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The map label for this gene is birA1 [H]

Identifier: 125624741

GI number: 125624741

Start: 1944255

End: 1945226

Strand: Reverse

Name: birA1 [H]

Synonym: llmg_1963

Alternate gene names: 125624741

Gene position: 1945226-1944255 (Counterclockwise)

Preceding gene: 125624742

Following gene: 125624740

Centisome position: 76.9

GC content: 34.26

Gene sequence:

>972_bases
ATGCAAACAACAAAACAATTTGTCTTGTTAGAACTTCTTCGACAAAAAGGGAATTGGGTTTCAGGAGATGAATTAGCAGA
AGAGCTAAATATTAGTCGTGAAAGTATTTGGAAAGCAATCAATACTTTAAAACGTAAAGGAAATCAGATTGTCAGCCGAA
AAAATTTAGGATATAAGTATATTGGCAATGAATCATTAGACGAAGATACGATAAATTTTTATAGTGGAATGAGATTTGAA
AATGATATTTTCGTTTTTGATGAAGTGACTTCTACACAGGAAGTGGCAAAACAATATTTGACGACTCATGAGGTCAAACA
GCCGCTTGTTTTTGTTGCGAATAATCAATCTCAAGGACATGGGAGGCGCGGTCGAAAATTTTATTCGCCAAGTGATACTG
GACTATATTTTAGTCTTATTTTACCTAATCCCAGCCATGATATTTTGAAAATAGGTTTATTGACTACAAGTACTGCTGTT
GCCGTGGTCAAAGTACTTGAAAAATTTTATGAGGATAAAAACTTTAAGTTAAAGTGGGTTAATGATATTTATCTAGATAC
TTATAAAGTGGGAGGTATTATCACAGAAGCGGCTTTAGATTTAGAATCGAGTTCTGCTAGCAATTTTATTATGGGAATTG
GCTTAAATTTGGCAACTAAGGAATTTCCAGATGAGTTATTAGGAAAAGTGAAAGGAATTAATCCCGATATAAATATAGAC
CGGAATCAATTGATTGCAGAAATGGCTCAGGAAGTAATTGCTAATTTTTCAAATTATACTCACCCGAACTTGATTGAAGA
ATATCGCAAGCGCTCTTTATTAGTCGGAAAAGAGGTTACTTTGAAATTAGGAACGAGTTCTGTCAAAGGAGAAGTACAAG
GAATTGCTGATGACGGAAGTTTAGTACTCAAAGGGGATTCAGGAAAGCTACAATCTTTCACGAGTGGTGAGGTTATAAAA
GTTGATTGGTAA

Upstream 100 bases:

>100_bases
TTATTCCAGGAGACTGTATCAAAGCCATTTTAACGGTCGTCATCGTTCGACGCCTTAGAAAGCAAGGAGGTTTTGAACTT
TATTTTAGAAAGTGATAGTT

Downstream 100 bases:

>100_bases
GAGAAGTAGGAGATATACTATTGGAAAAAATTTGGGGAAAATTGGTAGATGATGAGGGAAGATGCAGTCATTATCATCAA
GAAAATGATATTGTTGCATT

Product: biotin--[acetyl-CoA-carboxylase] ligase and biotin operon repressor

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MQTTKQFVLLELLRQKGNWVSGDELAEELNISRESIWKAINTLKRKGNQIVSRKNLGYKYIGNESLDEDTINFYSGMRFE
NDIFVFDEVTSTQEVAKQYLTTHEVKQPLVFVANNQSQGHGRRGRKFYSPSDTGLYFSLILPNPSHDILKIGLLTTSTAV
AVVKVLEKFYEDKNFKLKWVNDIYLDTYKVGGIITEAALDLESSSASNFIMGIGLNLATKEFPDELLGKVKGINPDINID
RNQLIAEMAQEVIANFSNYTHPNLIEEYRKRSLLVGKEVTLKLGTSSVKGEVQGIADDGSLVLKGDSGKLQSFTSGEVIK
VDW

Sequences:

>Translated_323_residues
MQTTKQFVLLELLRQKGNWVSGDELAEELNISRESIWKAINTLKRKGNQIVSRKNLGYKYIGNESLDEDTINFYSGMRFE
NDIFVFDEVTSTQEVAKQYLTTHEVKQPLVFVANNQSQGHGRRGRKFYSPSDTGLYFSLILPNPSHDILKIGLLTTSTAV
AVVKVLEKFYEDKNFKLKWVNDIYLDTYKVGGIITEAALDLESSSASNFIMGIGLNLATKEFPDELLGKVKGINPDINID
RNQLIAEMAQEVIANFSNYTHPNLIEEYRKRSLLVGKEVTLKLGTSSVKGEVQGIADDGSLVLKGDSGKLQSFTSGEVIK
VDW
>Mature_323_residues
MQTTKQFVLLELLRQKGNWVSGDELAEELNISRESIWKAINTLKRKGNQIVSRKNLGYKYIGNESLDEDTINFYSGMRFE
NDIFVFDEVTSTQEVAKQYLTTHEVKQPLVFVANNQSQGHGRRGRKFYSPSDTGLYFSLILPNPSHDILKIGLLTTSTAV
AVVKVLEKFYEDKNFKLKWVNDIYLDTYKVGGIITEAALDLESSSASNFIMGIGLNLATKEFPDELLGKVKGINPDINID
RNQLIAEMAQEVIANFSNYTHPNLIEEYRKRSLLVGKEVTLKLGTSSVKGEVQGIADDGSLVLKGDSGKLQSFTSGEVIK
VDW

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=309, Percent_Identity=28.8025889967638, Blast_Score=107, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI32563944, Length=313, Percent_Identity=23.3226837060703, Blast_Score=68, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI32563942, Length=313, Percent_Identity=23.3226837060703, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI212645369, Length=313, Percent_Identity=23.3226837060703, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 36245; Mature: 36245

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTTKQFVLLELLRQKGNWVSGDELAEELNISRESIWKAINTLKRKGNQIVSRKNLGYKY
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHCCCCEEE
IGNESLDEDTINFYSGMRFENDIFVFDEVTSTQEVAKQYLTTHEVKQPLVFVANNQSQGH
CCCCCCCCHHHHHHHCCEECCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCC
GRRGRKFYSPSDTGLYFSLILPNPSHDILKIGLLTTSTAVAVVKVLEKFYEDKNFKLKWV
CCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEE
NDIYLDTYKVGGIITEAALDLESSSASNFIMGIGLNLATKEFPDELLGKVKGINPDINID
CEEEEEEEHHCCEEEHHHHCCCCCCCCCEEEEECCCHHHHCCCHHHHHHHCCCCCCCCCC
RNQLIAEMAQEVIANFSNYTHPNLIEEYRKRSLLVGKEVTLKLGTSSVKGEVQGIADDGS
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEECCCEEEEEECCCCCCCCEEEECCCCC
LVLKGDSGKLQSFTSGEVIKVDW
EEEECCCCCCCCCCCCCEEEECC
>Mature Secondary Structure
MQTTKQFVLLELLRQKGNWVSGDELAEELNISRESIWKAINTLKRKGNQIVSRKNLGYKY
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHCCCCEEE
IGNESLDEDTINFYSGMRFENDIFVFDEVTSTQEVAKQYLTTHEVKQPLVFVANNQSQGH
CCCCCCCCHHHHHHHCCEECCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCC
GRRGRKFYSPSDTGLYFSLILPNPSHDILKIGLLTTSTAVAVVKVLEKFYEDKNFKLKWV
CCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEE
NDIYLDTYKVGGIITEAALDLESSSASNFIMGIGLNLATKEFPDELLGKVKGINPDINID
CEEEEEEEHHCCEEEHHHHCCCCCCCCCEEEEECCCHHHHCCCHHHHHHHCCCCCCCCCC
RNQLIAEMAQEVIANFSNYTHPNLIEEYRKRSLLVGKEVTLKLGTSSVKGEVQGIADDGS
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEECCCEEEEEECCCCCCCCEEEECCCCC
LVLKGDSGKLQSFTSGEVIKVDW
EEEECCCCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]