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The map label for this gene is gidA [H]

Identifier: 125624808

GI number: 125624808

Start: 2018737

End: 2020614

Strand: Reverse

Name: gidA [H]

Synonym: llmg_2035

Alternate gene names: 125624808

Gene position: 2020614-2018737 (Counterclockwise)

Preceding gene: 125624809

Following gene: 125624807

Centisome position: 79.88

GC content: 40.04

Gene sequence:

>1878_bases
ATGAATTTTCAAGAAAATTATGATGTCATCGTCATCGGAGGAGGACATGCGGGTGTTGAAGCTTCGCTTGCGGCTGCACG
CATGGGTTCTAAAACGTTATTGATGACGATTAATCTTAACATGGTTGCCTTTATGCCATGTAATCCATCAATTGGTGGCT
CAGCTAAAGGGATTGTTGTCAGAGAAATTGATGCCCTAGGTGGGGAAATGGGACGCAATATTGACAAGACCTATATTCAA
ATGAAGATGCTTAATACAGGGAAAGGTCCAGCAGTTCGTGCCCTCCGTGCCCAAGCAGATAAAGATGAATATGCTGCTTC
AATGAAAAATACAGTTTCTGACCAAGAAAACTTGACCCTTCGTCAGGGAATGGTTGAAGAACTCATCCTTGATGATGAAA
AGCAAAAAGTTATCGGTGTTCGGACATCAACAGGAACACAATACGGGGCTAAGGCTGTCATCATCACGACTGGGACAGCT
TTACGTGGGGAAATTATCATCGGTGAATTGAAATATTCTTCTGGACCAAATAATTCTCTTTCATCAATTGGTTTAGCAGA
TAATTTACGTGAAATTGGTTTTGAAATTGGTCGTTTTAAAACTGGAACACCGCCACGAGTTTTGGCAAGTTCAATTGATT
ACGATAAAACAGAAATCCAACCAGGAGATGAAGCACCAAATCATTTTAGCTTTATGAGCTCTGACGAGGATTATCTAAAA
GACCAAATTCCTTGCTGGTTGACCTATACTACAGAAAATAGTCATACGATTTTGCGTGATAATTTGCACCGTGCACCACT
TTTTTCTGGTATTGTTAAAGGAGTAGGTCCGCGCTATTGCCCATCCATAGAAGACAAAATTACTCGTTTTGCTGATAAAC
CTCGTCATCAACTCTTTCTTGAACCAGAAGGACGCAATACAGAAGAAGTTTATATCGGTGGACTTTCAACTTCAATGCCT
GAAGATGTTCAATTTGATTTAGTAAAATCAATTCCAGGACTCGAAAATGCTAAGATGATGCGTCCAGGTTATGCCATAGA
ATATGATGTGGTGATGCCTCATCAACTCCGTCCTACTCTTGAAACGAAATTGATTTCAGGTCTATTTACAGCCGGACAAA
CAAATGGAACTTCAGGTTATGAAGAAGCGGCTGGCCAAGGTTTGGTTGCCGGAATAAATGCCGCTCTTAAGATTCAAGGA
AAACCAGAATTTATTCTTAAACGCAGCGAAGCTTATATCGGTGTAATGATTGATGATTTGGTAACCAAAGGGACTTTAGA
ACCTTATCGCCTCTTGACTTCACGGGCTGAGTACCGTTTGATACTTCGTCATGATAATGCTGACCGCCGTTTGACAGAAA
TCGGACGTCAAGTTGGTCTCGTTTCAGATGCACAATGGGAGCATTACCAAGCTAAAATGGCTCAATTTGACCGAGAAATG
AAACGACTCAATTCGGAAAAACTCAAACCTTTGCCTGATACTCAAGAAAAATTAGGAAAATTGGGCTTTGGTCCAATCAA
AGATGCACTGACAGGTGCCGAATTTTTGAAACGACCAGAAGTTAATTATGATGAAGTGATTGATTTCATTGGACAAGCAC
CAGAAGTAATTGACCGCACAGTCATTGAATTGATTGAAACTGAAATTACTTACGAAGGTTACATCAAAAAAGCAATGGAC
CAAGTGGACAAAATGCATCGCTTGGAAGCAAAACGAATTCCTAAAAATATGGATTGGGATAAGTTAGATTCCATCGCTAC
CGAGGCTCGTCAAAAATTCAAAAAAATAAATCCAGAAACACTCGGACAAGCCAGCCGAATTTCAGGTGTAAATCCAGCTG
ATATTAGTATTCTTATGGTTTATCTTGAAGGAAAATAA

Upstream 100 bases:

>100_bases
AAAAGAATTGTCAGTAAGTTTGGCAGTTCTGTCAGCAGTTTTGCAAAAACTTTTATCAAAATAAAAAGAAAGGCGAATTT
TAAAAAATTCGTAGTAAAAA

Downstream 100 bases:

>100_bases
TTTAGAATTAAAAAGTTACTGACAAAAAGTTACTGATAGAAGAGTTAGAGGAGTGCCCTTGTGTTTTAATCACTTAGGAC
TAGACTCAACTTTTCATCAG

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 625; Mature: 625

Protein sequence:

