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The map label for this gene is pdhD [H]

Identifier: 125622950

GI number: 125622950

Start: 65273

End: 66691

Strand: Reverse

Name: pdhD [H]

Synonym: llmg_0071

Alternate gene names: 125622950

Gene position: 66691-65273 (Counterclockwise)

Preceding gene: 125622951

Following gene: 125622944

Centisome position: 2.64

GC content: 40.17

Gene sequence:

>1419_bases
ATGGTTGTTGGTGCACAAGCAACAGAAGTTGATTTGGTTGTTATTGGTTCAGGCCCTGGCGGTTATGTTGCGGCCATCCG
TGCGGCTGAACTTGGTAAAAAAGTTACGATTATTGAAAAAGATAATGTCGGTGGGGTTTGTTTAAATATTGGATGTATCC
CATCAAAAGCATTGATTAATATTGGTCATCATTATCAAGAATCTTTGGAAGAAGAAAAGGGTGAAAATCCTTTTGGACTT
TCTGTCGGAAATGTTAAATTAAATTGGGAATCTGCCCAAAAATGGAAACAAGATAAAGTTGTCAACCAGTTGACAGGTGG
CGTTAAAATGCTACTTAAAAAACACAAAGTTGATGTAATTCAAGGAACAGCTGAGTTTATTGATAACAATACAATCAATG
TTGAACAAGAAGATGGTTTCCAACTTTTGCAGTTCAACGATGTAATTATCTCAACTGGTTCACGTCCTATTGAAATCCCA
TCTTTCCCATTTGGTGGTCGTATTATTGACTCAACAGGTGCTTTGTCACTTCCAGAAGTGCCTAAACATTTGATTATTGT
TGGGGGTGGCGTTATTGGTTCTGAGCTTGGTGGAGCTTACCGCATGCTCGGTTCTAAGATTACAATTGTTGAAGGCTTGG
ACCACATTTTAAACGGTTTTGATAAAGAAATGTCTGATATCATTGCGAATCGTGTGAAATCTGCTGGTTCTGAAATCTTT
ACATCGGCGATGGCTAAATCAGCTACTCAAACCGATAAAGATGTGACTTTAACTTTTGAAGTTGACGGAAAAGAACAAAC
AGTAACTGGTGATTACTTACTCGTTTCTGTTGGACGTCGTCCAAATACTGATTTGATTGGCTTGAACAATACTGATGTCA
AATTGACTGACCGTGGTTTGATTGAAGTTGATGATTCTTATGCAACGAATGTTCCTCATATTTACGCGATTGGTGATGTG
GTTCCTGGACCAATGCTCGCACACAAAGCTTCTTTCCAAGCTAAAGTTGCTGCAGCGGCCATTGCTGGAGCTGAAGACGA
CGTGGACTTGCACGTTGCTTTGCCAGCCGTTGCTTATACAACAACTGAACTAGCAACAGTTGGAGAAACGCCTGAATCAG
TTAAAGACCGTAAAGATGTCAAGATCTCTAAGTTCCCATTTGCTGCAAATGGTCGTGCCATTTCAATGAATAATACCACT
GGTTTCTTACGTTTGATTACCGAAACTAAAGAAGGTGCCTTAATTGGTGCTCAAATCGTTGGTCCTGGCGCATCTGACCT
GATCTCTGGTTTATCACTAGCGATTGAAAATGGTTTGACTTCTAAAGATATCTCATTGACTATCCAACCTCACCCAACAC
TTGGTGAAGCGATTATGGATACAGCTGAATTGGCTGATGGCTTGCCAATTCACGTTTAA

Upstream 100 bases:

>100_bases
ATAGTTTTTAAATAAAATTCTTAGGACAAGTCTGTCAGTAAGTTTACAACGTTGTAAACCCTAGTTGACATTGAAGTTTA
CAAGAAAAGAGGAATAAAAA

Downstream 100 bases:

>100_bases
AATAAGAAAAATCACTGACGAAATAATCTGTCAGTGATTTTTTTTGAATAAATTTATAAAAGTAGGACTAATTAAAGCAA
TAAAACAGATGAAAGCAATA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MVVGAQATEVDLVVIGSGPGGYVAAIRAAELGKKVTIIEKDNVGGVCLNIGCIPSKALINIGHHYQESLEEEKGENPFGL
SVGNVKLNWESAQKWKQDKVVNQLTGGVKMLLKKHKVDVIQGTAEFIDNNTINVEQEDGFQLLQFNDVIISTGSRPIEIP
SFPFGGRIIDSTGALSLPEVPKHLIIVGGGVIGSELGGAYRMLGSKITIVEGLDHILNGFDKEMSDIIANRVKSAGSEIF
TSAMAKSATQTDKDVTLTFEVDGKEQTVTGDYLLVSVGRRPNTDLIGLNNTDVKLTDRGLIEVDDSYATNVPHIYAIGDV
VPGPMLAHKASFQAKVAAAAIAGAEDDVDLHVALPAVAYTTTELATVGETPESVKDRKDVKISKFPFAANGRAISMNNTT
GFLRLITETKEGALIGAQIVGPGASDLISGLSLAIENGLTSKDISLTIQPHPTLGEAIMDTAELADGLPIHV

