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The map label for this gene is amd [H]

Identifier: 125623522

GI number: 125623522

Start: 660769

End: 661965

Strand: Reverse

Name: amd [H]

Synonym: llmg_0664

Alternate gene names: 125623522

Gene position: 661965-660769 (Counterclockwise)

Preceding gene: 125623525

Following gene: 125623516

Centisome position: 26.17

GC content: 35.67

Gene sequence:

>1197_bases
ATGAAACTACTTAATAATTTGTTGACTAGCTTAACACAATATGAGAATGAAATGATTCAAATTAGGCGTCATCTTCATCA
ATACCCAGAAATTTCTTTTCAAGAAAAAGAAACATTTAAGTATATTATGGGCTTTTATAAAGAACTTGACTGTGAACCAA
AACTTATCGGAAAGGGATTTGGAATCATCGTTGATATTGAAGGTGGAAAATCCGGAAAAACTCTTGCGTTGAGAGCAGAC
TTTGATGCTCTTGCTATTTTTGAAGATAATGATTTAAGTTTTAAATCGGTCAATCCAGGAGTAATGCATGCCTGCGGACA
CGATGCACATACTGCATATTTGATGGTACTTGCTAGAGAACTAGTAAAAATAAAACAAGAACTTCCGGGAAGAGTTAGAA
TTGTCCATCAACCAGCAGAGGAAGTATCTCCTGGTGGTGCCAAAAGTATGATTAAAGCAGGAGCTCTTGACGGTGTCGAT
AATATGATCGGGGTTCATGTGATGACTACAATTAAAACAGGGGTCATTGCCTATCATAATAAAGAAACTCAAACGGGACG
TTCTAATTTCACCATTACAATCAAAGGAAACGGAGGTCACGCTTCTATGCCACAATTAAGTAATGATGCAATAGTAGCCG
CTAGTTACTTTGTAACTGAACTCCAAACAGTAATTTCAAGAAGAATAGATCCATTTGACATGGGTACTGTAACTATTGGA
TCATTTGATGGAGCAGGAAGTTTTAATGCTATACAAGATAAAGTAGTGTTAAAAGGGGATGTTCGGATGATGAAAGAAAC
AACTAGAAAAGTCATCCGAGACCAAGTGAAACAAATTGCTAAAGGCGTTGGAGTAACCTTTGGGGTTGAAGTGATTGTAG
ATTATGACGATAACTATCCTGTACTCTTTAATTCAGAAAATCTCACTCACTTTGTAGTTGACAGCTTAAAAGATCAGAAT
ATTTCAGAAGTCAATAATATAGTCGATTTAGGACCACAAAATCCTTCTGAAGATTTTTCATATTATGGGCAAGTTGTACC
AAGTACTTTCTTTTACATTGGTGCACAGCCTGAAGACGGCGGAAATTATCCCCATCATAGCCCTTTATTTAAAATGAATG
AGAAAAGTATTTTAATCGCGGCTAAAGCAGTTGCAACTGTAACTATAAACTATCTATTTTTCAATAAAAAAATGTAG

Upstream 100 bases:

>100_bases
AGCTTTCTAATGCTTAGTGCTTTAAGATTAGGATAGCACGACTTATTTATTTTCCAATGAAATTAACTAGCAATTCGGGT
AGAAAGTTTAGAGGTATCAT

Downstream 100 bases:

>100_bases
ATTTACAAATAGCAAGATTTGTAGCTAAAGTTGCTCCTTCGTTATTTAATAAACTTATAAAAATATTTCTATCCTATGTC
CAGAAAACGACCATTTTCTG

Product: amidase/aminoacylase

Products: carboxylate; L-amino acid

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MKLLNNLLTSLTQYENEMIQIRRHLHQYPEISFQEKETFKYIMGFYKELDCEPKLIGKGFGIIVDIEGGKSGKTLALRAD
FDALAIFEDNDLSFKSVNPGVMHACGHDAHTAYLMVLARELVKIKQELPGRVRIVHQPAEEVSPGGAKSMIKAGALDGVD
NMIGVHVMTTIKTGVIAYHNKETQTGRSNFTITIKGNGGHASMPQLSNDAIVAASYFVTELQTVISRRIDPFDMGTVTIG
SFDGAGSFNAIQDKVVLKGDVRMMKETTRKVIRDQVKQIAKGVGVTFGVEVIVDYDDNYPVLFNSENLTHFVVDSLKDQN
ISEVNNIVDLGPQNPSEDFSYYGQVVPSTFFYIGAQPEDGGNYPHHSPLFKMNEKSILIAAKAVATVTINYLFFNKKM

Sequences:

>Translated_398_residues
MKLLNNLLTSLTQYENEMIQIRRHLHQYPEISFQEKETFKYIMGFYKELDCEPKLIGKGFGIIVDIEGGKSGKTLALRAD
FDALAIFEDNDLSFKSVNPGVMHACGHDAHTAYLMVLARELVKIKQELPGRVRIVHQPAEEVSPGGAKSMIKAGALDGVD
NMIGVHVMTTIKTGVIAYHNKETQTGRSNFTITIKGNGGHASMPQLSNDAIVAASYFVTELQTVISRRIDPFDMGTVTIG
SFDGAGSFNAIQDKVVLKGDVRMMKETTRKVIRDQVKQIAKGVGVTFGVEVIVDYDDNYPVLFNSENLTHFVVDSLKDQN
ISEVNNIVDLGPQNPSEDFSYYGQVVPSTFFYIGAQPEDGGNYPHHSPLFKMNEKSILIAAKAVATVTINYLFFNKKM
>Mature_398_residues
MKLLNNLLTSLTQYENEMIQIRRHLHQYPEISFQEKETFKYIMGFYKELDCEPKLIGKGFGIIVDIEGGKSGKTLALRAD
FDALAIFEDNDLSFKSVNPGVMHACGHDAHTAYLMVLARELVKIKQELPGRVRIVHQPAEEVSPGGAKSMIKAGALDGVD
NMIGVHVMTTIKTGVIAYHNKETQTGRSNFTITIKGNGGHASMPQLSNDAIVAASYFVTELQTVISRRIDPFDMGTVTIG
SFDGAGSFNAIQDKVVLKGDVRMMKETTRKVIRDQVKQIAKGVGVTFGVEVIVDYDDNYPVLFNSENLTHFVVDSLKDQN
ISEVNNIVDLGPQNPSEDFSYYGQVVPSTFFYIGAQPEDGGNYPHHSPLFKMNEKSILIAAKAVATVTINYLFFNKKM

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=460, Percent_Identity=25.4347826086956, Blast_Score=95, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: 3.5.1.14

Molecular weight: Translated: 44099; Mature: 44099

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLNNLLTSLTQYENEMIQIRRHLHQYPEISFQEKETFKYIMGFYKELDCEPKLIGKGF
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEECCCC
GIIVDIEGGKSGKTLALRADFDALAIFEDNDLSFKSVNPGVMHACGHDAHTAYLMVLARE
EEEEEECCCCCCCEEEEEECCCEEEEEECCCCCEECCCCCHHHHCCCCHHHHHHHHHHHH
LVKIKQELPGRVRIVHQPAEEVSPGGAKSMIKAGALDGVDNMIGVHVMTTIKTGVIAYHN
HHHHHHHCCCEEEEEECCHHHCCCCCHHHHHHHCCCCCCHHHHHHHEEEEHHCCEEEEEC
KETQTGRSNFTITIKGNGGHASMPQLSNDAIVAASYFVTELQTVISRRIDPFDMGTVTIG
CCCCCCCCEEEEEEEECCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCEEEEE
SFDGAGSFNAIQDKVVLKGDVRMMKETTRKVIRDQVKQIAKGVGVTFGVEVIVDYDDNYP
CCCCCCCCCHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEECCCCC
VLFNSENLTHFVVDSLKDQNISEVNNIVDLGPQNPSEDFSYYGQVVPSTFFYIGAQPEDG
EEECCCCCHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCC
GNYPHHSPLFKMNEKSILIAAKAVATVTINYLFFNKKM
CCCCCCCCCEEECCCEEEEEEEEEEEEEEEEEEEECCC
>Mature Secondary Structure
MKLLNNLLTSLTQYENEMIQIRRHLHQYPEISFQEKETFKYIMGFYKELDCEPKLIGKGF
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEECCCC
GIIVDIEGGKSGKTLALRADFDALAIFEDNDLSFKSVNPGVMHACGHDAHTAYLMVLARE
EEEEEECCCCCCCEEEEEECCCEEEEEECCCCCEECCCCCHHHHCCCCHHHHHHHHHHHH
LVKIKQELPGRVRIVHQPAEEVSPGGAKSMIKAGALDGVDNMIGVHVMTTIKTGVIAYHN
HHHHHHHCCCEEEEEECCHHHCCCCCHHHHHHHCCCCCCHHHHHHHEEEEHHCCEEEEEC
KETQTGRSNFTITIKGNGGHASMPQLSNDAIVAASYFVTELQTVISRRIDPFDMGTVTIG
CCCCCCCCEEEEEEEECCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCEEEEE
SFDGAGSFNAIQDKVVLKGDVRMMKETTRKVIRDQVKQIAKGVGVTFGVEVIVDYDDNYP
CCCCCCCCCHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEECCCCC
VLFNSENLTHFVVDSLKDQNISEVNNIVDLGPQNPSEDFSYYGQVVPSTFFYIGAQPEDG
EEECCCCCHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCC
GNYPHHSPLFKMNEKSILIAAKAVATVTINYLFFNKKM
CCCCCCCCCEEECCCEEEEEEEEEEEEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-acyl-L-amino acid; H2O

Specific reaction: An N-acyl-L-amino acid + H2O = a carboxylate + an L-amino acid

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]