>625_residues
MNFQENYDVIVIGGGHAGVEASLAAARMGSKTLLMTINLNMVAFMPCNPSIGGSAKGIVVREIDALGGEMGRNIDKTYIQ
MKMLNTGKGPAVRALRAQADKDEYAASMKNTVSDQENLTLRQGMVEELILDDEKQKVIGVRTSTGTQYGAKAVIITTGTA
LRGEIIIGELKYSSGPNNSLSSIGLADNLREIGFEIGRFKTGTPPRVLASSIDYDKTEIQPGDEAPNHFSFMSSDEDYLK
DQIPCWLTYTTENSHTILRDNLHRAPLFSGIVKGVGPRYCPSIEDKITRFADKPRHQLFLEPEGRNTEEVYIGGLSTSMP
EDVQFDLVKSIPGLENAKMMRPGYAIEYDVVMPHQLRPTLETKLISGLFTAGQTNGTSGYEEAAGQGLVAGINAALKIQG
KPEFILKRSEAYIGVMIDDLVTKGTLEPYRLLTSRAEYRLILRHDNADRRLTEIGRQVGLVSDAQWEHYQAKMAQFDREM
KRLNSEKLKPLPDTQEKLGKLGFGPIKDALTGAEFLKRPEVNYDEVIDFIGQAPEVIDRTVIELIETEITYEGYIKKAMD
QVDKMHRLEAKRIPKNMDWDKLDSIATEARQKFKKINPETLGQASRISGVNPADISILMVYLEGK

Sequences:

>Translated_625_residues
MNFQENYDVIVIGGGHAGVEASLAAARMGSKTLLMTINLNMVAFMPCNPSIGGSAKGIVVREIDALGGEMGRNIDKTYIQ
MKMLNTGKGPAVRALRAQADKDEYAASMKNTVSDQENLTLRQGMVEELILDDEKQKVIGVRTSTGTQYGAKAVIITTGTA
LRGEIIIGELKYSSGPNNSLSSIGLADNLREIGFEIGRFKTGTPPRVLASSIDYDKTEIQPGDEAPNHFSFMSSDEDYLK
DQIPCWLTYTTENSHTILRDNLHRAPLFSGIVKGVGPRYCPSIEDKITRFADKPRHQLFLEPEGRNTEEVYIGGLSTSMP
EDVQFDLVKSIPGLENAKMMRPGYAIEYDVVMPHQLRPTLETKLISGLFTAGQTNGTSGYEEAAGQGLVAGINAALKIQG
KPEFILKRSEAYIGVMIDDLVTKGTLEPYRLLTSRAEYRLILRHDNADRRLTEIGRQVGLVSDAQWEHYQAKMAQFDREM
KRLNSEKLKPLPDTQEKLGKLGFGPIKDALTGAEFLKRPEVNYDEVIDFIGQAPEVIDRTVIELIETEITYEGYIKKAMD
QVDKMHRLEAKRIPKNMDWDKLDSIATEARQKFKKINPETLGQASRISGVNPADISILMVYLEGK
>Mature_625_residues
MNFQENYDVIVIGGGHAGVEASLAAARMGSKTLLMTINLNMVAFMPCNPSIGGSAKGIVVREIDALGGEMGRNIDKTYIQ
MKMLNTGKGPAVRALRAQADKDEYAASMKNTVSDQENLTLRQGMVEELILDDEKQKVIGVRTSTGTQYGAKAVIITTGTA
LRGEIIIGELKYSSGPNNSLSSIGLADNLREIGFEIGRFKTGTPPRVLASSIDYDKTEIQPGDEAPNHFSFMSSDEDYLK
DQIPCWLTYTTENSHTILRDNLHRAPLFSGIVKGVGPRYCPSIEDKITRFADKPRHQLFLEPEGRNTEEVYIGGLSTSMP
EDVQFDLVKSIPGLENAKMMRPGYAIEYDVVMPHQLRPTLETKLISGLFTAGQTNGTSGYEEAAGQGLVAGINAALKIQG
KPEFILKRSEAYIGVMIDDLVTKGTLEPYRLLTSRAEYRLILRHDNADRRLTEIGRQVGLVSDAQWEHYQAKMAQFDREM
KRLNSEKLKPLPDTQEKLGKLGFGPIKDALTGAEFLKRPEVNYDEVIDFIGQAPEVIDRTVIELIETEITYEGYIKKAMD
QVDKMHRLEAKRIPKNMDWDKLDSIATEARQKFKKINPETLGQASRISGVNPADISILMVYLEGK

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=641, Percent_Identity=41.185647425897, Blast_Score=443, Evalue=1e-124,
Organism=Homo sapiens, GI19882217, Length=666, Percent_Identity=39.6396396396396, Blast_Score=429, Evalue=1e-120,
Organism=Homo sapiens, GI183227703, Length=681, Percent_Identity=38.7665198237885, Blast_Score=424, Evalue=1e-118,
Organism=Escherichia coli, GI2367273, Length=623, Percent_Identity=48.1540930979133, Blast_Score=609, Evalue=1e-175,
Organism=Caenorhabditis elegans, GI17534255, Length=624, Percent_Identity=39.7435897435897, Blast_Score=444, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6321202, Length=620, Percent_Identity=43.0645161290323, Blast_Score=481, Evalue=1e-136,
Organism=Drosophila melanogaster, GI24658174, Length=637, Percent_Identity=41.6012558869702, Blast_Score=441, Evalue=1e-124,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 69336; Mature: 69336

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS01280 GIDA_1 ; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFQENYDVIVIGGGHAGVEASLAAARMGSKTLLMTINLNMVAFMPCNPSIGGSAKGIVV
CCCCCCCCEEEEECCCCCCHHHHHHHHCCCCEEEEEEECCEEEEECCCCCCCCCCCCEEE
REIDALGGEMGRNIDKTYIQMKMLNTGKGPAVRALRAQADKDEYAASMKNTVSDQENLTL
EEHHHHHHHHCCCCCHHEEEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHH
RQGMVEELILDDEKQKVIGVRTSTGTQYGAKAVIITTGTALRGEIIIGELKYSSGPNNSL
HHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEEEEECCCCEECEEEEEEEEECCCCCCCH
SSIGLADNLREIGFEIGRFKTGTPPRVLASSIDYDKTEIQPGDEAPNHFSFMSSDEDYLK
HHCCHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCEECCCCHHHHH
DQIPCWLTYTTENSHTILRDNLHRAPLFSGIVKGVGPRYCPSIEDKITRFADKPRHQLFL
CCCCEEEEEECCCCCEEHHCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEE
EPEGRNTEEVYIGGLSTSMPEDVQFDLVKSIPGLENAKMMRPGYAIEYDVVMPHQLRPTL
CCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCCCEECCCCEEEEEEECCCCCCCHH
ETKLISGLFTAGQTNGTSGYEEAAGQGLVAGINAALKIQGKPEFILKRSEAYIGVMIDDL
HHHHHHHHHHCCCCCCCCCHHHHCCCCEEEECCEEEEECCCCCEEEEECCCEEEEEHHHH
VTKGTLEPYRLLTSRAEYRLILRHDNADRRLTEIGRQVGLVSDAQWEHYQAKMAQFDREM
HHCCCCCHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
KRLNSEKLKPLPDTQEKLGKLGFGPIKDALTGAEFLKRPEVNYDEVIDFIGQAPEVIDRT
HHHCHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHH
VIELIETEITYEGYIKKAMDQVDKMHRLEAKRIPKNMDWDKLDSIATEARQKFKKINPET
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHH
LGQASRISGVNPADISILMVYLEGK
CCCHHHCCCCCCCCEEEEEEEEECC
>Mature Secondary Structure
MNFQENYDVIVIGGGHAGVEASLAAARMGSKTLLMTINLNMVAFMPCNPSIGGSAKGIVV
CCCCCCCCEEEEECCCCCCHHHHHHHHCCCCEEEEEEECCEEEEECCCCCCCCCCCCEEE
REIDALGGEMGRNIDKTYIQMKMLNTGKGPAVRALRAQADKDEYAASMKNTVSDQENLTL
EEHHHHHHHHCCCCCHHEEEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHH
RQGMVEELILDDEKQKVIGVRTSTGTQYGAKAVIITTGTALRGEIIIGELKYSSGPNNSL
HHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEEEEECCCCEECEEEEEEEEECCCCCCCH
SSIGLADNLREIGFEIGRFKTGTPPRVLASSIDYDKTEIQPGDEAPNHFSFMSSDEDYLK
HHCCHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCEECCCCHHHHH
DQIPCWLTYTTENSHTILRDNLHRAPLFSGIVKGVGPRYCPSIEDKITRFADKPRHQLFL
CCCCEEEEEECCCCCEEHHCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEE
EPEGRNTEEVYIGGLSTSMPEDVQFDLVKSIPGLENAKMMRPGYAIEYDVVMPHQLRPTL
CCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCCCEECCCCEEEEEEECCCCCCCHH
ETKLISGLFTAGQTNGTSGYEEAAGQGLVAGINAALKIQGKPEFILKRSEAYIGVMIDDL
HHHHHHHHHHCCCCCCCCCHHHHCCCCEEEECCEEEEECCCCCEEEEECCCEEEEEHHHH
VTKGTLEPYRLLTSRAEYRLILRHDNADRRLTEIGRQVGLVSDAQWEHYQAKMAQFDREM
HHCCCCCHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
KRLNSEKLKPLPDTQEKLGKLGFGPIKDALTGAEFLKRPEVNYDEVIDFIGQAPEVIDRT
HHHCHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHH
VIELIETEITYEGYIKKAMDQVDKMHRLEAKRIPKNMDWDKLDSIATEARQKFKKINPET
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHH
LGQASRISGVNPADISILMVYLEGK
CCCHHHCCCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]