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The map label for this gene is ligA

Identifier: 125623311

GI number: 125623311

Start: 434066

End: 436126

Strand: Direct

Name: ligA

Synonym: llmg_0444

Alternate gene names: 125623311

Gene position: 434066-436126 (Clockwise)

Preceding gene: 125623310

Following gene: 162329651

Centisome position: 17.16

GC content: 38.86

Gene sequence:

>2061_bases
ATGAATATCGAGTCAAAAATTAAAGAGCTTACGGATCAGCTTAATCAATTTGCCTATGAATACTATACTTTAGATGAGCC
GTCTGTAGAGGATAGTGAGTACGACCACCTATATCATGAATTAGTGAAATTGGAGCAAGAAAATCCTCAGTTAGTGAGAG
CAGATTCTCCCACTCATCGGACTGGTGGCGTCATTTTAGATGGCTTTGTGAAATTTCGACATCCTTATAATCTTTATAGT
TTAGGGGACGTTTTTTCGCGGGAAGAATTAGCCCTTTGGGAAGAACGTGTCCGCAAAGAGATTGTTAATCCAGAATACAT
TTGTGAATTAAAAATCGATGGTCTTTCGCTATCTCTTTACTATGAGAATGGCTTACTCGTGACTGCAGCAACCCGTGGTG
ATGGAACAACTGGTGAAAATATCACAGAAAATGTAAAACGAATTAAAGACGTTCCCTTAAAATTAAAAGAAGCCATTGAT
ATTGTGGTACGGGGAGAGGCTTATTTACCGCGTCAAAATTTTGCTAAACTAAATGCTGAGCGTGAACTTGAAGGAGCAGC
TCCTTTTGCCAATCCTAGAAATGCAGCAGCAGGGACTTTACGTCAGCTAGATACAAAAATCGTAGCTAAACGTGGTTTGG
CAACTTTCCTTTATCAAGAAGCAAGTCCGGCAACCAATGATACGCAAGAAGAAGTATTAGAATATTTCGAAGAACTTGGA
TTTCAAGTCAATCCTGAGCGTAAATTTGCTCGAAATTTGGATGAAATATGGGAATTTATTGAAGAAGCAACTCGCTTGCG
TGACGAATTGCCTTACGATATTGATGGTGTGGTTATCAAAGTCAATAATCTTGCTGAGCAAGAGGAATTAGGTTTTACGG
TTAAAGCCCCTCGGTGGGCTATCGCATATAAATTTCCAGCTGAACAGGCGGAAACGGAAATTTTATCTGTTGATTGGACG
GTCGGCCGTACAGGCGTTGTCACACCAACAGCAAATATGACAGCTGTTCTTTTGGCACAAACAACGGTTGCAAGGGCAAC
TTTGCATAATGTGGATTATATCCAAGAAAAAGATATCCGTAATCATGATAAAGTAATGATTTACAAGGCGGGAGATATTA
TTCCTAAAGTTGGTAAGGTCCTTCTGGAGAAGCGAAAACCAGCTAGACATCTTCGTAAAACTCATCGAGCTAACAAATAT
GTTTGGACAAGATTTGGTAGACGAAGTGTAGAGAAAGGATTAGTTTTCTCAAAGCGAGAAGATAATCGTGTAAAAATTCC
AACTCAATGTCCGGAATGTGATTCAGATTTGATCCATTTTGAGGATGAGGTGGCATTACGTTGTGTCAATCCTTTGTGTC
CTGCTCAAATCCGAGAGAAGTTAATTCATTTTGCTAGTCGTGATGCGATGAATATTGTTGGCTTGGGGCCATCAGTAATT
TCACAACTTTTTGATAAAAAATTAGTCACTGATGTTGCTGATTTATATCAATTGACCGTTGAAGATTTACTGACCCTCGA
TAAAGTCAAAGAAACTTTGGCTCAAAAAATTGTATCTGCAATTGCGCAAAGTCGTGAAAATTCAGCGGAAAAATTACTTT
TTGGTTTAGGAATTCGCCACGTCGGTGGTAAAGCTGCTAAATTATTATTAGAACGTTTTGCTAATCTTAGAGCACTCTCT
CAAGCAAGTGAAGAAGAAATTTCGGAAATTCCGTCACTTGGTGGGGTAATTGCTACAGCTGTGGTTTCATATTTTGAAAC
AGCTGGAGCCAAAACTTTACTGGATGAACTAGAAAATGCTGGTTTGAATTTTGCATATTTAGGCGCAGTTAATGTTGAAG
GGATATTATCGGGGAAGACGGTTGTTTTGACTGGTAAATTAACGACCTTGAAACGAACAGAGGCTAAAGAAAAATTGGAA
GCTTTAGGAGCAAATGTTTCGGGCTCTGTTTCTAAGAAGACTGATTTAGTGGTTGCTGGTGAAGAAGCTGGAAGTAAATT
GACAAAAGCTCAAGATTTAGGAATCGAAATCTGGTCAGAACAAGATTTACTTGATTTATAA

Upstream 100 bases:

>100_bases
TTTTTATTTTTTTATTGAATCAATTTATTAAAGAAAACTTAGAGGATTTGATGTGTTTTGGTAATCATACTAACCCGTCT
TTTGTGTTAAAATAGGAGCT

Downstream 100 bases:

>100_bases
AATAATTTATCTTGGAGTTTAGAGCAGACTTTATATCAAAAAGAGATTTACTGACAGAATTGTAAAAGCCTAAGGGCTAC
TCTCTGACAAAGCCGTCAGT

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 686; Mature: 686

Protein sequence:

>686_residues
MNIESKIKELTDQLNQFAYEYYTLDEPSVEDSEYDHLYHELVKLEQENPQLVRADSPTHRTGGVILDGFVKFRHPYNLYS
LGDVFSREELALWEERVRKEIVNPEYICELKIDGLSLSLYYENGLLVTAATRGDGTTGENITENVKRIKDVPLKLKEAID
IVVRGEAYLPRQNFAKLNAERELEGAAPFANPRNAAAGTLRQLDTKIVAKRGLATFLYQEASPATNDTQEEVLEYFEELG
FQVNPERKFARNLDEIWEFIEEATRLRDELPYDIDGVVIKVNNLAEQEELGFTVKAPRWAIAYKFPAEQAETEILSVDWT
VGRTGVVTPTANMTAVLLAQTTVARATLHNVDYIQEKDIRNHDKVMIYKAGDIIPKVGKVLLEKRKPARHLRKTHRANKY
VWTRFGRRSVEKGLVFSKREDNRVKIPTQCPECDSDLIHFEDEVALRCVNPLCPAQIREKLIHFASRDAMNIVGLGPSVI
SQLFDKKLVTDVADLYQLTVEDLLTLDKVKETLAQKIVSAIAQSRENSAEKLLFGLGIRHVGGKAAKLLLERFANLRALS
QASEEEISEIPSLGGVIATAVVSYFETAGAKTLLDELENAGLNFAYLGAVNVEGILSGKTVVLTGKLTTLKRTEAKEKLE
ALGANVSGSVSKKTDLVVAGEEAGSKLTKAQDLGIEIWSEQDLLDL

Sequences:

>Translated_686_residues
MNIESKIKELTDQLNQFAYEYYTLDEPSVEDSEYDHLYHELVKLEQENPQLVRADSPTHRTGGVILDGFVKFRHPYNLYS
LGDVFSREELALWEERVRKEIVNPEYICELKIDGLSLSLYYENGLLVTAATRGDGTTGENITENVKRIKDVPLKLKEAID
IVVRGEAYLPRQNFAKLNAERELEGAAPFANPRNAAAGTLRQLDTKIVAKRGLATFLYQEASPATNDTQEEVLEYFEELG
FQVNPERKFARNLDEIWEFIEEATRLRDELPYDIDGVVIKVNNLAEQEELGFTVKAPRWAIAYKFPAEQAETEILSVDWT
VGRTGVVTPTANMTAVLLAQTTVARATLHNVDYIQEKDIRNHDKVMIYKAGDIIPKVGKVLLEKRKPARHLRKTHRANKY
VWTRFGRRSVEKGLVFSKREDNRVKIPTQCPECDSDLIHFEDEVALRCVNPLCPAQIREKLIHFASRDAMNIVGLGPSVI
SQLFDKKLVTDVADLYQLTVEDLLTLDKVKETLAQKIVSAIAQSRENSAEKLLFGLGIRHVGGKAAKLLLERFANLRALS
QASEEEISEIPSLGGVIATAVVSYFETAGAKTLLDELENAGLNFAYLGAVNVEGILSGKTVVLTGKLTTLKRTEAKEKLE
ALGANVSGSVSKKTDLVVAGEEAGSKLTKAQDLGIEIWSEQDLLDL
>Mature_686_residues
MNIESKIKELTDQLNQFAYEYYTLDEPSVEDSEYDHLYHELVKLEQENPQLVRADSPTHRTGGVILDGFVKFRHPYNLYS
LGDVFSREELALWEERVRKEIVNPEYICELKIDGLSLSLYYENGLLVTAATRGDGTTGENITENVKRIKDVPLKLKEAID
IVVRGEAYLPRQNFAKLNAERELEGAAPFANPRNAAAGTLRQLDTKIVAKRGLATFLYQEASPATNDTQEEVLEYFEELG
FQVNPERKFARNLDEIWEFIEEATRLRDELPYDIDGVVIKVNNLAEQEELGFTVKAPRWAIAYKFPAEQAETEILSVDWT
VGRTGVVTPTANMTAVLLAQTTVARATLHNVDYIQEKDIRNHDKVMIYKAGDIIPKVGKVLLEKRKPARHLRKTHRANKY
VWTRFGRRSVEKGLVFSKREDNRVKIPTQCPECDSDLIHFEDEVALRCVNPLCPAQIREKLIHFASRDAMNIVGLGPSVI
SQLFDKKLVTDVADLYQLTVEDLLTLDKVKETLAQKIVSAIAQSRENSAEKLLFGLGIRHVGGKAAKLLLERFANLRALS
QASEEEISEIPSLGGVIATAVVSYFETAGAKTLLDELENAGLNFAYLGAVNVEGILSGKTVVLTGKLTTLKRTEAKEKLE
ALGANVSGSVSKKTDLVVAGEEAGSKLTKAQDLGIEIWSEQDLLDL

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=699, Percent_Identity=42.6323319027182, Blast_Score=524, Evalue=1e-150,
Organism=Escherichia coli, GI87082305, Length=608, Percent_Identity=21.5460526315789, Blast_Score=110, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_LACLM (A2RIF3)

Other databases:

- EMBL:   AM406671
- RefSeq:   YP_001031794.1
- ProteinModelPortal:   A2RIF3
- STRING:   A2RIF3
- GeneID:   4798183
- GenomeReviews:   AM406671_GR
- KEGG:   llm:llmg_0444
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   PRK07956
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 76775; Mature: 76775

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 111-111 BINDING 109-109 BINDING 132-132 BINDING 166-166 BINDING 280-280 BINDING 304-304

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIESKIKELTDQLNQFAYEYYTLDEPSVEDSEYDHLYHELVKLEQENPQLVRADSPTHR
CCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCC
TGGVILDGFVKFRHPYNLYSLGDVFSREELALWEERVRKEIVNPEYICELKIDGLSLSLY
CCCEEEECHHHHCCCCCCHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEEEE
YENGLLVTAATRGDGTTGENITENVKRIKDVPLKLKEAIDIVVRGEAYLPRQNFAKLNAE
EECCEEEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHEEEEEECCCCCCCHHHHHCCCC
RELEGAAPFANPRNAAAGTLRQLDTKIVAKRGLATFLYQEASPATNDTQEEVLEYFEELG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCC
FQVNPERKFARNLDEIWEFIEEATRLRDELPYDIDGVVIKVNNLAEQEELGFTVKAPRWA
CEECCHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHCCEEEECCCEE
IAYKFPAEQAETEILSVDWTVGRTGVVTPTANMTAVLLAQTTVARATLHNVDYIQEKDIR
EEEECCCHHHCCEEEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCC
NHDKVMIYKAGDIIPKVGKVLLEKRKPARHLRKTHRANKYVWTRFGRRSVEKGLVFSKRE
CCCEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCEEECCC
DNRVKIPTQCPECDSDLIHFEDEVALRCVNPLCPAQIREKLIHFASRDAMNIVGLGPSVI
CCEEECCCCCCCCCCCCEECCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEECCHHHH
SQLFDKKLVTDVADLYQLTVEDLLTLDKVKETLAQKIVSAIAQSRENSAEKLLFGLGIRH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCHHH
VGGKAAKLLLERFANLRALSQASEEEISEIPSLGGVIATAVVSYFETAGAKTLLDELENA
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHC
GLNFAYLGAVNVEGILSGKTVVLTGKLTTLKRTEAKEKLEALGANVSGSVSKKTDLVVAG
CCCEEEEECCCCCEEECCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEC
EEAGSKLTKAQDLGIEIWSEQDLLDL
CHHCCHHHHHHHCCEEEECCCCCCCC
>Mature Secondary Structure
MNIESKIKELTDQLNQFAYEYYTLDEPSVEDSEYDHLYHELVKLEQENPQLVRADSPTHR
CCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCC
TGGVILDGFVKFRHPYNLYSLGDVFSREELALWEERVRKEIVNPEYICELKIDGLSLSLY
CCCEEEECHHHHCCCCCCHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEEEE
YENGLLVTAATRGDGTTGENITENVKRIKDVPLKLKEAIDIVVRGEAYLPRQNFAKLNAE
EECCEEEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHEEEEEECCCCCCCHHHHHCCCC
RELEGAAPFANPRNAAAGTLRQLDTKIVAKRGLATFLYQEASPATNDTQEEVLEYFEELG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCC
FQVNPERKFARNLDEIWEFIEEATRLRDELPYDIDGVVIKVNNLAEQEELGFTVKAPRWA
CEECCHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHCCEEEECCCEE
IAYKFPAEQAETEILSVDWTVGRTGVVTPTANMTAVLLAQTTVARATLHNVDYIQEKDIR
EEEECCCHHHCCEEEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCC
NHDKVMIYKAGDIIPKVGKVLLEKRKPARHLRKTHRANKYVWTRFGRRSVEKGLVFSKRE
CCCEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCEEECCC
DNRVKIPTQCPECDSDLIHFEDEVALRCVNPLCPAQIREKLIHFASRDAMNIVGLGPSVI
CCEEECCCCCCCCCCCCEECCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEECCHHHH
SQLFDKKLVTDVADLYQLTVEDLLTLDKVKETLAQKIVSAIAQSRENSAEKLLFGLGIRH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCHHH
VGGKAAKLLLERFANLRALSQASEEEISEIPSLGGVIATAVVSYFETAGAKTLLDELENA
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHC
GLNFAYLGAVNVEGILSGKTVVLTGKLTTLKRTEAKEKLEALGANVSGSVSKKTDLVVAG
CCCEEEEECCCCCEEECCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEC
EEAGSKLTKAQDLGIEIWSEQDLLDL
CHHCCHHHHHHHCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA