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The map label for this gene is metS [H]

Identifier: 125624554

GI number: 125624554

Start: 1748021

End: 1750009

Strand: Reverse

Name: metS [H]

Synonym: llmg_1764

Alternate gene names: 125624554

Gene position: 1750009-1748021 (Counterclockwise)

Preceding gene: 125624558

Following gene: 125624553

Centisome position: 69.18

GC content: 35.95

Gene sequence:

>1989_bases
ATGACAGAAAACAAAACTTTTTACATTACAACCCCAATTTATTATCCATCAGGCAAGTTGCATCTTGGTAGTTCTTACAC
AACTATTGCTTGCGACGTGCTTGCTCGTTACAAACGTTTGATGGGCTTTGATACTTTTTATTTAACAGGGCTTGATGAAC
ACGGAATGAAAATTCAACGAAAAGCCGAAGAATTGGGAATGACTCCTAAAGAATACTTGGACCCAATGGCGGCTGATGTT
CAAGAATTATGGAAAAAATTGGATATTTCTTATGATAAATTTATCCGTACTACAGACACTTATCATGAAGAAGCCGTAGC
GAAGGCTTTTGAACAGTTATTGGAACAAGATGATATTTATTTGGGAAAATATGCGGGTTGGTATTCTGTTTCTGATGAAG
AGTTTTTCACTGAAACACAACTTGAAGAAATTTTCCGAGATGAATCTGGAAATATTACTGGTGGCATTGCACCTTCTGGT
CATGAAGTGGAATGGGTTGAAGAAGAAACTTATTTCTTCCGCATGGGAAAATATGCTGACTGGCTTTTACAATATTATGA
TGAACATCCAGATTTTATTCAACCTGAGGTTCGTAAAAATGAAATGGTCAATAATTTCATCAAGCCAGGTCTAGAAGATT
TGGCTTTGACACGGACAAGTTTTACTTGGGGAATTCCTGTCCCTTCTAATCCTAAGCATGTGGTCTACGTGTGGTTTGAT
GCTTTATTGAACTATATTACAGCGCTCGGCTATAATTCTGACAATGACAGTAATTTTAAAAAATACTGGCCTGGAATTAA
TATGGTTGGTAAAGAAATTGTTCGTTTCCATACGATTTACTGGCCAATTATGCTTCATGCTCTAGGTTTACCTGCTCCTA
AAAAGATTTTCGGACATGGATGGCTCTTGATGAAAGACGGAAAAATGTCTAAATCAAAAGGAAATGTTGTTTATCCTGAG
ATGTTGATTGAACGTTATGGCCTTGATGCGGTTCGTTATTATTTGATGCGTGCGATTTCTTTTGGACAAGATGGAATTTT
CACTCCAGAAGACTTTGTTGGACGTATTAATTTTGATTTGGCCAATGATTTGGGGAATTTGTTGAATCGTACCGTTTCAA
TGATTAACAAATATAATGACGGAAAAATCGAAGCAACTGGAGTTTCGACTGAATTTGATGCAAGTTTGGAAGAAGTGGTT
GAAGAAACAATCAGTCATTTCCATAAAGCAATGGATAAATTTGAATTCAACGTAGCTTTGGCTGATGTTTGGACATTGAT
TTCAAGAACAAATAAATATATTGATGAAACAGCACCTTGGGTCCTTGCAAAATCAGAAGACGACAAAGCAAAATTGAATA
ATGTTTTGTATCATTTGGCAGAAAATTTACGGATTGCAGGTGCACTTTTACAACCATTTATGCGTGCAACATCAGGTAAA
ATCTTTGAGCAATTGGGAATGGATGAAAGATCATTTTCACTTGAAAATCTTTCTTTTGGCTATTCATTTACTCATCCTGT
AGTAGCAAAAGGTCAACCTATTTTCCCACGTCTTGATGTGGAAGAAGAGGTTGCTTATATTAAATTACAAATGGCTGGTG
GCGTATTGCCAGAAAAAGAGTGGGTCCCTGAAGAAGTTGAATTGAATTTAACTTTACCACAAATTAAATTTGATGATTTT
GAAAAAATTGAACTAAAAGTTGCCGAAGTGCTTGAAGTAGAACCTGTTGAAGGTTCTGATAAATTGTTGAGATTTAAATT
GGATGCTGGTGATTCTGAACCACGTCAAATCTTGTCAGGAATTGCACAATTCTATCCAAATGAACAAGAATTAGTAGGTA
AAAAATTACAAATCGTAGCCAATTTGAAACCACGTAAAATGATGAAAAAATACGTTTCACAAGGAATGATTTTGTCAGCC
GAATTTGATGGAAAATTGCGTGTCTTGACCGTTGATGATGATGTTCCTGCTGGTTCATTGATTGGGTAA

Upstream 100 bases:

>100_bases
AAACTTAATCAGACAATGCGATTTTAGAGGTTTTTTGGTATAATATTTAGATGAGAATCTTCTCAAATATCGAGCCTCAT
TTGCTCGGAAAGAGGAAAAT

Downstream 100 bases:

>100_bases
AATTAATTAGACAATAAAAAATGTGAAAGATTTGTTATCTTTCACGTTTTTTTACGTATAGTATATCAGGAGAGAAATCT
CTTAGTGTTCACTTGATTTC

Product: MetS protein

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 662; Mature: 661

Protein sequence:

>662_residues
MTENKTFYITTPIYYPSGKLHLGSSYTTIACDVLARYKRLMGFDTFYLTGLDEHGMKIQRKAEELGMTPKEYLDPMAADV
QELWKKLDISYDKFIRTTDTYHEEAVAKAFEQLLEQDDIYLGKYAGWYSVSDEEFFTETQLEEIFRDESGNITGGIAPSG
HEVEWVEEETYFFRMGKYADWLLQYYDEHPDFIQPEVRKNEMVNNFIKPGLEDLALTRTSFTWGIPVPSNPKHVVYVWFD
ALLNYITALGYNSDNDSNFKKYWPGINMVGKEIVRFHTIYWPIMLHALGLPAPKKIFGHGWLLMKDGKMSKSKGNVVYPE
MLIERYGLDAVRYYLMRAISFGQDGIFTPEDFVGRINFDLANDLGNLLNRTVSMINKYNDGKIEATGVSTEFDASLEEVV
EETISHFHKAMDKFEFNVALADVWTLISRTNKYIDETAPWVLAKSEDDKAKLNNVLYHLAENLRIAGALLQPFMRATSGK
IFEQLGMDERSFSLENLSFGYSFTHPVVAKGQPIFPRLDVEEEVAYIKLQMAGGVLPEKEWVPEEVELNLTLPQIKFDDF
EKIELKVAEVLEVEPVEGSDKLLRFKLDAGDSEPRQILSGIAQFYPNEQELVGKKLQIVANLKPRKMMKKYVSQGMILSA
EFDGKLRVLTVDDDVPAGSLIG

Sequences:

>Translated_662_residues
MTENKTFYITTPIYYPSGKLHLGSSYTTIACDVLARYKRLMGFDTFYLTGLDEHGMKIQRKAEELGMTPKEYLDPMAADV
QELWKKLDISYDKFIRTTDTYHEEAVAKAFEQLLEQDDIYLGKYAGWYSVSDEEFFTETQLEEIFRDESGNITGGIAPSG
HEVEWVEEETYFFRMGKYADWLLQYYDEHPDFIQPEVRKNEMVNNFIKPGLEDLALTRTSFTWGIPVPSNPKHVVYVWFD
ALLNYITALGYNSDNDSNFKKYWPGINMVGKEIVRFHTIYWPIMLHALGLPAPKKIFGHGWLLMKDGKMSKSKGNVVYPE
MLIERYGLDAVRYYLMRAISFGQDGIFTPEDFVGRINFDLANDLGNLLNRTVSMINKYNDGKIEATGVSTEFDASLEEVV
EETISHFHKAMDKFEFNVALADVWTLISRTNKYIDETAPWVLAKSEDDKAKLNNVLYHLAENLRIAGALLQPFMRATSGK
IFEQLGMDERSFSLENLSFGYSFTHPVVAKGQPIFPRLDVEEEVAYIKLQMAGGVLPEKEWVPEEVELNLTLPQIKFDDF
EKIELKVAEVLEVEPVEGSDKLLRFKLDAGDSEPRQILSGIAQFYPNEQELVGKKLQIVANLKPRKMMKKYVSQGMILSA
EFDGKLRVLTVDDDVPAGSLIG
>Mature_661_residues
TENKTFYITTPIYYPSGKLHLGSSYTTIACDVLARYKRLMGFDTFYLTGLDEHGMKIQRKAEELGMTPKEYLDPMAADVQ
ELWKKLDISYDKFIRTTDTYHEEAVAKAFEQLLEQDDIYLGKYAGWYSVSDEEFFTETQLEEIFRDESGNITGGIAPSGH
EVEWVEEETYFFRMGKYADWLLQYYDEHPDFIQPEVRKNEMVNNFIKPGLEDLALTRTSFTWGIPVPSNPKHVVYVWFDA
LLNYITALGYNSDNDSNFKKYWPGINMVGKEIVRFHTIYWPIMLHALGLPAPKKIFGHGWLLMKDGKMSKSKGNVVYPEM
LIERYGLDAVRYYLMRAISFGQDGIFTPEDFVGRINFDLANDLGNLLNRTVSMINKYNDGKIEATGVSTEFDASLEEVVE
ETISHFHKAMDKFEFNVALADVWTLISRTNKYIDETAPWVLAKSEDDKAKLNNVLYHLAENLRIAGALLQPFMRATSGKI
FEQLGMDERSFSLENLSFGYSFTHPVVAKGQPIFPRLDVEEEVAYIKLQMAGGVLPEKEWVPEEVELNLTLPQIKFDDFE
KIELKVAEVLEVEPVEGSDKLLRFKLDAGDSEPRQILSGIAQFYPNEQELVGKKLQIVANLKPRKMMKKYVSQGMILSAE
FDGKLRVLTVDDDVPAGSLIG

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=543, Percent_Identity=34.4383057090239, Blast_Score=296, Evalue=4e-80,
Organism=Homo sapiens, GI14043022, Length=575, Percent_Identity=25.3913043478261, Blast_Score=160, Evalue=4e-39,
Organism=Homo sapiens, GI4507947, Length=132, Percent_Identity=37.1212121212121, Blast_Score=67, Evalue=6e-11,
Organism=Escherichia coli, GI1788432, Length=673, Percent_Identity=28.6775631500743, Blast_Score=251, Evalue=1e-67,
Organism=Caenorhabditis elegans, GI212645096, Length=503, Percent_Identity=32.8031809145129, Blast_Score=276, Evalue=4e-74,
Organism=Caenorhabditis elegans, GI71992781, Length=395, Percent_Identity=32.1518987341772, Blast_Score=200, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI17540794, Length=579, Percent_Identity=25.73402417962, Blast_Score=156, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6321610, Length=549, Percent_Identity=31.6939890710383, Blast_Score=270, Evalue=5e-73,
Organism=Saccharomyces cerevisiae, GI6321704, Length=480, Percent_Identity=25, Blast_Score=117, Evalue=6e-27,
Organism=Drosophila melanogaster, GI21358483, Length=504, Percent_Identity=31.7460317460317, Blast_Score=269, Evalue=5e-72,
Organism=Drosophila melanogaster, GI24655311, Length=556, Percent_Identity=24.6402877697842, Blast_Score=152, Evalue=5e-37,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 75833; Mature: 75702

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTENKTFYITTPIYYPSGKLHLGSSYTTIACDVLARYKRLMGFDTFYLTGLDEHGMKIQR
CCCCCEEEEECCEEECCCCEEECCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHH
KAEELGMTPKEYLDPMAADVQELWKKLDISYDKFIRTTDTYHEEAVAKAFEQLLEQDDIY
HHHHHCCCHHHHHCHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCEE
LGKYAGWYSVSDEEFFTETQLEEIFRDESGNITGGIAPSGHEVEWVEEETYFFRMGKYAD
EECCCCEEECCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCEEEEECCHHEEEHHHHHH
WLLQYYDEHPDFIQPEVRKNEMVNNFIKPGLEDLALTRTSFTWGIPVPSNPKHVVYVWFD
HHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHEEECCEEECCCCCCCCCEEEEEHHH
ALLNYITALGYNSDNDSNFKKYWPGINMVGKEIVRFHTIYWPIMLHALGLPAPKKIFGHG
HHHHHHHHHCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCC
WLLMKDGKMSKSKGNVVYPEMLIERYGLDAVRYYLMRAISFGQDGIFTPEDFVGRINFDL
EEEEECCCCCCCCCCEECHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCHHHHEEECHHH
ANDLGNLLNRTVSMINKYNDGKIEATGVSTEFDASLEEVVEETISHFHKAMDKFEFNVAL
HHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADVWTLISRTNKYIDETAPWVLAKSEDDKAKLNNVLYHLAENLRIAGALLQPFMRATSGK
HHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFEQLGMDERSFSLENLSFGYSFTHPVVAKGQPIFPRLDVEEEVAYIKLQMAGGVLPEKE
HHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCHHCEEEEEEEECCCCCCCCC
WVPEEVELNLTLPQIKFDDFEKIELKVAEVLEVEPVEGSDKLLRFKLDAGDSEPRQILSG
CCCCCEEEEEEECCCCCCCCHHHHHHHHHHHEECCCCCCCCEEEEEECCCCCCHHHHHHH
IAQFYPNEQELVGKKLQIVANLKPRKMMKKYVSQGMILSAEFDGKLRVLTVDDDVPAGSL
HHHHCCCHHHHHCCHHHEEECCCHHHHHHHHHHCCEEEEEECCCEEEEEEECCCCCCCCC
IG
CC
>Mature Secondary Structure 
TENKTFYITTPIYYPSGKLHLGSSYTTIACDVLARYKRLMGFDTFYLTGLDEHGMKIQR
CCCCEEEEECCEEECCCCEEECCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHH
KAEELGMTPKEYLDPMAADVQELWKKLDISYDKFIRTTDTYHEEAVAKAFEQLLEQDDIY
HHHHHCCCHHHHHCHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCEE
LGKYAGWYSVSDEEFFTETQLEEIFRDESGNITGGIAPSGHEVEWVEEETYFFRMGKYAD
EECCCCEEECCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCEEEEECCHHEEEHHHHHH
WLLQYYDEHPDFIQPEVRKNEMVNNFIKPGLEDLALTRTSFTWGIPVPSNPKHVVYVWFD
HHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHEEECCEEECCCCCCCCCEEEEEHHH
ALLNYITALGYNSDNDSNFKKYWPGINMVGKEIVRFHTIYWPIMLHALGLPAPKKIFGHG
HHHHHHHHHCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCC
WLLMKDGKMSKSKGNVVYPEMLIERYGLDAVRYYLMRAISFGQDGIFTPEDFVGRINFDL
EEEEECCCCCCCCCCEECHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCHHHHEEECHHH
ANDLGNLLNRTVSMINKYNDGKIEATGVSTEFDASLEEVVEETISHFHKAMDKFEFNVAL
HHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADVWTLISRTNKYIDETAPWVLAKSEDDKAKLNNVLYHLAENLRIAGALLQPFMRATSGK
HHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFEQLGMDERSFSLENLSFGYSFTHPVVAKGQPIFPRLDVEEEVAYIKLQMAGGVLPEKE
HHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCHHCEEEEEEEECCCCCCCCC
WVPEEVELNLTLPQIKFDDFEKIELKVAEVLEVEPVEGSDKLLRFKLDAGDSEPRQILSG
CCCCCEEEEEEECCCCCCCCHHHHHHHHHHHEECCCCCCCCEEEEEECCCCCCHHHHHHH
IAQFYPNEQELVGKKLQIVANLKPRKMMKKYVSQGMILSAEFDGKLRVLTVDDDVPAGSL
HHHHCCCHHHHHCCHHHEEECCCHHHHHHHHHHCCEEEEEECCCEEEEEEECCCCCCCCC
IG
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]