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The map label for this gene is vacB2 [H]

Identifier: 125624126

GI number: 125624126

Start: 1272922

End: 1274925

Strand: Reverse

Name: vacB2 [H]

Synonym: llmg_1304

Alternate gene names: 125624126

Gene position: 1274925-1272922 (Counterclockwise)

Preceding gene: 125624127

Following gene: 125624125

Centisome position: 50.4

GC content: 36.08

Gene sequence:

>2004_bases
ATGGTTCAGCTATCAGAATTAGCATCAGCACTTAAACAAACAGAAAGTAAAGGAGTATTTTCTAAACATCCTAAAGGCTT
TGGTTTTGTACATCCAGAGGGTGAGACAGACAAAACTAATGATATCTACATTGGTCAAAACGAAACTAAATTTGCAATGG
ATGGAGATAAGGTTACTGTTAAGGTTTTATACCCTAAGACAGAAAAACGGGGGGCAAGCGGACAAATTATTAAAATCAAT
GAACGAGCAGTCGTTGATACAGTTGGAACTTACCGTTCTTTATCTAATCGCCAAGCCAAAGCTTTGGGATATAAAGGTCG
AATCGAACTCTTTAATGACCGTATTTCAGATACTCTTTACATAAAACAGCCCTTAACAGGAGTACAAGAAGAAGATGTAG
TAAGTATTAAAGTTACTCAATATCCAACAGATACTAAAGCATTTGAAGGAAAAATTACGCAAATCATTGGTCATAAAGGT
GAAGTTGGCCTTGATATCTTAGAAGTTTTGTATGCTATGAAAATTCCACAAGAATTCTCAGCTGAAACATTAGCTGAGGC
TGAGGCATTTTCTGAAGAATTAACCCAAGAAGACTTGCAAGGACGTGAAGACTATCGTAATGAAATCACTTATACCATTG
ATGGAGAGGATTCTAAAGATTTAGATGATGCGATTCATGTTAAGAAATTATCGAATGGACATTTTGAATTAGGGGTCCAC
ATTGCTGATGTCAGTCATTATGTAACCGAAGGTTCTTCTTTGAATGAAGAAGCTTATGCGCGAGCAACCTCGGTTTATGT
GACTGATAGAGTAGTTCCAATGTTACCTGTCAGATTGTCTAATAACCTGTGCTCACTTAATGAAGCTCAAGAACGGCTAA
CAATGTCTTGCCTAATGGAAATTGATGATAAGGGCAAGATTATCACTTATAAGATATCTCCATCAGTCATAAAAACAACT
TACCGCATGACTTATAGCAATGTGAACAAGATGATTCATCAGGGGCAAGAAGGACACCGCGAAGCATTAGAAAAATATTC
TAAAATTGCTGATTCGATAGAAGTAGCCGTTGAACTTCATGAAATTCTCGAAACAATGCGAAAAGACAGAGGGATGATTG
AATTTGATGAGAGTGAAGCAAAAATAATTTTAGATGATGAGGGTTACCCAATTGAAATTGTAAAACGTGACCGTGATACT
GCAGAACGCATGATTGAATCCTTCATGCTTATGGCAAATGAAACTGTTGCTCTTGATTTCCAAAAGAAAAAATTACCTTC
ATTGTATCGTGTCCATGAAACTCCTAAGGAAAAAGCATTTGCTAAATTGATGGAAGCTGCTGCAGAAGCTGGATTTTCTT
TGAGTTCTGACTCTCATCAAGCCATAAATTTCTTTGCTGATGAGATTAAAGATACAAGTTTTGAAAAAGCCTTAACTTAT
CAACTTCGTCATACTATGTCAACAGCGGTTTATTCTGAAAAAAATACAAAACATTTTGGACTTGCAGCGACTAACTACAC
ACATTTCACAAGTCCAATTAGACGTTATCCTGACTTAATTATTCATCGTTTATTACATTTATATCCTTCTGACCATTCCA
ACCGTACTAAGGATGAATGGAAAGAACGTTTGCCTGAAATTGCTAGTCACTCTTCCGATATGGAACACCGTGCTGTTGTC
ACTGAACGGATTATTGATGCGATGAAGAAAGCAGAATACATGTTAGAACGTATCGGTGAGGTTTATACTGGTACGATCAC
AGGAGTACAAAAGTTTGGTATTTTTGTAGCACTAGATAATACTGTTGAAGGTTTAGTTCGAGTACCAAATTTACATACTG
GGACAACAGAAGAACTTGAATTTGACGAAGAAATGAGTATTATTAAAGGGAAAAAATCGGAAACAATTTATCAAGTTGGA
AAAGAAATAAAAATTCGTGTTATTGCTGCCAATAAACGAAAAGGAACAGTAGATTTTGAACAAATCGCTCCTGAAAAAAA
CTAG

Upstream 100 bases:

>100_bases
AAAGGCCTCTGAAAAGGTCTTTTTGTTTACTGATAGTCGAATATTACTTACTGATTATGTTATGATAGAATGAAACAACT
GGAGGAAGAAATAAACATAT

Downstream 100 bases:

>100_bases
CAATTTGCTAGTTTTTTTCTTATGTTTTTTATTAGCTAATATCGTCTTTACAGTATATTTTACATAACATAAATTATGTT
AGCTGTGTTATTTTGAGTAT

Product: putative exoribonuclease R

Products: NA

Alternate protein names: RNase R 2; VacB protein homolog 2 [H]

Number of amino acids: Translated: 667; Mature: 667

Protein sequence:

>667_residues
MVQLSELASALKQTESKGVFSKHPKGFGFVHPEGETDKTNDIYIGQNETKFAMDGDKVTVKVLYPKTEKRGASGQIIKIN
ERAVVDTVGTYRSLSNRQAKALGYKGRIELFNDRISDTLYIKQPLTGVQEEDVVSIKVTQYPTDTKAFEGKITQIIGHKG
EVGLDILEVLYAMKIPQEFSAETLAEAEAFSEELTQEDLQGREDYRNEITYTIDGEDSKDLDDAIHVKKLSNGHFELGVH
IADVSHYVTEGSSLNEEAYARATSVYVTDRVVPMLPVRLSNNLCSLNEAQERLTMSCLMEIDDKGKIITYKISPSVIKTT
YRMTYSNVNKMIHQGQEGHREALEKYSKIADSIEVAVELHEILETMRKDRGMIEFDESEAKIILDDEGYPIEIVKRDRDT
AERMIESFMLMANETVALDFQKKKLPSLYRVHETPKEKAFAKLMEAAAEAGFSLSSDSHQAINFFADEIKDTSFEKALTY
QLRHTMSTAVYSEKNTKHFGLAATNYTHFTSPIRRYPDLIIHRLLHLYPSDHSNRTKDEWKERLPEIASHSSDMEHRAVV
TERIIDAMKKAEYMLERIGEVYTGTITGVQKFGIFVALDNTVEGLVRVPNLHTGTTEELEFDEEMSIIKGKKSETIYQVG
KEIKIRVIAANKRKGTVDFEQIAPEKN

Sequences:

>Translated_667_residues
MVQLSELASALKQTESKGVFSKHPKGFGFVHPEGETDKTNDIYIGQNETKFAMDGDKVTVKVLYPKTEKRGASGQIIKIN
ERAVVDTVGTYRSLSNRQAKALGYKGRIELFNDRISDTLYIKQPLTGVQEEDVVSIKVTQYPTDTKAFEGKITQIIGHKG
EVGLDILEVLYAMKIPQEFSAETLAEAEAFSEELTQEDLQGREDYRNEITYTIDGEDSKDLDDAIHVKKLSNGHFELGVH
IADVSHYVTEGSSLNEEAYARATSVYVTDRVVPMLPVRLSNNLCSLNEAQERLTMSCLMEIDDKGKIITYKISPSVIKTT
YRMTYSNVNKMIHQGQEGHREALEKYSKIADSIEVAVELHEILETMRKDRGMIEFDESEAKIILDDEGYPIEIVKRDRDT
AERMIESFMLMANETVALDFQKKKLPSLYRVHETPKEKAFAKLMEAAAEAGFSLSSDSHQAINFFADEIKDTSFEKALTY
QLRHTMSTAVYSEKNTKHFGLAATNYTHFTSPIRRYPDLIIHRLLHLYPSDHSNRTKDEWKERLPEIASHSSDMEHRAVV
TERIIDAMKKAEYMLERIGEVYTGTITGVQKFGIFVALDNTVEGLVRVPNLHTGTTEELEFDEEMSIIKGKKSETIYQVG
KEIKIRVIAANKRKGTVDFEQIAPEKN
>Mature_667_residues
MVQLSELASALKQTESKGVFSKHPKGFGFVHPEGETDKTNDIYIGQNETKFAMDGDKVTVKVLYPKTEKRGASGQIIKIN
ERAVVDTVGTYRSLSNRQAKALGYKGRIELFNDRISDTLYIKQPLTGVQEEDVVSIKVTQYPTDTKAFEGKITQIIGHKG
EVGLDILEVLYAMKIPQEFSAETLAEAEAFSEELTQEDLQGREDYRNEITYTIDGEDSKDLDDAIHVKKLSNGHFELGVH
IADVSHYVTEGSSLNEEAYARATSVYVTDRVVPMLPVRLSNNLCSLNEAQERLTMSCLMEIDDKGKIITYKISPSVIKTT
YRMTYSNVNKMIHQGQEGHREALEKYSKIADSIEVAVELHEILETMRKDRGMIEFDESEAKIILDDEGYPIEIVKRDRDT
AERMIESFMLMANETVALDFQKKKLPSLYRVHETPKEKAFAKLMEAAAEAGFSLSSDSHQAINFFADEIKDTSFEKALTY
QLRHTMSTAVYSEKNTKHFGLAATNYTHFTSPIRRYPDLIIHRLLHLYPSDHSNRTKDEWKERLPEIASHSSDMEHRAVV
TERIIDAMKKAEYMLERIGEVYTGTITGVQKFGIFVALDNTVEGLVRVPNLHTGTTEELEFDEEMSIIKGKKSETIYQVG
KEIKIRVIAANKRKGTVDFEQIAPEKN

Specific function: 3'-5'exoribonuclease that participates in an essential cell function. Acts nonspecifically on poly(A), poly(U) and ribosomal RNAs [H]

COG id: COG0557

COG function: function code K; Exoribonuclease R

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI190014625, Length=470, Percent_Identity=29.1489361702128, Blast_Score=206, Evalue=6e-53,
Organism=Homo sapiens, GI190014623, Length=470, Percent_Identity=29.1489361702128, Blast_Score=206, Evalue=7e-53,
Organism=Homo sapiens, GI134288890, Length=415, Percent_Identity=31.3253012048193, Blast_Score=201, Evalue=2e-51,
Organism=Homo sapiens, GI219521928, Length=560, Percent_Identity=26.6071428571429, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI19115966, Length=560, Percent_Identity=26.6071428571429, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI156105693, Length=184, Percent_Identity=30.4347826086957, Blast_Score=88, Evalue=3e-17,
Organism=Homo sapiens, GI156105695, Length=184, Percent_Identity=30.4347826086957, Blast_Score=88, Evalue=3e-17,
Organism=Escherichia coli, GI87082383, Length=663, Percent_Identity=32.1266968325792, Blast_Score=330, Evalue=2e-91,
Organism=Escherichia coli, GI1787542, Length=664, Percent_Identity=24.2469879518072, Blast_Score=151, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI212645896, Length=555, Percent_Identity=28.8288288288288, Blast_Score=200, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17553506, Length=454, Percent_Identity=27.9735682819383, Blast_Score=180, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6324552, Length=524, Percent_Identity=29.0076335877863, Blast_Score=177, Evalue=7e-45,
Organism=Saccharomyces cerevisiae, GI6320499, Length=342, Percent_Identity=25.4385964912281, Blast_Score=87, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6323943, Length=355, Percent_Identity=23.943661971831, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24649634, Length=408, Percent_Identity=32.3529411764706, Blast_Score=209, Evalue=4e-54,
Organism=Drosophila melanogaster, GI24654592, Length=358, Percent_Identity=31.8435754189944, Blast_Score=174, Evalue=2e-43,
Organism=Drosophila melanogaster, GI19922976, Length=358, Percent_Identity=31.8435754189944, Blast_Score=174, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24654597, Length=358, Percent_Identity=31.8435754189944, Blast_Score=174, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013223
- InterPro:   IPR001900
- InterPro:   IPR022966
- InterPro:   IPR004476
- InterPro:   IPR011805 [H]

Pfam domain/function: PF08206 OB_RNB; PF00773 RNB; PF00575 S1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 75595; Mature: 75595

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS50126 S1 ; PS01175 RIBONUCLEASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQLSELASALKQTESKGVFSKHPKGFGFVHPEGETDKTNDIYIGQNETKFAMDGDKVTV
CCCHHHHHHHHHHHHCCCCHHCCCCCCEEECCCCCCCCCCCEEECCCCCEEEECCCEEEE
KVLYPKTEKRGASGQIIKINERAVVDTVGTYRSLSNRQAKALGYKGRIELFNDRISDTLY
EEEECCCCCCCCCCCEEEECCCEEEHHHHHHHHHCCCHHHHCCCCCCEEEECCCCCCEEE
IKQPLTGVQEEDVVSIKVTQYPTDTKAFEGKITQIIGHKGEVGLDILEVLYAMKIPQEFS
EECCCCCCCCCCEEEEEEEECCCCCHHHCCHHHHHHCCCCCCCHHHHHHHHHHCCCHHHH
AETLAEAEAFSEELTQEDLQGREDYRNEITYTIDGEDSKDLDDAIHVKKLSNGHFELGVH
HHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEE
IADVSHYVTEGSSLNEEAYARATSVYVTDRVVPMLPVRLSNNLCSLNEAQERLTMSCLME
EEHHHHHHHCCCCCCHHHHHHHHEEEEECCCCCEECHHHCCCCCCHHHHHHHHHHHHHHH
IDDKGKIITYKISPSVIKTTYRMTYSNVNKMIHQGQEGHREALEKYSKIADSIEVAVELH
CCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
EILETMRKDRGMIEFDESEAKIILDDEGYPIEIVKRDRDTAERMIESFMLMANETVALDF
HHHHHHHHCCCCEEECCCCCEEEECCCCCEEEEEECCHHHHHHHHHHHHHHCCCEEEEEE
QKKKLPSLYRVHETPKEKAFAKLMEAAAEAGFSLSSDSHQAINFFADEIKDTSFEKALTY
HHHHCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHH
QLRHTMSTAVYSEKNTKHFGLAATNYTHFTSPIRRYPDLIIHRLLHLYPSDHSNRTKDEW
HHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
KERLPEIASHSSDMEHRAVVTERIIDAMKKAEYMLERIGEVYTGTITGVQKFGIFVALDN
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCEEEEEECC
TVEGLVRVPNLHTGTTEELEFDEEMSIIKGKKSETIYQVGKEIKIRVIAANKRKGTVDFE
HHHHHEECCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHCCCEEEEEEEEECCCCCCCCHH
QIAPEKN
HCCCCCC
>Mature Secondary Structure
MVQLSELASALKQTESKGVFSKHPKGFGFVHPEGETDKTNDIYIGQNETKFAMDGDKVTV
CCCHHHHHHHHHHHHCCCCHHCCCCCCEEECCCCCCCCCCCEEECCCCCEEEECCCEEEE
KVLYPKTEKRGASGQIIKINERAVVDTVGTYRSLSNRQAKALGYKGRIELFNDRISDTLY
EEEECCCCCCCCCCCEEEECCCEEEHHHHHHHHHCCCHHHHCCCCCCEEEECCCCCCEEE
IKQPLTGVQEEDVVSIKVTQYPTDTKAFEGKITQIIGHKGEVGLDILEVLYAMKIPQEFS
EECCCCCCCCCCEEEEEEEECCCCCHHHCCHHHHHHCCCCCCCHHHHHHHHHHCCCHHHH
AETLAEAEAFSEELTQEDLQGREDYRNEITYTIDGEDSKDLDDAIHVKKLSNGHFELGVH
HHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEE
IADVSHYVTEGSSLNEEAYARATSVYVTDRVVPMLPVRLSNNLCSLNEAQERLTMSCLME
EEHHHHHHHCCCCCCHHHHHHHHEEEEECCCCCEECHHHCCCCCCHHHHHHHHHHHHHHH
IDDKGKIITYKISPSVIKTTYRMTYSNVNKMIHQGQEGHREALEKYSKIADSIEVAVELH
CCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
EILETMRKDRGMIEFDESEAKIILDDEGYPIEIVKRDRDTAERMIESFMLMANETVALDF
HHHHHHHHCCCCEEECCCCCEEEECCCCCEEEEEECCHHHHHHHHHHHHHHCCCEEEEEE
QKKKLPSLYRVHETPKEKAFAKLMEAAAEAGFSLSSDSHQAINFFADEIKDTSFEKALTY
HHHHCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHH
QLRHTMSTAVYSEKNTKHFGLAATNYTHFTSPIRRYPDLIIHRLLHLYPSDHSNRTKDEW
HHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
KERLPEIASHSSDMEHRAVVTERIIDAMKKAEYMLERIGEVYTGTITGVQKFGIFVALDN
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCEEEEEECC
TVEGLVRVPNLHTGTTEELEFDEEMSIIKGKKSETIYQVGKEIKIRVIAANKRKGTVDFE
HHHHHEECCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHCCCEEEEEEEEECCCCCCCCHH
QIAPEKN
HCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471; 1400209 [H]