Click here to switch to the map view.

The map label for this gene is trpE [H]

Identifier: 125623865

GI number: 125623865

Start: 994809

End: 996179

Strand: Direct

Name: trpE [H]

Synonym: llmg_1030

Alternate gene names: 125623865

Gene position: 994809-996179 (Clockwise)

Preceding gene: 125623864

Following gene: 125623866

Centisome position: 39.33

GC content: 37.13

Gene sequence:

>1371_bases
ATGAGAAAAATTAAAGAAATTTCAGCAGATACTTTGACGCCAATTTCGGTGTATTTTCGCCTGAAAGGTAAAAATAAAGT
AATTTTAGAATCCATTCCTAGAGAAAATGACCAATCACGTTTTTCAATTATTGCCTTAAATCCAGTAAAACATATTAAAT
TTACTGATGGAAATTTAACCATTAATGATGAAATCATTAGCAACGACCATCCAATGGAATTTCTAGAAAAATTGGTCTGT
CAGTCAGAAAATACGGACGAAAAATTGGATTTGCCCTTTACGAGCGGTGCAATTGGTTATGCTGGATTTGATACTTATGG
AATTTTTGAAGGGATTCAGCCTGAACTGACAGATACAATTGGGATCCCAGATATGTATTTTATGTTATACGAATCAGCCG
TGATTTTTGACCATAAACAAGAAAAATTGATATTTATTGAGGATAATGTTTATAGTCAGCGTTCAGAAAAAGAGTTAGTA
ACTGCTCTGTCAGCAAATATTGAAGCTTTATCGGAACTGACAGAAGCAGAAAATGAGTTGACAGAAATAAATAAAATGAA
TTTTGTCAGCAATATGACTCAAGCAACTTTTGAGGAAAAAGTAACAGCCGCAAAGGAATTGATTAGAAATGGTGATATGT
TTCAAATCGTTTTATCACAAAGGTTGACAGCCGATTTTACTGACAAGCCCTTTGATTATTATCGAAAACTTCGTGTTGAA
AATCCGTCATCATATATGTACTTTATGGAATTCGACAATTTTCATGTCATTGGCAGCTCACCAGAACGTTTGGTTGCGGT
TCATGGCAATCAAGTTTCGACTAATCCAATTGCTGGAACAAGAAAACGTGGCGACAATGAATTTGAAGACCAAGCGCTGA
TTGAAGAATTGGAAAATGACCAAAAAGAACTTGCTGAGCACAAAATGTTGGTTGATTTGGGACGAAATGACATTGGAAAA
ATTTCCGAATATGGCTCAATTGAAGTCCCTGTTTTCATGAAAGTTGAAAAATATCGCTACGTCATGCACATTACCAGTGA
AGTTACTGGAAAGCTTCGTCCCGAATTTACGGCCATGGACGCCCTGTCAGCGACTTTACCAGCTGGCACCCTATCAGGAG
CGCCGAAACATCGTGCTTATCAACGCATTTATGAGTTTGAAAATCAAAAACGCGGGATTTATGGCGGAGCCATTGGTTAT
CTGACCAAAAATGGAAATTGTGATTTTGCCATTGCCATTCGGACCATGGTCTTAAAAGATAATAAGGCACACGTTCAAGC
AGGTGCAGGAATTGTTTATGATTCAGTGCCTGAGCATGAATATCAAGAAACATTGAACAAGGCACAGGGACTGCTAAAAG
TTGGTCAGTAA

Upstream 100 bases:

>100_bases
TTTGAAAGGATTTCTAAAATGAATCAACGATGGCGAAGCTTAAATTTTATTTCATAATCAATGGAAGTTAATTCCGCAAA
ACAAAAGATAGAGAGAAAAC

Downstream 100 bases:

>100_bases
AAAAGGATGTGAAGTGTGCGAGCACACTGAACTCAACTAACAGGAGAAAAAATGATTTTAATAATTGATAATTACGACAG
TTTTACCTACAATTTGGTGC

Product: anthranilate synthase component I

Products: NA

Alternate protein names: Anthranilate synthase component I [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MRKIKEISADTLTPISVYFRLKGKNKVILESIPRENDQSRFSIIALNPVKHIKFTDGNLTINDEIISNDHPMEFLEKLVC
QSENTDEKLDLPFTSGAIGYAGFDTYGIFEGIQPELTDTIGIPDMYFMLYESAVIFDHKQEKLIFIEDNVYSQRSEKELV
TALSANIEALSELTEAENELTEINKMNFVSNMTQATFEEKVTAAKELIRNGDMFQIVLSQRLTADFTDKPFDYYRKLRVE
NPSSYMYFMEFDNFHVIGSSPERLVAVHGNQVSTNPIAGTRKRGDNEFEDQALIEELENDQKELAEHKMLVDLGRNDIGK
ISEYGSIEVPVFMKVEKYRYVMHITSEVTGKLRPEFTAMDALSATLPAGTLSGAPKHRAYQRIYEFENQKRGIYGGAIGY
LTKNGNCDFAIAIRTMVLKDNKAHVQAGAGIVYDSVPEHEYQETLNKAQGLLKVGQ

Sequences:

>Translated_456_residues
MRKIKEISADTLTPISVYFRLKGKNKVILESIPRENDQSRFSIIALNPVKHIKFTDGNLTINDEIISNDHPMEFLEKLVC
QSENTDEKLDLPFTSGAIGYAGFDTYGIFEGIQPELTDTIGIPDMYFMLYESAVIFDHKQEKLIFIEDNVYSQRSEKELV
TALSANIEALSELTEAENELTEINKMNFVSNMTQATFEEKVTAAKELIRNGDMFQIVLSQRLTADFTDKPFDYYRKLRVE
NPSSYMYFMEFDNFHVIGSSPERLVAVHGNQVSTNPIAGTRKRGDNEFEDQALIEELENDQKELAEHKMLVDLGRNDIGK
ISEYGSIEVPVFMKVEKYRYVMHITSEVTGKLRPEFTAMDALSATLPAGTLSGAPKHRAYQRIYEFENQKRGIYGGAIGY
LTKNGNCDFAIAIRTMVLKDNKAHVQAGAGIVYDSVPEHEYQETLNKAQGLLKVGQ
>Mature_456_residues
MRKIKEISADTLTPISVYFRLKGKNKVILESIPRENDQSRFSIIALNPVKHIKFTDGNLTINDEIISNDHPMEFLEKLVC
QSENTDEKLDLPFTSGAIGYAGFDTYGIFEGIQPELTDTIGIPDMYFMLYESAVIFDHKQEKLIFIEDNVYSQRSEKELV
TALSANIEALSELTEAENELTEINKMNFVSNMTQATFEEKVTAAKELIRNGDMFQIVLSQRLTADFTDKPFDYYRKLRVE
NPSSYMYFMEFDNFHVIGSSPERLVAVHGNQVSTNPIAGTRKRGDNEFEDQALIEELENDQKELAEHKMLVDLGRNDIGK
ISEYGSIEVPVFMKVEKYRYVMHITSEVTGKLRPEFTAMDALSATLPAGTLSGAPKHRAYQRIYEFENQKRGIYGGAIGY
LTKNGNCDFAIAIRTMVLKDNKAHVQAGAGIVYDSVPEHEYQETLNKAQGLLKVGQ

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=371, Percent_Identity=38.8140161725067, Blast_Score=240, Evalue=1e-64,
Organism=Escherichia coli, GI1788114, Length=421, Percent_Identity=33.2541567695962, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI1786809, Length=330, Percent_Identity=27.5757575757576, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI87082077, Length=260, Percent_Identity=28.4615384615385, Blast_Score=87, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320935, Length=454, Percent_Identity=38.5462555066079, Blast_Score=275, Evalue=8e-75,
Organism=Saccharomyces cerevisiae, GI6324361, Length=401, Percent_Identity=23.6907730673317, Blast_Score=115, Evalue=2e-26,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005256
- InterPro:   IPR015890 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 51680; Mature: 51680

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKIKEISADTLTPISVYFRLKGKNKVILESIPRENDQSRFSIIALNPVKHIKFTDGNLT
CCCHHHCCCCCCCEEEEEEEECCCCEEEEECCCCCCCCCEEEEEEECCCCEEEEECCCEE
INDEIISNDHPMEFLEKLVCQSENTDEKLDLPFTSGAIGYAGFDTYGIFEGIQPELTDTI
ECCCCCCCCCCHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCHHHHHCCCCCCHHHHC
GIPDMYFMLYESAVIFDHKQEKLIFIEDNVYSQRSEKELVTALSANIEALSELTEAENEL
CCCHHHHHHHHHHHEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TEINKMNFVSNMTQATFEEKVTAAKELIRNGDMFQIVLSQRLTADFTDKPFDYYRKLRVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHCCCCCCCCHHHHHHHCCC
NPSSYMYFMEFDNFHVIGSSPERLVAVHGNQVSTNPIAGTRKRGDNEFEDQALIEELEND
CCCCEEEEEEECCEEEECCCCCEEEEEECCEECCCCCCCCCCCCCCCHHHHHHHHHHHHH
QKELAEHKMLVDLGRNDIGKISEYGSIEVPVFMKVEKYRYVMHITSEVTGKLRPEFTAMD
HHHHHHHHHHHHCCCCCCCCHHCCCCEECEEEEEEEHEEEEEEEEHHHCCCCCCCHHHHH
ALSATLPAGTLSGAPKHRAYQRIYEFENQKRGIYGGAIGYLTKNGNCDFAIAIRTMVLKD
HHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCEEEEEEEEEEEC
NKAHVQAGAGIVYDSVPEHEYQETLNKAQGLLKVGQ
CCCEEEECCCEEECCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRKIKEISADTLTPISVYFRLKGKNKVILESIPRENDQSRFSIIALNPVKHIKFTDGNLT
CCCHHHCCCCCCCEEEEEEEECCCCEEEEECCCCCCCCCEEEEEEECCCCEEEEECCCEE
INDEIISNDHPMEFLEKLVCQSENTDEKLDLPFTSGAIGYAGFDTYGIFEGIQPELTDTI
ECCCCCCCCCCHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCHHHHHCCCCCCHHHHC
GIPDMYFMLYESAVIFDHKQEKLIFIEDNVYSQRSEKELVTALSANIEALSELTEAENEL
CCCHHHHHHHHHHHEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TEINKMNFVSNMTQATFEEKVTAAKELIRNGDMFQIVLSQRLTADFTDKPFDYYRKLRVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHCCCCCCCCHHHHHHHCCC
NPSSYMYFMEFDNFHVIGSSPERLVAVHGNQVSTNPIAGTRKRGDNEFEDQALIEELEND
CCCCEEEEEEECCEEEECCCCCEEEEEECCEECCCCCCCCCCCCCCCHHHHHHHHHHHHH
QKELAEHKMLVDLGRNDIGKISEYGSIEVPVFMKVEKYRYVMHITSEVTGKLRPEFTAMD
HHHHHHHHHHHHCCCCCCCCHHCCCCEECEEEEEEEHEEEEEEEEHHHCCCCCCCHHHHH
ALSATLPAGTLSGAPKHRAYQRIYEFENQKRGIYGGAIGYLTKNGNCDFAIAIRTMVLKD
HHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCEEEEEEEEEEEC
NKAHVQAGAGIVYDSVPEHEYQETLNKAQGLLKVGQ
CCCEEEECCCEEECCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1400208; 11337471 [H]