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The map label for this gene is apu [H]

Identifier: 125624657

GI number: 125624657

Start: 1849292

End: 1851094

Strand: Reverse

Name: apu [H]

Synonym: llmg_1869

Alternate gene names: 125624657

Gene position: 1851094-1849292 (Counterclockwise)

Preceding gene: 125624659

Following gene: 125624656

Centisome position: 73.18

GC content: 33.94

Gene sequence:

>1803_bases
ATGTATTACTATAATCCATGGAATTTAGATTATAAGCAACCTTTTGGTGCGGTAAAAGTAGGGCAAGCCATTTTATTGAA
ATTTGACGCAAATCAAACTCAAGTAGAAGTGAAGTGCATTATTCGACGTGATTTTGGAAAAAGATATGAATTCCTTATGA
GCAGAGATGAGTCAGGTTTTTTTACAGTCACTATCCCATTTGATGAAAATGCTGGTCTTTATTTTTACTATTTTGAAATT
TTGGAGGGCTCAGATTGGGAACAAAACAAGCGTTTTTATGGGAGTTCTGGAATAGGTGGGGAAGGGCTGCTTTATACTAA
CGAAAATGATATTAAACCCTATCAGCTGACAGTTTTTGAAAAAGAGGACAAGGCACCAGCTTGGTATCGTGATGCTGTTT
TTTACCAGATTTTTCCTGATCGTTTCCATAATGGAAATGAGAATGGACAAGTCAGCCATCCAAAACCGAATTCTTTTATT
TATGGAACTAAATCTGATACGCCTTTTTATGTGAAAGAGGAAAATGGTGATATTGCACGTTGGGATTTCTTTGGTGGAAA
TCTAAGAGGAATCATTAACAAAATTCCATATTTAAAAGAACTTGGAATCAATGCCATTTATCTTAATCCAATTTTTTCTG
GGACAAGTAACCATCGTTATGACACCAACGATTATCTAAAAATAGATTCCATGTTAGGAACTCAAGAAGATTTTCAAGAA
TTAATCAATTTACTTCATCAAGAACAAATGCATTTAGTTTTGGACGGTGTCTTTTCACATGTAGGTAAAAATTCACTTTA
CTTCAATATCAGTGGAGATTATGGAGATGATGAAGGAGCGGCGAAAAATCCTGACTCCCCTTATTTTGACTGGTTCAAGT
TTGAAAATTATCCTTTTGATTATAAATCTTGGTGGGGAATTAAAGATTTACCTGAAATTGATAAAGATAATGAGTCCTTT
CGTGATTTTATTTATGGTAGGAAAAACTCTGTTTTATCAAAGTGGAATGACTTGGGAATAGATGGTTGGCGATTAGATGT
TGCTGATGAATTGCCTGATTCATTTATTAAAGGGATTCGTGAGAATCTTGACCGTTATTCAGAAAAAATTCTAATAGGAG
AAATTTGGGAGGATGCTTCTAATAAGATTTCTTATGGAACAAGACGCAAGTATATTCTTGGCGGGAGCTTACAGGCTTGT
ATGAACTATCCGTTTCGTGATTTAATCATCAATTTATTAAACGGAAATCGCTCATGTCAGGATGTAGCTCATCAATTGAT
GACTTTGCAGGAAAACTATCCCAAAGATATTTTTTATAACAACTTAAATAATTTGGGGACCCATGATACGGAACGTATTT
TAACAATGGTCGGAAAAGAAAATCTGCCAATAGCTGTTTCACTCTTATTTGTTTTACCAGGAATCCCTTGTATTTATTAC
GGTGACGAAGCGGGACTTTCTGGAGGAAAGGATCCAGAAAATCGTAAATATTTTCCTTGGGATGATATTTCACCAGAAAT
ATATGATTATTATAAGAGTTGGACGAAAAAGCGTCTGTTAGAAAATAGTTTGAAAGAAGGAGAATTTTCTAGCTTTTATA
GTGACAGACTACTAGGAATTTTGCGCTATACAGATTCAGAAGTTTTTGTCGAGGTGATTAATCCCAGTGAAGATGAAATC
AAGATAGATTATTCAGAAATTACTTTTTTACAGAAGTTTGATTTTATGCCAAAACTAAAAGATTTACTGACAGAGCAAGT
GATTAGTGGAAAATCAAACATAGATTTGAAAATAGAAAGATAA

