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The map label for this gene is folC [H]

Identifier: 125624154

GI number: 125624154

Start: 1310463

End: 1311746

Strand: Reverse

Name: folC [H]

Synonym: llmg_1334

Alternate gene names: 125624154

Gene position: 1311746-1310463 (Counterclockwise)

Preceding gene: 125624155

Following gene: 125624153

Centisome position: 51.86

GC content: 36.06

Gene sequence:

>1284_bases
ATGTCTATTGAACAAGCATTAGAATGGATACATTCACGATTAAAATTTAATATTCGCCCAGGGCTAAATCGAGTTTCAGC
ACTTTTAGAATTATTAGGCAATCCAGAGGAATCTTTATCAATGATTCACATTGCTGGAACAAATGGGAAAGGATCAACTG
TTGCCTTTACGCGCTCTATTTTTATGCAAGCTGGCTTAAAAGTTGCTTCTTTTACGAGTCCTTTTATTACGACTTTTGGT
GAACGAATGTCTATAAATGCTCAACCAATTCCTGATGAAAAGTTAATTTATTATGTAAAGATGATTCAACCTTTAGTTGA
GCAGCTTGATAATGATGCTGAGTTGACTGGAATTACTGAATTTGAAATTATTACCGCGATGGCTTTTAAATATTTTGCTG
ATGAAAAGGTTGATTTGGCGGTAATCGAGGTTGGCTTGGGTGGACTGCTTGATTCGACAAATGTCATTAGACCCGTTGTT
TCTGGAATCACAACAATCGGGCTAGACCATATTGATATCTTGGGTTCAACGATTGAAGAAATCGCAGCTCAAAAAGCAGG
TATTATTAAAGCTGGAATACCTGTTGTGGTTGGAAACATTGAATTAAAAGCTTTGCGAGTTATTTGGGAAGTTGCCAGAA
AAAATACTGCGCGTATTTATCAGTATCCTTATGATTACCGCACCGAGTTAAAAGAGCATGAACATTTTGATTTCTTTGCA
GGTCAAGAGGCAATTCTGGATTTGGAAAAATCTTTGATTGGCTTGCATCAAGTAGAAAATGCTGGAATGGCCATTGAATT
GGCGCTCGTCTATGCGAATAAAGTTGGAATTAACTTGAATGAAGAGATTATTCGTGCGGGAATTCGTGAAGCTTTTTGGC
CTGCTCGAATGGAAAAATTAGGCGAAAATCCTTTAATTTTACTTGATGGGGCTCATAATGTTCATGCTATGAATCGTTTG
CTTGAGAACTTGGGGAAAGAATTCCCTGATAAAAAAATTACAATTATTTTTTCAGCTATTACCACAAAAGATATTAGGCA
GATGATAAAAATGCTTCAAACGGTAAAAAATTCTCGCTTAATTTTGACAACTTTTGATTATCCTAAAGCTTTAGACTTGA
AAGATTTTCGGTATTTAGAGGATGAAGGGGTTGAATTAGCACCAAGTTGGGAATTAGCGATTGTTCGTGCACAAAAAGCT
TTAGATGAAGATGAGCTCCTATTAATCACTGGTTCGCTCTATTTTTCATCACAAGTTCGTGAATTTTTGAAAAAAGAAAA
GTAG

Upstream 100 bases:

>100_bases
GCTTGCTGAGCTACCTTTTTTTCTCCTTGAACTAGAAGAAGCACCAGAAGAGTTTAAAAAGTATTATCGTTATAAGAACA
TATAATAGAAAAGGTCAAAA

Downstream 100 bases:

>100_bases
TAAATAGAAGTAGTTTTCGTTTCTAAAATTCATTAAATAGTTGTAAATCTTAAAAATTCGGCTATAATAAAATTGATTTA
ATAAATTCATTCTTTATTTG

Product: FolC protein

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MSIEQALEWIHSRLKFNIRPGLNRVSALLELLGNPEESLSMIHIAGTNGKGSTVAFTRSIFMQAGLKVASFTSPFITTFG
ERMSINAQPIPDEKLIYYVKMIQPLVEQLDNDAELTGITEFEIITAMAFKYFADEKVDLAVIEVGLGGLLDSTNVIRPVV
SGITTIGLDHIDILGSTIEEIAAQKAGIIKAGIPVVVGNIELKALRVIWEVARKNTARIYQYPYDYRTELKEHEHFDFFA
GQEAILDLEKSLIGLHQVENAGMAIELALVYANKVGINLNEEIIRAGIREAFWPARMEKLGENPLILLDGAHNVHAMNRL
LENLGKEFPDKKITIIFSAITTKDIRQMIKMLQTVKNSRLILTTFDYPKALDLKDFRYLEDEGVELAPSWELAIVRAQKA
LDEDELLLITGSLYFSSQVREFLKKEK

Sequences:

