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The map label for this gene is pflA [H]

Identifier: 125624774

GI number: 125624774

Start: 1986117

End: 1986908

Strand: Reverse

Name: pflA [H]

Synonym: llmg_1997

Alternate gene names: 125624774

Gene position: 1986908-1986117 (Counterclockwise)

Preceding gene: 125624775

Following gene: 125624773

Centisome position: 78.55

GC content: 36.62

Gene sequence:

>792_bases
ATGTCAGAAAATATAGATGAACTAAAAAAAGTGACCGGACTGATTCATTCAACTGAATCATTTGGTTCTGTTGATGGGCC
TGGTGTACGATTTATTATCTTTATGCAAGGTTGTCGGATGCGTTGTAAGTATTGTCATAACCCTGATACTTGGGCTTTGA
AATCGGATAAAGCCACAAAACGGACTGTAGAAGATGTCATGGATGAGGCGCTTCGCTTTAGGGGATTCTGGGGAGAAAAA
GGGGGAATTACTGTTTCTGGTGGTGAAGCCCTTCTTCAAATTGACTTCGTTTTGGCTCTTTTTAAATATGCAAAATCTCT
TGGTATTCATACAACGCTTGATACAGCAGCTCAACCTTATTTGACTGACAAATATGTGACTGGAAAAATTGATGAATTAT
TAGATTATACTGATTTGGTCTTGTTGGACATCAAAGAAATCAATCCAGAACGTCATAAAGAACTGACAGCTAACAAAAAT
GACAATATTTTGGCTTTTGCCCAATATTTATCAGACCGTGGCAATGCCATGTGGGTTCGTCATGTTCTTGTCCCTGGTGA
AAGTGATTTTGATGAAGATTTGGTACAATTGGGTGAATTTGTTAAGACTTTGAAAAATGTTTTAAAATTTGAAATTTTAC
CCTACCATACAATGGGTGAATTTAAATGGCGTGAATTAGGTTGGAAATATCCACTTGAAGGGGTTAAACCGCCAACGAAA
GACCGTGTTCATAATGCTAAAGAAATCATGAATACAGAGTCTTATCAAGACTATTTAGAACGAATAAAATAA

Upstream 100 bases:

>100_bases
GCCGTTAGTAGGATATTTTACTGACAGAATTTAATTTGATAAAATCAGCGAATTGCGGTATGATAGTGGTGTGAAATAGT
GTTTGAAATTGGAGAAAATG

Downstream 100 bases:

>100_bases
AAAATAAAATGGATTTGAAAATATTTCAAATCCATTTTTTTTTATAACCGAACAAATAAGCAGAAAGAGACTTAAATGAA
AATGAAAGTGTGCTTAATCA

Product: pyruvate-formate lyase activating enzyme

Products: NA

Alternate protein names: PFL-activating enzyme; Formate-C-acetyltransferase-activating enzyme [H]

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MSENIDELKKVTGLIHSTESFGSVDGPGVRFIIFMQGCRMRCKYCHNPDTWALKSDKATKRTVEDVMDEALRFRGFWGEK
GGITVSGGEALLQIDFVLALFKYAKSLGIHTTLDTAAQPYLTDKYVTGKIDELLDYTDLVLLDIKEINPERHKELTANKN
DNILAFAQYLSDRGNAMWVRHVLVPGESDFDEDLVQLGEFVKTLKNVLKFEILPYHTMGEFKWRELGWKYPLEGVKPPTK
DRVHNAKEIMNTESYQDYLERIK

Sequences:

>Translated_263_residues
MSENIDELKKVTGLIHSTESFGSVDGPGVRFIIFMQGCRMRCKYCHNPDTWALKSDKATKRTVEDVMDEALRFRGFWGEK
GGITVSGGEALLQIDFVLALFKYAKSLGIHTTLDTAAQPYLTDKYVTGKIDELLDYTDLVLLDIKEINPERHKELTANKN
DNILAFAQYLSDRGNAMWVRHVLVPGESDFDEDLVQLGEFVKTLKNVLKFEILPYHTMGEFKWRELGWKYPLEGVKPPTK
DRVHNAKEIMNTESYQDYLERIK
>Mature_262_residues
SENIDELKKVTGLIHSTESFGSVDGPGVRFIIFMQGCRMRCKYCHNPDTWALKSDKATKRTVEDVMDEALRFRGFWGEKG
GITVSGGEALLQIDFVLALFKYAKSLGIHTTLDTAAQPYLTDKYVTGKIDELLDYTDLVLLDIKEINPERHKELTANKND
NILAFAQYLSDRGNAMWVRHVLVPGESDFDEDLVQLGEFVKTLKNVLKFEILPYHTMGEFKWRELGWKYPLEGVKPPTKD
RVHNAKEIMNTESYQDYLERIK

Specific function: Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=247, Percent_Identity=47.7732793522267, Blast_Score=228, Evalue=3e-61,
Organism=Escherichia coli, GI1790839, Length=254, Percent_Identity=27.9527559055118, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1790389, Length=271, Percent_Identity=23.6162361623616, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 30190; Mature: 30059

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSENIDELKKVTGLIHSTESFGSVDGPGVRFIIFMQGCRMRCKYCHNPDTWALKSDKATK
CCCCHHHHHHHHHHHHCCHHCCCCCCCCEEEEEEECCHHHHHHCCCCCCCEEECCCCHHH
RTVEDVMDEALRFRGFWGEKGGITVSGGEALLQIDFVLALFKYAKSLGIHTTLDTAAQPY
HHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCC
LTDKYVTGKIDELLDYTDLVLLDIKEINPERHKELTANKNDNILAFAQYLSDRGNAMWVR
CCCCCCCCCHHHHHHHHHHHEEEHHHCCHHHHHHCCCCCCCCEEHHHHHHHCCCCEEEEE
HVLVPGESDFDEDLVQLGEFVKTLKNVLKFEILPYHTMGEFKWRELGWKYPLEGVKPPTK
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCH
DRVHNAKEIMNTESYQDYLERIK
HHHHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure 
SENIDELKKVTGLIHSTESFGSVDGPGVRFIIFMQGCRMRCKYCHNPDTWALKSDKATK
CCCHHHHHHHHHHHHCCHHCCCCCCCCEEEEEEECCHHHHHHCCCCCCCEEECCCCHHH
RTVEDVMDEALRFRGFWGEKGGITVSGGEALLQIDFVLALFKYAKSLGIHTTLDTAAQPY
HHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCC
LTDKYVTGKIDELLDYTDLVLLDIKEINPERHKELTANKNDNILAFAQYLSDRGNAMWVR
CCCCCCCCCHHHHHHHHHHHEEEHHHCCHHHHHHCCCCCCCCEEHHHHHHHCCCCEEEEE
HVLVPGESDFDEDLVQLGEFVKTLKNVLKFEILPYHTMGEFKWRELGWKYPLEGVKPPTK
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCH
DRVHNAKEIMNTESYQDYLERIK
HHHHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11029425; 10899886; 12397186 [H]