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The map label for this gene is als [H]

Identifier: 125624130

GI number: 125624130

Start: 1279940

End: 1281604

Strand: Reverse

Name: als [H]

Synonym: llmg_1309

Alternate gene names: 125624130

Gene position: 1281604-1279940 (Counterclockwise)

Preceding gene: 125624131

Following gene: 125624129

Centisome position: 50.67

GC content: 41.8

Gene sequence:

>1665_bases
ATGTCTGAGAAACAATTTGGGGCGAACTTGGTTGTCGATAGTTTGATTAACCATAAAGTGAAGTATGTATTTGGGATTCC
AGGAGCAAAAATTGACCGGGTTTTTGATTTATTAGAAAATGAAGAAGGCCCTCAAATGGTCGTGACTCGTCATGAGCAAG
GAGCTGCTTTCATGGCTCAAGCAGTTGGCCGTTTGACTGGCGAACCTGGTGTAGTAGTTGTTACGAGTGGTCCAGGGGTA
TCAAACCTTGCAACTCCACTTTTGACTGCAACATCAGAAGGTGACGCTATTTTAGCCATTGGTGGACAAGTTAAACGCAG
TGACCGTCTCAAACGTGCACACCAATCAATGGATAATGCCGGAATGATGCAATCAGCAACAAAATATTCAGCAGAAGTAC
TTGATCCTGATACACTTTCTGAATCAATTGCCAATGCCTATCGTATTGCCAAATCAGGACATCCAGGTGCAACTTTCTTG
TCAATCCCCCAAGATGTAACGGATGCCGAAGTATCAATCAAAGCCATTCAACCACTTTCAGACCCTAAAATGGGGAATGC
CTCTATTGATGACATCAATTACCTAGCGCAAGCCATCAAAAATGCCGTTTTGCCAGTAATTTTGGTTGGAGCTGGAGCTT
CAGATGTCAAAGTCGCTTCATCATTGCGTAACTTATTGACTCATGTTAATATTCCTGTCGTTGAAACATTCCAAGGTGCT
GGTGTTATTTCACATGATTTAGAGCATACTTTTTACGGACGTATCGGTCTTTTCCGCAACCAACCAGGGGATATGCTTTT
GAAACGCTCTGACCTTGTTATTGCTGTTGGTTACGACCCAATCGAATACGAAGCTCGCAACTGGAATGCCGAAATTGATA
GCCGCATCATCGTTATTGATAATGCCATTGCTGAAATTGATACTTACTACCAACCAGAACGTGAATTAATTGGGGACATC
GCGGCAACATTGGATAATCTTTTACCAGCCGTTCGTGGTTATAAAATTCCAAAAGGAACAAAAGAATATCTTGATGGCCT
TCATGAAGTTGCTGAACAACATGAATTCGATACTGAAAACACCGAAGAAGGCAGAATGCACCCACTTGATTTGGTCAGCA
CTTTCCAAGAAATCGTCAAAGATGATGAAACAGTCACAGTTGACGTAGGTTCACTATACATTTGGATGGCACGTCATTTC
AAATCATACGAACCACGTCACCTTCTATTCTCAAACGGAATGCAAACACTTGGTGTTGCACTCCCTTGGGCAATCACAGC
CGCATTGTTGCGCCCAGGTAAGAAAGTTTATTCTCACTCAGGTGATGGAGGTTTCCTCTTCACAGGTCAAGAACTCGAAA
CGGCCGTTCGTTTAAATCTTCCAATCGTTCAAATCATCTGGAATGATGGTCATTACGACATGGTAAAATTCCAAGAAGAA
ATGAAATATGGACGTTCAGCAGCCGTTGATTTCGGTTATGTTGATTACGTAAAATATGCTGAAGCAATGGGAGCAACAGG
CTACCGTGCCCACAGCAAAGAAGAACTTGCCGAAATTCTAAAATCAATTCCAAATACTACAGGTCCTGTAGTAATTGATG
TACCTTTGGACTACTCTGATAATATTAAGTTAGCAGAAAAATTATTGCCAGAAGAATTTTACTGA

Upstream 100 bases:

>100_bases
CAAAATAATTGTAAAAGGGTCTATTATCTGATAAAATGATTGTGAAGTAATCCAAGAGATTATGAAATATGAATTAGAAC
AAATAGAGGTAAAATAAAAA

Downstream 100 bases:

>100_bases
TAAAAAACAAGCAAATTGTGAAATAACAAAAATAAAAGAAGAAGTCCTTGAACCTCTAAGCGTTCAGGGGCTTTTTTTGT
GAAATAAAACAGGTGAAATT

Product: acetolactate synthase

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase [H]

Number of amino acids: Translated: 554; Mature: 553

Protein sequence:

>554_residues
MSEKQFGANLVVDSLINHKVKYVFGIPGAKIDRVFDLLENEEGPQMVVTRHEQGAAFMAQAVGRLTGEPGVVVVTSGPGV
SNLATPLLTATSEGDAILAIGGQVKRSDRLKRAHQSMDNAGMMQSATKYSAEVLDPDTLSESIANAYRIAKSGHPGATFL
SIPQDVTDAEVSIKAIQPLSDPKMGNASIDDINYLAQAIKNAVLPVILVGAGASDVKVASSLRNLLTHVNIPVVETFQGA
GVISHDLEHTFYGRIGLFRNQPGDMLLKRSDLVIAVGYDPIEYEARNWNAEIDSRIIVIDNAIAEIDTYYQPERELIGDI
AATLDNLLPAVRGYKIPKGTKEYLDGLHEVAEQHEFDTENTEEGRMHPLDLVSTFQEIVKDDETVTVDVGSLYIWMARHF
KSYEPRHLLFSNGMQTLGVALPWAITAALLRPGKKVYSHSGDGGFLFTGQELETAVRLNLPIVQIIWNDGHYDMVKFQEE
MKYGRSAAVDFGYVDYVKYAEAMGATGYRAHSKEELAEILKSIPNTTGPVVIDVPLDYSDNIKLAEKLLPEEFY

Sequences:

>Translated_554_residues
MSEKQFGANLVVDSLINHKVKYVFGIPGAKIDRVFDLLENEEGPQMVVTRHEQGAAFMAQAVGRLTGEPGVVVVTSGPGV
SNLATPLLTATSEGDAILAIGGQVKRSDRLKRAHQSMDNAGMMQSATKYSAEVLDPDTLSESIANAYRIAKSGHPGATFL
SIPQDVTDAEVSIKAIQPLSDPKMGNASIDDINYLAQAIKNAVLPVILVGAGASDVKVASSLRNLLTHVNIPVVETFQGA
GVISHDLEHTFYGRIGLFRNQPGDMLLKRSDLVIAVGYDPIEYEARNWNAEIDSRIIVIDNAIAEIDTYYQPERELIGDI
AATLDNLLPAVRGYKIPKGTKEYLDGLHEVAEQHEFDTENTEEGRMHPLDLVSTFQEIVKDDETVTVDVGSLYIWMARHF
KSYEPRHLLFSNGMQTLGVALPWAITAALLRPGKKVYSHSGDGGFLFTGQELETAVRLNLPIVQIIWNDGHYDMVKFQEE
MKYGRSAAVDFGYVDYVKYAEAMGATGYRAHSKEELAEILKSIPNTTGPVVIDVPLDYSDNIKLAEKLLPEEFY
>Mature_553_residues
SEKQFGANLVVDSLINHKVKYVFGIPGAKIDRVFDLLENEEGPQMVVTRHEQGAAFMAQAVGRLTGEPGVVVVTSGPGVS
NLATPLLTATSEGDAILAIGGQVKRSDRLKRAHQSMDNAGMMQSATKYSAEVLDPDTLSESIANAYRIAKSGHPGATFLS
IPQDVTDAEVSIKAIQPLSDPKMGNASIDDINYLAQAIKNAVLPVILVGAGASDVKVASSLRNLLTHVNIPVVETFQGAG
VISHDLEHTFYGRIGLFRNQPGDMLLKRSDLVIAVGYDPIEYEARNWNAEIDSRIIVIDNAIAEIDTYYQPERELIGDIA
ATLDNLLPAVRGYKIPKGTKEYLDGLHEVAEQHEFDTENTEEGRMHPLDLVSTFQEIVKDDETVTVDVGSLYIWMARHFK
SYEPRHLLFSNGMQTLGVALPWAITAALLRPGKKVYSHSGDGGFLFTGQELETAVRLNLPIVQIIWNDGHYDMVKFQEEM
KYGRSAAVDFGYVDYVKYAEAMGATGYRAHSKEELAEILKSIPNTTGPVVIDVPLDYSDNIKLAEKLLPEEFY

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=574, Percent_Identity=27.0034843205575, Blast_Score=187, Evalue=3e-47,
Organism=Homo sapiens, GI21361361, Length=584, Percent_Identity=23.2876712328767, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI1790104, Length=554, Percent_Identity=29.9638989169675, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI87081685, Length=558, Percent_Identity=28.3154121863799, Blast_Score=204, Evalue=9e-54,
Organism=Escherichia coli, GI1786717, Length=558, Percent_Identity=24.3727598566308, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1787096, Length=543, Percent_Identity=25.9668508287293, Blast_Score=145, Evalue=8e-36,
Organism=Escherichia coli, GI1788716, Length=552, Percent_Identity=24.6376811594203, Blast_Score=132, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI17531299, Length=550, Percent_Identity=27.2727272727273, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17531301, Length=550, Percent_Identity=27.2727272727273, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17542570, Length=549, Percent_Identity=25.1366120218579, Blast_Score=129, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6323755, Length=560, Percent_Identity=28.75, Blast_Score=205, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6320816, Length=474, Percent_Identity=23.6286919831224, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI19922626, Length=559, Percent_Identity=24.6869409660107, Blast_Score=150, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012782
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 60780; Mature: 60648

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKQFGANLVVDSLINHKVKYVFGIPGAKIDRVFDLLENEEGPQMVVTRHEQGAAFMAQ
CCCCHHCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHH
AVGRLTGEPGVVVVTSGPGVSNLATPLLTATSEGDAILAIGGQVKRSDRLKRAHQSMDNA
HHHHHCCCCCEEEEECCCCCHHHHHHHEEECCCCCEEEEECCCCHHHHHHHHHHHHHCCC
GMMQSATKYSAEVLDPDTLSESIANAYRIAKSGHPGATFLSIPQDVTDAEVSIKAIQPLS
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEEECCCC
DPKMGNASIDDINYLAQAIKNAVLPVILVGAGASDVKVASSLRNLLTHVNIPVVETFQGA
CCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCEECCCCC
GVISHDLEHTFYGRIGLFRNQPGDMLLKRSDLVIAVGYDPIEYEARNWNAEIDSRIIVID
CCCHHHHHHHHHHCEEEECCCCCCCEEEECCEEEEECCCCCEECCCCCCCCCCCEEEEEE
NAIAEIDTYYQPERELIGDIAATLDNLLPAVRGYKIPKGTKEYLDGLHEVAEQHEFDTEN
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCC
TEEGRMHPLDLVSTFQEIVKDDETVTVDVGSLYIWMARHFKSYEPRHLLFSNGMQTLGVA
CCCCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHH
LPWAITAALLRPGKKVYSHSGDGGFLFTGQELETAVRLNLPIVQIIWNDGHYDMVKFQEE
HHHHHHHHHHCCCHHHHCCCCCCCEEEECHHHCEEEEECCCEEEEEECCCCCHHHHHHHH
MKYGRSAAVDFGYVDYVKYAEAMGATGYRAHSKEELAEILKSIPNTTGPVVIDVPLDYSD
HHCCCCCEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCC
NIKLAEKLLPEEFY
CCHHHHHCCCCCCC
>Mature Secondary Structure 
SEKQFGANLVVDSLINHKVKYVFGIPGAKIDRVFDLLENEEGPQMVVTRHEQGAAFMAQ
CCCHHCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHH
AVGRLTGEPGVVVVTSGPGVSNLATPLLTATSEGDAILAIGGQVKRSDRLKRAHQSMDNA
HHHHHCCCCCEEEEECCCCCHHHHHHHEEECCCCCEEEEECCCCHHHHHHHHHHHHHCCC
GMMQSATKYSAEVLDPDTLSESIANAYRIAKSGHPGATFLSIPQDVTDAEVSIKAIQPLS
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEEECCCC
DPKMGNASIDDINYLAQAIKNAVLPVILVGAGASDVKVASSLRNLLTHVNIPVVETFQGA
CCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCEECCCCC
GVISHDLEHTFYGRIGLFRNQPGDMLLKRSDLVIAVGYDPIEYEARNWNAEIDSRIIVID
CCCHHHHHHHHHHCEEEECCCCCCCEEEECCEEEEECCCCCEECCCCCCCCCCCEEEEEE
NAIAEIDTYYQPERELIGDIAATLDNLLPAVRGYKIPKGTKEYLDGLHEVAEQHEFDTEN
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCC
TEEGRMHPLDLVSTFQEIVKDDETVTVDVGSLYIWMARHFKSYEPRHLLFSNGMQTLGVA
CCCCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHH
LPWAITAALLRPGKKVYSHSGDGGFLFTGQELETAVRLNLPIVQIIWNDGHYDMVKFQEE
HHHHHHHHHHCCCHHHHCCCCCCCEEEECHHHCEEEEECCCEEEEEECCCCCHHHHHHHH
MKYGRSAAVDFGYVDYVKYAEAMGATGYRAHSKEELAEILKSIPNTTGPVVIDVPLDYSD
HHCCCCCEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCC
NIKLAEKLLPEEFY
CCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7685336; 9353933; 9384377; 10809684 [H]