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The map label for this gene is prfA [H]

Identifier: 125623422

GI number: 125623422

Start: 550758

End: 551831

Strand: Direct

Name: prfA [H]

Synonym: llmg_0557

Alternate gene names: 125623422

Gene position: 550758-551831 (Clockwise)

Preceding gene: 125623421

Following gene: 125623423

Centisome position: 21.77

GC content: 37.34

Gene sequence:

>1074_bases
ATGTTTGATCAGTTAGAGTCAATAGTTGGAAGATATGAAGAATTGGGTGAGCTATTATCTGACCCAGAAGTAGTTTCTGA
TACAAAGCGTTTTATGGAATTATCACGTGAAGAAGCAGATTTACGGGATAAAGTGGCGACTTATAATGAATACAAGAAAG
TCCTTGAAACAATTTCTGATAGCGAAGAAATGCTTGGTGAGGGTGGACTTGATGATGAAATGAAAGAAATGCTCAAAGAA
GAACTTTCATCAGCAAAATCACAAAAAGAAGTTCTTGAAGAAGAAATCAAAATTTTACTTTTGCCTAAAGATCCAAATGA
TGGTAAAAATATTATTTTAGAAATTCGTGGAGCTGCTGGTGGGGATGAAGCAGCGCTTTTTGCGGGTGATTTATTAAATA
TGTATCAACATTTTTCTGAATCTCAAGGTTGGAAGTTTGAAATTATGGAAGCGAATATCACTGGTATTGGCGGTTATAAA
GAGGTTTCAGCTTTGATTTCGGGTCCATCTGTTTATTCAAAATTGAAATATGAATCTGGTGCTCACCGAGTACAACGTGT
CCCTGTAACCGAAACACAAGGGCGCGTGCATACTTCGACAGCGACAGTGCTTGTGATGCCTGAAGTTGAAGAATTCGAAA
TGACAATTGAGCAAAAAGATTTGCGCGTGGATATTTATCATGCGTCTGGTGCCGGTGGTCAGAATGTCAATAAGGTTGCC
ACAGCCGTGCGGATGGTTCACTTGCCAACAGGGATTAAAGTTGAGATGCAAGAAGAACGGACACAACAAAAAAATCGCGA
TAAAGCCATTAAATTGTTGAATACGAAAGTTTTTGATTACTATCAACAAATTGAATTGGACAAACAAAATACAGAGCGTA
AATCAACTGTCGGAACAGGTGACCGTTCTGAGCGAATCAGAACTTATAATTTTCCACAAAACCGTGTAACTGACCACCGA
ATTGGTTTGACTTTACAAAAATTGGATAGTATTTTATCTGGCAAAATGGATGAAGTGATTGATGCTCTGATTGTCTATGA
CCAAACGAAGAAATTAGAAGAATTAAATAAATAA

Upstream 100 bases:

>100_bases
AAAAGGATATGCTACAAGATGTGACACAATGGGATTCTTTTGTAAATATGGCAAAAAATTTACCATTAAAAACTAAAAAA
TAATTAAAGGAAATAAAGAA

Downstream 100 bases:

>100_bases
TGAATTTTGAATTTGAAGATTTTGTCATTCGAGAAGTTGGTCATGAACAACGGACGATGCAAACCTCAAAAAAAGTGAAT
AATGTCTTAACAGATGTGAC

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MFDQLESIVGRYEELGELLSDPEVVSDTKRFMELSREEADLRDKVATYNEYKKVLETISDSEEMLGEGGLDDEMKEMLKE
ELSSAKSQKEVLEEEIKILLLPKDPNDGKNIILEIRGAAGGDEAALFAGDLLNMYQHFSESQGWKFEIMEANITGIGGYK
EVSALISGPSVYSKLKYESGAHRVQRVPVTETQGRVHTSTATVLVMPEVEEFEMTIEQKDLRVDIYHASGAGGQNVNKVA
TAVRMVHLPTGIKVEMQEERTQQKNRDKAIKLLNTKVFDYYQQIELDKQNTERKSTVGTGDRSERIRTYNFPQNRVTDHR
IGLTLQKLDSILSGKMDEVIDALIVYDQTKKLEELNK

Sequences:

