity=28.6195286195286, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1790571, Length=293, Percent_Identity=26.2798634812287, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI32566633, Length=599, Percent_Identity=34.8914858096828, Blast_Score=327, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI71994340, Length=309, Percent_Identity=24.2718446601942, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17535925, Length=309, Percent_Identity=24.2718446601942, Blast_Score=96, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17535927, Length=309, Percent_Identity=24.2718446601942, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17551876, Length=299, Percent_Identity=24.4147157190635, Blast_Score=74, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71984122, Length=304, Percent_Identity=25, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6325153, Length=636, Percent_Identity=31.9182389937107, Blast_Score=267, Evalue=4e-72,
Organism=Saccharomyces cerevisiae, GI6321807, Length=287, Percent_Identity=25.4355400696864, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323011, Length=258, Percent_Identity=29.8449612403101, Blast_Score=67, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6320242, Length=145, Percent_Identity=29.6551724137931, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24584738, Length=608, Percent_Identity=37.6644736842105, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI17136276, Length=239, Percent_Identity=27.6150627615063, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24640849, Length=288, Percent_Identity=26.0416666666667, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24640851, Length=288, Percent_Identity=26.0416666666667, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI19921528, Length=280, Percent_Identity=27.8571428571429, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 66529; Mature: 66529

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRTNYAGNITEEYLNQTITVKGWVAKRRNLGGLIFIDLRDREGIVQIVVNPETAATEVA
CCCCCCCCCCHHHHCCCEEEEEEHHHHHCCCCCEEEEEEECCCCEEEEEECCCHHHHHHH
EAADKARNEFVLEVTGKVVERASKNDKIKTGGIEIEATAIEILSTSKTTPFEIKDDVEVL
HHHHHHHCCEEEEEHHHHHHHCCCCCCEEECCEEEEEEEEEEEECCCCCCEEECCCHHHH
DDTRLKYRYLDLRRPEMLKNITMRHATTRSIREYLDGAGFIDVETPFLNKSTPEGARDYL
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHCC
VPSRVNKGEFYALPQSPQLMKQLLMTAGLDRYYQIVKCFRDEDLRGDRQPEFTQVDLETS
CCCCCCCCCEEECCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCEEEECHHH
FLGEEEIQELTEELIAKVMKDVKGIDVTLPFPRMNYDDAMNFYGSDKPDTRFELLLTDLS
HCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHCCCCCCCCCHHHHHHHHHH
ALAKTVDFKVFQEAEVVKAIVVKGAADKYSRKSIDKLTEQAKQNGAKGLAWVKFEKGEFA
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
GGVSKFLAESTDSFVNELKLTDNDLVLFVADSLDVANSALGALRLTIGKQQGLIDFRKFN
CHHHHHHHHHHHHHHHHHEECCCCEEEEEECCCHHHHHHHHHEEEEECCCCCCEEEEECC
FLWVIDWPMFEWSDEEERYMSAHHPFTLPTKETQAFLSADGHRKDSDLKKVRAHAYDIVL
EEEEEECCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHEEEEE
NGYELGGGSLRINSRDLQEEMLSALGFKLEDANEQFGFLLEALDYGFPPHGGLALGLDRF
ECEEECCCEEEECHHHHHHHHHHHHCCEECCCCHHHHHHHHHHHCCCCCCCCCHHCHHHH
VMLLAGKDNIREVIAFPKNNKASDPMTQAPSIVADKQLEELSIKLANKDQ
HEECCCCCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHEEEECCCCC
>Mature Secondary Structure
MKRTNYAGNITEEYLNQTITVKGWVAKRRNLGGLIFIDLRDREGIVQIVVNPETAATEVA
CCCCCCCCCCHHHHCCCEEEEEEHHHHHCCCCCEEEEEEECCCCEEEEEECCCHHHHHHH
EAADKARNEFVLEVTGKVVERASKNDKIKTGGIEIEATAIEILSTSKTTPFEIKDDVEVL
HHHHHHHCCEEEEEHHHHHHHCCCCCCEEECCEEEEEEEEEEEECCCCCCEEECCCHHHH
DDTRLKYRYLDLRRPEMLKNITMRHATTRSIREYLDGAGFIDVETPFLNKSTPEGARDYL
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHCC
VPSRVNKGEFYALPQSPQLMKQLLMTAGLDRYYQIVKCFRDEDLRGDRQPEFTQVDLETS
CCCCCCCCCEEECCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCEEEECHHH
FLGEEEIQELTEELIAKVMKDVKGIDVTLPFPRMNYDDAMNFYGSDKPDTRFELLLTDLS
HCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHCCCCCCCCCHHHHHHHHHH
ALAKTVDFKVFQEAEVVKAIVVKGAADKYSRKSIDKLTEQAKQNGAKGLAWVKFEKGEFA
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
GGVSKFLAESTDSFVNELKLTDNDLVLFVADSLDVANSALGALRLTIGKQQGLIDFRKFN
CHHHHHHHHHHHHHHHHHEECCCCEEEEEECCCHHHHHHHHHEEEEECCCCCCEEEEECC
FLWVIDWPMFEWSDEEERYMSAHHPFTLPTKETQAFLSADGHRKDSDLKKVRAHAYDIVL
EEEEEECCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHEEEEE
NGYELGGGSLRINSRDLQEEMLSALGFKLEDANEQFGFLLEALDYGFPPHGGLALGLDRF
ECEEECCCEEEECHHHHHHHHHHHHCCEECCCCHHHHHHHHHHHCCCCCCCCCHHCHHHH
VMLLAGKDNIREVIAFPKNNKASDPMTQAPSIVADKQLEELSIKLANKDQ
HEECCCCCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]