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The map label for this gene is dexC [H]

Identifier: 125623594

GI number: 125623594

Start: 722564

End: 724318

Strand: Direct

Name: dexC [H]

Synonym: llmg_0740

Alternate gene names: 125623594

Gene position: 722564-724318 (Clockwise)

Preceding gene: 125623590

Following gene: 125623595

Centisome position: 28.57

GC content: 36.41

Gene sequence:

>1755_bases
ATGAACAAAGCCGCAATTTATCATCGTCCTGAATCAGAGTTTGCTTATCTTTATGCAAAAAATCTGATGCATGTTCGTCT
TCAAACACAAAAGTCTGATGTGGAAAAAGTCGAATTATTTTATGGCGACCCTTATTCGTGGGAAGGCCAAGATAAGCCTG
ATACACAGTACTGGGCTAGTCAAAAAGTAGAGATGAAAAAGTATCTTTCTACTAATATTAGCGATTATTTTGAGGCCGAA
ATTACTGTTGCAACAAAACGTGTGGATTACTTATTCGTCATCACTGGTTTGGATGGAGAAAAAGTAGTTTATACTGACCA
AGGAATGTTTGATTATGATGAAGCCTTGGTAACTCAAAAATATGGAGCTTTTCGTTTGCCATATTTCCATGAAAGTGACC
GATTTAAAGCACCTGAATGGGTCAAAGAAACAGTTTGGTATCAAATTTTCCCTGAACGCTTTGCCAATGGCGATACAAGC
AATGACCCTGAAGGAACGAAAGCTTGGAATCCCCAAGATGTTCCCGACCGTCAAGATTTTTATGGGGGAGATTTGCAAGG
GGTGATTGATCATTTGGAACATCTGACTGAACTTGGTGTGAATGGTATTTACTTTACCCCTATTTTCCAAGCTTTTTCTA
ATCATAAATATGATACAGAAGATTATATGGAAATTGATAAGCAATTTGGGGATAAGGAATTGTTTAAAACTTTGGTGAAA
GAAGCACATGCGCGTGGTATTAAAGTCATGTTAGATGCAGTCTTTAACCATATTGGTGACACTTCTGCACAATGGCAAGA
TGTTTTGAAAAATCAAGAAAAATCAAAATTTGCGGATTGGTTCCATGTTAATAGCTGGCCAGCAACTTACACACCAACTG
ATGATTTTGAAAATGCGGAAAATGCAACTTATGATACTTTTGCCTTTACCCCACACATGCCAAAACTGAATACGGCTAAC
CCAGAGGTTGAAGCATATCTTTTAAAAGTAGCTGATTATTGGATTTCTGAGTTTGATATTGATGCTTGGCGCTTGGATGT
GGCAGATGAAGTAGACCATACTTTCTGGAAAAAATTCCGCACAACTTGTGACGCGGCAAAAGATGATTTTTATATTTTAG
GGGAAGTTTGGCACTCTGCTCAACCTTGGTTAGTTGGCGACGAATTCTCTGCTGTTATGAATTATGCCTATACCGATGCG
ATTAAAGATGGTTTGATTACTAAGAAAATTTCTTTAGAACAAATGGTCTCTAATATTAATACGCAACTTACATTATATCG
TAAGCAAGTCAATCAAATGATGTTTAATGTTTTAGATTCTCATGATACCCCAAGAATTTTGACTGTTGCTAAAAATAATA
AAAACCTGATGAAATTGGTTGAAACGTTCACCTTCATGCAACCGGGTGTACCTTGTATTTATTATGGAGATGAGTATGCA
GTGACAGGCGGAATGGACCCTGAGTGTCGTAAGGTGATGCCTTGGGAAGAAAAAGATCAAGATTTAGAGATGTTTGAATT
CTTTAAAAATCTGATTGCTCTGCGCAAACAATATCAAGGAATTTTAAGTGATTCAGAAGTAGAATGGAAAATCGTAGATT
CAGAAAATGGCATTGTGAAATTTGAACGTGGAACTTTGAAAGCTATTTTCAATATTGGCGAAAAACCGGCTTATTTTTCG
GCTGATGAAGTGGTTTTCTCTAATTTGGTTGATCAAAATAATATTTTACAATATGGTTTTGTAATCTATAAATAG

Upstream 100 bases:

>100_bases
CGAAAAGTGAGTATAAATGGACTGACTTTTCACTGTCCGTGAGATTAATTGAGAATAGTTTGAGACAGTACAGCCATCAA
AAAAATTTGGAGGTTCTATT

Downstream 100 bases:

>100_bases
AAAATGAGTTCTGTCAGTTCAATGCAACCGAGGGGAGTCATTGAATCGGTGAATAAAGAAACTATCGGCTTTGCTGATAG
TTTTGTCAGTAAAAATTGCT

Product: neopullulanase

Products: alpha-D-glucose; beta-D-glucose; amylose(n-1) [C]

Alternate protein names: NA

Number of amino acids: Translated: 584; Mature: 584

Protein sequence:

>584_residues
MNKAAIYHRPESEFAYLYAKNLMHVRLQTQKSDVEKVELFYGDPYSWEGQDKPDTQYWASQKVEMKKYLSTNISDYFEAE
ITVATKRVDYLFVITGLDGEKVVYTDQGMFDYDEALVTQKYGAFRLPYFHESDRFKAPEWVKETVWYQIFPERFANGDTS
NDPEGTKAWNPQDVPDRQDFYGGDLQGVIDHLEHLTELGVNGIYFTPIFQAFSNHKYDTEDYMEIDKQFGDKELFKTLVK
EAHARGIKVMLDAVFNHIGDTSAQWQDVLKNQEKSKFADWFHVNSWPATYTPTDDFENAENATYDTFAFTPHMPKLNTAN
PEVEAYLLKVADYWISEFDIDAWRLDVADEVDHTFWKKFRTTCDAAKDDFYILGEVWHSAQPWLVGDEFSAVMNYAYTDA
IKDGLITKKISLEQMVSNINTQLTLYRKQVNQMMFNVLDSHDTPRILTVAKNNKNLMKLVETFTFMQPGVPCIYYGDEYA
VTGGMDPECRKVMPWEEKDQDLEMFEFFKNLIALRKQYQGILSDSEVEWKIVDSENGIVKFERGTLKAIFNIGEKPAYFS
ADEVVFSNLVDQNNILQYGFVIYK

Sequences:

>Translated_584_residues
MNKAAIYHRPESEFAYLYAKNLMHVRLQTQKSDVEKVELFYGDPYSWEGQDKPDTQYWASQKVEMKKYLSTNISDYFEAE
ITVATKRVDYLFVITGLDGEKVVYTDQGMFDYDEALVTQKYGAFRLPYFHESDRFKAPEWVKETVWYQIFPERFANGDTS
NDPEGTKAWNPQDVPDRQDFYGGDLQGVIDHLEHLTELGVNGIYFTPIFQAFSNHKYDTEDYMEIDKQFGDKELFKTLVK
EAHARGIKVMLDAVFNHIGDTSAQWQDVLKNQEKSKFADWFHVNSWPATYTPTDDFENAENATYDTFAFTPHMPKLNTAN
PEVEAYLLKVADYWISEFDIDAWRLDVADEVDHTFWKKFRTTCDAAKDDFYILGEVWHSAQPWLVGDEFSAVMNYAYTDA
IKDGLITKKISLEQMVSNINTQLTLYRKQVNQMMFNVLDSHDTPRILTVAKNNKNLMKLVETFTFMQPGVPCIYYGDEYA
VTGGMDPECRKVMPWEEKDQDLEMFEFFKNLIALRKQYQGILSDSEVEWKIVDSENGIVKFERGTLKAIFNIGEKPAYFS
ADEVVFSNLVDQNNILQYGFVIYK
>Mature_584_residues
MNKAAIYHRPESEFAYLYAKNLMHVRLQTQKSDVEKVELFYGDPYSWEGQDKPDTQYWASQKVEMKKYLSTNISDYFEAE
ITVATKRVDYLFVITGLDGEKVVYTDQGMFDYDEALVTQKYGAFRLPYFHESDRFKAPEWVKETVWYQIFPERFANGDTS
NDPEGTKAWNPQDVPDRQDFYGGDLQGVIDHLEHLTELGVNGIYFTPIFQAFSNHKYDTEDYMEIDKQFGDKELFKTLVK
EAHARGIKVMLDAVFNHIGDTSAQWQDVLKNQEKSKFADWFHVNSWPATYTPTDDFENAENATYDTFAFTPHMPKLNTAN
PEVEAYLLKVADYWISEFDIDAWRLDVADEVDHTFWKKFRTTCDAAKDDFYILGEVWHSAQPWLVGDEFSAVMNYAYTDA
IKDGLITKKISLEQMVSNINTQLTLYRKQVNQMMFNVLDSHDTPRILTVAKNNKNLMKLVETFTFMQPGVPCIYYGDEYA
VTGGMDPECRKVMPWEEKDQDLEMFEFFKNLIALRKQYQGILSDSEVEWKIVDSENGIVKFERGTLKAIFNIGEKPAYFS
ADEVVFSNLVDQNNILQYGFVIYK

Specific function: Hydrolyzes beta-cyclodextrin to maltose and glucose, soluble starch to maltose and glucose, and pullulan to panose with trace amounts of maltose and glucose. It is also able to hydrolyze acarbose. Can also exhibit a transglycosylation activity transferrin

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family. BbmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=228, Percent_Identity=24.5614035087719, Blast_Score=82, Evalue=2e-15,
Organism=Escherichia coli, GI1786604, Length=443, Percent_Identity=31.3769751693002, Blast_Score=194, Evalue=2e-50,
Organism=Escherichia coli, GI1790687, Length=417, Percent_Identity=25.6594724220624, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1789995, Length=466, Percent_Identity=24.2489270386266, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6322245, Length=228, Percent_Identity=25.4385964912281, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6321726, Length=332, Percent_Identity=23.4939759036145, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324416, Length=395, Percent_Identity=22.5316455696203, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322241, Length=289, Percent_Identity=24.2214532871972, Blast_Score=68, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6322021, Length=289, Percent_Identity=24.2214532871972, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI221330053, Length=408, Percent_Identity=24.0196078431373, Blast_Score=83, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24586587, Length=234, Percent_Identity=26.9230769230769, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24586599, Length=418, Percent_Identity=23.9234449760766, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24583749, Length=236, Percent_Identity=26.6949152542373, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24583747, Length=236, Percent_Identity=26.6949152542373, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24583745, Length=241, Percent_Identity=25.7261410788382, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24586593, Length=458, Percent_Identity=21.8340611353712, Blast_Score=71, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24586597, Length=225, Percent_Identity=25.7777777777778, Blast_Score=70, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24586591, Length=224, Percent_Identity=25, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24586589, Length=283, Percent_Identity=25.0883392226148, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI45549022, Length=225, Percent_Identity=26.6666666666667, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N [H]

EC number: 3.2.1.20 [C]

Molecular weight: Translated: 68063; Mature: 68063

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKAAIYHRPESEFAYLYAKNLMHVRLQTQKSDVEKVELFYGDPYSWEGQDKPDTQYWAS
CCCCEEEECCCCCEEHHHHHCEEEEEEECCCCCCEEEEEEECCCCCCCCCCCCCHHHHHH
QKVEMKKYLSTNISDYFEAEITVATKRVDYLFVITGLDGEKVVYTDQGMFDYDEALVTQK
HHHHHHHHHHCCCHHHHEEEEEEEEEEEEEEEEEECCCCCEEEEECCCCCCHHHHHHHHC
YGAFRLPYFHESDRFKAPEWVKETVWYQIFPERFANGDTSNDPEGTKAWNPQDVPDRQDF
CCCEECCCCCCCCCCCCHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
YGGDLQGVIDHLEHLTELGVNGIYFTPIFQAFSNHKYDTEDYMEIDKQFGDKELFKTLVK
CCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHH
EAHARGIKVMLDAVFNHIGDTSAQWQDVLKNQEKSKFADWFHVNSWPATYTPTDDFENAE
HHHHCCHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHCEEECCCCCCCCCCCCCCCCCC
NATYDTFAFTPHMPKLNTANPEVEAYLLKVADYWISEFDIDAWRLDVADEVDHTFWKKFR
CCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHH
TTCDAAKDDFYILGEVWHSAQPWLVGDEFSAVMNYAYTDAIKDGLITKKISLEQMVSNIN
HHHCCCCCCEEEEEEHHCCCCCCEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
TQLTLYRKQVNQMMFNVLDSHDTPRILTVAKNNKNLMKLVETFTFMQPGVPCIYYGDEYA
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCEEEECCEEE
VTGGMDPECRKVMPWEEKDQDLEMFEFFKNLIALRKQYQGILSDSEVEWKIVDSENGIVK
EECCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEE
FERGTLKAIFNIGEKPAYFSADEVVFSNLVDQNNILQYGFVIYK
ECCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEECEEEEC
>Mature Secondary Structure
MNKAAIYHRPESEFAYLYAKNLMHVRLQTQKSDVEKVELFYGDPYSWEGQDKPDTQYWAS
CCCCEEEECCCCCEEHHHHHCEEEEEEECCCCCCEEEEEEECCCCCCCCCCCCCHHHHHH
QKVEMKKYLSTNISDYFEAEITVATKRVDYLFVITGLDGEKVVYTDQGMFDYDEALVTQK
HHHHHHHHHHCCCHHHHEEEEEEEEEEEEEEEEEECCCCCEEEEECCCCCCHHHHHHHHC
YGAFRLPYFHESDRFKAPEWVKETVWYQIFPERFANGDTSNDPEGTKAWNPQDVPDRQDF
CCCEECCCCCCCCCCCCHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
YGGDLQGVIDHLEHLTELGVNGIYFTPIFQAFSNHKYDTEDYMEIDKQFGDKELFKTLVK
CCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHH
EAHARGIKVMLDAVFNHIGDTSAQWQDVLKNQEKSKFADWFHVNSWPATYTPTDDFENAE
HHHHCCHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHCEEECCCCCCCCCCCCCCCCCC
NATYDTFAFTPHMPKLNTANPEVEAYLLKVADYWISEFDIDAWRLDVADEVDHTFWKKFR
CCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHH
TTCDAAKDDFYILGEVWHSAQPWLVGDEFSAVMNYAYTDAIKDGLITKKISLEQMVSNIN
HHHCCCCCCEEEEEEHHCCCCCCEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
TQLTLYRKQVNQMMFNVLDSHDTPRILTVAKNNKNLMKLVETFTFMQPGVPCIYYGDEYA
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCEEEECCEEE
VTGGMDPECRKVMPWEEKDQDLEMFEFFKNLIALRKQYQGILSDSEVEWKIVDSENGIVK
EECCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEE
FERGTLKAIFNIGEKPAYFSADEVVFSNLVDQNNILQYGFVIYK
ECCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEECEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): {} [C]

Substrates: Maltose; H2O; 1,4-alpha-D-glucan [C]

Specific reaction: Maltose + H2O = 2 alpha-D-glucose H2O + 1,4-alpha-D-glucan = beta-D-glucose + amylose(n-1) [C]

General reaction: O-Glycosyl bond hydrolysis; Exohydrolysis [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]