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The map label for this gene is glpF2 [H]

Identifier: 125623928

GI number: 125623928

Start: 1056343

End: 1057071

Strand: Reverse

Name: glpF2 [H]

Synonym: llmg_1097

Alternate gene names: 125623928

Gene position: 1057071-1056343 (Counterclockwise)

Preceding gene: 125623929

Following gene: 125623918

Centisome position: 41.79

GC content: 42.94

Gene sequence:

>729_bases
ATGAACTCTACTTTTGTTCAATTCCTTGGAGAATTTATCGGGACATTTATCCTGATTCTACTTGGTAACGGGATTGTCTG
TGCTGTATCTTTGAAAAAAACGAAGGCAGAAGGTGCCGGTTGGATTGCCATTGCTTTTGGTTGGGGACTAGCTGTCATGT
TAGGTGTATATAGCTCTAGCTTTATGTCTGAAGCTCATTTGAATCCTGCTGTCACTATTGGTATGGCTTTAATTGGTAAT
ATTAGCTGGTCTCTTGTTCTTCCCTATATTGCTGGTCAATTCCTCGGAGCAATGCTTGCTAGCTTCATTCTTTGGCTCTT
CTACTATCCTCACTGGGTAGAAACAAAAGATGCTGCTGTAATTCTGGGCTGTCATTCGACAGGTCCAGCCATCCGCCATT
TCCCTTCCAATTTCTTTGGTGAAGTGATTGGAACCATGGTTTTACTGATTGGAATTCTTTCCTTTGGGCGAAATCACTTG
GCAGATGGTTTATCACCTGCCTTAGTTGGTGGATTAATTACCGCAATTGGCTTTTCTCTTGGAGCAACAACTGGTTATTC
TCTCAATCCAGCTCGTGACCTTGGTCCTCGCATCATGCACGCGCTCCTTCCAATTAAAAATAAGGGAACATCTGACTGGG
GCTACTCATGGGTTCCAGTCTTAGGACCTCTGGTAGGTGGTGCCTTAGGTTGTTTAATCTTTAACAGTATTAGCCCACTA
ATCAAATAA

Upstream 100 bases:

>100_bases
AGAAATGGGGAAAGATGAAGCATGGGAAAAAGAACAAGTTGAGAGCTTCCTTACTTTAGCTGATGGTTATATTTTAAAAT
AATTTTAGGAGAATATCTAT

Downstream 100 bases:

>100_bases
TATAAAAAACCTTCCATTTTATATCTGGAAGGTTTTTTGTTTTGGAAAAATTAGGGATTATTTTTTATTCAAATCATCAA
TTATTTTTTCTTGAGCTTCA

Product: glycerol uptake facilitator

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MNSTFVQFLGEFIGTFILILLGNGIVCAVSLKKTKAEGAGWIAIAFGWGLAVMLGVYSSSFMSEAHLNPAVTIGMALIGN
ISWSLVLPYIAGQFLGAMLASFILWLFYYPHWVETKDAAVILGCHSTGPAIRHFPSNFFGEVIGTMVLLIGILSFGRNHL
ADGLSPALVGGLITAIGFSLGATTGYSLNPARDLGPRIMHALLPIKNKGTSDWGYSWVPVLGPLVGGALGCLIFNSISPL
IK

Sequences:

>Translated_242_residues
MNSTFVQFLGEFIGTFILILLGNGIVCAVSLKKTKAEGAGWIAIAFGWGLAVMLGVYSSSFMSEAHLNPAVTIGMALIGN
ISWSLVLPYIAGQFLGAMLASFILWLFYYPHWVETKDAAVILGCHSTGPAIRHFPSNFFGEVIGTMVLLIGILSFGRNHL
ADGLSPALVGGLITAIGFSLGATTGYSLNPARDLGPRIMHALLPIKNKGTSDWGYSWVPVLGPLVGGALGCLIFNSISPL
IK
>Mature_242_residues
MNSTFVQFLGEFIGTFILILLGNGIVCAVSLKKTKAEGAGWIAIAFGWGLAVMLGVYSSSFMSEAHLNPAVTIGMALIGN
ISWSLVLPYIAGQFLGAMLASFILWLFYYPHWVETKDAAVILGCHSTGPAIRHFPSNFFGEVIGTMVLLIGILSFGRNHL
ADGLSPALVGGLITAIGFSLGATTGYSLNPARDLGPRIMHALLPIKNKGTSDWGYSWVPVLGPLVGGALGCLIFNSISPL
IK

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI157266307, Length=243, Percent_Identity=35.3909465020576, Blast_Score=122, Evalue=2e-28,
Organism=Homo sapiens, GI22538420, Length=245, Percent_Identity=34.2857142857143, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI4826645, Length=244, Percent_Identity=33.1967213114754, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI4502187, Length=247, Percent_Identity=30.3643724696356, Blast_Score=99, Evalue=2e-21,
Organism=Homo sapiens, GI37694062, Length=235, Percent_Identity=30.2127659574468, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1790362, Length=248, Percent_Identity=35.8870967741936, Blast_Score=107, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI71994009, Length=232, Percent_Identity=34.9137931034483, Blast_Score=117, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI71992966, Length=240, Percent_Identity=28.3333333333333, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17544068, Length=251, Percent_Identity=30.6772908366534, Blast_Score=104, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17533613, Length=233, Percent_Identity=30.4721030042918, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI32564052, Length=233, Percent_Identity=30.4721030042918, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17531429, Length=237, Percent_Identity=29.957805907173, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17531431, Length=237, Percent_Identity=29.957805907173, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71992961, Length=231, Percent_Identity=26.4069264069264, Blast_Score=87, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17558372, Length=229, Percent_Identity=27.0742358078603, Blast_Score=70, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6321054, Length=205, Percent_Identity=32.6829268292683, Blast_Score=104, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6322985, Length=286, Percent_Identity=26.2237762237762, Blast_Score=82, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 25616; Mature: 25616

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSTFVQFLGEFIGTFILILLGNGIVCAVSLKKTKAEGAGWIAIAFGWGLAVMLGVYSSS
CCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHCCCCCCEEEEHHHHHHHHHHHHHHHH
FMSEAHLNPAVTIGMALIGNISWSLVLPYIAGQFLGAMLASFILWLFYYPHWVETKDAAV
HHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
ILGCHSTGPAIRHFPSNFFGEVIGTMVLLIGILSFGRNHLADGLSPALVGGLITAIGFSL
EEEECCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCHHHHHHHHHHHHHHH
GATTGYSLNPARDLGPRIMHALLPIKNKGTSDWGYSWVPVLGPLVGGALGCLIFNSISPL
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IK
CC
>Mature Secondary Structure
MNSTFVQFLGEFIGTFILILLGNGIVCAVSLKKTKAEGAGWIAIAFGWGLAVMLGVYSSS
CCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHCCCCCCEEEEHHHHHHHHHHHHHHHH
FMSEAHLNPAVTIGMALIGNISWSLVLPYIAGQFLGAMLASFILWLFYYPHWVETKDAAV
HHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
ILGCHSTGPAIRHFPSNFFGEVIGTMVLLIGILSFGRNHLADGLSPALVGGLITAIGFSL
EEEECCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCHHHHHHHHHHHHHHH
GATTGYSLNPARDLGPRIMHALLPIKNKGTSDWGYSWVPVLGPLVGGALGCLIFNSISPL
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IK
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8436953; 9579061; 9384377; 2127799 [H]