| Definition | Lactococcus lactis subsp. cremoris MG1363, complete genome. |
|---|---|
| Accession | NC_009004 |
| Length | 2,529,478 |
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The map label for this gene is pfl [H]
Identifier: 125623489
GI number: 125623489
Start: 618464
End: 620827
Strand: Direct
Name: pfl [H]
Synonym: llmg_0629
Alternate gene names: 125623489
Gene position: 618464-620827 (Clockwise)
Preceding gene: 125623488
Following gene: 125623490
Centisome position: 24.45
GC content: 38.16
Gene sequence:
>2364_bases ATGAAAACCGAAGTTACGGAAAATATCTTTGAACAAGCTTGGGATGGTTTTAAAGGAACTAACTGGCGCGATAAAGCAAG CGTTACTCGCTTTGTACAAGAAAACTACAAACCATATGATGGTGATGAAAGCTTTCTTGCTGGGCCAACAGAACGTACAC TTAAAGTAAAGAAAATTATTGAAGATACAAAAAATCACTACGAAGAAGTAGGATTTCCCTTTGATACTGACCGCGTAACC TCTATCGATAAAATTCCTGCTGGATATATTGATGCTAATGATAAAGAACTTGAACTCATCTATGGGATGCAAAATAGCGA ACTTTTCCGCTTAAACTTCATGCCAAGAGGTGGTCTTCGTGTTGCTGAAAAGATTTTGACAGAACACGGTCTTTCAGTTG ACCCAGGTTTGCATGATGTTTTGTCACAAACAATGACTTCTGTAAATGATGGAATCTTCCGTGCTTATACTTCAGCAATT CGTAAAGCACGTCACGCTCACACTGTAACAGGTTTGCCTGATGCATACTCTCGTGGACGTATCATCGGGGTATATGCACG TCTTGCTCTTTATGGAGCTGACTACCTTATGAAGGAAAAAGCAAAAGAATGGGATGCAATCACTGAAATTAATGATGATA ACATTCGTCTTAAAGAAGAAATTAACATGCAATACCAAGCTTTGCAAGAAGTTGTAAACTTTGGTGCTTTGTATGGTCTT GACGTTTCTCGTCCAGCGATGAACGTAAAAGAAGCAATCCAATGGGTTAATATTGCATACATGGCAGTTTGTCGTGTTAT CAATGGTGCTGCAACTTCACTTGGACGTGTGCCAATCGTTCTTGACATCTTTGCAGAACGTGACCTTGCTCGTGGAACAT TTACTGAGCAAGAAATCCAAGAATTTGTTGATGATTTCATTTTAAAACTTCGTACAATGAAATTTGCTCGTGCTGCTGCT TATGATGAACTTTATTCTGGTGACCCCACGTTCATCACAACATCTATGGCTGGTATGGGTAATGACGGACGCCACCGTGT CACTAAAATGGACTATCGTTTCTTGAACACACTTGATACAATCGGAAATGCTCCAGAACCAAACTTGACAGTTCTTTGGG ACTCTAAACTCCCATATTCATTCAAACGTTATTCAATGTCTATGAGTCACAAACACTCATCTATCCAATATGAAGGTGTT GAAACAATGGCTAAAGATGGATATGGCGAAATGTCATGTATCTCTTGTTGTGTCTCACCACTTGACCCAGAAAATGAAGA AGGACGTCATAATCTCCAATACTTTGGTGCGCGTGTAAACGTCTTGAAAGCAATGTTGACTGGTTTGAACGGTGGTTACG ATGACGTTCATAAAGATTATAAAGTATTCGATATTGAACCTGTTCGTGATGAAATTCTTGACTATGATACAGTTATGGAA AACTTCGACAAATCACTCAACTGGTTGACAGATACTTATGTTGATGCAATGAATATCATTCACTACATGACTGACAAATA TAACTATGAAGCAGTTCAAATGGCCTTCTTGCCTACTAAAGTTCGTGCTAACATGGGATTTGGTATCTGTGGTTTCGCAA ATACAGTTGATTCACTTTCAGCGATTAAATATGCTAAAGTTAAAACTTTGCGTGATGAAAATGGCTACATCTACGATTAT GAAGTAGAAGGTGACTTCCCACGTTATGGTGAAGATGATGACCGTGCTGATGATATCGCTAAACTTGTCATGAAAATGTA CCATGAAAAATTAGCTTCACACAAACTTTACAAAAATGCTGAAGCTACTGTTTCACTTTTGACAATCACATCTAACGTTG CTTACTCTAAACAAACTGGTAACTCTCCAGTTCATAAAGGAGTATTCCTCAATGAAGATGGTACAGTCAACAAATCTAAA CTTGAATTCTTCTCACCAGGTGCTAACCCATCTAACAAAGCTAAAGGTGGATGGTTGCAAAATCTTCGTTCATTAGCTAA ATTGGAATTCAAAGATGCAAATGACGGTATTTCATTAACTACTCAAGTTTCTCCTCGTGCACTTGGTAAAACTCGTGATG AACAAGTAGATAACTTGGTTCAAATTCTTGATGGATACTTCACACCAGGAGCTTTGATTAATGGTACTGAATTTGCAGGT CAACACGTTAACTTGAACGTTATGGACCTTAAAGATGTTTACGATAAAATCATGCGTGGTGAAGATGTTATCGTTCGTAT CTCTGGATACTGTGTTAACACTAAATACCTCACACCTGAACAAAAACAAGAATTGACTGAACGTGTCTTCCATGAAGTAC TTTCAAATGATGATGAAGAAGTAATGCACACTTCAAATATCTAA
Upstream 100 bases:
>100_bases AAAGAATCTGTAATTTCTCTTGAATTCTGTTTGCTATTATCAAACTGTATGATATAATGAAGTTGTAATTTGAAACAGAA AGAACAAAGGAGATTTCAAA
Downstream 100 bases:
>100_bases TTCTTAGTATTAAAAAATATAAAGTCTGTCAGTTCTACTGACAGACTTTTTTTCTATAAATTAATTATAATAGTTAAAAA CTATTATTTTTAGTTTAAGA
Product: formate acetyltransferase
Products: NA
Alternate protein names: Pyruvate formate-lyase [H]
Number of amino acids: Translated: 787; Mature: 787
Protein sequence:
>787_residues MKTEVTENIFEQAWDGFKGTNWRDKASVTRFVQENYKPYDGDESFLAGPTERTLKVKKIIEDTKNHYEEVGFPFDTDRVT SIDKIPAGYIDANDKELELIYGMQNSELFRLNFMPRGGLRVAEKILTEHGLSVDPGLHDVLSQTMTSVNDGIFRAYTSAI RKARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMKEKAKEWDAITEINDDNIRLKEEINMQYQALQEVVNFGALYGL DVSRPAMNVKEAIQWVNIAYMAVCRVINGAATSLGRVPIVLDIFAERDLARGTFTEQEIQEFVDDFILKLRTMKFARAAA YDELYSGDPTFITTSMAGMGNDGRHRVTKMDYRFLNTLDTIGNAPEPNLTVLWDSKLPYSFKRYSMSMSHKHSSIQYEGV ETMAKDGYGEMSCISCCVSPLDPENEEGRHNLQYFGARVNVLKAMLTGLNGGYDDVHKDYKVFDIEPVRDEILDYDTVME NFDKSLNWLTDTYVDAMNIIHYMTDKYNYEAVQMAFLPTKVRANMGFGICGFANTVDSLSAIKYAKVKTLRDENGYIYDY EVEGDFPRYGEDDDRADDIAKLVMKMYHEKLASHKLYKNAEATVSLLTITSNVAYSKQTGNSPVHKGVFLNEDGTVNKSK LEFFSPGANPSNKAKGGWLQNLRSLAKLEFKDANDGISLTTQVSPRALGKTRDEQVDNLVQILDGYFTPGALINGTEFAG QHVNLNVMDLKDVYDKIMRGEDVIVRISGYCVNTKYLTPEQKQELTERVFHEVLSNDDEEVMHTSNI
Sequences:
>Translated_787_residues MKTEVTENIFEQAWDGFKGTNWRDKASVTRFVQENYKPYDGDESFLAGPTERTLKVKKIIEDTKNHYEEVGFPFDTDRVT SIDKIPAGYIDANDKELELIYGMQNSELFRLNFMPRGGLRVAEKILTEHGLSVDPGLHDVLSQTMTSVNDGIFRAYTSAI RKARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMKEKAKEWDAITEINDDNIRLKEEINMQYQALQEVVNFGALYGL DVSRPAMNVKEAIQWVNIAYMAVCRVINGAATSLGRVPIVLDIFAERDLARGTFTEQEIQEFVDDFILKLRTMKFARAAA YDELYSGDPTFITTSMAGMGNDGRHRVTKMDYRFLNTLDTIGNAPEPNLTVLWDSKLPYSFKRYSMSMSHKHSSIQYEGV ETMAKDGYGEMSCISCCVSPLDPENEEGRHNLQYFGARVNVLKAMLTGLNGGYDDVHKDYKVFDIEPVRDEILDYDTVME NFDKSLNWLTDTYVDAMNIIHYMTDKYNYEAVQMAFLPTKVRANMGFGICGFANTVDSLSAIKYAKVKTLRDENGYIYDY EVEGDFPRYGEDDDRADDIAKLVMKMYHEKLASHKLYKNAEATVSLLTITSNVAYSKQTGNSPVHKGVFLNEDGTVNKSK LEFFSPGANPSNKAKGGWLQNLRSLAKLEFKDANDGISLTTQVSPRALGKTRDEQVDNLVQILDGYFTPGALINGTEFAG QHVNLNVMDLKDVYDKIMRGEDVIVRISGYCVNTKYLTPEQKQELTERVFHEVLSNDDEEVMHTSNI >Mature_787_residues MKTEVTENIFEQAWDGFKGTNWRDKASVTRFVQENYKPYDGDESFLAGPTERTLKVKKIIEDTKNHYEEVGFPFDTDRVT SIDKIPAGYIDANDKELELIYGMQNSELFRLNFMPRGGLRVAEKILTEHGLSVDPGLHDVLSQTMTSVNDGIFRAYTSAI RKARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMKEKAKEWDAITEINDDNIRLKEEINMQYQALQEVVNFGALYGL DVSRPAMNVKEAIQWVNIAYMAVCRVINGAATSLGRVPIVLDIFAERDLARGTFTEQEIQEFVDDFILKLRTMKFARAAA YDELYSGDPTFITTSMAGMGNDGRHRVTKMDYRFLNTLDTIGNAPEPNLTVLWDSKLPYSFKRYSMSMSHKHSSIQYEGV ETMAKDGYGEMSCISCCVSPLDPENEEGRHNLQYFGARVNVLKAMLTGLNGGYDDVHKDYKVFDIEPVRDEILDYDTVME NFDKSLNWLTDTYVDAMNIIHYMTDKYNYEAVQMAFLPTKVRANMGFGICGFANTVDSLSAIKYAKVKTLRDENGYIYDY EVEGDFPRYGEDDDRADDIAKLVMKMYHEKLASHKLYKNAEATVSLLTITSNVAYSKQTGNSPVHKGVFLNEDGTVNKSK LEFFSPGANPSNKAKGGWLQNLRSLAKLEFKDANDGISLTTQVSPRALGKTRDEQVDNLVQILDGYFTPGALINGTEFAG QHVNLNVMDLKDVYDKIMRGEDVIVRISGYCVNTKYLTPEQKQELTERVFHEVLSNDDEEVMHTSNI
Specific function: Glucose metabolism (nonoxidative conversion). [C]
COG id: COG1882
COG function: function code C; Pyruvate-formate lyase
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 pyruvate formate lyase domain [H]
Homologues:
Organism=Escherichia coli, GI1787131, Length=774, Percent_Identity=42.6356589147287, Blast_Score=606, Evalue=1e-174, Organism=Escherichia coli, GI48994926, Length=788, Percent_Identity=40.8629441624365, Blast_Score=588, Evalue=1e-169,
Paralogues:
None
Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR005949 - InterPro: IPR001150 - InterPro: IPR019777 - InterPro: IPR004184 [H]
Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]
EC number: =2.3.1.54 [H]
Molecular weight: Translated: 89108; Mature: 89108
Theoretical pI: Translated: 4.93; Mature: 4.93
Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.8 %Cys (Translated Protein) 3.4 %Met (Translated Protein) 4.2 %Cys+Met (Translated Protein) 0.8 %Cys (Mature Protein) 3.4 %Met (Mature Protein) 4.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKTEVTENIFEQAWDGFKGTNWRDKASVTRFVQENYKPYDGDESFLAGPTERTLKVKKII CCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH EDTKNHYEEVGFPFDTDRVTSIDKIPAGYIDANDKELELIYGMQNSELFRLNFMPRGGLR HHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCHH VAEKILTEHGLSVDPGLHDVLSQTMTSVNDGIFRAYTSAIRKARHAHTVTGLPDAYSRGR HHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCHHHCCC IIGVYARLALYGADYLMKEKAKEWDAITEINDDNIRLKEEINMQYQALQEVVNFGALYGL EEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHCHHHHHHHHHHHHHHHCCC DVSRPAMNVKEAIQWVNIAYMAVCRVINGAATSLGRVPIVLDIFAERDLARGTFTEQEIQ CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHCCCHHCCCCCHHHHH EFVDDFILKLRTMKFARAAAYDELYSGDPTFITTSMAGMGNDGRHRVTKMDYRFLNTLDT HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHH IGNAPEPNLTVLWDSKLPYSFKRYSMSMSHKHSSIQYEGVETMAKDGYGEMSCISCCVSP HCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCEEECCHHHHHHCCCCCHHHHHHHCCC LDPENEEGRHNLQYFGARVNVLKAMLTGLNGGYDDVHKDYKVFDIEPVRDEILDYDTVME CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEECCHHHHHHHHHHHHHH NFDKSLNWLTDTYVDAMNIIHYMTDKYNYEAVQMAFLPTKVRANMGFGICGFANTVDSLS HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHHCCCCCEECCHHHHHHHHH AIKYAKVKTLRDENGYIYDYEVEGDFPRYGEDDDRADDIAKLVMKMYHEKLASHKLYKNA HHHHHHHHEEECCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC EATVSLLTITSNVAYSKQTGNSPVHKGVFLNEDGTVNKSKLEFFSPGANPSNKAKGGWLQ CCEEEEEEEECCCEECCCCCCCCCCCCEEECCCCCCCCCHHEEECCCCCCCCCCCCHHHH NLRSLAKLEFKDANDGISLTTQVSPRALGKTRDEQVDNLVQILDGYFTPGALINGTEFAG HHHHHHHEECCCCCCCEEEEEECCCHHHCCCHHHHHHHHHHHHHCCCCCCCEECCHHHCC QHVNLNVMDLKDVYDKIMRGEDVIVRISGYCVNTKYLTPEQKQELTERVFHEVLSNDDEE CEEEEEEEEHHHHHHHHHCCCCEEEEEECEEEECCCCCCHHHHHHHHHHHHHHHCCCCHH VMHTSNI HHHCCCC >Mature Secondary Structure MKTEVTENIFEQAWDGFKGTNWRDKASVTRFVQENYKPYDGDESFLAGPTERTLKVKKII CCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH EDTKNHYEEVGFPFDTDRVTSIDKIPAGYIDANDKELELIYGMQNSELFRLNFMPRGGLR HHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCHH VAEKILTEHGLSVDPGLHDVLSQTMTSVNDGIFRAYTSAIRKARHAHTVTGLPDAYSRGR HHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCHHHCCC IIGVYARLALYGADYLMKEKAKEWDAITEINDDNIRLKEEINMQYQALQEVVNFGALYGL EEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHCHHHHHHHHHHHHHHHCCC DVSRPAMNVKEAIQWVNIAYMAVCRVINGAATSLGRVPIVLDIFAERDLARGTFTEQEIQ CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHCCCHHCCCCCHHHHH EFVDDFILKLRTMKFARAAAYDELYSGDPTFITTSMAGMGNDGRHRVTKMDYRFLNTLDT HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHH IGNAPEPNLTVLWDSKLPYSFKRYSMSMSHKHSSIQYEGVETMAKDGYGEMSCISCCVSP HCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCEEECCHHHHHHCCCCCHHHHHHHCCC LDPENEEGRHNLQYFGARVNVLKAMLTGLNGGYDDVHKDYKVFDIEPVRDEILDYDTVME CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEECCHHHHHHHHHHHHHH NFDKSLNWLTDTYVDAMNIIHYMTDKYNYEAVQMAFLPTKVRANMGFGICGFANTVDSLS HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHHCCCCCEECCHHHHHHHHH AIKYAKVKTLRDENGYIYDYEVEGDFPRYGEDDDRADDIAKLVMKMYHEKLASHKLYKNA HHHHHHHHEEECCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC EATVSLLTITSNVAYSKQTGNSPVHKGVFLNEDGTVNKSKLEFFSPGANPSNKAKGGWLQ CCEEEEEEEECCCEECCCCCCCCCCCCEEECCCCCCCCCHHEEECCCCCCCCCCCCHHHH NLRSLAKLEFKDANDGISLTTQVSPRALGKTRDEQVDNLVQILDGYFTPGALINGTEFAG HHHHHHHEECCCCCCCEEEEEECCCHHHCCCHHHHHHHHHHHHHCCCCCCCEECCHHHCC QHVNLNVMDLKDVYDKIMRGEDVIVRISGYCVNTKYLTPEQKQELTERVFHEVLSNDDEE CEEEEEEEEHHHHHHHHHCCCCEEEEEECEEEECCCCCCHHHHHHHHHHHHHHHCCCCHH VMHTSNI HHHCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9294449; 11337471 [H]