Click here to switch to the map view.

The map label for this gene is cspE [H]

Identifier: 125623057

GI number: 125623057

Start: 170956

End: 171153

Strand: Reverse

Name: cspE [H]

Synonym: llmg_0180

Alternate gene names: 125623057

Gene position: 171153-170956 (Counterclockwise)

Preceding gene: 125623075

Following gene: 125623056

Centisome position: 6.77

GC content: 40.91

Gene sequence:

>198_bases
ATGGCACAAGGAACTGTTAAATGGTTTAACGCAACTAAAGGCTTCGGCTTTATCACTACTGAAGAAGGAAACGACGTTTT
CGCACACTTCTCAGCTATCCAAACTGATGGATTCAAAACACTTGACGAAGGTCAAAAAGTTACTTTTGACGTTGAAGAAG
GCCCTCGTGGACCTCAAGCTGTTAACATCCAAAAATAA

Upstream 100 bases:

>100_bases
AAAGTGTAAGTTTTTGTTTTACGATTAGTTCTGTGCTTTTGGGTTCATTCCTATTCAAGAAATTAGATTTACAAATTTAT
AAAATAAGGAAGTACCAAAA

Downstream 100 bases:

>100_bases
TTAAAGCTTTGAGGGTCACTGACAAAATTGTCAGTGACTTTTTTTGCGCCATTTTTTTACTGACAAACTTTTTATATGAA
AGCCTTAACAAAAAAATGCT

Product: cold shock-like protein cspE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 65; Mature: 64

Protein sequence:

>65_residues
MAQGTVKWFNATKGFGFITTEEGNDVFAHFSAIQTDGFKTLDEGQKVTFDVEEGPRGPQAVNIQK

Sequences:

>Translated_65_residues
MAQGTVKWFNATKGFGFITTEEGNDVFAHFSAIQTDGFKTLDEGQKVTFDVEEGPRGPQAVNIQK
>Mature_64_residues
AQGTVKWFNATKGFGFITTEEGNDVFAHFSAIQTDGFKTLDEGQKVTFDVEEGPRGPQAVNIQK

Specific function: Binds To And Stimulates The Transcription Of The CCAAT- Containing, Cold-Shock-Inducible Promoters Of The H-Ns And Gyra Proteins. Binds Also To The Inverted Repeat 5'-ATTGG-3'. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1789979, Length=61, Percent_Identity=67.2131147540984, Blast_Score=88, Evalue=1e-19,
Organism=Escherichia coli, GI1786841, Length=62, Percent_Identity=64.5161290322581, Blast_Score=86, Evalue=4e-19,
Organism=Escherichia coli, GI1787839, Length=61, Percent_Identity=63.9344262295082, Blast_Score=83, Evalue=4e-18,
Organism=Escherichia coli, GI2367114, Length=61, Percent_Identity=62.2950819672131, Blast_Score=82, Evalue=6e-18,
Organism=Escherichia coli, GI1788126, Length=62, Percent_Identity=62.9032258064516, Blast_Score=82, Evalue=7e-18,
Organism=Escherichia coli, GI1787834, Length=61, Percent_Identity=62.2950819672131, Blast_Score=80, Evalue=2e-17,
Organism=Escherichia coli, GI1787107, Length=64, Percent_Identity=59.375, Blast_Score=79, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7138; Mature: 7007

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQGTVKWFNATKGFGFITTEEGNDVFAHFSAIQTDGFKTLDEGQKVTFDVEEGPRGPQA
CCCCCEEEEECCCCCEEEEECCCCEEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCE
VNIQK
EEECC
>Mature Secondary Structure 
AQGTVKWFNATKGFGFITTEEGNDVFAHFSAIQTDGFKTLDEGQKVTFDVEEGPRGPQA
CCCCEEEEECCCCCEEEEECCCCEEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCE
VNIQK
EEECC

PDB accession: NA

Resolution: NA

Structure class: Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]