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The map label for this gene is nifS [H]

Identifier: 125624820

GI number: 125624820

Start: 2032415

End: 2033296

Strand: Direct

Name: nifS [H]

Synonym: llmg_2048

Alternate gene names: 125624820

Gene position: 2032415-2033296 (Clockwise)

Preceding gene: 125624818

Following gene: 125624821

Centisome position: 80.35

GC content: 35.6

Gene sequence:

>882_bases
TTGGCAAATAGAGATAAAGGAAATCATATTATCACTACAGCTATTGAACATCCATCTGTTTTAAATACTGTTCAATACCT
TCATGACCGTTATAAATTTGATGTGACTTTTGTAAAACCAAATGCTGATGGCACTTTTCCAGCGGCACTCATTGAAAAAA
ACTTGCGTCCAGACACAATTTTAGTCAGCATGATGTGGGCTAATAATGAAACAGGACAACTGCTTCCAGTAAAGGAAGTC
GGAGAACTTTTGAGTCAACATCAAGCCGTTTTCCATGTTGATGCAACACAAGTTATGGGAAAAATTCCAGTTCACCCTCT
TGAAATTGGCGCCGATTTCTTATCTGCTTCTGCTCATAAATTTCATGGACCAAAAGGTGTCGGCTTCTTATACTATAATG
AACTTCTTCACTTTGATGCTCTGATTCATGGTGGTGAACAAGAAGAAAAAAGACGGGCTGGTACAGAAAATCTTCACTCA
CTGGTTGGTCTAGCTAAGGCCTTAGAACTCGCACTTGAAAATATTGAATCTAATTATCAAGCAGTCGAAGAACTCAATCA
CCACTTGCTCAGTAAATTAAAAGAACAAGAAATTCCTTTTTATAAAAATAATTTTGGTCCAAGTATGCCTCATGTTCTTA
ATCTTGCTTTTCCAAATGAGAATCATGATTTATTACTGACGAAACTGGATTTAGCTGGTTTTGCAATTTCAACTGGTTCT
GCTTGCACTGCTGGAACAATTGAACCTTCTCACGTTCTTGAGGCAGTTTACGGAAAAAATTCTGATAAACTCAAAGAAAA
TATTCGTATTTCATTTTCAGAATTAAACACACTTGATGAAGTTAATCTCTTTGTTGAAAAGCTTAGTTCTATTTTAAAAT
AA

Upstream 100 bases:

>100_bases
CCGTGAAACGGTCGCCACTGCTCTTGAAGTTCCAAGTCGAAATATCATTTTTACCACTGGCGCTAGCGAAGCCAATAATC
AAGCTTTAATTGGTTATGCC

Downstream 100 bases:

>100_bases
ATTAATATTTAGTCAGGAGAATGCAATGGCATTTATCACAGAAAAACATTTAGAGGGAAGCAAGTATGTTTATCAACTTG
CGCCTACCGTTAAAAAATTC

Product: putative iron-sulfur cofactor synthesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MANRDKGNHIITTAIEHPSVLNTVQYLHDRYKFDVTFVKPNADGTFPAALIEKNLRPDTILVSMMWANNETGQLLPVKEV
GELLSQHQAVFHVDATQVMGKIPVHPLEIGADFLSASAHKFHGPKGVGFLYYNELLHFDALIHGGEQEEKRRAGTENLHS
LVGLAKALELALENIESNYQAVEELNHHLLSKLKEQEIPFYKNNFGPSMPHVLNLAFPNENHDLLLTKLDLAGFAISTGS
ACTAGTIEPSHVLEAVYGKNSDKLKENIRISFSELNTLDEVNLFVEKLSSILK

Sequences:

>Translated_293_residues
MANRDKGNHIITTAIEHPSVLNTVQYLHDRYKFDVTFVKPNADGTFPAALIEKNLRPDTILVSMMWANNETGQLLPVKEV
GELLSQHQAVFHVDATQVMGKIPVHPLEIGADFLSASAHKFHGPKGVGFLYYNELLHFDALIHGGEQEEKRRAGTENLHS
LVGLAKALELALENIESNYQAVEELNHHLLSKLKEQEIPFYKNNFGPSMPHVLNLAFPNENHDLLLTKLDLAGFAISTGS
ACTAGTIEPSHVLEAVYGKNSDKLKENIRISFSELNTLDEVNLFVEKLSSILK
>Mature_292_residues
ANRDKGNHIITTAIEHPSVLNTVQYLHDRYKFDVTFVKPNADGTFPAALIEKNLRPDTILVSMMWANNETGQLLPVKEVG
ELLSQHQAVFHVDATQVMGKIPVHPLEIGADFLSASAHKFHGPKGVGFLYYNELLHFDALIHGGEQEEKRRAGTENLHSL
VGLAKALELALENIESNYQAVEELNHHLLSKLKEQEIPFYKNNFGPSMPHVLNLAFPNENHDLLLTKLDLAGFAISTGSA
CTAGTIEPSHVLEAVYGKNSDKLKENIRISFSELNTLDEVNLFVEKLSSILK

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=289, Percent_Identity=35.2941176470588, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI156713448, Length=302, Percent_Identity=31.7880794701987, Blast_Score=117, Evalue=1e-26,
Organism=Escherichia coli, GI48994898, Length=284, Percent_Identity=35.5633802816901, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1787970, Length=314, Percent_Identity=27.3885350318471, Blast_Score=72, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI25143064, Length=278, Percent_Identity=34.1726618705036, Blast_Score=161, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17533177, Length=293, Percent_Identity=34.1296928327645, Blast_Score=127, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6319831, Length=288, Percent_Identity=33.6805555555556, Blast_Score=156, Evalue=3e-39,
Organism=Drosophila melanogaster, GI20129463, Length=287, Percent_Identity=34.4947735191638, Blast_Score=159, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 32577; Mature: 32446

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANRDKGNHIITTAIEHPSVLNTVQYLHDRYKFDVTFVKPNADGTFPAALIEKNLRPDTI
CCCCCCCCEEEEEECCCCHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHHHHCCCCCCEE
LVSMMWANNETGQLLPVKEVGELLSQHQAVFHVDATQVMGKIPVHPLEIGADFLSASAHK
EEEEEECCCCCCCEEEHHHHHHHHHHCHHEEEECHHHHHHCCCCCHHHHCHHHHHCCHHH
FHGPKGVGFLYYNELLHFDALIHGGEQEEKRRAGTENLHSLVGLAKALELALENIESNYQ
CCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AVEELNHHLLSKLKEQEIPFYKNNFGPSMPHVLNLAFPNENHDLLLTKLDLAGFAISTGS
HHHHHHHHHHHHHHHHCCCCEECCCCCCCCCEEEEECCCCCCCEEEEEEEECCEEEECCC
ACTAGTIEPSHVLEAVYGKNSDKLKENIRISFSELNTLDEVNLFVEKLSSILK
CCCCCCCCHHHHHHHHHCCCHHHHHHHHEEEHHHHCCHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
ANRDKGNHIITTAIEHPSVLNTVQYLHDRYKFDVTFVKPNADGTFPAALIEKNLRPDTI
CCCCCCCEEEEEECCCCHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHHHHCCCCCCEE
LVSMMWANNETGQLLPVKEVGELLSQHQAVFHVDATQVMGKIPVHPLEIGADFLSASAHK
EEEEEECCCCCCCEEEHHHHHHHHHHCHHEEEECHHHHHHCCCCCHHHHCHHHHHCCHHH
FHGPKGVGFLYYNELLHFDALIHGGEQEEKRRAGTENLHSLVGLAKALELALENIESNYQ
CCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AVEELNHHLLSKLKEQEIPFYKNNFGPSMPHVLNLAFPNENHDLLLTKLDLAGFAISTGS
HHHHHHHHHHHHHHHHCCCCEECCCCCCCCCEEEEECCCCCCCEEEEEEEECCEEEECCC
ACTAGTIEPSHVLEAVYGKNSDKLKENIRISFSELNTLDEVNLFVEKLSSILK
CCCCCCCCHHHHHHHHHCCCHHHHHHHHEEEHHHHCCHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]