>Translated_427_residues
MSIEQALEWIHSRLKFNIRPGLNRVSALLELLGNPEESLSMIHIAGTNGKGSTVAFTRSIFMQAGLKVASFTSPFITTFG
ERMSINAQPIPDEKLIYYVKMIQPLVEQLDNDAELTGITEFEIITAMAFKYFADEKVDLAVIEVGLGGLLDSTNVIRPVV
SGITTIGLDHIDILGSTIEEIAAQKAGIIKAGIPVVVGNIELKALRVIWEVARKNTARIYQYPYDYRTELKEHEHFDFFA
GQEAILDLEKSLIGLHQVENAGMAIELALVYANKVGINLNEEIIRAGIREAFWPARMEKLGENPLILLDGAHNVHAMNRL
LENLGKEFPDKKITIIFSAITTKDIRQMIKMLQTVKNSRLILTTFDYPKALDLKDFRYLEDEGVELAPSWELAIVRAQKA
LDEDELLLITGSLYFSSQVREFLKKEK
>Mature_426_residues
SIEQALEWIHSRLKFNIRPGLNRVSALLELLGNPEESLSMIHIAGTNGKGSTVAFTRSIFMQAGLKVASFTSPFITTFGE
RMSINAQPIPDEKLIYYVKMIQPLVEQLDNDAELTGITEFEIITAMAFKYFADEKVDLAVIEVGLGGLLDSTNVIRPVVS
GITTIGLDHIDILGSTIEEIAAQKAGIIKAGIPVVVGNIELKALRVIWEVARKNTARIYQYPYDYRTELKEHEHFDFFAG
QEAILDLEKSLIGLHQVENAGMAIELALVYANKVGINLNEEIIRAGIREAFWPARMEKLGENPLILLDGAHNVHAMNRLL
ENLGKEFPDKKITIIFSAITTKDIRQMIKMLQTVKNSRLILTTFDYPKALDLKDFRYLEDEGVELAPSWELAIVRAQKAL
DEDELLLITGSLYFSSQVREFLKKEK

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=363, Percent_Identity=32.5068870523416, Blast_Score=179, Evalue=6e-45,
Organism=Homo sapiens, GI66932990, Length=363, Percent_Identity=32.5068870523416, Blast_Score=178, Evalue=7e-45,
Organism=Escherichia coli, GI1788654, Length=426, Percent_Identity=28.8732394366197, Blast_Score=149, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI71984923, Length=358, Percent_Identity=27.0949720670391, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17553150, Length=362, Percent_Identity=27.9005524861878, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17553148, Length=362, Percent_Identity=27.9005524861878, Blast_Score=140, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6323760, Length=426, Percent_Identity=30.7511737089202, Blast_Score=181, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6324815, Length=303, Percent_Identity=31.6831683168317, Blast_Score=127, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6322718, Length=180, Percent_Identity=33.8888888888889, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24641571, Length=294, Percent_Identity=33.6734693877551, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24581568, Length=193, Percent_Identity=28.4974093264249, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 47934; Mature: 47803

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00589 PTS_HPR_SER ; PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIEQALEWIHSRLKFNIRPGLNRVSALLELLGNPEESLSMIHIAGTNGKGSTVAFTRSI
CCHHHHHHHHHHHHEEECCCCHHHHHHHHHHHCCCCHHEEEEEEEECCCCCCCHHHHHHH
FMQAGLKVASFTSPFITTFGERMSINAQPIPDEKLIYYVKMIQPLVEQLDNDAELTGITE
HHHCCCCHHHCCCHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHH
FEIITAMAFKYFADEKVDLAVIEVGLGGLLDSTNVIRPVVSGITTIGLDHIDILGSTIEE
HHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
IAAQKAGIIKAGIPVVVGNIELKALRVIWEVARKNTARIYQYPYDYRTELKEHEHFDFFA
HHHHHCCCEECCCCEEEECCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHCCCCCCHHC
GQEAILDLEKSLIGLHQVENAGMAIELALVYANKVGINLNEEIIRAGIREAFWPARMEKL
CHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHCHHHHHHC
GENPLILLDGAHNVHAMNRLLENLGKEFPDKKITIIFSAITTKDIRQMIKMLQTVKNSRL
CCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHCCCEE
ILTTFDYPKALDLKDFRYLEDEGVELAPSWELAIVRAQKALDEDELLLITGSLYFSSQVR
EEEECCCCCCCCCHHHHHHCCCCCEECCCCCEEEEEHHHCCCCCCEEEEECCHHHHHHHH
EFLKKEK
HHHHHCC
>Mature Secondary Structure 
SIEQALEWIHSRLKFNIRPGLNRVSALLELLGNPEESLSMIHIAGTNGKGSTVAFTRSI
CHHHHHHHHHHHHEEECCCCHHHHHHHHHHHCCCCHHEEEEEEEECCCCCCCHHHHHHH
FMQAGLKVASFTSPFITTFGERMSINAQPIPDEKLIYYVKMIQPLVEQLDNDAELTGITE
HHHCCCCHHHCCCHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHH
FEIITAMAFKYFADEKVDLAVIEVGLGGLLDSTNVIRPVVSGITTIGLDHIDILGSTIEE
HHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
IAAQKAGIIKAGIPVVVGNIELKALRVIWEVARKNTARIYQYPYDYRTELKEHEHFDFFA
HHHHHCCCEECCCCEEEECCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHCCCCCCHHC
GQEAILDLEKSLIGLHQVENAGMAIELALVYANKVGINLNEEIIRAGIREAFWPARMEKL
CHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHCHHHHHHC
GENPLILLDGAHNVHAMNRLLENLGKEFPDKKITIIFSAITTKDIRQMIKMLQTVKNSRL
CCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHCCCEE
ILTTFDYPKALDLKDFRYLEDEGVELAPSWELAIVRAQKALDEDELLLITGSLYFSSQVR
EEEECCCCCCCCCHHHHHHCCCCCEECCCCCEEEEEHHHCCCCCCEEEEECCHHHHHHHH
EFLKKEK
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]