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The map label for this gene is dnaN [H]

Identifier: 125622884

GI number: 125622884

Start: 1522

End: 2664

Strand: Direct

Name: dnaN [H]

Synonym: llmg_0002

Alternate gene names: 125622884

Gene position: 1522-2664 (Clockwise)

Preceding gene: 125622883

Following gene: 125622885

Centisome position: 0.06

GC content: 32.2

Gene sequence:

>1143_bases
ATGATTAAATTTTCAATTAATAAAACTGCCTTTCAAAATGCTTTGAAAATTACCAAACAAGCAATTGGTTCTAAAGTTAC
AATTCCTGCTTTAACAAAGTTAAAAATTGAAGTTGAAGAAAAAGGAATTACCTTAATTGGTTCAAATGGTCAAATTTCAA
TCAAAAACTTTTTGCCAGCTGATAATAAAGATGCAAGTATGCTTATTTCAGGAACTGGTTCTGTCTTATTAGAAGCTGCT
TTCTTTGAAAATGTAGTAAGTCAACTTCCTGAAGTTACTTTGGAATTTATCGAAAAAGAACAAAAACAAGTTTTGTTGAC
TTCTGGTAAATCAGAAATTACTTTAAAAGGTTTAGATTCTGAAATTTATCCACATTTACAAGAAATCTCTGAAGGTTCTT
CTTTAAAAATGAAAGTGAAAGTTTTGAAAGAAATTTTCACAGAGACTGTTTTTGCTGTAAGTACTCAAGAAAATCGTCCA
ATTTTCACAGGGGTTCACTTAGAAACTTTGTCAACGGGTGAATTAAAAGCAGTAGCAACTGACTCACATCGAATGAGCCA
ACGTTTATTGCCTTTAGAAGATACAGACTTAAAATTTGATGTAATTTTACCAAGTAAATCAATCAATAGTTTTAAAAATG
TTTTTACAAATGATGAAGAAGAGATTGAAATTTTCATCAGTGGAAGCCAAATGCTTTTCCAAAATGAAACAATTAGTTAT
TATAGTCGTTTGATTGAGGGTTCTTATCCTGATACTAATCGTTTGATTCCAAATGAAGCTGATTATACTTTAGATTTGGT
TTTTGATGCTGCGCAATTGCGTCATACAATGGATCGTGCACGTTTATTGACAGTAATGACAACCAACGGAACAGTAAAAT
TGACTGTTTCAGGTGATTCTGTCGTAACCACAGCAAACTCTCCTGAAGTAGGTTCTGTTCATGAAGAACTGACAGCCTTA
TCAAAAGAAGGTAATGATTTATCTATCAGTTTCAATCCTGAGTATTTGATTGATGCTTTGAAAGTGATTAAAGCACCAGA
AGTACGAATTCGTTTCATTTCTAACGTTCGTCCGTTCACTTTGCAACCACGGAATGAAGAATCTGGATTTGTTCAATTGA
TTACACCAGTACGTACAAATTAA

Upstream 100 bases:

>100_bases
AAAGCCTTACGCCTCTAAGAGGGTTTGTCTTATACAGATTATCCACAGGACCTACTACTATTACTATTATATTTATAAAA
AGAAATTATAAAGGAATATC

Downstream 100 bases:

>100_bases
TTTTACTGACAGCTCTTTTGTCAGCTTAGATTTACTGACGAAAATGTCTGTCAGTAAATTTAGATTTTGTTATAATGGAC
TTAACGAAAGTTAGGTCTAT

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MIKFSINKTAFQNALKITKQAIGSKVTIPALTKLKIEVEEKGITLIGSNGQISIKNFLPADNKDASMLISGTGSVLLEAA
FFENVVSQLPEVTLEFIEKEQKQVLLTSGKSEITLKGLDSEIYPHLQEISEGSSLKMKVKVLKEIFTETVFAVSTQENRP
IFTGVHLETLSTGELKAVATDSHRMSQRLLPLEDTDLKFDVILPSKSINSFKNVFTNDEEEIEIFISGSQMLFQNETISY
YSRLIEGSYPDTNRLIPNEADYTLDLVFDAAQLRHTMDRARLLTVMTTNGTVKLTVSGDSVVTTANSPEVGSVHEELTAL
SKEGNDLSISFNPEYLIDALKVIKAPEVRIRFISNVRPFTLQPRNEESGFVQLITPVRTN

Sequences:

>Translated_380_residues
MIKFSINKTAFQNALKITKQAIGSKVTIPALTKLKIEVEEKGITLIGSNGQISIKNFLPADNKDASMLISGTGSVLLEAA
FFENVVSQLPEVTLEFIEKEQKQVLLTSGKSEITLKGLDSEIYPHLQEISEGSSLKMKVKVLKEIFTETVFAVSTQENRP
IFTGVHLETLSTGELKAVATDSHRMSQRLLPLEDTDLKFDVILPSKSINSFKNVFTNDEEEIEIFISGSQMLFQNETISY
YSRLIEGSYPDTNRLIPNEADYTLDLVFDAAQLRHTMDRARLLTVMTTNGTVKLTVSGDSVVTTANSPEVGSVHEELTAL
SKEGNDLSISFNPEYLIDALKVIKAPEVRIRFISNVRPFTLQPRNEESGFVQLITPVRTN
>Mature_380_residues
MIKFSINKTAFQNALKITKQAIGSKVTIPALTKLKIEVEEKGITLIGSNGQISIKNFLPADNKDASMLISGTGSVLLEAA
FFENVVSQLPEVTLEFIEKEQKQVLLTSGKSEITLKGLDSEIYPHLQEISEGSSLKMKVKVLKEIFTETVFAVSTQENRP
IFTGVHLETLSTGELKAVATDSHRMSQRLLPLEDTDLKFDVILPSKSINSFKNVFTNDEEEIEIFISGSQMLFQNETISY
YSRLIEGSYPDTNRLIPNEADYTLDLVFDAAQLRHTMDRARLLTVMTTNGTVKLTVSGDSVVTTANSPEVGSVHEELTAL
SKEGNDLSISFNPEYLIDALKVIKAPEVRIRFISNVRPFTLQPRNEESGFVQLITPVRTN

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=377, Percent_Identity=23.6074270557029, Blast_Score=117, Evalue=1e-27,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 42210; Mature: 42210

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKFSINKTAFQNALKITKQAIGSKVTIPALTKLKIEVEEKGITLIGSNGQISIKNFLPA
CEEEECCHHHHHHHHHHHHHHHCCEEEECEEEEEEEEEECCCEEEEECCCEEEEEECCCC
DNKDASMLISGTGSVLLEAAFFENVVSQLPEVTLEFIEKEQKQVLLTSGKSEITLKGLDS
CCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEECCCC
EIYPHLQEISEGSSLKMKVKVLKEIFTETVFAVSTQENRPIFTGVHLETLSTGELKAVAT
HHHHHHHHHCCCCCEEEHHHHHHHHHHHHEEEEECCCCCCEEEEEEEEEECCCCEEEEEC
DSHRMSQRLLPLEDTDLKFDVILPSKSINSFKNVFTNDEEEIEIFISGSQMLFQNETISY
CCHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHHCCCCCEEEEEEECCEEEEECCHHHH
YSRLIEGSYPDTNRLIPNEADYTLDLVFDAAQLRHTMDRARLLTVMTTNGTVKLTVSGDS
HHHHHCCCCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHEEEEEEEECCCEEEEEECCCE
VVTTANSPEVGSVHEELTALSKEGNDLSISFNPEYLIDALKVIKAPEVRIRFISNVRPFT
EEEECCCCCCCHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHCCCCEEEEEECCCCCEE
LQPRNEESGFVQLITPVRTN
ECCCCCCCCEEEEECCCCCC
>Mature Secondary Structure
MIKFSINKTAFQNALKITKQAIGSKVTIPALTKLKIEVEEKGITLIGSNGQISIKNFLPA
CEEEECCHHHHHHHHHHHHHHHCCEEEECEEEEEEEEEECCCEEEEECCCEEEEEECCCC
DNKDASMLISGTGSVLLEAAFFENVVSQLPEVTLEFIEKEQKQVLLTSGKSEITLKGLDS
CCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEECCCC
EIYPHLQEISEGSSLKMKVKVLKEIFTETVFAVSTQENRPIFTGVHLETLSTGELKAVAT
HHHHHHHHHCCCCCEEEHHHHHHHHHHHHEEEEECCCCCCEEEEEEEEEECCCCEEEEEC
DSHRMSQRLLPLEDTDLKFDVILPSKSINSFKNVFTNDEEEIEIFISGSQMLFQNETISY
CCHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHHCCCCCEEEEEEECCEEEEECCHHHH
YSRLIEGSYPDTNRLIPNEADYTLDLVFDAAQLRHTMDRARLLTVMTTNGTVKLTVSGDS
HHHHHCCCCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHEEEEEEEECCCEEEEEECCCE
VVTTANSPEVGSVHEELTALSKEGNDLSISFNPEYLIDALKVIKAPEVRIRFISNVRPFT
EEEECCCCCCCHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHCCCCEEEEEECCCCCEE
LQPRNEESGFVQLITPVRTN
ECCCCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]