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The map label for this gene is acmD [C]

Identifier: 125623376

GI number: 125623376

Start: 501132

End: 502217

Strand: Direct

Name: acmD [C]

Synonym: llmg_0509

Alternate gene names: 125623376

Gene position: 501132-502217 (Clockwise)

Preceding gene: 125623373

Following gene: 125623380

Centisome position: 19.81

GC content: 39.23

Gene sequence:

>1086_bases
ATGAAACAGAAACATAAATTAGCGCTTGGAGCGTCAATTGTTGCTTTGGCAAGTCTAGGTGGAATCAAAGCTCAAGCAGC
ATCAGTTCAAGAAATCATCAATGCTGCAGTACCAGTAGCCAATGACTATGGGCTTTATCCATCAGTAATGATTGCCCAAG
GAATTTTGGAATCTAGTGGTGGTCAAAGTGCTTTAGCAAGTAATTATAATAATATTTTTGGAGTGAAATACACTTCTGGA
ACACCAGTTTATTTATCAACGCAGGAATACTTAAATGGCGTAATGACAAATGTTGTTGAACCATTTCAATCTTATAGTTC
AGTTTATGATGCTTGCGTTGCCCAAGCTCAAATGTTGCGTGGTTCTTCATATTATTCAGGTGCTTGGCGTGAAAATACTA
GCTCTTACTTAGATGCAACTGCTTGGCTTGAAGGACGTTATGCTACTGACCCAACTTATGCTTCTAAATTGAACAGTGTG
ATTTCAGAACTTGGATTGAGTATTTATGACCAAGGGGGAGAGGTTTCTAGTGCGAGCGCTGTTACAACAAGCTCAGCAGC
CACACCAAATTCAGTCGGAACTTATAAAGTACAAGAAGGGGACTCGTTGTCAGCAATCGCTGCACAATATGGAACAACAG
TAGAGGCACTTGTATCAGCAAATAGTTTGGAGAGTGCTAACGATATTCATGTTGGGGAAGTCGTACAAGTTGCTGGAGCA
AGTAGCACGACAACAAACACAAGTAATACCGCTTCAAGCACATCAGCTAATACATATACTATCAAATCAGGGGATAGCCT
TTATTCAATTGCTGAGCAATATGGAATGAAAGTTTCATCATTGATGTCAGCTAATGGAATTTATGATGTTAACACAATGC
TCCAAGTAGGACAAGTTTTACAAGTTAGTACAAACAGTTCAACAAATACAAGCACATCAGCATCATCTGGTAGTTACACA
ATTCAAAATGGTGACAGTATCTATTCTATTGCAACAGCCAATGGAATGACAGCTGACCAACTGGCCGCTCTCAATGGTTT
TGGAATTAACGATATGATTCATCCAGGCCAAACCATTAAAATTTAA

Upstream 100 bases:

>100_bases
CTTTACAAATCGTCAGAAAATTTTTATACTAGTAAGTAACAATAAAAATACAATTGGCATATTTCAGTCAAAAAACTATT
TAATTAAGAGGGGAAACTCG

Downstream 100 bases:

>100_bases
ATAAAAAAGCACTGACAGAACTGTCAGTGCTTTTTTTATTGGAAAAATGGATTATGAAGTTTTTCAGCGCCAATTGTCGT
CTCTGGTCCATGACCAGGCA

Product: N-acetylglucosaminidase

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Beta-glycosidase; Peptidoglycan hydrolase [H]

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MKQKHKLALGASIVALASLGGIKAQAASVQEIINAAVPVANDYGLYPSVMIAQGILESSGGQSALASNYNNIFGVKYTSG
TPVYLSTQEYLNGVMTNVVEPFQSYSSVYDACVAQAQMLRGSSYYSGAWRENTSSYLDATAWLEGRYATDPTYASKLNSV
ISELGLSIYDQGGEVSSASAVTTSSAATPNSVGTYKVQEGDSLSAIAAQYGTTVEALVSANSLESANDIHVGEVVQVAGA
SSTTTNTSNTASSTSANTYTIKSGDSLYSIAEQYGMKVSSLMSANGIYDVNTMLQVGQVLQVSTNSSTNTSTSASSGSYT
IQNGDSIYSIATANGMTADQLAALNGFGINDMIHPGQTIKI

Sequences:

>Translated_361_residues
MKQKHKLALGASIVALASLGGIKAQAASVQEIINAAVPVANDYGLYPSVMIAQGILESSGGQSALASNYNNIFGVKYTSG
TPVYLSTQEYLNGVMTNVVEPFQSYSSVYDACVAQAQMLRGSSYYSGAWRENTSSYLDATAWLEGRYATDPTYASKLNSV
ISELGLSIYDQGGEVSSASAVTTSSAATPNSVGTYKVQEGDSLSAIAAQYGTTVEALVSANSLESANDIHVGEVVQVAGA
SSTTTNTSNTASSTSANTYTIKSGDSLYSIAEQYGMKVSSLMSANGIYDVNTMLQVGQVLQVSTNSSTNTSTSASSGSYT
IQNGDSIYSIATANGMTADQLAALNGFGINDMIHPGQTIKI
>Mature_361_residues
MKQKHKLALGASIVALASLGGIKAQAASVQEIINAAVPVANDYGLYPSVMIAQGILESSGGQSALASNYNNIFGVKYTSG
TPVYLSTQEYLNGVMTNVVEPFQSYSSVYDACVAQAQMLRGSSYYSGAWRENTSSYLDATAWLEGRYATDPTYASKLNSV
ISELGLSIYDQGGEVSSASAVTTSSAATPNSVGTYKVQEGDSLSAIAAQYGTTVEALVSANSLESANDIHVGEVVQVAGA
SSTTTNTSNTASSTSANTYTIKSGDSLYSIAEQYGMKVSSLMSANGIYDVNTMLQVGQVLQVSTNSSTNTSTSASSGSYT
IQNGDSIYSIATANGMTADQLAALNGFGINDMIHPGQTIKI

Specific function: Hydrolyzes the cell wall of E.faecalis and M.lysodeikticus. May play an important role in cell wall growth and cell separation [H]

COG id: COG1705

COG function: function code NU; Muramidase (flagellum-specific)

Gene ontology:

Cell location: Secreted (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 6 LysM repeats [H]

Homologues:

None

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013338
- InterPro:   IPR002901
- InterPro:   IPR018392
- InterPro:   IPR002482 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF01476 LysM [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 37410; Mature: 37410

Theoretical pI: Translated: 4.27; Mature: 4.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQKHKLALGASIVALASLGGIKAQAASVQEIINAAVPVANDYGLYPSVMIAQGILESSG
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCC
GQSALASNYNNIFGVKYTSGTPVYLSTQEYLNGVMTNVVEPFQSYSSVYDACVAQAQMLR
CCHHHHCCCCCEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GSSYYSGAWRENTSSYLDATAWLEGRYATDPTYASKLNSVISELGLSIYDQGGEVSSASA
CCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCEEECCCCCCCCCCE
VTTSSAATPNSVGTYKVQEGDSLSAIAAQYGTTVEALVSANSLESANDIHVGEVVQVAGA
EECCCCCCCCCCCEEEECCCCCHHHHHHHHCCHHHHHHHHCCCCCCCCCCHHHHHHCCCC
SSTTTNTSNTASSTSANTYTIKSGDSLYSIAEQYGMKVSSLMSANGIYDVNTMLQVGQVL
CCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHCCEE
QVSTNSSTNTSTSASSGSYTIQNGDSIYSIATANGMTADQLAALNGFGINDMIHPGQTIK
EEECCCCCCCCCCCCCCCEEEECCCCEEEEEECCCCCHHHHHHHCCCCCCCCCCCCCEEC
I
C
>Mature Secondary Structure
MKQKHKLALGASIVALASLGGIKAQAASVQEIINAAVPVANDYGLYPSVMIAQGILESSG
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCC
GQSALASNYNNIFGVKYTSGTPVYLSTQEYLNGVMTNVVEPFQSYSSVYDACVAQAQMLR
CCHHHHCCCCCEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GSSYYSGAWRENTSSYLDATAWLEGRYATDPTYASKLNSVISELGLSIYDQGGEVSSASA
CCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCEEECCCCCCCCCCE
VTTSSAATPNSVGTYKVQEGDSLSAIAAQYGTTVEALVSANSLESANDIHVGEVVQVAGA
EECCCCCCCCCCCEEEECCCCCHHHHHHHHCCHHHHHHHHCCCCCCCCCCHHHHHHCCCC
SSTTTNTSNTASSTSANTYTIKSGDSLYSIAEQYGMKVSSLMSANGIYDVNTMLQVGQVL
CCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHCCEE
QVSTNSSTNTSTSASSGSYTIQNGDSIYSIATANGMTADQLAALNGFGINDMIHPGQTIK
EEECCCCCCCCCCCCCCCEEEECCCCEEEEEECCCCCHHHHHHHCCCCCCCCCCCCCEEC
I
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1679432; 12663927 [H]