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The map label for this gene is rlmA [H]

Identifier: 125623955

GI number: 125623955

Start: 1085751

End: 1086566

Strand: Reverse

Name: rlmA [H]

Synonym: llmg_1124

Alternate gene names: 125623955

Gene position: 1086566-1085751 (Counterclockwise)

Preceding gene: 125623956

Following gene: 125623954

Centisome position: 42.96

GC content: 34.8

Gene sequence:

>816_bases
ATGTCTGAAATTATAAAATGTCCTGTTTGCAATCATTTACTTACAAAAGAAGGAAATACTTTTAAATGTGAGAATAATCA
TACTTTTGATTTGGCTAAAGAAGGCTATCTTAACCTTCTTTTAAATGCCAAAAAAACTTCTGGCGACTCAAAAGAAATGA
TGGCAGCCAGACGTGATTTTCTCGCAAAAGGTTATTATGAAAAACTATCCGACCGAGTCAATCAACGCTTAAACAATTTG
TCTTCAACCAACCAAACCATTGTCGACATTGGTTGTGGGGAAGGTTACTATCTTTCACGTTTCCAAAAAGAAATGGCACC
AGAATCCACTAAGCTTTATGGAATGGATATTTCTAAGCTCGGGATTCGCATGGCTGCTAAAAAAAACACAACGATTCAAT
GGTTAGTAGCCAATTTTGCCAATCTCCCTTTTACTGACAAGTCTGTCAGTACTGTTTTATCCATGTTCGCAGAATATTCT
GTCCCAGAAATTGACCGAATTCTTACTGACGAAGGAAATATTATCATCGTCAGAGCCGCTAGCGACCACCTCATTGAATT
AAAAAATATTATCTACCCTGAAATTCATGAAAAAGTTAAAGCCTCTTCTATTAAAACTTTCCCTGGCTTTTTAGTTGAGC
AAGAAAATTATTCTTATCAAGTGACAATCCAATCCACAGAAGATTTATTAAGCTTACTACTGATGACCCCTCATTATTGG
AAAATCAAACCAGAGGGAATTGAAAATCTCAAAAAGTTTGAAGCTTTAGAAGTAACAATCAGTATTGAAATTGATGTATT
AAAGCGACAAAAATAA

Upstream 100 bases:

>100_bases
AAACGATTCGTGTCACTCCTGATGAAGCTGTCATTAAATCACGTAAAATCAGTAAGTAAAGTTTACTGACGAAAATTAAT
TTTAAAAATTTGGAGAATCC

Downstream 100 bases:

>100_bases
CTTTTTTTAATCGAAAAATAATCAACTTTTAAGTAATCATTTTCAAAATGCATTTTTAAGGAAAAGCCACGCGTTTATTG
CTATGACGGGGCTTTCATAT

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m1G745 methyltransferase; rRNA (guanine-N(1)-)-methyltransferase [H]

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MSEIIKCPVCNHLLTKEGNTFKCENNHTFDLAKEGYLNLLLNAKKTSGDSKEMMAARRDFLAKGYYEKLSDRVNQRLNNL
SSTNQTIVDIGCGEGYYLSRFQKEMAPESTKLYGMDISKLGIRMAAKKNTTIQWLVANFANLPFTDKSVSTVLSMFAEYS
VPEIDRILTDEGNIIIVRAASDHLIELKNIIYPEIHEKVKASSIKTFPGFLVEQENYSYQVTIQSTEDLLSLLLMTPHYW
KIKPEGIENLKKFEALEVTISIEIDVLKRQK

Sequences:

>Translated_271_residues
MSEIIKCPVCNHLLTKEGNTFKCENNHTFDLAKEGYLNLLLNAKKTSGDSKEMMAARRDFLAKGYYEKLSDRVNQRLNNL
SSTNQTIVDIGCGEGYYLSRFQKEMAPESTKLYGMDISKLGIRMAAKKNTTIQWLVANFANLPFTDKSVSTVLSMFAEYS
VPEIDRILTDEGNIIIVRAASDHLIELKNIIYPEIHEKVKASSIKTFPGFLVEQENYSYQVTIQSTEDLLSLLLMTPHYW
KIKPEGIENLKKFEALEVTISIEIDVLKRQK
>Mature_270_residues
SEIIKCPVCNHLLTKEGNTFKCENNHTFDLAKEGYLNLLLNAKKTSGDSKEMMAARRDFLAKGYYEKLSDRVNQRLNNLS
STNQTIVDIGCGEGYYLSRFQKEMAPESTKLYGMDISKLGIRMAAKKNTTIQWLVANFANLPFTDKSVSTVLSMFAEYSV
PEIDRILTDEGNIIIVRAASDHLIELKNIIYPEIHEKVKASSIKTFPGFLVEQENYSYQVTIQSTEDLLSLLLMTPHYWK
IKPEGIENLKKFEALEVTISIEIDVLKRQK

Specific function: Specifically methylates the guanosine in position 745 of 23S rRNA [H]

COG id: COG0500

COG function: function code QR; SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmA family [H]

Homologues:

Organism=Escherichia coli, GI1788125, Length=268, Percent_Identity=36.5671641791045, Blast_Score=182, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013216
- InterPro:   IPR016718 [H]

Pfam domain/function: PF08241 Methyltransf_11 [H]

EC number: =2.1.1.187 [H]

Molecular weight: Translated: 30892; Mature: 30761

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEIIKCPVCNHLLTKEGNTFKCENNHTFDLAKEGYLNLLLNAKKTSGDSKEMMAARRDF
CCCCEECCCHHHHHHCCCCEEEECCCCEEEHHHCCCEEEEEECCCCCCCHHHHHHHHHHH
LAKGYYEKLSDRVNQRLNNLSSTNQTIVDIGCGEGYYLSRFQKEMAPESTKLYGMDISKL
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCEEECCCHHHH
GIRMAAKKNTTIQWLVANFANLPFTDKSVSTVLSMFAEYSVPEIDRILTDEGNIIIVRAA
CEEEECCCCCEEEEEEHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHCCCCCCEEEEEEC
SDHLIELKNIIYPEIHEKVKASSIKTFPGFLVEQENYSYQVTIQSTEDLLSLLLMTPHYW
CCCHHHHHHHCCHHHHHHHHHCCCCCCCCEEEECCCCEEEEEEECHHHHHHHHHHCCCCE
KIKPEGIENLKKFEALEVTISIEIDVLKRQK
EECHHHHHHHHHHEEEEEEEEEEEEEECCCC
>Mature Secondary Structure 
SEIIKCPVCNHLLTKEGNTFKCENNHTFDLAKEGYLNLLLNAKKTSGDSKEMMAARRDF
CCCEECCCHHHHHHCCCCEEEECCCCEEEHHHCCCEEEEEECCCCCCCHHHHHHHHHHH
LAKGYYEKLSDRVNQRLNNLSSTNQTIVDIGCGEGYYLSRFQKEMAPESTKLYGMDISKL
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCEEECCCHHHH
GIRMAAKKNTTIQWLVANFANLPFTDKSVSTVLSMFAEYSVPEIDRILTDEGNIIIVRAA
CEEEECCCCCEEEEEEHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHCCCCCCEEEEEEC
SDHLIELKNIIYPEIHEKVKASSIKTFPGFLVEQENYSYQVTIQSTEDLLSLLLMTPHYW
CCCHHHHHHHCCHHHHHHHHHCCCCCCCCEEEECCCCEEEEEEECHHHHHHHHHHCCCCE
KIKPEGIENLKKFEALEVTISIEIDVLKRQK
EECHHHHHHHHHHEEEEEEEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7984109; 9097040; 9278503; 9440525 [H]