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The map label for this gene is miaA

Identifier: 125623452

GI number: 125623452

Start: 580430

End: 581314

Strand: Direct

Name: miaA

Synonym: llmg_0591

Alternate gene names: 125623452

Gene position: 580430-581314 (Clockwise)

Preceding gene: 125623451

Following gene: 125623453

Centisome position: 22.95

GC content: 37.18

Gene sequence:

>885_bases
ATGAAAAATAATAAAGTTTTGGTTGTTGTTGGACCAACTGCGGTGGGTAAAACGGCACTAGGCATTGATTTAGCTATAAA
AATGAATGGTGAAATTATTTCTGGTGATTCACAACAAGTGTATCAGGGATTAGATATTGGGACAGCGAAAGTAACAAAAG
CTGAACAAGCCTTAGCAGTTCATCATTTGATTGATGTTCGAAAATGGACAGAGAATTTTTCGGTTCATGATTTTGTGATG
GAAGCCAATCGATTGATTGAGGAGATTATTGAACGTGGGAATGTCCCGATTATTGTTGGTGGGACAGGACTCTATATTCA
GTCACTGATAGAAGGTTACCATCTGGGAGGTCAAGAAAATCATCAAGCAATGATGGAACTTCGAGAAACATTATTGGCTT
TAACTGACGAAGAGCTCTTTGAAAAAGTTCTTAAATTAAATCCAAATTTTCCTGAATTGAATCGCAGACGAGCGATTCGC
TTTTTGGAGTTGCAAACTTTTGGTTCAACTGATGAAAATAGTGGCTCTGATTATAATTTTTTATTAATTGGCTTGAATGC
AGAGCGAAAAGTGCTTTATGAAAGAATTAATCAGCGGGTAGAGCAAATGATGAGCGAAGGGCTTTTGTCTGAGGCAAGAA
GTCTATTTGAAAAGGCACCCGATGCTCAAGCAGCAAAAGGAATTGGTTATAAAGAATTTTTCCCTTACTTTTCTGGCGAG
ATTAGTTTAGAAGATGCAGTCGAATTAGTCAAACGTAATTCCAGACGTTATGCTAAAAGGCAATTGACTTGGTTCAGAAA
TCGAATGGAAGTCGAATTTGAGGATGTCTTTTCAGAAACATACCCTGATTCAGTTTTTGAGAAAGTGACTCAATTTTTGA
ACTGA

Upstream 100 bases:

>100_bases
ACAGAATGGTCAGTAACTTTTTTCTGGTCAAAGCTTGAATTTGCCGATTATTTCTGAAATAATAGAGATTGGAAATCGTG
GGAGTGAGTTAAAGATATAA

Downstream 100 bases:

>100_bases
CTCATTTTCGTAAAAATGTTATAATGAAAATGATTGAAAAAGGACATTATATCTGACTGACAGACTTTAAATCAGTCAAC
TTATTTTGCTGACAAAAAGA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MKNNKVLVVVGPTAVGKTALGIDLAIKMNGEIISGDSQQVYQGLDIGTAKVTKAEQALAVHHLIDVRKWTENFSVHDFVM
EANRLIEEIIERGNVPIIVGGTGLYIQSLIEGYHLGGQENHQAMMELRETLLALTDEELFEKVLKLNPNFPELNRRRAIR
FLELQTFGSTDENSGSDYNFLLIGLNAERKVLYERINQRVEQMMSEGLLSEARSLFEKAPDAQAAKGIGYKEFFPYFSGE
ISLEDAVELVKRNSRRYAKRQLTWFRNRMEVEFEDVFSETYPDSVFEKVTQFLN

Sequences:

>Translated_294_residues
MKNNKVLVVVGPTAVGKTALGIDLAIKMNGEIISGDSQQVYQGLDIGTAKVTKAEQALAVHHLIDVRKWTENFSVHDFVM
EANRLIEEIIERGNVPIIVGGTGLYIQSLIEGYHLGGQENHQAMMELRETLLALTDEELFEKVLKLNPNFPELNRRRAIR
FLELQTFGSTDENSGSDYNFLLIGLNAERKVLYERINQRVEQMMSEGLLSEARSLFEKAPDAQAAKGIGYKEFFPYFSGE
ISLEDAVELVKRNSRRYAKRQLTWFRNRMEVEFEDVFSETYPDSVFEKVTQFLN
>Mature_294_residues
MKNNKVLVVVGPTAVGKTALGIDLAIKMNGEIISGDSQQVYQGLDIGTAKVTKAEQALAVHHLIDVRKWTENFSVHDFVM
EANRLIEEIIERGNVPIIVGGTGLYIQSLIEGYHLGGQENHQAMMELRETLLALTDEELFEKVLKLNPNFPELNRRRAIR
FLELQTFGSTDENSGSDYNFLLIGLNAERKVLYERINQRVEQMMSEGLLSEARSLFEKAPDAQAAKGIGYKEFFPYFSGE
ISLEDAVELVKRNSRRYAKRQLTWFRNRMEVEFEDVFSETYPDSVFEKVTQFLN

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=311, Percent_Identity=31.1897106109325, Blast_Score=135, Evalue=6e-32,
Organism=Escherichia coli, GI1790613, Length=286, Percent_Identity=32.8671328671329, Blast_Score=147, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI25144712, Length=311, Percent_Identity=29.5819935691318, Blast_Score=100, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6324848, Length=292, Percent_Identity=31.8493150684932, Blast_Score=150, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24649830, Length=342, Percent_Identity=33.9181286549708, Blast_Score=144, Evalue=7e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_LACLM (A2RIU4)

Other databases:

- EMBL:   AM406671
- RefSeq:   YP_001031935.1
- ProteinModelPortal:   A2RIU4
- SMR:   A2RIU4
- STRING:   A2RIU4
- GeneID:   4796811
- GenomeReviews:   AM406671_GR
- KEGG:   llm:llmg_0591
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 33505; Mature: 33505

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNNKVLVVVGPTAVGKTALGIDLAIKMNGEIISGDSQQVYQGLDIGTAKVTKAEQALAV
CCCCCEEEEECCCHHCCHHCCEEEEEEECCEEECCCHHHHHHCCCCCCHHHHHHHHHHHH
HHLIDVRKWTENFSVHDFVMEANRLIEEIIERGNVPIIVGGTGLYIQSLIEGYHLGGQEN
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHCCCCCCH
HQAMMELRETLLALTDEELFEKVLKLNPNFPELNRRRAIRFLELQTFGSTDENSGSDYNF
HHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHHHHHHEEEEECCCCCCCCCCCEEE
LLIGLNAERKVLYERINQRVEQMMSEGLLSEARSLFEKAPDAQAAKGIGYKEFFPYFSGE
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHCCCCCCC
ISLEDAVELVKRNSRRYAKRQLTWFRNRMEVEFEDVFSETYPDSVFEKVTQFLN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHC
>Mature Secondary Structure
MKNNKVLVVVGPTAVGKTALGIDLAIKMNGEIISGDSQQVYQGLDIGTAKVTKAEQALAV
CCCCCEEEEECCCHHCCHHCCEEEEEEECCEEECCCHHHHHHCCCCCCHHHHHHHHHHHH
HHLIDVRKWTENFSVHDFVMEANRLIEEIIERGNVPIIVGGTGLYIQSLIEGYHLGGQEN
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHCCCCCCH
HQAMMELRETLLALTDEELFEKVLKLNPNFPELNRRRAIRFLELQTFGSTDENSGSDYNF
HHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHHHHHHEEEEECCCCCCCCCCCEEE
LLIGLNAERKVLYERINQRVEQMMSEGLLSEARSLFEKAPDAQAAKGIGYKEFFPYFSGE
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHCCCCCCC
ISLEDAVELVKRNSRRYAKRQLTWFRNRMEVEFEDVFSETYPDSVFEKVTQFLN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA