BacMap

Definition	Lactococcus lactis subsp. cremoris MG1363, complete genome.
Accession	NC_009004
Length	2,529,478

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The map label for this gene is dar [H]

Identifier: 125623209

GI number: 125623209

Start: 326229

End: 326999

Strand: Direct

Name: dar [H]

Synonym: llmg_0339

Alternate gene names: 125623209

Gene position: 326229-326999 (Clockwise)

Preceding gene: 125623208

Following gene: 125623210

Centisome position: 12.9

GC content: 40.21

Gene sequence:

>771_bases
ATGGGGAAATTAGCGATTGTAACGGGAAGCGGACAAGGTATTGGGGAAGGTATTGTCTATCGGTTAGCAAAAGATGGATT
TGCGGTTGCCGTAGCAGATATTAATCCTCAAACAGCAAAAAATGTTGCAAAAACTTTGATAGATAAAGGATTTGAAGCTA
AAGCTTACGTGCTTGATGTTGCGAACCGTTCGGCAGTTTTTCAGCTTATTGAACAAGCGGTGAGTGATTTTGGTGAACTT
GCTGTATTTGTTAATAATGCTGGGGTTGCTTTTATTGATAGCATTATAGATTCTGATTCAGAAAGGGTTGAACGACTATT
TGATGTCAATATCAAAGGTACTTATTGGGGAATACAAGCAGCAGCTACCCAATTTAAAAAACAAGGTCATGGCGGACGAA
TTATCAATGCGGCTTCGCTTGCAAGTGTTGAAGGTTCTGCCTTGCAAAGTGCTTATTCTGCTTCAAAATTTGCAATACGT
GGATTGACACAGTCGGCAGCGAAAGAACTTGCAAAAGACTATATCACAGTGAATGCTTATAATCCTGGAATTGTACGCAC
CGCTATGCGAGATGAGATTGATAAAAAAACGGCTCAAATCAAAGGAATTAGCATTGAACAGCAACAACAGAATTGTTTGA
ATGAAATCTACCTCGGACGTGAAGGAAGACCAGAAGATGTTGCAGAAGTTGTGGCCTGGTTTGCTTCAGATGCAGCAGAA
TATATTACAGGACAATCTATCTTAGTGGATGGCGGGATGAGATTCAATTAG

Upstream 100 bases:

>100_bases
GAGAATTGCCCGCTTGGGACTTGAAACTTTGAATAAATACGCTTCAGCAGCTGAGGACAAAAAATTATTAAGCTCCTAGA
AATTGTGAAAGAAGAAAAAT

Downstream 100 bases:

>100_bases
CAAGAAGGAAAAAATATGAATGTAAAAAATCGGAGAATATTAATTACGATTATTATTCTTGTATTCATTATTATAGTAGG
CGGGATTTTTGCCTTTTCTC

Product: acetoin(diacetyl)reductase

Products: NA

Alternate protein names: Acetoin(diacetyl) reductase; AR; Meso-2,3-butanediol dehydrogenase [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MGKLAIVTGSGQGIGEGIVYRLAKDGFAVAVADINPQTAKNVAKTLIDKGFEAKAYVLDVANRSAVFQLIEQAVSDFGEL
AVFVNNAGVAFIDSIIDSDSERVERLFDVNIKGTYWGIQAAATQFKKQGHGGRIINAASLASVEGSALQSAYSASKFAIR
GLTQSAAKELAKDYITVNAYNPGIVRTAMRDEIDKKTAQIKGISIEQQQQNCLNEIYLGREGRPEDVAEVVAWFASDAAE
YITGQSILVDGGMRFN

Sequences:

>Translated_256_residues
MGKLAIVTGSGQGIGEGIVYRLAKDGFAVAVADINPQTAKNVAKTLIDKGFEAKAYVLDVANRSAVFQLIEQAVSDFGEL
AVFVNNAGVAFIDSIIDSDSERVERLFDVNIKGTYWGIQAAATQFKKQGHGGRIINAASLASVEGSALQSAYSASKFAIR
GLTQSAAKELAKDYITVNAYNPGIVRTAMRDEIDKKTAQIKGISIEQQQQNCLNEIYLGREGRPEDVAEVVAWFASDAAE
YITGQSILVDGGMRFN
>Mature_255_residues
GKLAIVTGSGQGIGEGIVYRLAKDGFAVAVADINPQTAKNVAKTLIDKGFEAKAYVLDVANRSAVFQLIEQAVSDFGELA
VFVNNAGVAFIDSIIDSDSERVERLFDVNIKGTYWGIQAAATQFKKQGHGGRIINAASLASVEGSALQSAYSASKFAIRG
LTQSAAKELAKDYITVNAYNPGIVRTAMRDEIDKKTAQIKGISIEQQQQNCLNEIYLGREGRPEDVAEVVAWFASDAAEY
ITGQSILVDGGMRFN

Specific function: Catalyzes the reversible reduction of (S)-acetoin to 2,3-butanediol in the presence of NADH [H]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI15277342, Length=258, Percent_Identity=34.1085271317829, Blast_Score=129, Evalue=2e-30,
Organism=Homo sapiens, GI32483357, Length=252, Percent_Identity=32.1428571428571, Blast_Score=123, Evalue=1e-28,
Organism=Homo sapiens, GI40254992, Length=255, Percent_Identity=30.5882352941176, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI210032110, Length=190, Percent_Identity=31.5789473684211, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI31542939, Length=204, Percent_Identity=35.2941176470588, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI19923817, Length=258, Percent_Identity=29.4573643410853, Blast_Score=95, Evalue=6e-20,
Organism=Homo sapiens, GI126723750, Length=255, Percent_Identity=27.843137254902, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI142976729, Length=189, Percent_Identity=28.5714285714286, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI7705925, Length=251, Percent_Identity=28.2868525896414, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI5031737, Length=250, Percent_Identity=28, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI66933014, Length=252, Percent_Identity=29.7619047619048, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI224922801, Length=181, Percent_Identity=34.8066298342541, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI4758504, Length=265, Percent_Identity=28.3018867924528, Blast_Score=84, Evalue=9e-17,
Organism=Homo sapiens, GI40807363, Length=194, Percent_Identity=28.3505154639175, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI25282469, Length=234, Percent_Identity=25.6410256410256, Blast_Score=81, Evalue=8e-16,
Organism=Homo sapiens, GI126723191, Length=187, Percent_Identity=32.0855614973262, Blast_Score=81, Evalue=8e-16,
Organism=Homo sapiens, GI109715829, Length=245, Percent_Identity=25.7142857142857, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI310128830, Length=218, Percent_Identity=26.605504587156, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI310115468, Length=218, Percent_Identity=26.605504587156, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI10190704, Length=252, Percent_Identity=25.3968253968254, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI83715985, Length=265, Percent_Identity=27.1698113207547, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI33667109, Length=186, Percent_Identity=29.0322580645161, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI40254986, Length=202, Percent_Identity=25.7425742574257, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI4504505, Length=255, Percent_Identity=26.2745098039216, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI4503817, Length=210, Percent_Identity=30.4761904761905, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI59889578, Length=232, Percent_Identity=26.7241379310345, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI32455239, Length=231, Percent_Identity=29.004329004329, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI5031765, Length=231, Percent_Identity=29.004329004329, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI225579078, Length=203, Percent_Identity=27.5862068965517, Blast_Score=65, Evalue=8e-11,
Organism=Homo sapiens, GI223718074, Length=241, Percent_Identity=26.5560165975104, Blast_Score=64, Evalue=1e-10,
Organism=Escherichia coli, GI1788459, Length=256, Percent_Identity=33.203125, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI1789208, Length=251, Percent_Identity=33.4661354581673, Blast_Score=131, Evalue=6e-32,
Organism=Escherichia coli, GI87082100, Length=259, Percent_Identity=35.1351351351351, Blast_Score=130, Evalue=8e-32,
Organism=Escherichia coli, GI1787335, Length=253, Percent_Identity=31.6205533596838, Blast_Score=120, Evalue=7e-29,
Organism=Escherichia coli, GI1789057, Length=255, Percent_Identity=33.3333333333333, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI2367175, Length=256, Percent_Identity=32.03125, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI2367365, Length=255, Percent_Identity=30.1960784313725, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI87082160, Length=254, Percent_Identity=30.7086614173228, Blast_Score=97, Evalue=9e-22,
Organism=Escherichia coli, GI1786812, Length=260, Percent_Identity=31.1538461538462, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1790717, Length=252, Percent_Identity=26.984126984127, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1787905, Length=251, Percent_Identity=29.4820717131474, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1789378, Length=255, Percent_Identity=27.843137254902, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1787820, Length=238, Percent_Identity=26.0504201680672, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787526, Length=192, Percent_Identity=27.0833333333333, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17561402, Length=256, Percent_Identity=32.421875, Blast_Score=118, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI25147288, Length=257, Percent_Identity=33.4630350194552, Blast_Score=113, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17555706, Length=251, Percent_Identity=31.8725099601594, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17538480, Length=266, Percent_Identity=30.0751879699248, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17551412, Length=268, Percent_Identity=29.8507462686567, Blast_Score=104, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17560676, Length=257, Percent_Identity=30.3501945525292, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17560220, Length=261, Percent_Identity=29.8850574712644, Blast_Score=100, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17531453, Length=262, Percent_Identity=28.6259541984733, Blast_Score=98, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17538182, Length=265, Percent_Identity=30.188679245283, Blast_Score=97, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17562906, Length=267, Percent_Identity=29.2134831460674, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17560332, Length=273, Percent_Identity=30.03663003663, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17562908, Length=265, Percent_Identity=29.4339622641509, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI115534694, Length=256, Percent_Identity=30.859375, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17563726, Length=263, Percent_Identity=28.8973384030418, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17562910, Length=263, Percent_Identity=29.6577946768061, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17560150, Length=261, Percent_Identity=29.1187739463602, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17508651, Length=253, Percent_Identity=30.8300395256917, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17562990, Length=261, Percent_Identity=28.735632183908, Blast_Score=90, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI193204405, Length=263, Percent_Identity=29.277566539924, Blast_Score=90, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17544670, Length=273, Percent_Identity=27.4725274725275, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71994600, Length=254, Percent_Identity=25.9842519685039, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17538486, Length=263, Percent_Identity=27.7566539923954, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17508895, Length=192, Percent_Identity=29.1666666666667, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17559104, Length=265, Percent_Identity=27.5471698113208, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17565030, Length=269, Percent_Identity=28.6245353159851, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17562904, Length=263, Percent_Identity=29.277566539924, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI72000259, Length=267, Percent_Identity=27.3408239700375, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71994604, Length=258, Percent_Identity=25.1937984496124, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17567345, Length=191, Percent_Identity=27.7486910994764, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI193203115, Length=219, Percent_Identity=27.3972602739726, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI193203117, Length=219, Percent_Identity=27.3972602739726, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17568967, Length=193, Percent_Identity=32.6424870466321, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17536025, Length=278, Percent_Identity=29.136690647482, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17564282, Length=226, Percent_Identity=24.3362831858407, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17536651, Length=267, Percent_Identity=26.2172284644195, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71982365, Length=191, Percent_Identity=25.130890052356, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17558628, Length=190, Percent_Identity=27.3684210526316, Blast_Score=64, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=206, Percent_Identity=31.5533980582524, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324126, Length=261, Percent_Identity=25.2873563218391, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323882, Length=192, Percent_Identity=31.25, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322227, Length=210, Percent_Identity=29.5238095238095, Blast_Score=65, Evalue=9e-12,
Organism=Drosophila melanogaster, GI21355319, Length=261, Percent_Identity=34.8659003831418, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24639444, Length=256, Percent_Identity=30.46875, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI23397609, Length=250, Percent_Identity=31.6, Blast_Score=107, Evalue=6e-24,
Organism=Drosophila melanogaster, GI28571526, Length=256, Percent_Identity=31.25, Blast_Score=104, Evalue=5e-23,
Organism=Drosophila melanogaster, GI281364892, Length=215, Percent_Identity=30.6976744186047, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24644339, Length=254, Percent_Identity=29.1338582677165, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21357041, Length=253, Percent_Identity=30.0395256916996, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24643142, Length=252, Percent_Identity=30.952380952381, Blast_Score=94, Evalue=8e-20,
Organism=Drosophila melanogaster, GI17737361, Length=264, Percent_Identity=29.5454545454545, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24640492, Length=169, Percent_Identity=27.810650887574, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24649181, Length=191, Percent_Identity=31.9371727748691, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI18859837, Length=251, Percent_Identity=29.4820717131474, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24644337, Length=252, Percent_Identity=25.3968253968254, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24581726, Length=192, Percent_Identity=25, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI21357673, Length=198, Percent_Identity=27.2727272727273, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI221510726, Length=167, Percent_Identity=29.940119760479, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21358495, Length=233, Percent_Identity=23.6051502145923, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI21355631, Length=198, Percent_Identity=27.7777777777778, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI45554248, Length=220, Percent_Identity=28.1818181818182, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24640117, Length=220, Percent_Identity=28.1818181818182, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24586328, Length=200, Percent_Identity=30, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014007
- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.1.1.304 [H]

Molecular weight: Translated: 27436; Mature: 27305

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKLAIVTGSGQGIGEGIVYRLAKDGFAVAVADINPQTAKNVAKTLIDKGFEAKAYVLDV
CCCEEEEECCCCCCCHHHHHHEECCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEEC
ANRSAVFQLIEQAVSDFGELAVFVNNAGVAFIDSIIDSDSERVERLFDVNIKGTYWGIQA
CCHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHCCCHHHHHHHHCCCCCCEEHHHHH
AATQFKKQGHGGRIINAASLASVEGSALQSAYSASKFAIRGLTQSAAKELAKDYITVNAY
HHHHHHHCCCCCEEEEHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECC
NPGIVRTAMRDEIDKKTAQIKGISIEQQQQNCLNEIYLGREGRPEDVAEVVAWFASDAAE
CCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
YITGQSILVDGGMRFN
HHCCCEEEEECCCCCC
>Mature Secondary Structure 
GKLAIVTGSGQGIGEGIVYRLAKDGFAVAVADINPQTAKNVAKTLIDKGFEAKAYVLDV
CCEEEEECCCCCCCHHHHHHEECCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEEC
ANRSAVFQLIEQAVSDFGELAVFVNNAGVAFIDSIIDSDSERVERLFDVNIKGTYWGIQA
CCHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHCCCHHHHHHHHCCCCCCEEHHHHH
AATQFKKQGHGGRIINAASLASVEGSALQSAYSASKFAIRGLTQSAAKELAKDYITVNAY
HHHHHHHCCCCCEEEEHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECC
NPGIVRTAMRDEIDKKTAQIKGISIEQQQQNCLNEIYLGREGRPEDVAEVVAWFASDAAE
CCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
YITGQSILVDGGMRFN
HHCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11173520 [H]