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The map label for this gene is dhaK [H]

Identifier: 125623131

GI number: 125623131

Start: 241721

End: 242707

Strand: Direct

Name: dhaK [H]

Synonym: llmg_0255

Alternate gene names: 125623131

Gene position: 241721-242707 (Clockwise)

Preceding gene: 125623130

Following gene: 125623132

Centisome position: 9.56

GC content: 37.49

Gene sequence:

>987_bases
ATGAAATTCTATACTTCAACGACAGAAATTCCAGAAGAAATGTTAAAAGGAGTGGAGTTAACTCACACTCATTTGTCCTA
TCTTTCAGAAACTGGAATTCTCTATGATAATACATTAGATAATAAGGCAGTTCCAATTATTTCAGGAGGTGGAAGTGGTC
ATGAACCCGCTCACATTGGTTATGTTGGGGCTGGGATGCTTGCTGCAGCTGTGACTGGCCCTCTATTTGTACCGCCAAGA
GCTAAAAATATTTTAAAAGCCATTCGTCAAGTTAATTCAGGCAAAGGTGTTTTTGTTATTATCAAAAATTTCGAAGCTGA
TTTAAAAGAGTTTAAAAAAGCGATTGAAGAGGCAAGAACTGAAGGGATTGACGTCAGATACATTGTCAGCCATGATGATA
TTTCGGTAAATACTTATAATTTTCATAAGAGACATCGGGGAGTCGCTGGAACGATTTTGCTTCATAAAGTACTCGGTGCT
TTTGCAAAAGAAGGGGCAAGTATTGATGAAATTGAACAATTGGCTCTGTCCCTTTCCCCAGAAATTTATACTTTGGGTGT
CGCCTTAGCTCCAGTTAATTTTCCTCATCATAGGACCTCCTTTCTATTAGCAGAAGATGAAGTGTCTTTTGGCGTTGGAA
TTCATGGCGAGCCTGGTTACCGAATCGAAAAATTTGAAGGCTCTGAGCGTATTGCCGTAGAATTAGTAAATAAGTTAAAA
GCAGAAATTAATTGGCAAAAAAAAGCCAATAAAAATTATATTTTGCTTGTAAATGGTCTTGGTTCAACGACTTTAATGGA
ACTCTATCATTTTCAATACGATGTCATGCGCCTTTTAGAACTCGAAGGATTGTCTATTAAGTTTTGCAAAGTTGGAAACC
TAATGACAAGTTGTGATATGTCAGGAATTTCATTAACACTTTGTTCTGTCAAAGATCCTAAATGGTTGGACTATCTGAAT
GCTCCAACTGGAGCTTTCGCCTGGTAA

Upstream 100 bases:

>100_bases
TTAGCAGCTTCACAAGATCAAAATTTTCTGTTGAGATAAATAAAAAGAGTTTGTTTTTTAAAATCAAGCTCTTTTTGTAT
CTGTGCTATAATTTGATTAT

Downstream 100 bases:

>100_bases
TCAATCTTCAAAAAGATTCAGTTGAGCGAGTAAGAGTTTAACTATCTGAGGATAAAGCTCCTCTAAAGAATTTCCTCTGA
TGATTGACTCTTTAGTTATT

Product: DhaKLM operon coactivator DhaQ

Products: NA

Alternate protein names: DhaS coactivator dhaQ [H]

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MKFYTSTTEIPEEMLKGVELTHTHLSYLSETGILYDNTLDNKAVPIISGGGSGHEPAHIGYVGAGMLAAAVTGPLFVPPR
AKNILKAIRQVNSGKGVFVIIKNFEADLKEFKKAIEEARTEGIDVRYIVSHDDISVNTYNFHKRHRGVAGTILLHKVLGA
FAKEGASIDEIEQLALSLSPEIYTLGVALAPVNFPHHRTSFLLAEDEVSFGVGIHGEPGYRIEKFEGSERIAVELVNKLK
AEINWQKKANKNYILLVNGLGSTTLMELYHFQYDVMRLLELEGLSIKFCKVGNLMTSCDMSGISLTLCSVKDPKWLDYLN
APTGAFAW

Sequences:

>Translated_328_residues
MKFYTSTTEIPEEMLKGVELTHTHLSYLSETGILYDNTLDNKAVPIISGGGSGHEPAHIGYVGAGMLAAAVTGPLFVPPR
AKNILKAIRQVNSGKGVFVIIKNFEADLKEFKKAIEEARTEGIDVRYIVSHDDISVNTYNFHKRHRGVAGTILLHKVLGA
FAKEGASIDEIEQLALSLSPEIYTLGVALAPVNFPHHRTSFLLAEDEVSFGVGIHGEPGYRIEKFEGSERIAVELVNKLK
AEINWQKKANKNYILLVNGLGSTTLMELYHFQYDVMRLLELEGLSIKFCKVGNLMTSCDMSGISLTLCSVKDPKWLDYLN
APTGAFAW
>Mature_328_residues
MKFYTSTTEIPEEMLKGVELTHTHLSYLSETGILYDNTLDNKAVPIISGGGSGHEPAHIGYVGAGMLAAAVTGPLFVPPR
AKNILKAIRQVNSGKGVFVIIKNFEADLKEFKKAIEEARTEGIDVRYIVSHDDISVNTYNFHKRHRGVAGTILLHKVLGA
FAKEGASIDEIEQLALSLSPEIYTLGVALAPVNFPHHRTSFLLAEDEVSFGVGIHGEPGYRIEKFEGSERIAVELVNKLK
AEINWQKKANKNYILLVNGLGSTTLMELYHFQYDVMRLLELEGLSIKFCKVGNLMTSCDMSGISLTLCSVKDPKWLDYLN
APTGAFAW

Specific function: Coactivator for the transcription factor dhaS. The heterotetramer formed by dhaQ and dhaS functions as transcriptional regulator. Activated by covalent binding of dihydroxyacetone to dhaQ. The complex activates the dhaKLM operon [H]

COG id: COG2376

COG function: function code G; Dihydroxyacetone kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DhaK domain [H]

Homologues:

Organism=Homo sapiens, GI20149621, Length=334, Percent_Identity=35.0299401197605, Blast_Score=190, Evalue=2e-48,
Organism=Escherichia coli, GI87081857, Length=355, Percent_Identity=33.5211267605634, Blast_Score=187, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17565018, Length=285, Percent_Identity=34.0350877192982, Blast_Score=171, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6323570, Length=340, Percent_Identity=32.9411764705882, Blast_Score=156, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6321055, Length=303, Percent_Identity=30.6930693069307, Blast_Score=144, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004006
- InterPro:   IPR012735 [H]

Pfam domain/function: PF02733 Dak1 [H]

EC number: NA

Molecular weight: Translated: 36169; Mature: 36169

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFYTSTTEIPEEMLKGVELTHTHLSYLSETGILYDNTLDNKAVPIISGGGSGHEPAHIG
CCEECCCHHHHHHHHCCCCHHHHHHHHHHHCCCEEECCCCCCCCEEEECCCCCCCCCEEE
YVGAGMLAAAVTGPLFVPPRAKNILKAIRQVNSGKGVFVIIKNFEADLKEFKKAIEEART
EHHHHHHHHHHCCCEECCCCHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHH
EGIDVRYIVSHDDISVNTYNFHKRHRGVAGTILLHKVLGAFAKEGASIDEIEQLALSLSP
CCCCEEEEEECCCEEEEEEEHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC
EIYTLGVALAPVNFPHHRTSFLLAEDEVSFGVGIHGEPGYRIEKFEGSERIAVELVNKLK
CEEEEEEEEECCCCCCCCCEEEEEECCCEECEEECCCCCCEEEECCCCCHHHHHHHHHHH
AEINWQKKANKNYILLVNGLGSTTLMELYHFQYDVMRLLELEGLSIKFCKVGNLMTSCDM
HHCCCHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHCCC
SGISLTLCSVKDPKWLDYLNAPTGAFAW
CCCEEEEEECCCCHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKFYTSTTEIPEEMLKGVELTHTHLSYLSETGILYDNTLDNKAVPIISGGGSGHEPAHIG
CCEECCCHHHHHHHHCCCCHHHHHHHHHHHCCCEEECCCCCCCCEEEECCCCCCCCCEEE
YVGAGMLAAAVTGPLFVPPRAKNILKAIRQVNSGKGVFVIIKNFEADLKEFKKAIEEART
EHHHHHHHHHHCCCEECCCCHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHH
EGIDVRYIVSHDDISVNTYNFHKRHRGVAGTILLHKVLGAFAKEGASIDEIEQLALSLSP
CCCCEEEEEECCCEEEEEEEHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC
EIYTLGVALAPVNFPHHRTSFLLAEDEVSFGVGIHGEPGYRIEKFEGSERIAVELVNKLK
CEEEEEEEEECCCCCCCCCEEEEEECCCEECEEECCCCCCEEEECCCCCHHHHHHHHHHH
AEINWQKKANKNYILLVNGLGSTTLMELYHFQYDVMRLLELEGLSIKFCKVGNLMTSCDM
HHCCCHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHCCC
SGISLTLCSVKDPKWLDYLNAPTGAFAW
CCCEEEEEECCCCHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]