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The map label for this gene is hmcM [H]

Identifier: 125623770

GI number: 125623770

Start: 898471

End: 899625

Strand: Reverse

Name: hmcM [H]

Synonym: llmg_0929

Alternate gene names: 125623770

Gene position: 899625-898471 (Counterclockwise)

Preceding gene: 125623790

Following gene: 125623767

Centisome position: 35.57

GC content: 37.4

Gene sequence:

>1155_bases
ATGAAAGTCGGTATTGATAAACTTGGATTTTTTGTTCCCAGTACTTTTGTGGACATGAGGGATTTAGCAGAAGCACGCAA
TGTTGATCCAGCAAAATTCCAGATAGGTATTGGTCAAGATGAAATGGCAGTGAATCCTGCTACTCAAGATATAATTACTT
TTGCGGCTAATGCAGCAGCTAGTATTTTGACTGATGAAGATAAAGAAACGATTGACATGGTTATTGTTGGAACAGAATCA
AGTCTTGATGAATCAAAAGCTTCGGCGGTTGTTGTTCATGATTTACTCGGCATCCAACCTTTTGCTAGAAGTATCGAGAT
GAAGGAAGCTTGTTATGCAACAACTGCTGGCCTTGCTTTGGCCCGGGACCATGTTCTTTTGAACCCCGAAGCTAAAGTTT
TAGTCATCGCATCTGATATTGCTAAGTATGGTCTAAATACTGGTGGGGAACCTACTCAAGGGGCTGGAAGTGTTGCAATG
CTCATCACTGCTAATCCTAAAATTATGACCTTAAATAATGATAATGTTGCTTTAACTCAAGATATCTATGATTTCTGGCG
ACCTTTTGGTCAAGCTTACCCTACTGTTGATGGCAAATTTTCAAATGAAACTTACATTGATGCCTTTGCTAAAATTTGGA
AAGAATACAGTCGCCGAACAAGCTTGCACTTTGAAGATTTTGCTGCTCTTGCTTTTCATACCCCTTACACAAAAATGGGT
AAAAAAGCTTTGTTGCCAATGCTTGAAATAGAGAAACCAGCTAATGCTGAAGAACTGATGGAACAATTTGAACGTGGAAT
TGTTTATAATCGTCGTGTTGGTAATTTATATACTGGTTCTCTCTATTTAAGTCTAATCTCACTTTTAGAAAACTCTGATA
AATTGAATGCTGGTCAGCGTATTGGACTTTTTAGCTATGGCTCTGGTACGGTTGCCGAATTTTTCTCTGGTGAACTCGTC
GAAGGTTATGAAAAGCACTTGCTTAAAGTTGAACATCAATCAATGTTAGATGCAAGAAATCGCTTGAGCATTCCTGAATA
CGAAACTATGTTTAACGAATATTTAGACCTAAATCACAACATTTCATTTAGTGATGATACAGCCTATTCTGTTTCAGAAG
TCGTTGATAACCATCGTAAATATAAAAAAATATAA

Upstream 100 bases:

>100_bases
GAATTTAACGTATTTTTATGATAAAATTAAAGCATTCCAAAGACATTTGAAAGGAGTTCCTTTCTTCTATAATATTAGTA
GATAAGGAAATTTTTAAAAT

Downstream 100 bases:

>100_bases
AAAAATACTGACAGCTTTTCTGTCAGTAAATTAAAAAGTCCTGATAGGACTTTTTTTATTTTATTTGCTCAACAATGATA
CCGCAGCTTCGTCAGCAATA

Product: hydroxymethylglutaryl-CoA synthase

Products: (S)-3-hydroxy-3-methylglutaryl-CoA; CoA

Alternate protein names: HMG synthase [H]

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MKVGIDKLGFFVPSTFVDMRDLAEARNVDPAKFQIGIGQDEMAVNPATQDIITFAANAAASILTDEDKETIDMVIVGTES
SLDESKASAVVVHDLLGIQPFARSIEMKEACYATTAGLALARDHVLLNPEAKVLVIASDIAKYGLNTGGEPTQGAGSVAM
LITANPKIMTLNNDNVALTQDIYDFWRPFGQAYPTVDGKFSNETYIDAFAKIWKEYSRRTSLHFEDFAALAFHTPYTKMG
KKALLPMLEIEKPANAEELMEQFERGIVYNRRVGNLYTGSLYLSLISLLENSDKLNAGQRIGLFSYGSGTVAEFFSGELV
EGYEKHLLKVEHQSMLDARNRLSIPEYETMFNEYLDLNHNISFSDDTAYSVSEVVDNHRKYKKI

Sequences:

>Translated_384_residues
MKVGIDKLGFFVPSTFVDMRDLAEARNVDPAKFQIGIGQDEMAVNPATQDIITFAANAAASILTDEDKETIDMVIVGTES
SLDESKASAVVVHDLLGIQPFARSIEMKEACYATTAGLALARDHVLLNPEAKVLVIASDIAKYGLNTGGEPTQGAGSVAM
LITANPKIMTLNNDNVALTQDIYDFWRPFGQAYPTVDGKFSNETYIDAFAKIWKEYSRRTSLHFEDFAALAFHTPYTKMG
KKALLPMLEIEKPANAEELMEQFERGIVYNRRVGNLYTGSLYLSLISLLENSDKLNAGQRIGLFSYGSGTVAEFFSGELV
EGYEKHLLKVEHQSMLDARNRLSIPEYETMFNEYLDLNHNISFSDDTAYSVSEVVDNHRKYKKI
>Mature_384_residues
MKVGIDKLGFFVPSTFVDMRDLAEARNVDPAKFQIGIGQDEMAVNPATQDIITFAANAAASILTDEDKETIDMVIVGTES
SLDESKASAVVVHDLLGIQPFARSIEMKEACYATTAGLALARDHVLLNPEAKVLVIASDIAKYGLNTGGEPTQGAGSVAM
LITANPKIMTLNNDNVALTQDIYDFWRPFGQAYPTVDGKFSNETYIDAFAKIWKEYSRRTSLHFEDFAALAFHTPYTKMG
KKALLPMLEIEKPANAEELMEQFERGIVYNRRVGNLYTGSLYLSLISLLENSDKLNAGQRIGLFSYGSGTVAEFFSGELV
EGYEKHLLKVEHQSMLDARNRLSIPEYETMFNEYLDLNHNISFSDDTAYSVSEVVDNHRKYKKI

Specific function: Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. It catalyzes the aldol condensation between the acetyl group attached to the acyl-carrier-protein AcpK (Ac- AcpK) and

COG id: COG3425

COG function: function code I; 3-hydroxy-3-methylglutaryl CoA synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMG-CoA synthase family [H]

Homologues:

Organism=Homo sapiens, GI148298764, Length=375, Percent_Identity=29.0666666666667, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI148298677, Length=375, Percent_Identity=29.0666666666667, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI5031751, Length=449, Percent_Identity=26.2806236080178, Blast_Score=132, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17560126, Length=359, Percent_Identity=26.1838440111421, Blast_Score=138, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6323509, Length=397, Percent_Identity=27.9596977329975, Blast_Score=130, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24654139, Length=426, Percent_Identity=26.7605633802817, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24654137, Length=426, Percent_Identity=26.7605633802817, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24654133, Length=426, Percent_Identity=26.7605633802817, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24654135, Length=426, Percent_Identity=26.7605633802817, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI17933694, Length=426, Percent_Identity=26.7605633802817, Blast_Score=135, Evalue=6e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013746
- InterPro:   IPR013528
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF08540 HMG_CoA_synt_C; PF01154 HMG_CoA_synt_N [H]

EC number: 2.3.3.10

Molecular weight: Translated: 42667; Mature: 42667

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVGIDKLGFFVPSTFVDMRDLAEARNVDPAKFQIGIGQDEMAVNPATQDIITFAANAAA
CCCCHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHH
SILTDEDKETIDMVIVGTESSLDESKASAVVVHDLLGIQPFARSIEMKEACYATTAGLAL
HHHCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
ARDHVLLNPEAKVLVIASDIAKYGLNTGGEPTQGAGSVAMLITANPKIMTLNNDNVALTQ
HCCCEEECCCCCEEEEEHHHHHHCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCEEEHH
DIYDFWRPFGQAYPTVDGKFSNETYIDAFAKIWKEYSRRTSLHFEDFAALAFHTPYTKMG
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHH
KKALLPMLEIEKPANAEELMEQFERGIVYNRRVGNLYTGSLYLSLISLLENSDKLNAGQR
HHHHCCHHCCCCCCCHHHHHHHHHCCCEEECCCCCEEHHHHHHHHHHHHHCCCCCCCCCE
IGLFSYGSGTVAEFFSGELVEGYEKHLLKVEHQSMLDARNRLSIPEYETMFNEYLDLNHN
EEEEECCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCC
ISFSDDTAYSVSEVVDNHRKYKKI
CCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKVGIDKLGFFVPSTFVDMRDLAEARNVDPAKFQIGIGQDEMAVNPATQDIITFAANAAA
CCCCHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHH
SILTDEDKETIDMVIVGTESSLDESKASAVVVHDLLGIQPFARSIEMKEACYATTAGLAL
HHHCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
ARDHVLLNPEAKVLVIASDIAKYGLNTGGEPTQGAGSVAMLITANPKIMTLNNDNVALTQ
HCCCEEECCCCCEEEEEHHHHHHCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCEEEHH
DIYDFWRPFGQAYPTVDGKFSNETYIDAFAKIWKEYSRRTSLHFEDFAALAFHTPYTKMG
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHH
KKALLPMLEIEKPANAEELMEQFERGIVYNRRVGNLYTGSLYLSLISLLENSDKLNAGQR
HHHHCCHHCCCCCCCHHHHHHHHHCCCEEECCCCCEEHHHHHHHHHHHHHCCCCCCCCCE
IGLFSYGSGTVAEFFSGELVEGYEKHLLKVEHQSMLDARNRLSIPEYETMFNEYLDLNHN
EEEEECCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCC
ISFSDDTAYSVSEVVDNHRKYKKI
CCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acetyl-CoA; H2O; acetoacetyl-CoA

Specific reaction: acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704258; 9384377 [H]