Click here to switch to the map view.

The map label for this gene is queA

Identifier: 125623767

GI number: 125623767

Start: 895195

End: 896235

Strand: Reverse

Name: queA

Synonym: llmg_0926

Alternate gene names: 125623767

Gene position: 896235-895195 (Counterclockwise)

Preceding gene: 125623770

Following gene: 125623760

Centisome position: 35.43

GC content: 38.42

Gene sequence:

>1041_bases
ATGAATATAAATGATTTTGATTTTGATTTACCAGAAGAATTAATTGCACAAACGCCTTTGGAAAAACGCTCTGAATCACG
TTTATTGATTCTCGACCCCAAAACAGAAGAACTTGAAGATAGACATTTTTATGATATTATTGATGAACTCGAGGCCGGAG
ATGCTTTAGTTTTAAATAATACACGTGTGCTTCCTGCTCGTTTGCACGGTGAACGTGCTGAAACGGGTGGTCATATCGAA
CTACTTCTGCTCAAAGATATGGGAGAAAATCGTTGGGAAACTTTAGCTAAACCTGCTCGTAAAATGAAAGTTGGCGAGGA
AGTTGTTTTTGGTGACGGTCGTTTGAAAGCTGTTGTTGTTGAAATTCTCGAACACGGTGGTCGAATTGTTGAATTTAAAT
ATGACGGAATTTTTCTCGAAATCTTGGAAAGCTTAGGAGAAATGCCATTACCACCATATATTCATGAGCAACTTGAAGAC
CAAGAACGTTATCAAACCGTTTTTGCTAAAGAAAATGGTTCTGCCGCAGCTCCAACTGCTGGTTTGCATTATACACCTGA
ACTTTTAGAAAAAATTGCTGACAAAGGGGTTAAAATCGTTGAGTTGACTCTCCACGTTGGCTTAGGAACTTTTCGTCCTG
TCAGTGTAGATAATGTTGATGAACATCATATGCATAGTGAATTTTATCGTCTGACAGAGGAGGCTGCCGCCCAACTCCGA
GCCGTTAAAGCCAGTGGTCATAAGATTTTCGCTTCTGGAACAACGTCAATTCGTACTTTAGAAACAATTGGTAGCAAATT
TGATGGAGATATTCAAGCTGATTCTGGTTGGACTGATATTTTCATTAAACCTGGTTATGAATGGAAAGTTGTCGATGCCT
TTAATACTAATTTCCACTTACCAAAATCAACTTTAGTTATGTTAGTTGCCGCTTTTGCTGGACGTGATTTCGTTCTTGAG
GCTTATCAACACGCAATTGATGAAAAGTATCGCTTCTTCAGTTTTGGTGACGCCATGTTTGTTCGTTCAAAAAATAAATA
A

Upstream 100 bases:

>100_bases
ATAGACCTGAAAAGAAAGGCTTGAGGAAAATCTAAAATAGTTTAGCATAAAATATATTTTAGACCCTTGATAAAAGATTG
GAGTAAAATAAAAACAACTT

Downstream 100 bases:

>100_bases
ATTCTAATTAATTTTTATAAAAAAAGCCTCATAAGGCTTTTTTTATTTATAGTTTCTACTCACTTTACTTTACTTTATTT
AGAAGACAACAACCATTCTT

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MNINDFDFDLPEELIAQTPLEKRSESRLLILDPKTEELEDRHFYDIIDELEAGDALVLNNTRVLPARLHGERAETGGHIE
LLLLKDMGENRWETLAKPARKMKVGEEVVFGDGRLKAVVVEILEHGGRIVEFKYDGIFLEILESLGEMPLPPYIHEQLED
QERYQTVFAKENGSAAAPTAGLHYTPELLEKIADKGVKIVELTLHVGLGTFRPVSVDNVDEHHMHSEFYRLTEEAAAQLR
AVKASGHKIFASGTTSIRTLETIGSKFDGDIQADSGWTDIFIKPGYEWKVVDAFNTNFHLPKSTLVMLVAAFAGRDFVLE
AYQHAIDEKYRFFSFGDAMFVRSKNK

Sequences:

>Translated_346_residues
MNINDFDFDLPEELIAQTPLEKRSESRLLILDPKTEELEDRHFYDIIDELEAGDALVLNNTRVLPARLHGERAETGGHIE
LLLLKDMGENRWETLAKPARKMKVGEEVVFGDGRLKAVVVEILEHGGRIVEFKYDGIFLEILESLGEMPLPPYIHEQLED
QERYQTVFAKENGSAAAPTAGLHYTPELLEKIADKGVKIVELTLHVGLGTFRPVSVDNVDEHHMHSEFYRLTEEAAAQLR
AVKASGHKIFASGTTSIRTLETIGSKFDGDIQADSGWTDIFIKPGYEWKVVDAFNTNFHLPKSTLVMLVAAFAGRDFVLE
AYQHAIDEKYRFFSFGDAMFVRSKNK
>Mature_346_residues
MNINDFDFDLPEELIAQTPLEKRSESRLLILDPKTEELEDRHFYDIIDELEAGDALVLNNTRVLPARLHGERAETGGHIE
LLLLKDMGENRWETLAKPARKMKVGEEVVFGDGRLKAVVVEILEHGGRIVEFKYDGIFLEILESLGEMPLPPYIHEQLED
QERYQTVFAKENGSAAAPTAGLHYTPELLEKIADKGVKIVELTLHVGLGTFRPVSVDNVDEHHMHSEFYRLTEEAAAQLR
AVKASGHKIFASGTTSIRTLETIGSKFDGDIQADSGWTDIFIKPGYEWKVVDAFNTNFHLPKSTLVMLVAAFAGRDFVLE
AYQHAIDEKYRFFSFGDAMFVRSKNK

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=346, Percent_Identity=47.3988439306358, Blast_Score=305, Evalue=2e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_LACLM (A2RJR5)

Other databases:

- EMBL:   AM406671
- RefSeq:   YP_001032250.1
- ProteinModelPortal:   A2RJR5
- SMR:   A2RJR5
- STRING:   A2RJR5
- GeneID:   4798830
- GenomeReviews:   AM406671_GR
- KEGG:   llm:llmg_0926
- eggNOG:   COG0809
- HOGENOM:   HBG286076
- OMA:   SEFYRLT
- ProtClustDB:   PRK00147
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 39104; Mature: 39104

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNINDFDFDLPEELIAQTPLEKRSESRLLILDPKTEELEDRHFYDIIDELEAGDALVLNN
CCCCCCCCCCCHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEC
TRVLPARLHGERAETGGHIELLLLKDMGENRWETLAKPARKMKVGEEVVFGDGRLKAVVV
CEEEEHHHCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHH
EILEHGGRIVEFKYDGIFLEILESLGEMPLPPYIHEQLEDQERYQTVFAKENGSAAAPTA
HHHHCCCEEEEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCC
GLHYTPELLEKIADKGVKIVELTLHVGLGTFRPVSVDNVDEHHMHSEFYRLTEEAAAQLR
CCCCCHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AVKASGHKIFASGTTSIRTLETIGSKFDGDIQADSGWTDIFIKPGYEWKVVDAFNTNFHL
HHHCCCCEEEECCCCCHHHHHHHCCCCCCCEECCCCCEEEEEECCCCEEEEEECCCCCCC
PKSTLVMLVAAFAGRDFVLEAYQHAIDEKYRFFSFGDAMFVRSKNK
CHHHHHHHHHHHCCCHHHHHHHHHHHHHCEEEEEECCEEEEECCCC
>Mature Secondary Structure
MNINDFDFDLPEELIAQTPLEKRSESRLLILDPKTEELEDRHFYDIIDELEAGDALVLNN
CCCCCCCCCCCHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEC
TRVLPARLHGERAETGGHIELLLLKDMGENRWETLAKPARKMKVGEEVVFGDGRLKAVVV
CEEEEHHHCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHH
EILEHGGRIVEFKYDGIFLEILESLGEMPLPPYIHEQLEDQERYQTVFAKENGSAAAPTA
HHHHCCCEEEEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCC
GLHYTPELLEKIADKGVKIVELTLHVGLGTFRPVSVDNVDEHHMHSEFYRLTEEAAAQLR
CCCCCHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AVKASGHKIFASGTTSIRTLETIGSKFDGDIQADSGWTDIFIKPGYEWKVVDAFNTNFHL
HHHCCCCEEEECCCCCHHHHHHHCCCCCCCEECCCCCEEEEEECCCCEEEEEECCCCCCC
PKSTLVMLVAAFAGRDFVLEAYQHAIDEKYRFFSFGDAMFVRSKNK
CHHHHHHHHHHHCCCHHHHHHHHHHHHHCEEEEEECCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA