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The map label for this gene is ispB [H]

Identifier: 125623941

GI number: 125623941

Start: 1070361

End: 1071320

Strand: Reverse

Name: ispB [H]

Synonym: llmg_1110

Alternate gene names: 125623941

Gene position: 1071320-1070361 (Counterclockwise)

Preceding gene: 125623942

Following gene: 125623940

Centisome position: 42.35

GC content: 37.19

Gene sequence:

>960_bases
ATGGACTTAAAAAATAACTTTTGGAAAGACTATCCAGCACTTGAGAAGGAATTAGCTCAAGTACATCAATTAATGAAAAG
CCATGTTACCATCAAGAATTCTGATGTCAAATCGGCTATTTTTGATATTTTTGATGCAGGTGGGAAAATGTTACGCCCTG
CTTATCTGCTTCTATTTTCAGGATTCACTGACCTTTCGGAGAGCGAGCGATTGGCTCTTGCTGCTTCTGTCGAAATGATG
CACACGGCAACTCTAGTACATGATGATGTGATTGATCATGCTGATACCCGACGTGGTGTTGCCACCTTGTCAGCAAAATA
TGGGCCTGAAATTGCTGTTTATGCCGGCGACTATCTCTTTATTGCTGTCTTTCGTTTAATGTCAGAACACTCATTAGAAT
TGTCAAATTTAACTAAAAACATTGGGTCAATTGAGCGTTTGTTAGGTGGCGAATTGGGTCAACTTAATCATCATTTTAAT
CTTCAACAAACTTTGGATGATTATATTGAAAACATTTCGGGAAAAACGGGTGAACTTTTTGCACTATCTGCTTCTGTTGC
TCCTTTGATTTCCAAGAATAATGCTCTGACAAAACGAGCCTACAAAATCGGCATGAATATTGGAATTTCTTTCCAAATCA
TGGATGATTATTTGGACTATGCATCGACTGCTCAAACACTTGGTAAACCAGTCCTTGAGGACATCAAACAAGGCATCTAT
TCGGCTCCTGTCCTCTTTGCTTTACAGGAAAATAATGCTCTCGTTTCTGAATTAATTAAAAATGAAAAATTTGATGAAGT
TTATGATTTTATCAAAACTTCTGATGCCCTTGAAAAAACAAAAGCACTTGCGAAATCTTATACCCTCTCAGCTTTGAATT
TAATCGATAAATTACCTAAAGGTGAGAATCGAGAGCTGATTGCTGAAATTACTAGAAAACTTTTGGAGCGAACTTTATGA

Upstream 100 bases:

>100_bases
TTTTTGGAATACTAGCTGGTTTTGCGATTGGTATTGCCGGGAATCAATTAATTTCACGGGTTAAACCCATACAGGAACTA
TTTATCAAAGAGAGTAAACA

Downstream 100 bases:

>100_bases
CCCCTGATGAATTTTTGTCAGCACTGACAGACTTTGATATCAAGCTATCTGATAAACAAATCGAACAATTTGAACGTTAC
TTTGAACTTTTGGTTGAATG

Product: heptaprenyl diphosphate synthase component II

Products: NA

Alternate protein names: HEPPP synthase subunit 2; Spore germination protein C3 [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MDLKNNFWKDYPALEKELAQVHQLMKSHVTIKNSDVKSAIFDIFDAGGKMLRPAYLLLFSGFTDLSESERLALAASVEMM
HTATLVHDDVIDHADTRRGVATLSAKYGPEIAVYAGDYLFIAVFRLMSEHSLELSNLTKNIGSIERLLGGELGQLNHHFN
LQQTLDDYIENISGKTGELFALSASVAPLISKNNALTKRAYKIGMNIGISFQIMDDYLDYASTAQTLGKPVLEDIKQGIY
SAPVLFALQENNALVSELIKNEKFDEVYDFIKTSDALEKTKALAKSYTLSALNLIDKLPKGENRELIAEITRKLLERTL

Sequences:

>Translated_319_residues
MDLKNNFWKDYPALEKELAQVHQLMKSHVTIKNSDVKSAIFDIFDAGGKMLRPAYLLLFSGFTDLSESERLALAASVEMM
HTATLVHDDVIDHADTRRGVATLSAKYGPEIAVYAGDYLFIAVFRLMSEHSLELSNLTKNIGSIERLLGGELGQLNHHFN
LQQTLDDYIENISGKTGELFALSASVAPLISKNNALTKRAYKIGMNIGISFQIMDDYLDYASTAQTLGKPVLEDIKQGIY
SAPVLFALQENNALVSELIKNEKFDEVYDFIKTSDALEKTKALAKSYTLSALNLIDKLPKGENRELIAEITRKLLERTL
>Mature_319_residues
MDLKNNFWKDYPALEKELAQVHQLMKSHVTIKNSDVKSAIFDIFDAGGKMLRPAYLLLFSGFTDLSESERLALAASVEMM
HTATLVHDDVIDHADTRRGVATLSAKYGPEIAVYAGDYLFIAVFRLMSEHSLELSNLTKNIGSIERLLGGELGQLNHHFN
LQQTLDDYIENISGKTGELFALSASVAPLISKNNALTKRAYKIGMNIGISFQIMDDYLDYASTAQTLGKPVLEDIKQGIY
SAPVLFALQENNALVSELIKNEKFDEVYDFIKTSDALEKTKALAKSYTLSALNLIDKLPKGENRELIAEITRKLLERTL

Specific function: Supplies heptaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinone menaquinone-7 (MQ-7) [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=318, Percent_Identity=32.0754716981132, Blast_Score=146, Evalue=3e-35,
Organism=Escherichia coli, GI1789578, Length=298, Percent_Identity=27.1812080536913, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1786623, Length=237, Percent_Identity=29.957805907173, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17505681, Length=292, Percent_Identity=34.2465753424658, Blast_Score=148, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6319475, Length=378, Percent_Identity=30.1587301587302, Blast_Score=136, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6325188, Length=209, Percent_Identity=25.8373205741627, Blast_Score=63, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24651612, Length=311, Percent_Identity=29.5819935691318, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI281365769, Length=262, Percent_Identity=28.6259541984733, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24660002, Length=262, Percent_Identity=28.6259541984733, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014119
- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.30 [H]

Molecular weight: Translated: 35570; Mature: 35570

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLKNNFWKDYPALEKELAQVHQLMKSHVTIKNSDVKSAIFDIFDAGGKMLRPAYLLLFS
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
GFTDLSESERLALAASVEMMHTATLVHDDVIDHADTRRGVATLSAKYGPEIAVYAGDYLF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCEEEEEECHHHH
IAVFRLMSEHSLELSNLTKNIGSIERLLGGELGQLNHHFNLQQTLDDYIENISGKTGELF
HHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHCCCCCCCEE
ALSASVAPLISKNNALTKRAYKIGMNIGISFQIMDDYLDYASTAQTLGKPVLEDIKQGIY
EEECHHHHHHCCCCHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCHHHHHHHHHHHH
SAPVLFALQENNALVSELIKNEKFDEVYDFIKTSDALEKTKALAKSYTLSALNLIDKLPK
CCCEEEEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GENRELIAEITRKLLERTL
CCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDLKNNFWKDYPALEKELAQVHQLMKSHVTIKNSDVKSAIFDIFDAGGKMLRPAYLLLFS
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
GFTDLSESERLALAASVEMMHTATLVHDDVIDHADTRRGVATLSAKYGPEIAVYAGDYLF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCEEEEEECHHHH
IAVFRLMSEHSLELSNLTKNIGSIERLLGGELGQLNHHFNLQQTLDDYIENISGKTGELF
HHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHCCCCCCCEE
ALSASVAPLISKNNALTKRAYKIGMNIGISFQIMDDYLDYASTAQTLGKPVLEDIKQGIY
EEECHHHHHHCCCCHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCHHHHHHHHHHHH
SAPVLFALQENNALVSELIKNEKFDEVYDFIKTSDALEKTKALAKSYTLSALNLIDKLPK
CCCEEEEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GENRELIAEITRKLLERTL
CCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 2121900; 9720033 [H]