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The map label for this gene is dnaG [H]

Identifier: 125623387

GI number: 125623387

Start: 517878

End: 519791

Strand: Direct

Name: dnaG [H]

Synonym: llmg_0520

Alternate gene names: 125623387

Gene position: 517878-519791 (Clockwise)

Preceding gene: 125623386

Following gene: 125623388

Centisome position: 20.47

GC content: 34.9

Gene sequence:

>1914_bases
ATGGTTTCAGTAGATACTGAAGTTGTGAATGACTTAAAGTCAAAGGTTAATATTGCTGATTTGATTTCACAATATGTTGC
CTTAAGTCGCACCGGTAAAAATTATATTGGCTTATGCCCTTTTCATGGCGAAAAAACTCCTTCTTTTAATGTGAATGCAG
AAAAAGGATTTTATCATTGTTTTGGTTGTGGTAGGTCTGGAGATGCTATTGAGTTCTTAAAAGAATATAATCAAGTGGGC
TTTATTGATGCTGTTAAAGAGTTGGCTAATTTCGCCGGGGTCACTCTTGATATTTCTAATGACAGAGAAGAAAAAAACAA
TCCTAATGCGCCACTCTATGAAATCAATAATCAGGCAGCTCGTTTATATAATATTTTGTTGATGTCAACAGAATTAGGGG
AACGAGCCAGAGACTACCTAGCTGAACGTGGGATTACTGATGATGTAATCAAAAGATTCAATATTGGTTTGGCTCCTGAG
GAAAATGATTTTATTTTTAAAAACTTATCAAATAAATTTGATGAAGAAGTTATGGCTAACTCAGGCTTATTTCATTTTTC
AAATAATAGGGTTTTTGATGCTTTTACGAATCGAATCATGTTTCCAATTACCAATGAATACGGTCAAACGATTGGTTTTT
CTGGACGAAAATGGCAAGAGAATGATGATTCAAAAGCAAAATATATCAATACATCAGCAACGACAATTTTTGATAAATCT
TATGAATTGTGGAATTTAGATAAAGCAAAACCAACTATTTCTAAGCGGCGTGAAGTTTATTTAATGGAAGGTTTCATGGA
TGTTATTGCGGCTCATAAAGCGGGAATAAATAATGTTGTTGCCTCAATGGGAACGGCTTTGACGGAGAAGCATGTTCGCC
GCTTGAAACAAATTGCTAAAAAATTCGTATTAGTATATGATGGAGATTCTGCCGGACAAAACGCCATCTATAAGGCCCTT
AATTTGGTTGGTGAATCTGATGTACAGATTGTCAAGGTGCCTGAAGGGTTAGACCCGGATGAGTATTCTAAAACATATGG
TCTAACAGGTCTATCAGCGTTGATGGAAACTGGGCGGATTCAGCCCATTGAGTTCTTGATTGATTTTCTAAGACCTGAAA
ATTTGGCTAATTTGCAGGTTCAGTTAGATTTTATTGAGCAGATTGCGCCAATGATTGCAAAGCTACAATCGATAACGGCT
CAGGATGCTTATATAAGAAAATTAGTTGAGATTTTGCCTGATTTCGAGTATAATCAAGTGGAGCAGGCTGTAAATTTGCG
CCGTGAAAATATGAATATTACTGACCATTCTGTCAGTGATTATGGGGCTTCAAATCTAGTTGAAAATTTTGCTGACGAGA
GAGATTATTCTAATCTTGATTCGGCCATACCTATTGATTTTGAAGAAATCTTTTATGAAAATAATATTCAACCTCAAGTA
GCTGATAGAAGAAATGAACCAGCTCAAGTTTTTCAACCAACGATTCAGGTTCCTAAATTGTCTCGTTCAGAAAGAGCTGA
GGAGATGCTCCTTCATCGAATGATTTATCATTCATCTGTTTTGAAAAAATTTTCTCAGGATGAAAATTTTAGATTTGTCC
ATAAACGTTATCAGGATATATTTGAAAAGCTATTACTTGAGGCAATGGTTTATGAGCAGATTGATGAATCCCATTTGGCA
AGTGAGTTGTCAGAGGATCAGCGTAGTCTCTTTTATCAGATTATAAGTTTAGATTTACCAGAGACTGCATCAAGTCAGGA
AATTAATGACTTGGTTTCCATTTTTTCAAATGAAATGGAACAAATAAAATTTGAAGAACTTCAGCAACAATTAGCAACTG
CAGAAAAAGCAGGAAATAAAGAACGTATGTTAGAGTTAACCCTTCAGATTATTAATCAAAAGAAAAAATTATAA

Upstream 100 bases:

>100_bases
TTGTGAATGAATAATAAACCAGACTCTTTCCTAAGTGTTATACTTAAGTTGTGATTACTTATTTGAATGTTCATAGTCAA
AATGAAAGGGGGAGTAGCGA

Downstream 100 bases:

>100_bases
TATTTTTTTACTGACAATTGGCTAGTCTATTTAATGACAGTTTTGTCAGTAGTTTTACCCAAAGAGGAGGATCGAATTGG
CAGCAAATAAAAAAAGAATA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 637; Mature: 637

Protein sequence:

>637_residues
MVSVDTEVVNDLKSKVNIADLISQYVALSRTGKNYIGLCPFHGEKTPSFNVNAEKGFYHCFGCGRSGDAIEFLKEYNQVG
FIDAVKELANFAGVTLDISNDREEKNNPNAPLYEINNQAARLYNILLMSTELGERARDYLAERGITDDVIKRFNIGLAPE
ENDFIFKNLSNKFDEEVMANSGLFHFSNNRVFDAFTNRIMFPITNEYGQTIGFSGRKWQENDDSKAKYINTSATTIFDKS
YELWNLDKAKPTISKRREVYLMEGFMDVIAAHKAGINNVVASMGTALTEKHVRRLKQIAKKFVLVYDGDSAGQNAIYKAL
NLVGESDVQIVKVPEGLDPDEYSKTYGLTGLSALMETGRIQPIEFLIDFLRPENLANLQVQLDFIEQIAPMIAKLQSITA
QDAYIRKLVEILPDFEYNQVEQAVNLRRENMNITDHSVSDYGASNLVENFADERDYSNLDSAIPIDFEEIFYENNIQPQV
ADRRNEPAQVFQPTIQVPKLSRSERAEEMLLHRMIYHSSVLKKFSQDENFRFVHKRYQDIFEKLLLEAMVYEQIDESHLA
SELSEDQRSLFYQIISLDLPETASSQEINDLVSIFSNEMEQIKFEELQQQLATAEKAGNKERMLELTLQIINQKKKL

Sequences:

>Translated_637_residues
MVSVDTEVVNDLKSKVNIADLISQYVALSRTGKNYIGLCPFHGEKTPSFNVNAEKGFYHCFGCGRSGDAIEFLKEYNQVG
FIDAVKELANFAGVTLDISNDREEKNNPNAPLYEINNQAARLYNILLMSTELGERARDYLAERGITDDVIKRFNIGLAPE
ENDFIFKNLSNKFDEEVMANSGLFHFSNNRVFDAFTNRIMFPITNEYGQTIGFSGRKWQENDDSKAKYINTSATTIFDKS
YELWNLDKAKPTISKRREVYLMEGFMDVIAAHKAGINNVVASMGTALTEKHVRRLKQIAKKFVLVYDGDSAGQNAIYKAL
NLVGESDVQIVKVPEGLDPDEYSKTYGLTGLSALMETGRIQPIEFLIDFLRPENLANLQVQLDFIEQIAPMIAKLQSITA
QDAYIRKLVEILPDFEYNQVEQAVNLRRENMNITDHSVSDYGASNLVENFADERDYSNLDSAIPIDFEEIFYENNIQPQV
ADRRNEPAQVFQPTIQVPKLSRSERAEEMLLHRMIYHSSVLKKFSQDENFRFVHKRYQDIFEKLLLEAMVYEQIDESHLA
SELSEDQRSLFYQIISLDLPETASSQEINDLVSIFSNEMEQIKFEELQQQLATAEKAGNKERMLELTLQIINQKKKL
>Mature_637_residues
MVSVDTEVVNDLKSKVNIADLISQYVALSRTGKNYIGLCPFHGEKTPSFNVNAEKGFYHCFGCGRSGDAIEFLKEYNQVG
FIDAVKELANFAGVTLDISNDREEKNNPNAPLYEINNQAARLYNILLMSTELGERARDYLAERGITDDVIKRFNIGLAPE
ENDFIFKNLSNKFDEEVMANSGLFHFSNNRVFDAFTNRIMFPITNEYGQTIGFSGRKWQENDDSKAKYINTSATTIFDKS
YELWNLDKAKPTISKRREVYLMEGFMDVIAAHKAGINNVVASMGTALTEKHVRRLKQIAKKFVLVYDGDSAGQNAIYKAL
NLVGESDVQIVKVPEGLDPDEYSKTYGLTGLSALMETGRIQPIEFLIDFLRPENLANLQVQLDFIEQIAPMIAKLQSITA
QDAYIRKLVEILPDFEYNQVEQAVNLRRENMNITDHSVSDYGASNLVENFADERDYSNLDSAIPIDFEEIFYENNIQPQV
ADRRNEPAQVFQPTIQVPKLSRSERAEEMLLHRMIYHSSVLKKFSQDENFRFVHKRYQDIFEKLLLEAMVYEQIDESHLA
SELSEDQRSLFYQIISLDLPETASSQEINDLVSIFSNEMEQIKFEELQQQLATAEKAGNKERMLELTLQIINQKKKL

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=376, Percent_Identity=35.1063829787234, Blast_Score=233, Evalue=3e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 72847; Mature: 72847

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSVDTEVVNDLKSKVNIADLISQYVALSRTGKNYIGLCPFHGEKTPSFNVNAEKGFYHC
CCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCEEE
FGCGRSGDAIEFLKEYNQVGFIDAVKELANFAGVTLDISNDREEKNNPNAPLYEINNQAA
EECCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCEEECCHHHH
RLYNILLMSTELGERARDYLAERGITDDVIKRFNIGLAPEENDFIFKNLSNKFDEEVMAN
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHC
SGLFHFSNNRVFDAFTNRIMFPITNEYGQTIGFSGRKWQENDDSKAKYINTSATTIFDKS
CCCEEECCCEEEHHHHCCEEEEEECHHCCCCCCCCCCCCCCCCCCCEEECCCHHEEECCC
YELWNLDKAKPTISKRREVYLMEGFMDVIAAHKAGINNVVASMGTALTEKHVRRLKQIAK
CCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
KFVLVYDGDSAGQNAIYKALNLVGESDVQIVKVPEGLDPDEYSKTYGLTGLSALMETGRI
HEEEEECCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHCHHHHHHHHHCCCC
QPIEFLIDFLRPENLANLQVQLDFIEQIAPMIAKLQSITAQDAYIRKLVEILPDFEYNQV
CHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
EQAVNLRRENMNITDHSVSDYGASNLVENFADERDYSNLDSAIPIDFEEIFYENNIQPQV
HHHHHHHHHCCCCCCCCCHHCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCH
ADRRNEPAQVFQPTIQVPKLSRSERAEEMLLHRMIYHSSVLKKFSQDENFRFVHKRYQDI
HHCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
FEKLLLEAMVYEQIDESHLASELSEDQRSLFYQIISLDLPETASSQEINDLVSIFSNEME
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
QIKFEELQQQLATAEKAGNKERMLELTLQIINQKKKL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVSVDTEVVNDLKSKVNIADLISQYVALSRTGKNYIGLCPFHGEKTPSFNVNAEKGFYHC
CCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCEEE
FGCGRSGDAIEFLKEYNQVGFIDAVKELANFAGVTLDISNDREEKNNPNAPLYEINNQAA
EECCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCEEECCHHHH
RLYNILLMSTELGERARDYLAERGITDDVIKRFNIGLAPEENDFIFKNLSNKFDEEVMAN
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHC
SGLFHFSNNRVFDAFTNRIMFPITNEYGQTIGFSGRKWQENDDSKAKYINTSATTIFDKS
CCCEEECCCEEEHHHHCCEEEEEECHHCCCCCCCCCCCCCCCCCCCEEECCCHHEEECCC
YELWNLDKAKPTISKRREVYLMEGFMDVIAAHKAGINNVVASMGTALTEKHVRRLKQIAK
CCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
KFVLVYDGDSAGQNAIYKALNLVGESDVQIVKVPEGLDPDEYSKTYGLTGLSALMETGRI
HEEEEECCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHCHHHHHHHHHCCCC
QPIEFLIDFLRPENLANLQVQLDFIEQIAPMIAKLQSITAQDAYIRKLVEILPDFEYNQV
CHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
EQAVNLRRENMNITDHSVSDYGASNLVENFADERDYSNLDSAIPIDFEEIFYENNIQPQV
HHHHHHHHHCCCCCCCCCHHCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCH
ADRRNEPAQVFQPTIQVPKLSRSERAEEMLLHRMIYHSSVLKKFSQDENFRFVHKRYQDI
HHCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
FEKLLLEAMVYEQIDESHLASELSEDQRSLFYQIISLDLPETASSQEINDLVSIFSNEME
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
QIKFEELQQQLATAEKAGNKERMLELTLQIINQKKKL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471; 7765979; 7503808 [H]