Click here to switch to the map view.

The map label for this gene is pmrB [H]

Identifier: 125623019

GI number: 125623019

Start: 136666

End: 137811

Strand: Reverse

Name: pmrB [H]

Synonym: llmg_0140

Alternate gene names: 125623019

Gene position: 137811-136666 (Counterclockwise)

Preceding gene: 125623032

Following gene: 125623009

Centisome position: 5.45

GC content: 40.05

Gene sequence:

>1146_bases
ATGACGGGAATTGGCTCAAGCCTTATTTCCCCTTTTATATCACTCTATATCGGAACACTAGGTCACTATTCAAAAACAGA
ATTAAATATTTGGTCTGGTCTGGTTTTCTCATCTACTTTTATTGTTTTAGCCATCGTTTCTCCCCTATGGGGAAGATTAG
CTGACCAAAAAGGTCGAAAGTTGATGCTCTTAAGAGCCTCTCTCGGAATGGCCATTTCAATTTCTCTAATGGCTTTCGTC
ACGGCTGCTTGGCAACTCCTTATTTTGAGAATGTTGCTTGGTGCTTTTTCTGGATTTGTATCCAATTCAATGGCTTTGAT
GGCAAGCTCGGCACCCAAAGAAAAATCCGGTTCAGTTTTATCATTACTCACAACAGGTTCTGTAGCTGGAACATTAATCG
GACCTATAGTTGGTGGGGTCCTCGTTAATATAACTGGATATCGTCGTGTCTTTAGCGTCACAGGTATCATCATGTTCCTT
GTTTTTTTCCTTGCACTTTTTTTGGTCAAAGAAACTTTTAAACCAGTTGAAAAGAAAAACATGCTCTCCTACAAACAAGT
ATGGGAAGTTGTCACACACCCTGCAATAATATGGGGAATGTTTTTCGCAACATTGATTACCCAAATGACCAATCAATCCA
TTAATCCTGTTCTTTCACTCTATGTACAAGAACTCATGCATGGTCAAGGGAATATCACTTTCATGGCTGGGCTTGTCGCC
GCCGCTCCAGGAATAGTAACCTTAGTTGCTGCCCCATTTTTAGGGAGACTTGGTGATAAAGTCGGTCAAAGAAAAATTCT
TGGCTTTGGACTTTTCTTCTCACTTATTGTCTTTTCAATTACCGCACTGACAACTAATGTTTGGTTTCTGATTGTTATGA
GGCTCTTTGTCGGAATTTCTGATGCTGCGATTTTACCATCTGTTCAAGCTATTCTGGCCAAAGAATCTCCTCAAGCTGTC
ACCGGCCGTATTTTCTCTTACAATCAATCCGCTCAATCAATCGGTGCCTTTGCTGGTCCTCTATTAGGCTCAGCTATTGC
TGGTTTTATCGACTATCGTTATGTATTTGTAGGTTCAGCAATCCTTGTCATCTTCAATCTGATCAACTACTTTACACACA
ATAATTCAGTTACACTAAAAAAGTAA

Upstream 100 bases:

>100_bases
AGTTTTATAATATTATTATGAATACATCAAGAAAGAAGTACATTTATTCATGAAAGAATCATGGCAACGAAATTTAATCG
TCCTTTGGTTTGGGACATTT

Downstream 100 bases:

>100_bases
TTGTATAAAAAGAGCTTCATTTTTGAAGCTCTTTTTTTGCTTGTTCAATTTGCTTCTTAATCGATTCAAAACCAGTTCCA
CCTAAAGAATTCCTTCGTGA

Product: multidrug resistance efflux pump

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MTGIGSSLISPFISLYIGTLGHYSKTELNIWSGLVFSSTFIVLAIVSPLWGRLADQKGRKLMLLRASLGMAISISLMAFV
TAAWQLLILRMLLGAFSGFVSNSMALMASSAPKEKSGSVLSLLTTGSVAGTLIGPIVGGVLVNITGYRRVFSVTGIIMFL
VFFLALFLVKETFKPVEKKNMLSYKQVWEVVTHPAIIWGMFFATLITQMTNQSINPVLSLYVQELMHGQGNITFMAGLVA
AAPGIVTLVAAPFLGRLGDKVGQRKILGFGLFFSLIVFSITALTTNVWFLIVMRLFVGISDAAILPSVQAILAKESPQAV
TGRIFSYNQSAQSIGAFAGPLLGSAIAGFIDYRYVFVGSAILVIFNLINYFTHNNSVTLKK

Sequences:

>Translated_381_residues
MTGIGSSLISPFISLYIGTLGHYSKTELNIWSGLVFSSTFIVLAIVSPLWGRLADQKGRKLMLLRASLGMAISISLMAFV
TAAWQLLILRMLLGAFSGFVSNSMALMASSAPKEKSGSVLSLLTTGSVAGTLIGPIVGGVLVNITGYRRVFSVTGIIMFL
VFFLALFLVKETFKPVEKKNMLSYKQVWEVVTHPAIIWGMFFATLITQMTNQSINPVLSLYVQELMHGQGNITFMAGLVA
AAPGIVTLVAAPFLGRLGDKVGQRKILGFGLFFSLIVFSITALTTNVWFLIVMRLFVGISDAAILPSVQAILAKESPQAV
TGRIFSYNQSAQSIGAFAGPLLGSAIAGFIDYRYVFVGSAILVIFNLINYFTHNNSVTLKK
>Mature_380_residues
TGIGSSLISPFISLYIGTLGHYSKTELNIWSGLVFSSTFIVLAIVSPLWGRLADQKGRKLMLLRASLGMAISISLMAFVT
AAWQLLILRMLLGAFSGFVSNSMALMASSAPKEKSGSVLSLLTTGSVAGTLIGPIVGGVLVNITGYRRVFSVTGIIMFLV
FFLALFLVKETFKPVEKKNMLSYKQVWEVVTHPAIIWGMFFATLITQMTNQSINPVLSLYVQELMHGQGNITFMAGLVAA
APGIVTLVAAPFLGRLGDKVGQRKILGFGLFFSLIVFSITALTTNVWFLIVMRLFVGISDAAILPSVQAILAKESPQAVT
GRIFSYNQSAQSIGAFAGPLLGSAIAGFIDYRYVFVGSAILVIFNLINYFTHNNSVTLKK

Specific function: Confers resistance to fosfomycin and deoxycholate [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. MdtG (TC 2.A.1.2.20) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787291, Length=368, Percent_Identity=45.1086956521739, Blast_Score=305, Evalue=2e-84,
Organism=Caenorhabditis elegans, GI25146993, Length=375, Percent_Identity=24, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI193210343, Length=376, Percent_Identity=24.2021276595745, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI161078541, Length=360, Percent_Identity=24.1666666666667, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001958 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 41094; Mature: 40962

Theoretical pI: Translated: 10.70; Mature: 10.70

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGIGSSLISPFISLYIGTLGHYSKTELNIWSGLVFSSTFIVLAIVSPLWGRLADQKGRK
CCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
LMLLRASLGMAISISLMAFVTAAWQLLILRMLLGAFSGFVSNSMALMASSAPKEKSGSVL
EEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCEE
SLLTTGSVAGTLIGPIVGGVLVNITGYRRVFSVTGIIMFLVFFLALFLVKETFKPVEKKN
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
MLSYKQVWEVVTHPAIIWGMFFATLITQMTNQSINPVLSLYVQELMHGQGNITFMAGLVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHH
AAPGIVTLVAAPFLGRLGDKVGQRKILGFGLFFSLIVFSITALTTNVWFLIVMRLFVGIS
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DAAILPSVQAILAKESPQAVTGRIFSYNQSAQSIGAFAGPLLGSAIAGFIDYRYVFVGSA
CHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILVIFNLINYFTHNNSVTLKK
HHHHHHHHHHHHCCCEEEECC
>Mature Secondary Structure 
TGIGSSLISPFISLYIGTLGHYSKTELNIWSGLVFSSTFIVLAIVSPLWGRLADQKGRK
CCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
LMLLRASLGMAISISLMAFVTAAWQLLILRMLLGAFSGFVSNSMALMASSAPKEKSGSVL
EEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCEE
SLLTTGSVAGTLIGPIVGGVLVNITGYRRVFSVTGIIMFLVFFLALFLVKETFKPVEKKN
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
MLSYKQVWEVVTHPAIIWGMFFATLITQMTNQSINPVLSLYVQELMHGQGNITFMAGLVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHH
AAPGIVTLVAAPFLGRLGDKVGQRKILGFGLFFSLIVFSITALTTNVWFLIVMRLFVGIS
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DAAILPSVQAILAKESPQAVTGRIFSYNQSAQSIGAFAGPLLGSAIAGFIDYRYVFVGSA
CHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILVIFNLINYFTHNNSVTLKK
HHHHHHHHHHHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA