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The map label for this gene is thiN [H]

Identifier: 125624788

GI number: 125624788

Start: 2000399

End: 2001034

Strand: Reverse

Name: thiN [H]

Synonym: llmg_2013

Alternate gene names: 125624788

Gene position: 2001034-2000399 (Counterclockwise)

Preceding gene: 125624789

Following gene: 125624787

Centisome position: 79.11

GC content: 35.69

Gene sequence:

>636_bases
ATGAAAATATTAATTGTAGCAGGCTTGCCTGATTTTATTCCCGACGAAACTTTTGATAAATATATTGGAGTGGATCGTGG
CTCGCTTTTTTTAGTGGAAAAAGGGATTCAGTTAACGCTTGCGATTGGTGATTTTGATTCTGTCAGTAAAACTGAACTTG
ATAAAATTTCTGTCAGTACTGACAAGTTGATTAAATTACCTGCTGAAAAAGATTTGACAGATTTAGAAGCAGCTTTAGAT
TTTATATTAAAAGATTTTCCAGATGCTGAGCTGACAATAGCTGGTGCTTTAGGCGGACGTTTGGATCACTTACTGACCAA
TGCCTACCTAGCAACACGGCCAAAATATCAAAGTCTTGCTCCCAAAATGCATTTAGTTGACCAACAAAATTTGGTGACCT
ATTTACTACCAGGTCAGCATTTACTCAAAAGAATACCAACTTACAAATATATTGGTTTTGTACAGGTCGATACAAAAGAT
AGTCTGGCTATTGTGGGAGCCAAGTATCCTTTGAAAGCCGAAGATAATTTTAGTTTAATCTATGCAAGTAATGAGTTTAT
AAGTGAGCAGATGCAAGTTTCATTTGATAGTGGAGTGGTCATTGTGATTTATTCAGGAGATTTGCGGAAAAAATAA

Upstream 100 bases:

>100_bases
GTTATTTAGGAATATCCTTAGAACAAGTTGCAAGCGAAAACATTGAAAAATTAGAAAGTCGTTTAAATCGTGGAAAAATT
TCAGGATCAGGTGATGAAAG

Downstream 100 bases:

>100_bases
GTATCATCAGATTTAAGGAGAGAAAAAGTATGATTATTGGAGTCATTGGGGCAACATCAAATATCGCAAGCAAAGCTTAT
TTACCTGTTTATGCAAAAAT

Product: thiamin pyrophosphokinase

Products: NA

Alternate protein names: TPK; Thiamine diphosphokinase [H]

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MKILIVAGLPDFIPDETFDKYIGVDRGSLFLVEKGIQLTLAIGDFDSVSKTELDKISVSTDKLIKLPAEKDLTDLEAALD
FILKDFPDAELTIAGALGGRLDHLLTNAYLATRPKYQSLAPKMHLVDQQNLVTYLLPGQHLLKRIPTYKYIGFVQVDTKD
SLAIVGAKYPLKAEDNFSLIYASNEFISEQMQVSFDSGVVIVIYSGDLRKK

Sequences:

>Translated_211_residues
MKILIVAGLPDFIPDETFDKYIGVDRGSLFLVEKGIQLTLAIGDFDSVSKTELDKISVSTDKLIKLPAEKDLTDLEAALD
FILKDFPDAELTIAGALGGRLDHLLTNAYLATRPKYQSLAPKMHLVDQQNLVTYLLPGQHLLKRIPTYKYIGFVQVDTKD
SLAIVGAKYPLKAEDNFSLIYASNEFISEQMQVSFDSGVVIVIYSGDLRKK
>Mature_211_residues
MKILIVAGLPDFIPDETFDKYIGVDRGSLFLVEKGIQLTLAIGDFDSVSKTELDKISVSTDKLIKLPAEKDLTDLEAALD
FILKDFPDAELTIAGALGGRLDHLLTNAYLATRPKYQSLAPKMHLVDQQNLVTYLLPGQHLLKRIPTYKYIGFVQVDTKD
SLAIVGAKYPLKAEDNFSLIYASNEFISEQMQVSFDSGVVIVIYSGDLRKK

Specific function: Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate [H]

COG id: COG1564

COG function: function code H; Thiamine pyrophosphokinase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine pyrophosphokinase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006282
- InterPro:   IPR007373
- InterPro:   IPR007371 [H]

Pfam domain/function: PF04263 TPK_catalytic [H]

EC number: =2.7.6.2 [H]

Molecular weight: Translated: 23429; Mature: 23429

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILIVAGLPDFIPDETFDKYIGVDRGSLFLVEKGIQLTLAIGDFDSVSKTELDKISVST
CEEEEEECCCCCCCCCHHHHHHCCCCCCEEEEECCCEEEEEECCCCCCCCCCHHEEECCC
DKLIKLPAEKDLTDLEAALDFILKDFPDAELTIAGALGGRLDHLLTNAYLATRPKYQSLA
CCEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEEHHCCHHHHHHHHHHHHCCCCHHHHC
PKMHLVDQQNLVTYLLPGQHLLKRIPTYKYIGFVQVDTKDSLAIVGAKYPLKAEDNFSLI
CCEEEECCCCEEEEEECCHHHHHHCCCEEEEEEEEECCCCCEEEEECCCCCCCCCCEEEE
YASNEFISEQMQVSFDSGVVIVIYSGDLRKK
EECCHHHHHHHEEEECCCEEEEEECCCCCCC
>Mature Secondary Structure
MKILIVAGLPDFIPDETFDKYIGVDRGSLFLVEKGIQLTLAIGDFDSVSKTELDKISVST
CEEEEEECCCCCCCCCHHHHHHCCCCCCEEEEECCCEEEEEECCCCCCCCCCHHEEECCC
DKLIKLPAEKDLTDLEAALDFILKDFPDAELTIAGALGGRLDHLLTNAYLATRPKYQSLA
CCEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEEHHCCHHHHHHHHHHHHCCCCHHHHC
PKMHLVDQQNLVTYLLPGQHLLKRIPTYKYIGFVQVDTKDSLAIVGAKYPLKAEDNFSLI
CCEEEECCCCEEEEEECCHHHHHHCCCEEEEEEEEECCCCCEEEEECCCCCCCCCCEEEE
YASNEFISEQMQVSFDSGVVIVIYSGDLRKK
EECCHHHHHHHEEEECCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377 [H]