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The map label for this gene is tig [H]

Identifier: 125623386

GI number: 125623386

Start: 509736

End: 511019

Strand: Direct

Name: tig [H]

Synonym: llmg_0519

Alternate gene names: 125623386

Gene position: 509736-511019 (Clockwise)

Preceding gene: 125623385

Following gene: 125623387

Centisome position: 20.15

GC content: 36.99

Gene sequence:

>1284_bases
ATGACTGTAAGTTTTGAAAAAACTAGTGATACTAAGGGTACACTCTCATTTTCAATTGATCAAGAAACAATTAAAACTGG
GCTTGACAAAGCCTTTAATAAAGTAAAAGCTAACATTAGCGTTCCTGGTTTCCGTAAAGGTAAAATCAGCCGTCAAATGT
TCAATAAAATGTACGGTGAAGAAGCTCTTTTTGAAGAAGCTTTGAATGCTGTTCTTCCAACTGCTTACGATGCTGCAGTA
AAAGAAGCTGCTATCGAACCAGTTGCTCAACCAAAAATTGACGTAGCAAAAATGGAAAAAGGTTCTGATTGGGAATTGAC
AGCAGAAGTTGTTGTTAAACCAACAATCACACTTGGTGACTACAAAGACCTTACAGTTGAAGTAGACGCAACTAAAGAAG
TTACTGATGAAGAAGTGGAAACACGTTTAACAAACGCTCAAAACAACTTGGCAGAACTTGCAGTAAAAGAAACTGCTGCC
GAAAATGGTGATACTGTTGTTATTGACTTTGTTGGTTCAGTTGACGGTGTTGAATTTGAAGGTGGAAAAGGTTCAAACCA
CAGTCTTGAACTTGGTTCAGGCCAATTCATTCCAGGATTTGAAGAACAACTTATTGGTACAAAAGCTGGTGAAACTGTAG
AAGTTAAAGTAACCTTCCCAGAAAATTATCAAGCTGAAGACCTTGCAGGAAAAGAAGCTCTTTTTGTAACAACTGTCAAT
GAAGTAAAAGCAAAAGAATTGCCAGAACTTGATGATGAACTTGCAAAAGACATCGACGAAGAAGTTGAAACTTTAGAAGA
ACTTAAAGCTAAATTCCGTAAAGAACTTGAAGAATCAAAATCAGAAGCTTACGATGATGCAGTAGAAACTGCCGCAATTG
AAGCTGCTGTTGCTAACGCTGAAATCAAAGAAATCCCAGAAGAAATGATTCATGAAGAAGTTCATCGTGCGATGAATGAA
TTCCTTGGAGGAATGCAACAACAAGGAATCTCTCCAGAAATGTACTTCCAAATTACAGGTACTTCTGAAGACGATCTCCA
TAAACAATATGAAGCAGATGCTGACAAACGCGTTCGTACTAACTTAGTAATCGAAGCAATTGCAGCTGCTGAAAGCTTTA
CAACTTCAGATGAAGAAGTAAAAGCTGAAATTGAAGATCTTGCTGGTCAATATAACATGCCTGTTGAACAAGTTGAAAAA
CTTCTTCCAGTTGACATGCTTAAACACGATATTGCTATGAAAAAAGCAGTAGAAGTTATTGCTACAACAGCTAAAGTAAA
ATAA

Upstream 100 bases:

>100_bases
ATGAGCTGAAAAAGCAAGTAATGGTTGAAATATAGGGTATTTTATGCTATTCTTGAAAGGTATGAATTTCTGAGCTTAGG
CTCAAAATAAGGAGAAAAAA

Downstream 100 bases:

>100_bases
TTTTTATAATTAATGAGAATAAAGTACTGCCATTTGCAGTACTTATTTTTTTGCCATTTTAAAAGTTTCAGATAAGATAT
TTTTCATAAAATTATCAGAT

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MTVSFEKTSDTKGTLSFSIDQETIKTGLDKAFNKVKANISVPGFRKGKISRQMFNKMYGEEALFEEALNAVLPTAYDAAV
KEAAIEPVAQPKIDVAKMEKGSDWELTAEVVVKPTITLGDYKDLTVEVDATKEVTDEEVETRLTNAQNNLAELAVKETAA
ENGDTVVIDFVGSVDGVEFEGGKGSNHSLELGSGQFIPGFEEQLIGTKAGETVEVKVTFPENYQAEDLAGKEALFVTTVN
EVKAKELPELDDELAKDIDEEVETLEELKAKFRKELEESKSEAYDDAVETAAIEAAVANAEIKEIPEEMIHEEVHRAMNE
FLGGMQQQGISPEMYFQITGTSEDDLHKQYEADADKRVRTNLVIEAIAAAESFTTSDEEVKAEIEDLAGQYNMPVEQVEK
LLPVDMLKHDIAMKKAVEVIATTAKVK

Sequences:

>Translated_427_residues
MTVSFEKTSDTKGTLSFSIDQETIKTGLDKAFNKVKANISVPGFRKGKISRQMFNKMYGEEALFEEALNAVLPTAYDAAV
KEAAIEPVAQPKIDVAKMEKGSDWELTAEVVVKPTITLGDYKDLTVEVDATKEVTDEEVETRLTNAQNNLAELAVKETAA
ENGDTVVIDFVGSVDGVEFEGGKGSNHSLELGSGQFIPGFEEQLIGTKAGETVEVKVTFPENYQAEDLAGKEALFVTTVN
EVKAKELPELDDELAKDIDEEVETLEELKAKFRKELEESKSEAYDDAVETAAIEAAVANAEIKEIPEEMIHEEVHRAMNE
FLGGMQQQGISPEMYFQITGTSEDDLHKQYEADADKRVRTNLVIEAIAAAESFTTSDEEVKAEIEDLAGQYNMPVEQVEK
LLPVDMLKHDIAMKKAVEVIATTAKVK
>Mature_426_residues
TVSFEKTSDTKGTLSFSIDQETIKTGLDKAFNKVKANISVPGFRKGKISRQMFNKMYGEEALFEEALNAVLPTAYDAAVK
EAAIEPVAQPKIDVAKMEKGSDWELTAEVVVKPTITLGDYKDLTVEVDATKEVTDEEVETRLTNAQNNLAELAVKETAAE
NGDTVVIDFVGSVDGVEFEGGKGSNHSLELGSGQFIPGFEEQLIGTKAGETVEVKVTFPENYQAEDLAGKEALFVTTVNE
VKAKELPELDDELAKDIDEEVETLEELKAKFRKELEESKSEAYDDAVETAAIEAAVANAEIKEIPEEMIHEEVHRAMNEF
LGGMQQQGISPEMYFQITGTSEDDLHKQYEADADKRVRTNLVIEAIAAAESFTTSDEEVKAEIEDLAGQYNMPVEQVEKL
LPVDMLKHDIAMKKAVEVIATTAKVK

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation [H]

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain [H]

Homologues:

Organism=Escherichia coli, GI1786640, Length=436, Percent_Identity=32.1100917431193, Blast_Score=179, Evalue=2e-46,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881 [H]

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N [H]

EC number: NA

Molecular weight: Translated: 46947; Mature: 46816

Theoretical pI: Translated: 4.13; Mature: 4.13

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVSFEKTSDTKGTLSFSIDQETIKTGLDKAFNKVKANISVPGFRKGKISRQMFNKMYGE
CEECCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCH
EALFEEALNAVLPTAYDAAVKEAAIEPVAQPKIDVAKMEKGSDWELTAEVVVKPTITLGD
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCEEEEEEEEEEEEEECC
YKDLTVEVDATKEVTDEEVETRLTNAQNNLAELAVKETAAENGDTVVIDFVGSVDGVEFE
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEC
GGKGSNHSLELGSGQFIPGFEEQLIGTKAGETVEVKVTFPENYQAEDLAGKEALFVTTVN
CCCCCCCEEECCCCCCCCCHHHHHCCCCCCCEEEEEEECCCCCCHHHCCCCCEEEEEEHH
EVKAKELPELDDELAKDIDEEVETLEELKAKFRKELEESKSEAYDDAVETAAIEAAVANA
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EIKEIPEEMIHEEVHRAMNEFLGGMQQQGISPEMYFQITGTSEDDLHKQYEADADKRVRT
HHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCEEEEEEECCCCHHHHHHHHCCHHHHHHH
NLVIEAIAAAESFTTSDEEVKAEIEDLAGQYNMPVEQVEKLLPVDMLKHDIAMKKAVEVI
HHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHH
ATTAKVK
HHHHCCC
>Mature Secondary Structure 
TVSFEKTSDTKGTLSFSIDQETIKTGLDKAFNKVKANISVPGFRKGKISRQMFNKMYGE
EECCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCH
EALFEEALNAVLPTAYDAAVKEAAIEPVAQPKIDVAKMEKGSDWELTAEVVVKPTITLGD
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCEEEEEEEEEEEEEECC
YKDLTVEVDATKEVTDEEVETRLTNAQNNLAELAVKETAAENGDTVVIDFVGSVDGVEFE
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEC
GGKGSNHSLELGSGQFIPGFEEQLIGTKAGETVEVKVTFPENYQAEDLAGKEALFVTTVN
CCCCCCCEEECCCCCCCCCHHHHHCCCCCCCEEEEEEECCCCCCHHHCCCCCEEEEEEHH
EVKAKELPELDDELAKDIDEEVETLEELKAKFRKELEESKSEAYDDAVETAAIEAAVANA
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EIKEIPEEMIHEEVHRAMNEFLGGMQQQGISPEMYFQITGTSEDDLHKQYEADADKRVRT
HHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCEEEEEEECCCCHHHHHHHHCCHHHHHHH
NLVIEAIAAAESFTTSDEEVKAEIEDLAGQYNMPVEQVEKLLPVDMLKHDIAMKKAVEVI
HHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHH
ATTAKVK
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]