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The map label for this gene is tgt [H]

Identifier: 125623043

GI number: 125623043

Start: 154740

End: 155888

Strand: Reverse

Name: tgt [H]

Synonym: llmg_0164

Alternate gene names: 125623043

Gene position: 155888-154740 (Counterclockwise)

Preceding gene: 125623045

Following gene: 125623038

Centisome position: 6.16

GC content: 40.91

Gene sequence:

>1149_bases
ATGACAGAACACGCAATAAAATACAGATTAATAAAAAAAGAAAAACACACTGGAGCAAGATTGGGAGAAATCATTACCCC
TCACGGAACCTTCCCAACTCCAATGTTCATGCCAGTCGGTACCCAAGCCACTGTAAAAACAATGAGCCCAGAAGAACTCA
AAACTCTCGGCTCTGGAATCATTCTCTCAAATACCTACCACCTTTGGCTACGCCCTGGTGACGAACTCGTTGCTGAAGCT
GGTGGTCTTCACAAATTCATGAATTGGGACCAACCCATCCTAACAGATAGTGGTGGTTTCCAAGTTTATAGTCTTGTTCA
AAATAAGAAAAATATTACAGAAGAAGGCGTTAAATTTAAAAGCCATTTAGATGGACGTGAACTTTTTCTCAATCCAGAAA
AAGCAATTAGTATCCAAAACAATCTTGGTTCAGATATCATGATGTCCTTTGATGAATGCCCACCTTTTTACCAACCTTAT
GATTACGTTAAAGCCTCTGTTGAACGAACTTCACGATGGGCTGAACGTGGGCTCAATGCTCACCGCCGTCCAAATGACCA
AGGACTGTTTGGAATTGTTCAGGGAGCTGGCTTTGAAGATTTACGTCGTCAATCAGCTAGCGATTTAACTTCTATGGATT
TCGCTGGTTATTCGATCGGTGGTCTTGCCGTTGGAGAATCACATAAAGAAATGAACGCTGTTCTTGACTTTACAACACCA
ATGCTTCCTGAAGATAAACCACGCTATCTAATGGGTGTTGGTGCTCCAGATAGCTTGATTGATGGAGTTATACGTGGGGT
GGATATGTTTGACTGTGTTCTTCCTACACGAATCGCTCGTAACGGAACACTAATGACACATTTTGGTCGAGTAAATATTC
GAAATGCTAAATATGAGTATGATTTCACTCCACTTGACCCAATGTGTGATTGCTACACTTGTACCAACTACACACGCGCC
TATCTACGTCATTTAATTAAAGCCGATGAAACCTTTGGTTTACGCCTTTGCTCATACCATAATCTTCATTTCCTAGTAAA
CCTCATGAAAGATGTTCGCCAAGCAATCATGGATGATAACCTTCTAGAATTCCGTGAAGATTTTTGTGAACGTTATGGCT
ACAATCAACCCAATGCCAAAGATTTTTAG

Upstream 100 bases:

>100_bases
AATCCATTTAATTTTTGTTATACTTATAAGTGCTTGCTTTTTAAGCCACAAGAAAGCTCAACTCGAAAATTAAAAAAAAC
AAGTAAATTAAAGGAATTTT

Downstream 100 bases:

>100_bases
TTCTTGAAAAAGCAATTAAAAAGTTGTATTATAATAAAAGTGGAGAACCACTTTTATTTTGTGCCGACTTAGCTCAGTTG
GTAGAGCACTTCACTCGTAA

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MTEHAIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQATVKTMSPEELKTLGSGIILSNTYHLWLRPGDELVAEA
GGLHKFMNWDQPILTDSGGFQVYSLVQNKKNITEEGVKFKSHLDGRELFLNPEKAISIQNNLGSDIMMSFDECPPFYQPY
DYVKASVERTSRWAERGLNAHRRPNDQGLFGIVQGAGFEDLRRQSASDLTSMDFAGYSIGGLAVGESHKEMNAVLDFTTP
MLPEDKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTRIARNGTLMTHFGRVNIRNAKYEYDFTPLDPMCDCYTCTNYTRA
YLRHLIKADETFGLRLCSYHNLHFLVNLMKDVRQAIMDDNLLEFREDFCERYGYNQPNAKDF

Sequences:

>Translated_382_residues
MTEHAIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQATVKTMSPEELKTLGSGIILSNTYHLWLRPGDELVAEA
GGLHKFMNWDQPILTDSGGFQVYSLVQNKKNITEEGVKFKSHLDGRELFLNPEKAISIQNNLGSDIMMSFDECPPFYQPY
DYVKASVERTSRWAERGLNAHRRPNDQGLFGIVQGAGFEDLRRQSASDLTSMDFAGYSIGGLAVGESHKEMNAVLDFTTP
MLPEDKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTRIARNGTLMTHFGRVNIRNAKYEYDFTPLDPMCDCYTCTNYTRA
YLRHLIKADETFGLRLCSYHNLHFLVNLMKDVRQAIMDDNLLEFREDFCERYGYNQPNAKDF
>Mature_381_residues
TEHAIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQATVKTMSPEELKTLGSGIILSNTYHLWLRPGDELVAEAG
GLHKFMNWDQPILTDSGGFQVYSLVQNKKNITEEGVKFKSHLDGRELFLNPEKAISIQNNLGSDIMMSFDECPPFYQPYD
YVKASVERTSRWAERGLNAHRRPNDQGLFGIVQGAGFEDLRRQSASDLTSMDFAGYSIGGLAVGESHKEMNAVLDFTTPM
LPEDKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTRIARNGTLMTHFGRVNIRNAKYEYDFTPLDPMCDCYTCTNYTRAY
LRHLIKADETFGLRLCSYHNLHFLVNLMKDVRQAIMDDNLLEFREDFCERYGYNQPNAKDF

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=360, Percent_Identity=46.3888888888889, Blast_Score=337, Evalue=8e-93,
Organism=Escherichia coli, GI1786607, Length=360, Percent_Identity=52.7777777777778, Blast_Score=384, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17544680, Length=355, Percent_Identity=45.0704225352113, Blast_Score=295, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI25146502, Length=209, Percent_Identity=30.1435406698565, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI19920510, Length=372, Percent_Identity=44.3548387096774, Blast_Score=321, Evalue=6e-88,
Organism=Drosophila melanogaster, GI21355311, Length=231, Percent_Identity=26.4069264069264, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 43371; Mature: 43240

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEHAIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQATVKTMSPEELKTLGSGI
CCCHHHHHHHHHHHHCCCHHHCCEECCCCCCCCCEEEECCCCCEEECCCHHHHHHHCCCE
ILSNTYHLWLRPGDELVAEAGGLHKFMNWDQPILTDSGGFQVYSLVQNKKNITEEGVKFK
EEECEEEEEECCCHHHHHHHCCHHHHHCCCCCEEECCCCCEEHHHHHHHHCHHHHHHHHH
SHLDGRELFLNPEKAISIQNNLGSDIMMSFDECPPFYQPYDYVKASVERTSRWAERGLNA
HCCCCCCEEECHHHHEEECCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
HRRPNDQGLFGIVQGAGFEDLRRQSASDLTSMDFAGYSIGGLAVGESHKEMNAVLDFTTP
CCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHHHHCCCC
MLPEDKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTRIARNGTLMTHFGRVNIRNAKYEY
CCCCCCCCEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEECEEEEECCEEEE
DFTPLDPMCDCYTCTNYTRAYLRHLIKADETFGLRLCSYHNLHFLVNLMKDVRQAIMDDN
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCH
LLEFREDFCERYGYNQPNAKDF
HHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TEHAIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQATVKTMSPEELKTLGSGI
CCHHHHHHHHHHHHCCCHHHCCEECCCCCCCCCEEEECCCCCEEECCCHHHHHHHCCCE
ILSNTYHLWLRPGDELVAEAGGLHKFMNWDQPILTDSGGFQVYSLVQNKKNITEEGVKFK
EEECEEEEEECCCHHHHHHHCCHHHHHCCCCCEEECCCCCEEHHHHHHHHCHHHHHHHHH
SHLDGRELFLNPEKAISIQNNLGSDIMMSFDECPPFYQPYDYVKASVERTSRWAERGLNA
HCCCCCCEEECHHHHEEECCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
HRRPNDQGLFGIVQGAGFEDLRRQSASDLTSMDFAGYSIGGLAVGESHKEMNAVLDFTTP
CCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHHHHCCCC
MLPEDKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTRIARNGTLMTHFGRVNIRNAKYEY
CCCCCCCCEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEECEEEEECCEEEE
DFTPLDPMCDCYTCTNYTRAYLRHLIKADETFGLRLCSYHNLHFLVNLMKDVRQAIMDDN
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCH
LLEFREDFCERYGYNQPNAKDF
HHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]