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The map label for this gene is tfb

Identifier: 124486326

GI number: 124486326

Start: 1546921

End: 1547943

Strand: Reverse

Name: tfb

Synonym: Mlab_1511

Alternate gene names: 124486326

Gene position: 1547943-1546921 (Counterclockwise)

Preceding gene: 124486327

Following gene: 124486324

Centisome position: 85.76

GC content: 51.81

Gene sequence:

>1023_bases
ATGGAAACTGAAATTGAAAAACTGAAACAGCTACGAGCAGATAGAGAAAAAATGAAACAGCGTCAGGAAACGACGCTTGT
TACGAGGGAAAAGGAGAAGCAGGGTGGAAACGATGCGACGGCGACGGTCTGTCCGGAGTGCGGGAGCAAGCAGCTCGTTC
ATGATTATGAGCGTGCAGAGCTTGTCTGTCAGCACTGCGGTCTTGTTTTAGATGACGATTTCATTGACCGCGGGCCGGAA
TGGCGTGCATTTGATCATGATCAGCGGATGAAGAGATCGCGTGTCGGCGCACCAATGACCTTTACGATCCATGATAAAGG
TCTTTCCACCATGATCGACTGGAGAAACCGTGATAGTTACGGGCGTGCGATCTCGTCGAAGAATCGGGCTCAGTTATATC
GTCTGAGAAAATGGCAGCGTCGTATCAGAGTTTCCAATGCGACAGAGCGTAACCTGGCATTCGCACTTTCTGAACTTGAT
CGAATGGCCTCCGCTCTTGGTCTTCCGCGAAATGTTCGCGAGACCGCAGCAGTCGTTTACCGTGACGCCGTCGATAAGAA
TCTGATTCGCGGAAGAAGTATTGAGGGCGTTGCAGCCGCTGCGTTGTATGCTGCCTGCCGTCAGTGTAATGTTCCAAGAA
CGCTCGACGAGATCGCTGAAGTGTCCCGTGTTTCCAGAAAAGAGATCGGCAGGACTTACCGGTTCATCTCCCGTGAACTT
GGATTGAAACTTCTCCCGACTTCCCCTGGCGACTATGTTCCAAGGTTCTGCTCAGGTCTCGGTCTGAAAGGTGAGGTCCA
GTCTCGTGCAATGGAGATTTTAAAACAGGCTGGCGAGCGTGAACTTACGAGCGGACGGGGTCCGACCGGCGTTGCAGCCG
CTGCGATCTACATCTCCTCGATTCTTTCAGGAGAACGCAGGACCCAGCGCGAAGTTGCCGATGTGGCCGGCGTAACGGAA
GTTACGATCCGTAACAGATACAAGGAACTGGCTGAACAACTGGATATCGAGATTATCTTATAA

Upstream 100 bases:

>100_bases
AACCAGGCCTGAAAAACCCAGAAAAACTTCACCTCACAAATATGGCCGCGATATGCGCGCAAATCGGCGTTAATCCGTAG
ATTAAAAGGATCTTATAATC

Downstream 100 bases:

>100_bases
TCTCCTGAATATTTTTTCTCACCGAACTTTACAGGTGACGATACCATAGAGTTAATATGGATTTTATCCTATAGATTAGA
GATATTTCCGGGCTCGTATA

Product: transcription initiation factor IIB

Products: NA

Alternate protein names: TFIIB [H]

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
METEIEKLKQLRADREKMKQRQETTLVTREKEKQGGNDATATVCPECGSKQLVHDYERAELVCQHCGLVLDDDFIDRGPE
WRAFDHDQRMKRSRVGAPMTFTIHDKGLSTMIDWRNRDSYGRAISSKNRAQLYRLRKWQRRIRVSNATERNLAFALSELD
RMASALGLPRNVRETAAVVYRDAVDKNLIRGRSIEGVAAAALYAACRQCNVPRTLDEIAEVSRVSRKEIGRTYRFISREL
GLKLLPTSPGDYVPRFCSGLGLKGEVQSRAMEILKQAGERELTSGRGPTGVAAAAIYISSILSGERRTQREVADVAGVTE
VTIRNRYKELAEQLDIEIIL

Sequences:

>Translated_340_residues
METEIEKLKQLRADREKMKQRQETTLVTREKEKQGGNDATATVCPECGSKQLVHDYERAELVCQHCGLVLDDDFIDRGPE
WRAFDHDQRMKRSRVGAPMTFTIHDKGLSTMIDWRNRDSYGRAISSKNRAQLYRLRKWQRRIRVSNATERNLAFALSELD
RMASALGLPRNVRETAAVVYRDAVDKNLIRGRSIEGVAAAALYAACRQCNVPRTLDEIAEVSRVSRKEIGRTYRFISREL
GLKLLPTSPGDYVPRFCSGLGLKGEVQSRAMEILKQAGERELTSGRGPTGVAAAAIYISSILSGERRTQREVADVAGVTE
VTIRNRYKELAEQLDIEIIL
>Mature_340_residues
METEIEKLKQLRADREKMKQRQETTLVTREKEKQGGNDATATVCPECGSKQLVHDYERAELVCQHCGLVLDDDFIDRGPE
WRAFDHDQRMKRSRVGAPMTFTIHDKGLSTMIDWRNRDSYGRAISSKNRAQLYRLRKWQRRIRVSNATERNLAFALSELD
RMASALGLPRNVRETAAVVYRDAVDKNLIRGRSIEGVAAAALYAACRQCNVPRTLDEIAEVSRVSRKEIGRTYRFISREL
GLKLLPTSPGDYVPRFCSGLGLKGEVQSRAMEILKQAGERELTSGRGPTGVAAAAIYISSILSGERRTQREVADVAGVTE
VTIRNRYKELAEQLDIEIIL

Specific function: Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre- initiation complex (DNA-TBP-TFIIB) [H]

COG id: COG1405

COG function: function code K; Transcription initiation factor TFIIIB, Brf1 subunit/Transcription initiation factor TFIIB

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TFIIB-type zinc finger [H]

Homologues:

Organism=Homo sapiens, GI4504193, Length=286, Percent_Identity=39.5104895104895, Blast_Score=206, Evalue=2e-53,
Organism=Homo sapiens, GI22035556, Length=294, Percent_Identity=27.2108843537415, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17564892, Length=288, Percent_Identity=38.5416666666667, Blast_Score=201, Evalue=4e-52,
Organism=Caenorhabditis elegans, GI17531397, Length=293, Percent_Identity=24.5733788395904, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6325343, Length=319, Percent_Identity=29.4670846394984, Blast_Score=129, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6321685, Length=294, Percent_Identity=24.1496598639456, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI17136758, Length=278, Percent_Identity=37.7697841726619, Blast_Score=188, Evalue=4e-48,
Organism=Drosophila melanogaster, GI281361961, Length=300, Percent_Identity=27, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24647658, Length=300, Percent_Identity=27, Blast_Score=96, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006670
- InterPro:   IPR011028
- InterPro:   IPR013763
- InterPro:   IPR000812
- InterPro:   IPR013150
- InterPro:   IPR013137 [H]

Pfam domain/function: PF00382 TFIIB; PF08271 TFIIB_Zn_Ribbon [H]

EC number: NA

Molecular weight: Translated: 38457; Mature: 38457

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: PS51134 ZF_TFIIB ; PS00782 TFIIB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METEIEKLKQLRADREKMKQRQETTLVTREKEKQGGNDATATVCPECGSKQLVHDYERAE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHH
LVCQHCGLVLDDDFIDRGPEWRAFDHDQRMKRSRVGAPMTFTIHDKGLSTMIDWRNRDSY
HHHHHCCCEECCHHHCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCHHHHHHCCCCCHH
GRAISSKNRAQLYRLRKWQRRIRVSNATERNLAFALSELDRMASALGLPRNVRETAAVVY
HHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
RDAVDKNLIRGRSIEGVAAAALYAACRQCNVPRTLDEIAEVSRVSRKEIGRTYRFISREL
HHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLKLLPTSPGDYVPRFCSGLGLKGEVQSRAMEILKQAGERELTSGRGPTGVAAAAIYISS
CCEEECCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHH
ILSGERRTQREVADVAGVTEVTIRNRYKELAEQLDIEIIL
HHCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCEEEC
>Mature Secondary Structure
METEIEKLKQLRADREKMKQRQETTLVTREKEKQGGNDATATVCPECGSKQLVHDYERAE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHH
LVCQHCGLVLDDDFIDRGPEWRAFDHDQRMKRSRVGAPMTFTIHDKGLSTMIDWRNRDSY
HHHHHCCCEECCHHHCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCHHHHHHCCCCCHH
GRAISSKNRAQLYRLRKWQRRIRVSNATERNLAFALSELDRMASALGLPRNVRETAAVVY
HHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
RDAVDKNLIRGRSIEGVAAAALYAACRQCNVPRTLDEIAEVSRVSRKEIGRTYRFISREL
HHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLKLLPTSPGDYVPRFCSGLGLKGEVQSRAMEILKQAGERELTSGRGPTGVAAAAIYISS
CCEEECCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHH
ILSGERRTQREVADVAGVTEVTIRNRYKELAEQLDIEIIL
HHCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA