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The map label for this gene is gidB [H]

Identifier: 125623940

GI number: 125623940

Start: 1069648

End: 1070364

Strand: Reverse

Name: gidB [H]

Synonym: llmg_1109

Alternate gene names: 125623940

Gene position: 1070364-1069648 (Counterclockwise)

Preceding gene: 125623941

Following gene: 125623934

Centisome position: 42.32

GC content: 36.12

Gene sequence:

>717_bases
ATGACCCCTGATGAATTTTTGTCAGCACTGACAGACTTTGATATCAAGCTATCTGATAAACAAATCGAACAATTTGAACG
TTACTTTGAACTTTTGGTTGAATGGAATGAAAAAATTAATCTGACCGCAATTACCGAAAAAAACGAAGTCTATTTAAAAC
ATTTTTATGACTCGGTTGCGCCAATTTTGTATGGATTAATTACTGACCAGCCCGTCAGCATTTTAGATATTGGGGCTGGA
GCTGGTTTTCCTAGCTTACCAATGAAGATTATTTTTCCTGAACTAGAGGTAACAATCATTGACTCTTTAAACAAACGAAT
TAATTTTTTGAGTTTACTGACAGAGGAATTAGGCCTTGAAAATGTTACCTTGCTTCATGGACGAGCCGAAGATTTTGGAC
AAGATTCTAATTATCGCGCAACGTTTGATTTTGTTACTGCCCGTGCAGTTGCTAGACTCTCCGTCCTATCAGAATTTACA
ATTCCTTTTCTCAAGAAAAATGGTAATTTATTGTCATTAAAAGCAGCTCAATTTGAAGAAGAATTAACAGATGCTAAAAA
AGCCATTGCAATTCTGGGCGGAAAATTTATCAAGGAAATCGCTTATGAATTGCCTAATGGCGATGAACGCCACATTGCCG
TCATTGAAAAGAAAAAAGAAACACCAAAGAAATACCCACGTAAAGCAGGCACTCCTGCTAAAAGTCCAATAAAATAA

Upstream 100 bases:

>100_bases
CTTATACCCTCTCAGCTTTGAATTTAATCGATAAATTACCTAAAGGTGAGAATCGAGAGCTGATTGCTGAAATTACTAGA
AAACTTTTGGAGCGAACTTT

Downstream 100 bases:

>100_bases
AAAATCGGCTAATCATTGCCGATTTTTTTGGTCAGATTGCTAATAAGCACTGAGATTGAAACAATGGCCGTAAAGCTATT
TATAATCATTGCTGGTGCAG

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase [H]

Number of amino acids: Translated: 238; Mature: 237

Protein sequence:

>238_residues
MTPDEFLSALTDFDIKLSDKQIEQFERYFELLVEWNEKINLTAITEKNEVYLKHFYDSVAPILYGLITDQPVSILDIGAG
AGFPSLPMKIIFPELEVTIIDSLNKRINFLSLLTEELGLENVTLLHGRAEDFGQDSNYRATFDFVTARAVARLSVLSEFT
IPFLKKNGNLLSLKAAQFEEELTDAKKAIAILGGKFIKEIAYELPNGDERHIAVIEKKKETPKKYPRKAGTPAKSPIK

Sequences:

>Translated_238_residues
MTPDEFLSALTDFDIKLSDKQIEQFERYFELLVEWNEKINLTAITEKNEVYLKHFYDSVAPILYGLITDQPVSILDIGAG
AGFPSLPMKIIFPELEVTIIDSLNKRINFLSLLTEELGLENVTLLHGRAEDFGQDSNYRATFDFVTARAVARLSVLSEFT
IPFLKKNGNLLSLKAAQFEEELTDAKKAIAILGGKFIKEIAYELPNGDERHIAVIEKKKETPKKYPRKAGTPAKSPIK
>Mature_237_residues
TPDEFLSALTDFDIKLSDKQIEQFERYFELLVEWNEKINLTAITEKNEVYLKHFYDSVAPILYGLITDQPVSILDIGAGA
GFPSLPMKIIFPELEVTIIDSLNKRINFLSLLTEELGLENVTLLHGRAEDFGQDSNYRATFDFVTARAVARLSVLSEFTI
PFLKKNGNLLSLKAAQFEEELTDAKKAIAILGGKFIKEIAYELPNGDERHIAVIEKKKETPKKYPRKAGTPAKSPIK

Specific function: Specifically methylates the N7 position of a guanosine in 16S rRNA [H]

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family [H]

Homologues:

Organism=Escherichia coli, GI1790179, Length=215, Percent_Identity=33.953488372093, Blast_Score=111, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003682 [H]

Pfam domain/function: PF02527 GidB [H]

EC number: 2.1.-.-

Molecular weight: Translated: 26855; Mature: 26724

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPDEFLSALTDFDIKLSDKQIEQFERYFELLVEWNEKINLTAITEKNEVYLKHFYDSVA
CCHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHH
PILYGLITDQPVSILDIGAGAGFPSLPMKIIFPELEVTIIDSLNKRINFLSLLTEELGLE
HHHHHHHCCCCCEEEEECCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCC
NVTLLHGRAEDFGQDSNYRATFDFVTARAVARLSVLSEFTIPFLKKNGNLLSLKAAQFEE
CEEEEECCCHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEHHHHHHH
ELTDAKKAIAILGGKFIKEIAYELPNGDERHIAVIEKKKETPKKYPRKAGTPAKSPIK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TPDEFLSALTDFDIKLSDKQIEQFERYFELLVEWNEKINLTAITEKNEVYLKHFYDSVA
CHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHH
PILYGLITDQPVSILDIGAGAGFPSLPMKIIFPELEVTIIDSLNKRINFLSLLTEELGLE
HHHHHHHCCCCCEEEEECCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCC
NVTLLHGRAEDFGQDSNYRATFDFVTARAVARLSVLSEFTIPFLKKNGNLLSLKAAQFEE
CEEEEECCCHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEHHHHHHH
ELTDAKKAIAILGGKFIKEIAYELPNGDERHIAVIEKKKETPKKYPRKAGTPAKSPIK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]