Upstream 100 bases:

>100_bases
TCAAAAAACTCCTGACCGTTTGTGACAGGAGTTCTTGTTTTAATAGAAAGAACTCTCTAATTTTCTATCTTAAACTACTA
AAAAATGAAATGAGAATCAG

Downstream 100 bases:

>100_bases
AAATCTGCTTTAGCAGATTTTTGATTTACTGAAAAGTCTGTCAGTGAAAAATAATTTAAAAAGGAATACAAATGAATGGC
TGAAGATAGTCTTAAGGATT

Product: amylopullulanase

Products: NA

Alternate protein names: Alpha-amylase/pullulanase; Alpha-amylase; 1,4-alpha-D-glucan glucanohydrolase; Pullulanase; 1,4-alpha-D-glucan glucanohydrolase; Alpha-dextrin endo-1,6-alpha-glucosidase [H]

Number of amino acids: Translated: 600; Mature: 600

Protein sequence:

>600_residues
MYYYNPWNLDYKQPFGAVKVGQAILLKFDANQTQVEVKCIIRRDFGKRYEFLMSRDESGFFTVTIPFDENAGLYFYYFEI
LEGSDWEQNKRFYGSSGIGGEGLLYTNENDIKPYQLTVFEKEDKAPAWYRDAVFYQIFPDRFHNGNENGQVSHPKPNSFI
YGTKSDTPFYVKEENGDIARWDFFGGNLRGIINKIPYLKELGINAIYLNPIFSGTSNHRYDTNDYLKIDSMLGTQEDFQE
LINLLHQEQMHLVLDGVFSHVGKNSLYFNISGDYGDDEGAAKNPDSPYFDWFKFENYPFDYKSWWGIKDLPEIDKDNESF
RDFIYGRKNSVLSKWNDLGIDGWRLDVADELPDSFIKGIRENLDRYSEKILIGEIWEDASNKISYGTRRKYILGGSLQAC
MNYPFRDLIINLLNGNRSCQDVAHQLMTLQENYPKDIFYNNLNNLGTHDTERILTMVGKENLPIAVSLLFVLPGIPCIYY
GDEAGLSGGKDPENRKYFPWDDISPEIYDYYKSWTKKRLLENSLKEGEFSSFYSDRLLGILRYTDSEVFVEVINPSEDEI
KIDYSEITFLQKFDFMPKLKDLLTEQVISGKSNIDLKIER

Sequences:

>Translated_600_residues
MYYYNPWNLDYKQPFGAVKVGQAILLKFDANQTQVEVKCIIRRDFGKRYEFLMSRDESGFFTVTIPFDENAGLYFYYFEI
LEGSDWEQNKRFYGSSGIGGEGLLYTNENDIKPYQLTVFEKEDKAPAWYRDAVFYQIFPDRFHNGNENGQVSHPKPNSFI
YGTKSDTPFYVKEENGDIARWDFFGGNLRGIINKIPYLKELGINAIYLNPIFSGTSNHRYDTNDYLKIDSMLGTQEDFQE
LINLLHQEQMHLVLDGVFSHVGKNSLYFNISGDYGDDEGAAKNPDSPYFDWFKFENYPFDYKSWWGIKDLPEIDKDNESF
RDFIYGRKNSVLSKWNDLGIDGWRLDVADELPDSFIKGIRENLDRYSEKILIGEIWEDASNKISYGTRRKYILGGSLQAC
MNYPFRDLIINLLNGNRSCQDVAHQLMTLQENYPKDIFYNNLNNLGTHDTERILTMVGKENLPIAVSLLFVLPGIPCIYY
GDEAGLSGGKDPENRKYFPWDDISPEIYDYYKSWTKKRLLENSLKEGEFSSFYSDRLLGILRYTDSEVFVEVINPSEDEI
KIDYSEITFLQKFDFMPKLKDLLTEQVISGKSNIDLKIER
>Mature_600_residues
MYYYNPWNLDYKQPFGAVKVGQAILLKFDANQTQVEVKCIIRRDFGKRYEFLMSRDESGFFTVTIPFDENAGLYFYYFEI
LEGSDWEQNKRFYGSSGIGGEGLLYTNENDIKPYQLTVFEKEDKAPAWYRDAVFYQIFPDRFHNGNENGQVSHPKPNSFI
YGTKSDTPFYVKEENGDIARWDFFGGNLRGIINKIPYLKELGINAIYLNPIFSGTSNHRYDTNDYLKIDSMLGTQEDFQE
LINLLHQEQMHLVLDGVFSHVGKNSLYFNISGDYGDDEGAAKNPDSPYFDWFKFENYPFDYKSWWGIKDLPEIDKDNESF
RDFIYGRKNSVLSKWNDLGIDGWRLDVADELPDSFIKGIRENLDRYSEKILIGEIWEDASNKISYGTRRKYILGGSLQAC
MNYPFRDLIINLLNGNRSCQDVAHQLMTLQENYPKDIFYNNLNNLGTHDTERILTMVGKENLPIAVSLLFVLPGIPCIYY
GDEAGLSGGKDPENRKYFPWDDISPEIYDYYKSWTKKRLLENSLKEGEFSSFYSDRLLGILRYTDSEVFVEVINPSEDEI
KIDYSEITFLQKFDFMPKLKDLLTEQVISGKSNIDLKIER

Specific function: May Play A Role In Regulating The Intracellular Level Of Maltotriose. Cleaves Glucose From The Reducing End Of Maltotriose And Longer Maltodextrins With A Chain Length Of Up To 7 Glucose Units. [C]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 fibronectin type-III domains [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=430, Percent_Identity=20.9302325581395, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI1786604, Length=524, Percent_Identity=30.5343511450382, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI1789995, Length=530, Percent_Identity=23.3962264150943, Blast_Score=93, Evalue=6e-20,
Organism=Escherichia coli, GI1790687, Length=418, Percent_Identity=23.9234449760766, Blast_Score=89, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI32565753, Length=554, Percent_Identity=21.4801444043321, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321726, Length=250, Percent_Identity=26.4, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321731, Length=271, Percent_Identity=25.4612546125461, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319776, Length=271, Percent_Identity=25.4612546125461, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6322241, Length=250, Percent_Identity=25.6, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6322021, Length=250, Percent_Identity=25.6, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324416, Length=250, Percent_Identity=25.6, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6322245, Length=264, Percent_Identity=24.6212121212121, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24586599, Length=446, Percent_Identity=23.7668161434978, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24586587, Length=425, Percent_Identity=22.8235294117647, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24586593, Length=589, Percent_Identity=21.2224108658744, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24586591, Length=475, Percent_Identity=22.7368421052632, Blast_Score=83, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24586597, Length=418, Percent_Identity=22.7272727272727, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI221330053, Length=427, Percent_Identity=21.5456674473068, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006048
- InterPro:   IPR013784
- InterPro:   IPR008957
- InterPro:   IPR003961
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR002044
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N; PF00041 fn3 [H]

EC number: =3.2.1.1; =3.2.1.41 [H]

Molecular weight: Translated: 69957; Mature: 69957

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYYYNPWNLDYKQPFGAVKVGQAILLKFDANQTQVEVKCIIRRDFGKRYEFLMSRDESGF
CCEECCCCCCCCCCCCCEECCCEEEEEECCCCCEEEEEEEEEHHHHHHHHHHHCCCCCCE
FTVTIPFDENAGLYFYYFEILEGSDWEQNKRFYGSSGIGGEGLLYTNENDIKPYQLTVFE
EEEEEECCCCCCEEEEEEEEECCCCCCCCCCEECCCCCCCCEEEEECCCCCCCEEEEEEE
KEDKAPAWYRDAVFYQIFPDRFHNGNENGQVSHPKPNSFIYGTKSDTPFYVKEENGDIAR
CCCCCCCHHHHCEEEEECCHHHCCCCCCCEECCCCCCCEEEECCCCCCEEEEECCCCEEE
WDFFGGNLRGIINKIPYLKELGINAIYLNPIFSGTSNHRYDTNDYLKIDSMLGTQEDFQE
EEECCCCHHHHHHHCCHHHHCCCCEEEECCEECCCCCCCCCCCCCEEEHHHCCCHHHHHH
LINLLHQEQMHLVLDGVFSHVGKNSLYFNISGDYGDDEGAAKNPDSPYFDWFKFENYPFD
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCCC
YKSWWGIKDLPEIDKDNESFRDFIYGRKNSVLSKWNDLGIDGWRLDVADELPDSFIKGIR
HHCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCEEECCHHHCHHHHHHHHH
ENLDRYSEKILIGEIWEDASNKISYGTRRKYILGGSLQACMNYPFRDLIINLLNGNRSCQ
HHHHHHHHEEEEEHHHHCCCCCCCCCCCEEEEECCCHHHHHCCCHHHHHHHHHCCCCCHH
DVAHQLMTLQENYPKDIFYNNLNNLGTHDTERILTMVGKENLPIAVSLLFVLPGIPCIYY
HHHHHHHHHHHCCCHHHHHCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEE
GDEAGLSGGKDPENRKYFPWDDISPEIYDYYKSWTKKRLLENSLKEGEFSSFYSDRLLGI
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
LRYTDSEVFVEVINPSEDEIKIDYSEITFLQKFDFMPKLKDLLTEQVISGKSNIDLKIER
EEECCCEEEEEEECCCCCCEEEEHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEEC
>Mature Secondary Structure
MYYYNPWNLDYKQPFGAVKVGQAILLKFDANQTQVEVKCIIRRDFGKRYEFLMSRDESGF
CCEECCCCCCCCCCCCCEECCCEEEEEECCCCCEEEEEEEEEHHHHHHHHHHHCCCCCCE
FTVTIPFDENAGLYFYYFEILEGSDWEQNKRFYGSSGIGGEGLLYTNENDIKPYQLTVFE
EEEEEECCCCCCEEEEEEEEECCCCCCCCCCEECCCCCCCCEEEEECCCCCCCEEEEEEE
KEDKAPAWYRDAVFYQIFPDRFHNGNENGQVSHPKPNSFIYGTKSDTPFYVKEENGDIAR
CCCCCCCHHHHCEEEEECCHHHCCCCCCCEECCCCCCCEEEECCCCCCEEEEECCCCEEE
WDFFGGNLRGIINKIPYLKELGINAIYLNPIFSGTSNHRYDTNDYLKIDSMLGTQEDFQE
EEECCCCHHHHHHHCCHHHHCCCCEEEECCEECCCCCCCCCCCCCEEEHHHCCCHHHHHH
LINLLHQEQMHLVLDGVFSHVGKNSLYFNISGDYGDDEGAAKNPDSPYFDWFKFENYPFD
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCCC
YKSWWGIKDLPEIDKDNESFRDFIYGRKNSVLSKWNDLGIDGWRLDVADELPDSFIKGIR
HHCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCEEECCHHHCHHHHHHHHH
ENLDRYSEKILIGEIWEDASNKISYGTRRKYILGGSLQACMNYPFRDLIINLLNGNRSCQ
HHHHHHHHEEEEEHHHHCCCCCCCCCCCEEEEECCCHHHHHCCCHHHHHHHHHCCCCCHH
DVAHQLMTLQENYPKDIFYNNLNNLGTHDTERILTMVGKENLPIAVSLLFVLPGIPCIYY
HHHHHHHHHHHCCCHHHHHCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEE
GDEAGLSGGKDPENRKYFPWDDISPEIYDYYKSWTKKRLLENSLKEGEFSSFYSDRLLGI
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
LRYTDSEVFVEVINPSEDEIKIDYSEITFLQKFDFMPKLKDLLTEQVISGKSNIDLKIER
EEECCCEEEEEEECCCCCCEEEEHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2302196 [H]