Sequences:

>Translated_472_residues
MVVGAQATEVDLVVIGSGPGGYVAAIRAAELGKKVTIIEKDNVGGVCLNIGCIPSKALINIGHHYQESLEEEKGENPFGL
SVGNVKLNWESAQKWKQDKVVNQLTGGVKMLLKKHKVDVIQGTAEFIDNNTINVEQEDGFQLLQFNDVIISTGSRPIEIP
SFPFGGRIIDSTGALSLPEVPKHLIIVGGGVIGSELGGAYRMLGSKITIVEGLDHILNGFDKEMSDIIANRVKSAGSEIF
TSAMAKSATQTDKDVTLTFEVDGKEQTVTGDYLLVSVGRRPNTDLIGLNNTDVKLTDRGLIEVDDSYATNVPHIYAIGDV
VPGPMLAHKASFQAKVAAAAIAGAEDDVDLHVALPAVAYTTTELATVGETPESVKDRKDVKISKFPFAANGRAISMNNTT
GFLRLITETKEGALIGAQIVGPGASDLISGLSLAIENGLTSKDISLTIQPHPTLGEAIMDTAELADGLPIHV
>Mature_472_residues
MVVGAQATEVDLVVIGSGPGGYVAAIRAAELGKKVTIIEKDNVGGVCLNIGCIPSKALINIGHHYQESLEEEKGENPFGL
SVGNVKLNWESAQKWKQDKVVNQLTGGVKMLLKKHKVDVIQGTAEFIDNNTINVEQEDGFQLLQFNDVIISTGSRPIEIP
SFPFGGRIIDSTGALSLPEVPKHLIIVGGGVIGSELGGAYRMLGSKITIVEGLDHILNGFDKEMSDIIANRVKSAGSEIF
TSAMAKSATQTDKDVTLTFEVDGKEQTVTGDYLLVSVGRRPNTDLIGLNNTDVKLTDRGLIEVDDSYATNVPHIYAIGDV
VPGPMLAHKASFQAKVAAAAIAGAEDDVDLHVALPAVAYTTTELATVGETPESVKDRKDVKISKFPFAANGRAISMNNTT
GFLRLITETKEGALIGAQIVGPGASDLISGLSLAIENGLTSKDISLTIQPHPTLGEAIMDTAELADGLPIHV

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=463, Percent_Identity=38.6609071274298, Blast_Score=308, Evalue=7e-84,
Organism=Homo sapiens, GI50301238, Length=464, Percent_Identity=28.8793103448276, Blast_Score=178, Evalue=1e-44,
Organism=Homo sapiens, GI291045266, Length=463, Percent_Identity=27.4298056155508, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI22035672, Length=472, Percent_Identity=27.7542372881356, Blast_Score=142, Evalue=9e-34,
Organism=Homo sapiens, GI291045268, Length=458, Percent_Identity=26.6375545851528, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI33519430, Length=456, Percent_Identity=25.4385964912281, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI33519428, Length=456, Percent_Identity=25.4385964912281, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI33519426, Length=456, Percent_Identity=25.4385964912281, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI148277065, Length=456, Percent_Identity=25.4385964912281, Blast_Score=133, Evalue=5e-31,
Organism=Homo sapiens, GI148277071, Length=456, Percent_Identity=25.4385964912281, Blast_Score=132, Evalue=8e-31,
Organism=Escherichia coli, GI1786307, Length=466, Percent_Identity=40.5579399141631, Blast_Score=325, Evalue=4e-90,
Organism=Escherichia coli, GI87082354, Length=461, Percent_Identity=31.0195227765727, Blast_Score=191, Evalue=7e-50,
Organism=Escherichia coli, GI1789915, Length=441, Percent_Identity=29.7052154195011, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI87081717, Length=459, Percent_Identity=28.322440087146, Blast_Score=169, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI32565766, Length=464, Percent_Identity=39.8706896551724, Blast_Score=323, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI17557007, Length=481, Percent_Identity=26.4033264033264, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI71983429, Length=447, Percent_Identity=25.9507829977629, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71983419, Length=447, Percent_Identity=25.9507829977629, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71982272, Length=481, Percent_Identity=27.027027027027, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17559934, Length=225, Percent_Identity=26.6666666666667, Blast_Score=65, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6321091, Length=477, Percent_Identity=38.1551362683438, Blast_Score=290, Evalue=4e-79,
Organism=Saccharomyces cerevisiae, GI6325240, Length=481, Percent_Identity=30.3534303534304, Blast_Score=189, Evalue=7e-49,
Organism=Saccharomyces cerevisiae, GI6325166, Length=468, Percent_Identity=27.7777777777778, Blast_Score=159, Evalue=6e-40,
Organism=Drosophila melanogaster, GI21358499, Length=473, Percent_Identity=39.7463002114165, Blast_Score=317, Evalue=1e-86,
Organism=Drosophila melanogaster, GI17737741, Length=478, Percent_Identity=26.1506276150628, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24640549, Length=482, Percent_Identity=27.1784232365145, Blast_Score=135, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24640553, Length=497, Percent_Identity=27.1629778672032, Blast_Score=135, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24640551, Length=480, Percent_Identity=27.2916666666667, Blast_Score=134, Evalue=1e-31,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49866; Mature: 49866

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVGAQATEVDLVVIGSGPGGYVAAIRAAELGKKVTIIEKDNVGGVCLNIGCIPSKALIN
CEECCCCCEEEEEEEECCCCCEEEEEEHHHCCCEEEEEEECCCCEEEEEECCCCCHHHHH
IGHHYQESLEEEKGENPFGLSVGNVKLNWESAQKWKQDKVVNQLTGGVKMLLKKHKVDVI
CCHHHHHHHHHHCCCCCCCEEECCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCHHEE
QGTAEFIDNNTINVEQEDGFQLLQFNDVIISTGSRPIEIPSFPFGGRIIDSTGALSLPEV
ECHHHHHCCCEEEEECCCCEEEEEECEEEEECCCCCEECCCCCCCCEEECCCCCCCCCCC
PKHLIIVGGGVIGSELGGAYRMLGSKITIVEGLDHILNGFDKEMSDIIANRVKSAGSEIF
CCEEEEEECCEECHHCCHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TSAMAKSATQTDKDVTLTFEVDGKEQTVTGDYLLVSVGRRPNTDLIGLNNTDVKLTDRGL
HHHHHHHCCCCCCCEEEEEEECCCCEEEECCEEEEEECCCCCCCEEECCCCCEEEECCCE
IEVDDSYATNVPHIYAIGDVVPGPMLAHKASFQAKVAAAAIAGAEDDVDLHVALPAVAYT
EEECCCCCCCCCEEEEECCCCCCCHHHHCCCCHHHHHHHHHCCCCCCCEEEEEECEEEEE
TTELATVGETPESVKDRKDVKISKFPFAANGRAISMNNTTGFLRLITETKEGALIGAQIV
HHHHEECCCCCHHHHCCCCCEEEECCCCCCCEEEEECCCCEEEEEEEECCCCEEEEEEEE
GPGASDLISGLSLAIENGLTSKDISLTIQPHPTLGEAIMDTAELADGLPIHV
CCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MVVGAQATEVDLVVIGSGPGGYVAAIRAAELGKKVTIIEKDNVGGVCLNIGCIPSKALIN
CEECCCCCEEEEEEEECCCCCEEEEEEHHHCCCEEEEEEECCCCEEEEEECCCCCHHHHH
IGHHYQESLEEEKGENPFGLSVGNVKLNWESAQKWKQDKVVNQLTGGVKMLLKKHKVDVI
CCHHHHHHHHHHCCCCCCCEEECCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCHHEE
QGTAEFIDNNTINVEQEDGFQLLQFNDVIISTGSRPIEIPSFPFGGRIIDSTGALSLPEV
ECHHHHHCCCEEEEECCCCEEEEEECEEEEECCCCCEECCCCCCCCEEECCCCCCCCCCC
PKHLIIVGGGVIGSELGGAYRMLGSKITIVEGLDHILNGFDKEMSDIIANRVKSAGSEIF
CCEEEEEECCEECHHCCHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TSAMAKSATQTDKDVTLTFEVDGKEQTVTGDYLLVSVGRRPNTDLIGLNNTDVKLTDRGL
HHHHHHHCCCCCCCEEEEEEECCCCEEEECCEEEEEECCCCCCCEEECCCCCEEEECCCE
IEVDDSYATNVPHIYAIGDVVPGPMLAHKASFQAKVAAAAIAGAEDDVDLHVALPAVAYT
EEECCCCCCCCCEEEEECCCCCCCHHHHCCCCHHHHHHHHHCCCCCCCEEEEEECEEEEE
TTELATVGETPESVKDRKDVKISKFPFAANGRAISMNNTTGFLRLITETKEGALIGAQIV
HHHHEECCCCCHHHHCCCCCEEEECCCCCCCEEEEECCCCEEEEEEEECCCCEEEEEEEE
GPGASDLISGLSLAIENGLTSKDISLTIQPHPTLGEAIMDTAELADGLPIHV
CCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2200674; 2253629; 6896188; 8805537 [H]