>Translated_357_residues
MFDQLESIVGRYEELGELLSDPEVVSDTKRFMELSREEADLRDKVATYNEYKKVLETISDSEEMLGEGGLDDEMKEMLKE
ELSSAKSQKEVLEEEIKILLLPKDPNDGKNIILEIRGAAGGDEAALFAGDLLNMYQHFSESQGWKFEIMEANITGIGGYK
EVSALISGPSVYSKLKYESGAHRVQRVPVTETQGRVHTSTATVLVMPEVEEFEMTIEQKDLRVDIYHASGAGGQNVNKVA
TAVRMVHLPTGIKVEMQEERTQQKNRDKAIKLLNTKVFDYYQQIELDKQNTERKSTVGTGDRSERIRTYNFPQNRVTDHR
IGLTLQKLDSILSGKMDEVIDALIVYDQTKKLEELNK
>Mature_357_residues
MFDQLESIVGRYEELGELLSDPEVVSDTKRFMELSREEADLRDKVATYNEYKKVLETISDSEEMLGEGGLDDEMKEMLKE
ELSSAKSQKEVLEEEIKILLLPKDPNDGKNIILEIRGAAGGDEAALFAGDLLNMYQHFSESQGWKFEIMEANITGIGGYK
EVSALISGPSVYSKLKYESGAHRVQRVPVTETQGRVHTSTATVLVMPEVEEFEMTIEQKDLRVDIYHASGAGGQNVNKVA
TAVRMVHLPTGIKVEMQEERTQQKNRDKAIKLLNTKVFDYYQQIELDKQNTERKSTVGTGDRSERIRTYNFPQNRVTDHR
IGLTLQKLDSILSGKMDEVIDALIVYDQTKKLEELNK

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=287, Percent_Identity=42.5087108013937, Blast_Score=253, Evalue=1e-67,
Organism=Homo sapiens, GI34577120, Length=351, Percent_Identity=37.8917378917379, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI166795305, Length=183, Percent_Identity=42.6229508196721, Blast_Score=168, Evalue=6e-42,
Organism=Escherichia coli, GI1787462, Length=355, Percent_Identity=48.169014084507, Blast_Score=333, Evalue=1e-92,
Organism=Escherichia coli, GI2367172, Length=342, Percent_Identity=34.5029239766082, Blast_Score=188, Evalue=6e-49,
Organism=Caenorhabditis elegans, GI17542784, Length=354, Percent_Identity=33.8983050847458, Blast_Score=190, Evalue=8e-49,
Organism=Saccharomyces cerevisiae, GI6321295, Length=327, Percent_Identity=39.7553516819572, Blast_Score=230, Evalue=2e-61,
Organism=Drosophila melanogaster, GI19921226, Length=321, Percent_Identity=40.4984423676012, Blast_Score=221, Evalue=8e-58,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40449; Mature: 40449

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDQLESIVGRYEELGELLSDPEVVSDTKRFMELSREEADLRDKVATYNEYKKVLETISD
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SEEMLGEGGLDDEMKEMLKEELSSAKSQKEVLEEEIKILLLPKDPNDGKNIILEIRGAAG
CHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCEEEEEEECCCC
GDEAALFAGDLLNMYQHFSESQGWKFEIMEANITGIGGYKEVSALISGPSVYSKLKYESG
CCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHCCC
AHRVQRVPVTETQGRVHTSTATVLVMPEVEEFEMTIEQKDLRVDIYHASGAGGQNVNKVA
CHHHEECCCCCCCCEEEECCEEEEEECCHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHH
TAVRMVHLPTGIKVEMQEERTQQKNRDKAIKLLNTKVFDYYQQIELDKQNTERKSTVGTG
HHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCC
DRSERIRTYNFPQNRVTDHRIGLTLQKLDSILSGKMDEVIDALIVYDQTKKLEELNK
CCCCCCEEECCCCCCCCHHHHCEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFDQLESIVGRYEELGELLSDPEVVSDTKRFMELSREEADLRDKVATYNEYKKVLETISD
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SEEMLGEGGLDDEMKEMLKEELSSAKSQKEVLEEEIKILLLPKDPNDGKNIILEIRGAAG
CHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCEEEEEEECCCC
GDEAALFAGDLLNMYQHFSESQGWKFEIMEANITGIGGYKEVSALISGPSVYSKLKYESG
CCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHCCC
AHRVQRVPVTETQGRVHTSTATVLVMPEVEEFEMTIEQKDLRVDIYHASGAGGQNVNKVA
CHHHEECCCCCCCCEEEECCEEEEEECCHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHH
TAVRMVHLPTGIKVEMQEERTQQKNRDKAIKLLNTKVFDYYQQIELDKQNTERKSTVGTG
HHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCC
DRSERIRTYNFPQNRVTDHRIGLTLQKLDSILSGKMDEVIDALIVYDQTKKLEELNK
CCCCCCEEECCCCCCCCHHHHCEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]