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The map label for this gene is nusA [H]

Identifier: 125624585

GI number: 125624585

Start: 1774170

End: 1775318

Strand: Reverse

Name: nusA [H]

Synonym: llmg_1796

Alternate gene names: 125624585

Gene position: 1775318-1774170 (Counterclockwise)

Preceding gene: 125624586

Following gene: 125624584

Centisome position: 70.19

GC content: 38.64

Gene sequence:

>1149_bases
ATGAGCAAAGAAATGCTGAACGCCTTTGCAATGTTAGAAGAGCAAAAAGGAATTAAATCTGAGATTGTTATTGAAGCGAT
TGAGCAAGCTATCACAGCAGCTTACAAGCGTCAATATGGTCAAGCACAAAATGTTAAAGTTATCTTTGATGAAAAGAAAG
GAAACTTTAATGTTTATTCTACTCGTGAAGTAGTAGATGAAGTTTTTGATAGTCGTCTCGAAATTTCTCTTGAAGATGCT
CATGAATTAAATCCTCATTATGAAATTGGGGACAAAATCATGTTTGAAGAAAAACCAAAAGATTTTGCTCGTACTGCTGC
AGGGGCTGCTAAACAAGTCATCATGCAAAAAATGCGTGAAGAAGAACGCACAATCATTTACAACGAATATAGCCGTTACA
AGGACGAAATTATCCAAGGGACTGTTGAAAAAGTTGATGCACGTGCCGTTTATGTAAATCTTGGACGTGTTGATGCACTT
TTGACTAAGAAAGACCAAATTCCACAAGAAAACTTCCATGTGGGTGACCGTGTGAAGGTTTATGTGACTGATGTTGTCTT
GACTCCAAAAGGAACTCGTGTCTTTATTTCTCGTACTGCGCCTGATATGCTCAAACGCATGTTTGAAAAAGAAATTCCTG
AAGTTTTTGATGGAACTGTTGAAATCAAATCTATTGCTCGTGATGCTGGTGACCGTGCTAAGGTCATCGTTCAAAGTCAT
GATGAAAATGTTGATGCGATTGGTACAATGGTTGGGGCTAAAGGTTCACGTATTCAAGGAATTATTCGCGAACTTGCTGG
CGAAAAAATGGATATCATTGAATGGTCAGAAGACAAAGCAACTCTTGTAGCTAATGCTTTGAAACCTGCTCGTATTGAAC
AAGTTTTGATTACTGCTGATGGTTCAAGTCTGGCTGTTGTTGCTCGTGACCAATTGTCACTTGCCATTGGTAAACGCGGA
CAAAATGTTCGTCTTGCTGCTCACGTCACAAATAGCAAGATTGATATTAAAGCTGCTGACGAGTTTAACATGGACGATTA
TGAATTTGTTGGTGAAGCTGACGAAGAAACTCCAACTCTTGAAGTTGAGGAAACAATCTCAGTTGATGAAGTTGTTGAAG
AACCAGTAGTTAGCGAAAGTGAAGAATAA

Upstream 100 bases:

>100_bases
CTTGAGTTAGGTGAAATCGAAAAAATTTCCTTGATTCAAGGTAGTCGAACTTAGCAGATATTAAAAATGAAATTTTCTTA
TAAAAGAAAGAGAGAAAAAA

Downstream 100 bases:

>100_bases
ATTTTAAAAGTTTGAAAAATTTACTGACAGAATATAAAAGATTAGTAGAAGCATAAAGGTTCTAGGAATTTCTTTTTAAT
AAGTCTGTCAGTAAAATGAC

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MSKEMLNAFAMLEEQKGIKSEIVIEAIEQAITAAYKRQYGQAQNVKVIFDEKKGNFNVYSTREVVDEVFDSRLEISLEDA
HELNPHYEIGDKIMFEEKPKDFARTAAGAAKQVIMQKMREEERTIIYNEYSRYKDEIIQGTVEKVDARAVYVNLGRVDAL
LTKKDQIPQENFHVGDRVKVYVTDVVLTPKGTRVFISRTAPDMLKRMFEKEIPEVFDGTVEIKSIARDAGDRAKVIVQSH
DENVDAIGTMVGAKGSRIQGIIRELAGEKMDIIEWSEDKATLVANALKPARIEQVLITADGSSLAVVARDQLSLAIGKRG
QNVRLAAHVTNSKIDIKAADEFNMDDYEFVGEADEETPTLEVEETISVDEVVEEPVVSESEE

Sequences:

>Translated_382_residues
MSKEMLNAFAMLEEQKGIKSEIVIEAIEQAITAAYKRQYGQAQNVKVIFDEKKGNFNVYSTREVVDEVFDSRLEISLEDA
HELNPHYEIGDKIMFEEKPKDFARTAAGAAKQVIMQKMREEERTIIYNEYSRYKDEIIQGTVEKVDARAVYVNLGRVDAL
LTKKDQIPQENFHVGDRVKVYVTDVVLTPKGTRVFISRTAPDMLKRMFEKEIPEVFDGTVEIKSIARDAGDRAKVIVQSH
DENVDAIGTMVGAKGSRIQGIIRELAGEKMDIIEWSEDKATLVANALKPARIEQVLITADGSSLAVVARDQLSLAIGKRG
QNVRLAAHVTNSKIDIKAADEFNMDDYEFVGEADEETPTLEVEETISVDEVVEEPVVSESEE
>Mature_381_residues
SKEMLNAFAMLEEQKGIKSEIVIEAIEQAITAAYKRQYGQAQNVKVIFDEKKGNFNVYSTREVVDEVFDSRLEISLEDAH
ELNPHYEIGDKIMFEEKPKDFARTAAGAAKQVIMQKMREEERTIIYNEYSRYKDEIIQGTVEKVDARAVYVNLGRVDALL
TKKDQIPQENFHVGDRVKVYVTDVVLTPKGTRVFISRTAPDMLKRMFEKEIPEVFDGTVEIKSIARDAGDRAKVIVQSHD
ENVDAIGTMVGAKGSRIQGIIRELAGEKMDIIEWSEDKATLVANALKPARIEQVLITADGSSLAVVARDQLSLAIGKRGQ
NVRLAAHVTNSKIDIKAADEFNMDDYEFVGEADEETPTLEVEETISVDEVVEEPVVSESEE

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=379, Percent_Identity=34.0369393139842, Blast_Score=236, Evalue=2e-63,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 42894; Mature: 42763

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEMLNAFAMLEEQKGIKSEIVIEAIEQAITAAYKRQYGQAQNVKVIFDEKKGNFNVYS
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEE
TREVVDEVFDSRLEISLEDAHELNPHYEIGDKIMFEEKPKDFARTAAGAAKQVIMQKMRE
HHHHHHHHHCCCEEEEECHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHH
EERTIIYNEYSRYKDEIIQGTVEKVDARAVYVNLGRVDALLTKKDQIPQENFHVGDRVKV
HCCEEEEHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHCCCCCCCCCCCCCCEEEE
YVTDVVLTPKGTRVFISRTAPDMLKRMFEKEIPEVFDGTVEIKSIARDAGDRAKVIVQSH
EEEEEEECCCCCEEEEECCCHHHHHHHHHHHCHHHHCCCHHHHHHHHCCCCCEEEEEECC
DENVDAIGTMVGAKGSRIQGIIRELAGEKMDIIEWSEDKATLVANALKPARIEQVLITAD
CCCHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHCCCCCCEEEEEEEEC
GSSLAVVARDQLSLAIGKRGQNVRLAAHVTNSKIDIKAADEFNMDDYEFVGEADEETPTL
CCEEEEEEECCHHEEECCCCCCEEEEEEEECCEEEEEECCCCCCCCHHHCCCCCCCCCCE
EVEETISVDEVVEEPVVSESEE
EHHHCCCHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SKEMLNAFAMLEEQKGIKSEIVIEAIEQAITAAYKRQYGQAQNVKVIFDEKKGNFNVYS
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEE
TREVVDEVFDSRLEISLEDAHELNPHYEIGDKIMFEEKPKDFARTAAGAAKQVIMQKMRE
HHHHHHHHHCCCEEEEECHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHH
EERTIIYNEYSRYKDEIIQGTVEKVDARAVYVNLGRVDALLTKKDQIPQENFHVGDRVKV
HCCEEEEHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHCCCCCCCCCCCCCCEEEE
YVTDVVLTPKGTRVFISRTAPDMLKRMFEKEIPEVFDGTVEIKSIARDAGDRAKVIVQSH
EEEEEEECCCCCEEEEECCCHHHHHHHHHHHCHHHHCCCHHHHHHHHCCCCCEEEEEECC
DENVDAIGTMVGAKGSRIQGIIRELAGEKMDIIEWSEDKATLVANALKPARIEQVLITAD
CCCHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHCCCCCCEEEEEEEEC
GSSLAVVARDQLSLAIGKRGQNVRLAAHVTNSKIDIKAADEFNMDDYEFVGEADEETPTL
CCEEEEEEECCHHEEECCCCCCEEEEEEEECCEEEEEECCCCCCCCHHHCCCCCCCCCCE
EVEETISVDEVVEEPVVSESEE
EHHHCCCHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]