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The map label for this gene is prf1 [H]

Identifier: 124486324

GI number: 124486324

Start: 1543621

End: 1544907

Strand: Reverse

Name: prf1 [H]

Synonym: Mlab_1509

Alternate gene names: 124486324

Gene position: 1544907-1543621 (Counterclockwise)

Preceding gene: 124486326

Following gene: 124486323

Centisome position: 85.59

GC content: 50.43

Gene sequence:

>1287_bases
ATGGCAGAGGAACATTCCCAGCACGAAATCGAAAAGGATGAAGCAAGAAAGCGATATGAGTTCAAAAAATCACTGGAACG
ACTGGAGGAGAAAGAGGGGTCGGGAACAGAACTGATCTCTCTCTATATCCCCCCGGATAAGCAGATATATGATGTAACTG
CCCAGCTTCGCGATGAATATGGTCAGTGCGCGAACATCAAAAGCAAACAGACACGGTCAAATGTTCAGAGCGCTCTCTCT
TCAATTCTTGCACGCCTGAAATACTTCAAAACACCCCCGGAAAACGGCATGGCGATTTTCTGCGGAGCAATCAACGTGGG
CGGCGACAAAACCGATCTGGAGTCCATCATCGTCGAACCTCCGGAGGTCATCCAGACTTACAGTTACCGGTGCAGTTCTA
CCTTCGAACTTGAACCTCTTAAGGCGATGCTCGATGACAAATATGTATATGGGCTTTTAGTCATCGACCGTCGCGAAGCC
TACTGGGGTTTCCTTCGAGGAACGCACATTGAGCCGATCAACGGAACCAACTCCACCGTTCCCGGAAAACAAAGGAAAGG
TGGTCAGTCCAGTGTCCGTTTCGAACGCCTCAGACTGATTGCGATCCATGATTTCTACAGCAAAGTCGGCGAACGTGCTT
CGGCGGCGTTCCTTGCCGAGCCCAACTTCTTTGAAAAGTTCAAGGGAGTTTTGATCGGCGGTCCGATGCCGACCAAAGAG
GAGTTCGCCTCCGGCGATTTCCTTCACCACGAAGTCAAGAAACGGATCATCGGTCTCTTTGATGCATCCTACACCAATGA
ATTCGGTCTGCGCGAACTGGTGGACAATGCCCAGGATGCTCTTCGCGGTGTCGGAATAATGGACGAAAAAGCAGAAATGT
CCAGATTCTTCAAAGAACTCATCAAAGAGAACGGGGCGGCTGCTTACGGAGAAGACAGTGTTCGGCTCAATCTGGAGGCA
GGTGCGGTCGACACGCTCCTCCTCTCAGAAAAACTGAGAAAGGCACGGCTCACCATCAAATGCGGGAACTGTGACTACGA
AGAAATCAAAACCATGCAGATCGAAGCTGGGAAAAAGTTCAAAGATCTGCCGTTCGGCCGCTGTCCGAAGTGTCAGTCCT
CACTCTTCCTTGAAAAGGAAGCCGATATCATCGATGAACTGACCGAGCTTGCCGATGCGTCAAACACCAGAGTAGAGATC
ATTTCAGAAGATTTCGAGGAAGGAGCCATGCTTTACTCCGCATTCGGCGGGATTGCAGCCGTCCTTCGATATCCAACGGG
GATGTAA

Upstream 100 bases:

>100_bases
TCGCGATTTCCTGACGCGATGTTTAATCACCTTGCACATCTAATAATACACCACATTCTACCGCCGGATTAATACCGGAT
ATTAAAGGGTTGTATATACA

Downstream 100 bases:

>100_bases
CATGTATCGAGAATATATTCAGAAAACAATCGATCTGCTTCGCGAAGCAACAGGTCTTGAAGATGTCATGATCACCGACG
GAGGAGATCATGCGGATCTT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: Translation termination factor aRF1 [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MAEEHSQHEIEKDEARKRYEFKKSLERLEEKEGSGTELISLYIPPDKQIYDVTAQLRDEYGQCANIKSKQTRSNVQSALS
SILARLKYFKTPPENGMAIFCGAINVGGDKTDLESIIVEPPEVIQTYSYRCSSTFELEPLKAMLDDKYVYGLLVIDRREA
YWGFLRGTHIEPINGTNSTVPGKQRKGGQSSVRFERLRLIAIHDFYSKVGERASAAFLAEPNFFEKFKGVLIGGPMPTKE
EFASGDFLHHEVKKRIIGLFDASYTNEFGLRELVDNAQDALRGVGIMDEKAEMSRFFKELIKENGAAAYGEDSVRLNLEA
GAVDTLLLSEKLRKARLTIKCGNCDYEEIKTMQIEAGKKFKDLPFGRCPKCQSSLFLEKEADIIDELTELADASNTRVEI
ISEDFEEGAMLYSAFGGIAAVLRYPTGM

Sequences:

>Translated_428_residues
MAEEHSQHEIEKDEARKRYEFKKSLERLEEKEGSGTELISLYIPPDKQIYDVTAQLRDEYGQCANIKSKQTRSNVQSALS
SILARLKYFKTPPENGMAIFCGAINVGGDKTDLESIIVEPPEVIQTYSYRCSSTFELEPLKAMLDDKYVYGLLVIDRREA
YWGFLRGTHIEPINGTNSTVPGKQRKGGQSSVRFERLRLIAIHDFYSKVGERASAAFLAEPNFFEKFKGVLIGGPMPTKE
EFASGDFLHHEVKKRIIGLFDASYTNEFGLRELVDNAQDALRGVGIMDEKAEMSRFFKELIKENGAAAYGEDSVRLNLEA
GAVDTLLLSEKLRKARLTIKCGNCDYEEIKTMQIEAGKKFKDLPFGRCPKCQSSLFLEKEADIIDELTELADASNTRVEI
ISEDFEEGAMLYSAFGGIAAVLRYPTGM
>Mature_427_residues
AEEHSQHEIEKDEARKRYEFKKSLERLEEKEGSGTELISLYIPPDKQIYDVTAQLRDEYGQCANIKSKQTRSNVQSALSS
ILARLKYFKTPPENGMAIFCGAINVGGDKTDLESIIVEPPEVIQTYSYRCSSTFELEPLKAMLDDKYVYGLLVIDRREAY
WGFLRGTHIEPINGTNSTVPGKQRKGGQSSVRFERLRLIAIHDFYSKVGERASAAFLAEPNFFEKFKGVLIGGPMPTKEE
FASGDFLHHEVKKRIIGLFDASYTNEFGLRELVDNAQDALRGVGIMDEKAEMSRFFKELIKENGAAAYGEDSVRLNLEAG
AVDTLLLSEKLRKARLTIKCGNCDYEEIKTMQIEAGKKFKDLPFGRCPKCQSSLFLEKEADIIDELTELADASNTRVEII
SEDFEEGAMLYSAFGGIAAVLRYPTGM

Specific function: Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA [H]

COG id: COG1503

COG function: function code J; Peptide chain release factor 1 (eRF1)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic release factor 1 family [H]

Homologues:

Organism=Homo sapiens, GI4759034, Length=415, Percent_Identity=31.0843373493976, Blast_Score=208, Evalue=9e-54,
Organism=Caenorhabditis elegans, GI72000368, Length=414, Percent_Identity=28.9855072463768, Blast_Score=187, Evalue=7e-48,
Organism=Saccharomyces cerevisiae, GI6319619, Length=419, Percent_Identity=31.980906921241, Blast_Score=194, Evalue=2e-50,
Organism=Drosophila melanogaster, GI28574632, Length=433, Percent_Identity=32.5635103926097, Blast_Score=208, Evalue=5e-54,
Organism=Drosophila melanogaster, GI21356433, Length=433, Percent_Identity=32.5635103926097, Blast_Score=208, Evalue=5e-54,
Organism=Drosophila melanogaster, GI24667392, Length=433, Percent_Identity=32.5635103926097, Blast_Score=208, Evalue=5e-54,
Organism=Drosophila melanogaster, GI24667388, Length=433, Percent_Identity=32.5635103926097, Blast_Score=208, Evalue=5e-54,
Organism=Drosophila melanogaster, GI24667380, Length=433, Percent_Identity=32.5635103926097, Blast_Score=208, Evalue=5e-54,
Organism=Drosophila melanogaster, GI24667384, Length=433, Percent_Identity=32.5635103926097, Blast_Score=208, Evalue=5e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005140
- InterPro:   IPR005141
- InterPro:   IPR005142
- InterPro:   IPR020918
- InterPro:   IPR004403 [H]

Pfam domain/function: PF03463 eRF1_1; PF03464 eRF1_2; PF03465 eRF1_3 [H]

EC number: NA

Molecular weight: Translated: 48143; Mature: 48012

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEEHSQHEIEKDEARKRYEFKKSLERLEEKEGSGTELISLYIPPDKQIYDVTAQLRDEY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHH
GQCANIKSKQTRSNVQSALSSILARLKYFKTPPENGMAIFCGAINVGGDKTDLESIIVEP
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCHHHHHHHHCCC
PEVIQTYSYRCSSTFELEPLKAMLDDKYVYGLLVIDRREAYWGFLRGTHIEPINGTNSTV
HHHHHHHHHCCCCCCCCCHHHHHHCCCEEEEEEEEECCHHHHHHHCCCCCCCCCCCCCCC
PGKQRKGGQSSVRFERLRLIAIHDFYSKVGERASAAFLAEPNFFEKFKGVLIGGPMPTKE
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHCEEEECCCCHHHHHCCEEECCCCCCHH
EFASGDFLHHEVKKRIIGLFDASYTNEFGLRELVDNAQDALRGVGIMDEKAEMSRFFKEL
HHCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
IKENGAAAYGEDSVRLNLEAGAVDTLLLSEKLRKARLTIKCGNCDYEEIKTMQIEAGKKF
HHCCCCEECCCCCEEEEEECCCHHHHHHHHHHHHCEEEEEECCCCHHHHHEEEECCCCCC
KDLPFGRCPKCQSSLFLEKEADIIDELTELADASNTRVEIISEDFEEGAMLYSAFGGIAA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHH
VLRYPTGM
HHHCCCCC
>Mature Secondary Structure 
AEEHSQHEIEKDEARKRYEFKKSLERLEEKEGSGTELISLYIPPDKQIYDVTAQLRDEY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHH
GQCANIKSKQTRSNVQSALSSILARLKYFKTPPENGMAIFCGAINVGGDKTDLESIIVEP
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCHHHHHHHHCCC
PEVIQTYSYRCSSTFELEPLKAMLDDKYVYGLLVIDRREAYWGFLRGTHIEPINGTNSTV
HHHHHHHHHCCCCCCCCCHHHHHHCCCEEEEEEEEECCHHHHHHHCCCCCCCCCCCCCCC
PGKQRKGGQSSVRFERLRLIAIHDFYSKVGERASAAFLAEPNFFEKFKGVLIGGPMPTKE
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHCEEEECCCCHHHHHCCEEECCCCCCHH
EFASGDFLHHEVKKRIIGLFDASYTNEFGLRELVDNAQDALRGVGIMDEKAEMSRFFKEL
HHCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
IKENGAAAYGEDSVRLNLEAGAVDTLLLSEKLRKARLTIKCGNCDYEEIKTMQIEAGKKF
HHCCCCEECCCCCEEEEEECCCHHHHHHHHHHHHCEEEEEECCCCHHHHHEEEECCCCCC
KDLPFGRCPKCQSSLFLEKEADIIDELTELADASNTRVEIISEDFEEGAMLYSAFGGIAA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHH
VLRYPTGM
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA