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The map label for this gene is nifZ [C]

Identifier: 125623372

GI number: 125623372

Start: 495909

End: 497054

Strand: Direct

Name: nifZ [C]

Synonym: llmg_0505

Alternate gene names: 125623372

Gene position: 495909-497054 (Clockwise)

Preceding gene: 125623371

Following gene: 125623373

Centisome position: 19.61

GC content: 38.48

Gene sequence:

>1146_bases
ATGATATATTTTGATAATTCTGCTACGACGGCAGTTAATCCAATGGTTTTACGAACTTATACAGATGTAGCAACAAAAAT
TATGGGAAACCCTTCAAGTTTACATGGCTTGGGAACGACAGCCACACGTTTGCTTGAGGCGTCCAGACGACAAATTGCTG
AATTACTCGGTCTTGATTCACAAGAAATATTTTTTACAAGTGGGGGTACAGAGGGCGATAATTGGATTATTAAAGGTGTG
GCTTTTGAAAAAAGACCTTATGGTAATCATATTATTGTTTCCAGCGTTGAACATCCTGCAGTAAAAAATGCGGCAGAATG
GCTCAAGACTCAAGGATTTGAACTTGATTATGCGCCTGTTGATGCGGATGGTTTTGTTATTGTTTCCGAACTTGAAAAAT
TGATTAGAGATGAAACGATTTTAATATCAGTGATGGCAGTCAATAATGAAGTGGGAGCTATTCAACCGATTCAGGAGATT
TCTGAACTATTAGCCGATAAGCCAACCATTTCTTTTCATGTGGATGCGGTGCAAGCGATTGGGAAAGTCCCTTTAGAAAC
TTGGCTGACGGACCGACTTGATTTTGCAACTTTATCGGCTCATAAATTTCATGGTCCTCGAGGAGTGGGGATTGTGGTTG
CCAAAAAAGGGAAAAGATTAACTCCTCTTTTACATGGTGGTGGTCAAGAGCATAATTGGCGCTCAACCACAGAAAACTTG
GCTGGAATTGCTGCGACTTCTAAGGCGCTTCGATTAGCTCTGGATGAAGATGACTTCAAAAGAAAAAAGGTGTTAGCAAT
GAAACAAGTTATTTTAGAAGAACTTGAAAAACATAAAAAAGTTCATGTTTTTAGTAAAATGACTGATTTCGCACCAAATA
TTTTAACTTTTGGTATTCGAGGGGGTCGTGGAGAAGTTGTTGTTCATGCTTTTGAACAGCATGATATCTATATTTCAACC
ACTTCGGCTTGTTCTTCAAAGAAAAATGCTGCCGCAGGAACCTTAGTTGCAATGAATGTTCCTTCAAAATTGGCAACAAG
CGCAGTTAGAATCTCATTGGATTCTACAAATAATATGGCAGAAGTTGAACAATTTTTGACTGTTTTCCGTCAAATTTATC
AAGAGTTGGAAAAAGTAAGTGGCTAA

Upstream 100 bases:

>100_bases
ATAATAGATGCTCTTGATATTTACTCCTTATATTATTTTTCAACGCTCAGACATTTTTTGTGATAAAATAAGATTGATTT
TTAAATATTAAGGATAAAAA

Downstream 100 bases:

>100_bases
ACCTTGGTGAGAAAAATTGGCTAGATTGTTTGTGGCTCTTTTAGAAATGAAAAAAGGTGATTTGAAATTAACTTCAAATC
ACCTTTTCTTATCTAAATCT

Product: pyridoxal-phosphate dependent aminotransferase

Products: L-alanine; S [C]

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MIYFDNSATTAVNPMVLRTYTDVATKIMGNPSSLHGLGTTATRLLEASRRQIAELLGLDSQEIFFTSGGTEGDNWIIKGV
AFEKRPYGNHIIVSSVEHPAVKNAAEWLKTQGFELDYAPVDADGFVIVSELEKLIRDETILISVMAVNNEVGAIQPIQEI
SELLADKPTISFHVDAVQAIGKVPLETWLTDRLDFATLSAHKFHGPRGVGIVVAKKGKRLTPLLHGGGQEHNWRSTTENL
AGIAATSKALRLALDEDDFKRKKVLAMKQVILEELEKHKKVHVFSKMTDFAPNILTFGIRGGRGEVVVHAFEQHDIYIST
TSACSSKKNAAAGTLVAMNVPSKLATSAVRISLDSTNNMAEVEQFLTVFRQIYQELEKVSG

Sequences:

>Translated_381_residues
MIYFDNSATTAVNPMVLRTYTDVATKIMGNPSSLHGLGTTATRLLEASRRQIAELLGLDSQEIFFTSGGTEGDNWIIKGV
AFEKRPYGNHIIVSSVEHPAVKNAAEWLKTQGFELDYAPVDADGFVIVSELEKLIRDETILISVMAVNNEVGAIQPIQEI
SELLADKPTISFHVDAVQAIGKVPLETWLTDRLDFATLSAHKFHGPRGVGIVVAKKGKRLTPLLHGGGQEHNWRSTTENL
AGIAATSKALRLALDEDDFKRKKVLAMKQVILEELEKHKKVHVFSKMTDFAPNILTFGIRGGRGEVVVHAFEQHDIYIST
TSACSSKKNAAAGTLVAMNVPSKLATSAVRISLDSTNNMAEVEQFLTVFRQIYQELEKVSG
>Mature_381_residues
MIYFDNSATTAVNPMVLRTYTDVATKIMGNPSSLHGLGTTATRLLEASRRQIAELLGLDSQEIFFTSGGTEGDNWIIKGV
AFEKRPYGNHIIVSSVEHPAVKNAAEWLKTQGFELDYAPVDADGFVIVSELEKLIRDETILISVMAVNNEVGAIQPIQEI
SELLADKPTISFHVDAVQAIGKVPLETWLTDRLDFATLSAHKFHGPRGVGIVVAKKGKRLTPLLHGGGQEHNWRSTTENL
AGIAATSKALRLALDEDDFKRKKVLAMKQVILEELEKHKKVHVFSKMTDFAPNILTFGIRGGRGEVVVHAFEQHDIYIST
TSACSSKKNAAAGTLVAMNVPSKLATSAVRISLDSTNNMAEVEQFLTVFRQIYQELEKVSG

Specific function: Fe-S cluster biosynthesis [C]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=384, Percent_Identity=32.2916666666667, Blast_Score=199, Evalue=3e-51,
Organism=Homo sapiens, GI156713448, Length=414, Percent_Identity=31.1594202898551, Blast_Score=172, Evalue=4e-43,
Organism=Escherichia coli, GI48994898, Length=384, Percent_Identity=34.1145833333333, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1787970, Length=369, Percent_Identity=25.2032520325203, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1789175, Length=277, Percent_Identity=27.0758122743682, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI25143064, Length=369, Percent_Identity=33.3333333333333, Blast_Score=186, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17533177, Length=331, Percent_Identity=28.3987915407855, Blast_Score=139, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6319831, Length=365, Percent_Identity=34.5205479452055, Blast_Score=198, Evalue=1e-51,
Organism=Drosophila melanogaster, GI20129463, Length=371, Percent_Identity=35.0404312668464, Blast_Score=199, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: NA

Molecular weight: Translated: 41753; Mature: 41753

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYFDNSATTAVNPMVLRTYTDVATKIMGNPSSLHGLGTTATRLLEASRRQIAELLGLDS
CEEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHCCCCCHHHHHHHHHHHHHHHHHHCCCC
QEIFFTSGGTEGDNWIIKGVAFEKRPYGNHIIVSSVEHPAVKNAAEWLKTQGFELDYAPV
CEEEEECCCCCCCCEEEEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEECCCC
DADGFVIVSELEKLIRDETILISVMAVNNEVGAIQPIQEISELLADKPTISFHVDAVQAI
CCCCEEEHHHHHHHHCCCEEEEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEHHHHHHH
GKVPLETWLTDRLDFATLSAHKFHGPRGVGIVVAKKGKRLTPLLHGGGQEHNWRSTTENL
CCCCHHHHHHCCCCCEEECHHHCCCCCCEEEEEECCCCEECHHHCCCCCCCCCCCHHHHH
AGIAATSKALRLALDEDDFKRKKVLAMKQVILEELEKHKKVHVFSKMTDFAPNILTFGIR
HHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
GGRGEVVVHAFEQHDIYISTTSACSSKKNAAAGTLVAMNVPSKLATSAVRISLDSTNNMA
CCCCCEEEEEEECCCEEEEECCHHCCCCCCCCCEEEEECCCHHHHCEEEEEEECCCCCHH
EVEQFLTVFRQIYQELEKVSG
HHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIYFDNSATTAVNPMVLRTYTDVATKIMGNPSSLHGLGTTATRLLEASRRQIAELLGLDS
CEEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHCCCCCHHHHHHHHHHHHHHHHHHCCCC
QEIFFTSGGTEGDNWIIKGVAFEKRPYGNHIIVSSVEHPAVKNAAEWLKTQGFELDYAPV
CEEEEECCCCCCCCEEEEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEECCCC
DADGFVIVSELEKLIRDETILISVMAVNNEVGAIQPIQEISELLADKPTISFHVDAVQAI
CCCCEEEHHHHHHHHCCCEEEEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEHHHHHHH
GKVPLETWLTDRLDFATLSAHKFHGPRGVGIVVAKKGKRLTPLLHGGGQEHNWRSTTENL
CCCCHHHHHHCCCCCEEECHHHCCCCCCEEEEEECCCCEECHHHCCCCCCCCCCCHHHHH
AGIAATSKALRLALDEDDFKRKKVLAMKQVILEELEKHKKVHVFSKMTDFAPNILTFGIR
HHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
GGRGEVVVHAFEQHDIYISTTSACSSKKNAAAGTLVAMNVPSKLATSAVRISLDSTNNMA
CCCCCEEEEEEECCCEEEEECCHHCCCCCCCCCEEEEECCCHHHHCEEEEEEECCCCCHH
EVEQFLTVFRQIYQELEKVSG
HHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal Phosphate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-cysteine [C]

Specific reaction: L-cysteine = L-alanine + S [C]

General reaction: transfers sulfane sulfur to ThiI during in vitro biosynthesis of 4-thiouridine in E. coli tRNA [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8031904 [H]