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The map label for this gene is mraW [H]

Identifier: 125624479

GI number: 125624479

Start: 1656140

End: 1657093

Strand: Reverse

Name: mraW [H]

Synonym: llmg_1681

Alternate gene names: 125624479

Gene position: 1657093-1656140 (Counterclockwise)

Preceding gene: 125624480

Following gene: 125624478

Centisome position: 65.51

GC content: 36.27

Gene sequence:

>954_bases
TTGCCAAAAACAATAGCATTTAAGCACGATACAGTCCTATTACATGAGACTGTGGACATGCTTGAAGTCAAGCCAAATGG
AATTTATGTTGATGCGACACTAGGTGGCGCAGGACATAGTGAGTATCTTCTTAGTAAACTAACTACTGGTCATCTTTATT
CATTTGACCAAGATGAGACCGCTCATGCAAACGCAAAAGTACGTTTGAGTGAGCAACTTGCAGAAGATAAAGTAACATTG
ATAAAAAGTAATTTTCGTAATCTAAAATCGGCGTTAGCTGAATTAGACATAACGAAAATCGACGGAATTCTTTATGATTT
AGGTGTTTCTAGCCCCCAATTCGATGATAGTCAGCGTGGTTTCTCATATAAAAAAGAAGCTCGATTAGATATGCGAATGG
ATCAAAGTCAAACACTTTCTGCCTATGAAGTTGTAAATGATTATCCTTATGAAGCATTGGTTCGAATTTTTTTCCGCTAT
GGCGAAGATAAATTTTCTAAACAGATTGCTCGTAAGATTGAACAAAGTAGAAAAATTAAACCAATTGAGACAACAATTGA
GCTTGCAGATTTGATTAAATCGGCACTACCTCAAAAAGAACTCAAGAAAAAAGGGCATCCAGCCAAACGTATATTCCAAG
CTATTCGTATAGAAGTAAATGACGAGCTTGGGGCGGCAGAAGAATCAATTGAACAGGCCATTGACCTATTAAAAGTTGAT
GGACGAATTTCGGTAATCACTTTTCATTCTTTAGAAGATCGTCTGACAAAATCTATATTCAAAGAATATAGTACAGTCAA
TGTTCCTAAAGGATTACCGATGCTTCCTAAAGAAATGGAAGCAAAACTAAAATTGATTAATCGAAAGCCAGTGCTCGCTA
GTGAAGAAGAACTGGAATTTAATAATCGAGCTCATAGTGCAAAATTACGAGTCGCACAAAAGCAAAGAGATTAA

Upstream 100 bases:

>100_bases
GCGACTTCTTAAAAAAATCTTTACATAATCATGTAAACTACTACAAAAATGGGAAAAAAGTGATATAATAAATTGTAAGT
ATACAAGGAGGACGGCGCTC

Downstream 100 bases:

>100_bases
TTAAGAAATATGTTGGTCTAATAAACGGGTTTTTCCCACTTGAGGATTGATTTTGGATGTCTTATATAAGTGAATAATAT
TGTTCAGTTTCTCCAATTTT

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH [H]

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MPKTIAFKHDTVLLHETVDMLEVKPNGIYVDATLGGAGHSEYLLSKLTTGHLYSFDQDETAHANAKVRLSEQLAEDKVTL
IKSNFRNLKSALAELDITKIDGILYDLGVSSPQFDDSQRGFSYKKEARLDMRMDQSQTLSAYEVVNDYPYEALVRIFFRY
GEDKFSKQIARKIEQSRKIKPIETTIELADLIKSALPQKELKKKGHPAKRIFQAIRIEVNDELGAAEESIEQAIDLLKVD
GRISVITFHSLEDRLTKSIFKEYSTVNVPKGLPMLPKEMEAKLKLINRKPVLASEEELEFNNRAHSAKLRVAQKQRD

Sequences:

>Translated_317_residues
MPKTIAFKHDTVLLHETVDMLEVKPNGIYVDATLGGAGHSEYLLSKLTTGHLYSFDQDETAHANAKVRLSEQLAEDKVTL
IKSNFRNLKSALAELDITKIDGILYDLGVSSPQFDDSQRGFSYKKEARLDMRMDQSQTLSAYEVVNDYPYEALVRIFFRY
GEDKFSKQIARKIEQSRKIKPIETTIELADLIKSALPQKELKKKGHPAKRIFQAIRIEVNDELGAAEESIEQAIDLLKVD
GRISVITFHSLEDRLTKSIFKEYSTVNVPKGLPMLPKEMEAKLKLINRKPVLASEEELEFNNRAHSAKLRVAQKQRD
>Mature_316_residues
PKTIAFKHDTVLLHETVDMLEVKPNGIYVDATLGGAGHSEYLLSKLTTGHLYSFDQDETAHANAKVRLSEQLAEDKVTLI
KSNFRNLKSALAELDITKIDGILYDLGVSSPQFDDSQRGFSYKKEARLDMRMDQSQTLSAYEVVNDYPYEALVRIFFRYG
EDKFSKQIARKIEQSRKIKPIETTIELADLIKSALPQKELKKKGHPAKRIFQAIRIEVNDELGAAEESIEQAIDLLKVDG
RISVITFHSLEDRLTKSIFKEYSTVNVPKGLPMLPKEMEAKLKLINRKPVLASEEELEFNNRAHSAKLRVAQKQRD

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA [H]

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family [H]

Homologues:

Organism=Homo sapiens, GI165377209, Length=343, Percent_Identity=33.8192419825073, Blast_Score=158, Evalue=7e-39,
Organism=Homo sapiens, GI165377202, Length=195, Percent_Identity=37.9487179487179, Blast_Score=113, Evalue=3e-25,
Organism=Escherichia coli, GI1786270, Length=316, Percent_Identity=41.7721518987342, Blast_Score=230, Evalue=7e-62,
Organism=Drosophila melanogaster, GI62472493, Length=328, Percent_Identity=34.1463414634146, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI28571637, Length=328, Percent_Identity=34.1463414634146, Blast_Score=172, Evalue=3e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002903 [H]

Pfam domain/function: PF01795 Methyltransf_5 [H]

EC number: NA

Molecular weight: Translated: 36051; Mature: 35920

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKTIAFKHDTVLLHETVDMLEVKPNGIYVDATLGGAGHSEYLLSKLTTGHLYSFDQDET
CCCCEEECCCEEEEECHHHHEEECCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCC
AHANAKVRLSEQLAEDKVTLIKSNFRNLKSALAELDITKIDGILYDLGVSSPQFDDSQRG
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHEECCCCCCCCCCCCCC
FSYKKEARLDMRMDQSQTLSAYEVVNDYPYEALVRIFFRYGEDKFSKQIARKIEQSRKIK
CCCHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC
PIETTIELADLIKSALPQKELKKKGHPAKRIFQAIRIEVNDELGAAEESIEQAIDLLKVD
CHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHCC
GRISVITFHSLEDRLTKSIFKEYSTVNVPKGLPMLPKEMEAKLKLINRKPVLASEEELEF
CEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHH
NNRAHSAKLRVAQKQRD
CCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
PKTIAFKHDTVLLHETVDMLEVKPNGIYVDATLGGAGHSEYLLSKLTTGHLYSFDQDET
CCCEEECCCEEEEECHHHHEEECCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCC
AHANAKVRLSEQLAEDKVTLIKSNFRNLKSALAELDITKIDGILYDLGVSSPQFDDSQRG
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHEECCCCCCCCCCCCCC
FSYKKEARLDMRMDQSQTLSAYEVVNDYPYEALVRIFFRYGEDKFSKQIARKIEQSRKIK
CCCHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC
PIETTIELADLIKSALPQKELKKKGHPAKRIFQAIRIEVNDELGAAEESIEQAIDLLKVD
CHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHCC
GRISVITFHSLEDRLTKSIFKEYSTVNVPKGLPMLPKEMEAKLKLINRKPVLASEEELEF
CEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHH
NNRAHSAKLRVAQKQRD
CCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]