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The map label for this gene is proS

Identifier: 124486203

GI number: 124486203

Start: 1411673

End: 1413106

Strand: Reverse

Name: proS

Synonym: Mlab_1386

Alternate gene names: 124486203

Gene position: 1413106-1411673 (Counterclockwise)

Preceding gene: 124486207

Following gene: 124486202

Centisome position: 78.29

GC content: 53.97

Gene sequence:

>1434_bases
ATGGCAGAAGAAACCGGGGCACTCCCCTCCCGTACCGATTTTAGCGCATGGTACAACGAAGTTATCCGCCGTGCGGATAT
TATGGACGTTAGGTATCCCGTCAAAGGACTCTACGTCTGGTATCCGTTCGGATTTGCACTCCGCAATCACACATACACCC
TTCTTAGATCGCTTCTAAATAGAGATCACGAGGAAACCCTCTTCCCCCTTCTCATCCCGGAAACCGAGTTCATGAAGGAA
GCCGAGCACATCAAAGGATTCGAGGACGAGGTCTACTGGGTCACCCACGGCGGTCTCTCACCGCTTGACGTTAAGCTCGC
TCTTCGTCCGACCAGTGAGACGGCAATCTATCCGATGTACGCTCTGTGGGTACGTTCCCACGCCGATCTTCCATTAAAGC
TCTATCAGGTCGTCAACACCTTTAGATATGAAACAAAACACACCCGCCCCCTGATCCGGCTTAGGGAAATCACCTCTTTC
ATGGAATCCCACACGGTCCACACCGACTGGGACGATGCAAATAAACAGGTCGAGTATGAGCTTGGTCTTGCCACCGAATT
CTATCGTGATCTCGGTGTGCCGATCATCATCTCCCGCCGCCCGGACTGGGACAAGTTCCCCGGAGCTGACTTTACGATGG
CAATCGATGCAGTCATGCCCGACGGCCGGACCCTGCAGATCGGAACCGTTCACCATCTCGGCGACCACTTCTCCACGACC
TATAATATCACCTATGAGGATGTGAACGGCGAACAGAAACTTGCTTCGCAGACCTGCTTCGGCATTTCCGAGAGATGCAT
TGCCGCGATCATCGCGGTTCACGGTGATGACAAAGGCCTGGTCCTTCCGGCAACCGTTGCCCCGACCCAGGTCGTTATCA
TCCCGATCATCGTTGGAAAACGCGGTGACGAGATCATGGCTGCGGTGGAAAAACTCGAGAGCGAACTGAAAGCCGCCGGG
CTCCGTGTGAAAACGGATGCACGCGATATGCGGCCGGGCGCCAAGTATTATCACTGGGAACTCCACGGCGTGCCGCTTCG
CGTAGAACTCGGCCCGCGCGATCTTGACAATAATCAGCTCGTCTGTGCAAACAGGCTTGGCGTCAAGACCACGATCCCAA
GAGAAAATGCCGCCGATTCCGTGAAGCGTCTGCTTGATGAAGCACACGATCAGATCCTCGAAAAAGCCGAAGACCATCTG
AGCAGTCATCTGAAGACCGTTAAGACCGTAGATGAGTGTAATCAGAGTTTAGAGGAAAACATCGTCATTGTTCACTGGTG
CGGAGAGAAGGCCTGCGCCGATAAGCTTGAGGAGCTCACCAACTCATCCCTTCTTGGAACGGGTGTCCGCAGCAAATATG
TCGTCGATGATGAAGGACCGTGCATCGTCTGCGGCAAACCGGGAAAAACCGCTCTCGTCGGCAGATCATACTAA

Upstream 100 bases:

>100_bases
CAATATATCTTGTGGGATATATCTCCATGATAAGCGTCTCCTGACGGAACGTGGGATTAATACTATCAAGGGCCCAATAA
TAACTACTTCAGGTGAATAT

Downstream 100 bases:

>100_bases
AATAATTTTACACACAATCAAAAAAATTCATATAAAAACAGAGGATATCATGTCAAACAATGATCAATATTCAAAAACAC
AAAATGATAAAGCCGCATCC

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MAEETGALPSRTDFSAWYNEVIRRADIMDVRYPVKGLYVWYPFGFALRNHTYTLLRSLLNRDHEETLFPLLIPETEFMKE
AEHIKGFEDEVYWVTHGGLSPLDVKLALRPTSETAIYPMYALWVRSHADLPLKLYQVVNTFRYETKHTRPLIRLREITSF
MESHTVHTDWDDANKQVEYELGLATEFYRDLGVPIIISRRPDWDKFPGADFTMAIDAVMPDGRTLQIGTVHHLGDHFSTT
YNITYEDVNGEQKLASQTCFGISERCIAAIIAVHGDDKGLVLPATVAPTQVVIIPIIVGKRGDEIMAAVEKLESELKAAG
LRVKTDARDMRPGAKYYHWELHGVPLRVELGPRDLDNNQLVCANRLGVKTTIPRENAADSVKRLLDEAHDQILEKAEDHL
SSHLKTVKTVDECNQSLEENIVIVHWCGEKACADKLEELTNSSLLGTGVRSKYVVDDEGPCIVCGKPGKTALVGRSY

Sequences:

>Translated_477_residues
MAEETGALPSRTDFSAWYNEVIRRADIMDVRYPVKGLYVWYPFGFALRNHTYTLLRSLLNRDHEETLFPLLIPETEFMKE
AEHIKGFEDEVYWVTHGGLSPLDVKLALRPTSETAIYPMYALWVRSHADLPLKLYQVVNTFRYETKHTRPLIRLREITSF
MESHTVHTDWDDANKQVEYELGLATEFYRDLGVPIIISRRPDWDKFPGADFTMAIDAVMPDGRTLQIGTVHHLGDHFSTT
YNITYEDVNGEQKLASQTCFGISERCIAAIIAVHGDDKGLVLPATVAPTQVVIIPIIVGKRGDEIMAAVEKLESELKAAG
LRVKTDARDMRPGAKYYHWELHGVPLRVELGPRDLDNNQLVCANRLGVKTTIPRENAADSVKRLLDEAHDQILEKAEDHL
SSHLKTVKTVDECNQSLEENIVIVHWCGEKACADKLEELTNSSLLGTGVRSKYVVDDEGPCIVCGKPGKTALVGRSY
>Mature_476_residues
AEETGALPSRTDFSAWYNEVIRRADIMDVRYPVKGLYVWYPFGFALRNHTYTLLRSLLNRDHEETLFPLLIPETEFMKEA
EHIKGFEDEVYWVTHGGLSPLDVKLALRPTSETAIYPMYALWVRSHADLPLKLYQVVNTFRYETKHTRPLIRLREITSFM
ESHTVHTDWDDANKQVEYELGLATEFYRDLGVPIIISRRPDWDKFPGADFTMAIDAVMPDGRTLQIGTVHHLGDHFSTTY
NITYEDVNGEQKLASQTCFGISERCIAAIIAVHGDDKGLVLPATVAPTQVVIIPIIVGKRGDEIMAAVEKLESELKAAGL
RVKTDARDMRPGAKYYHWELHGVPLRVELGPRDLDNNQLVCANRLGVKTTIPRENAADSVKRLLDEAHDQILEKAEDHLS
SHLKTVKTVDECNQSLEENIVIVHWCGEKACADKLEELTNSSLLGTGVRSKYVVDDEGPCIVCGKPGKTALVGRSY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily

Homologues:

Organism=Homo sapiens, GI62241042, Length=492, Percent_Identity=33.9430894308943, Blast_Score=279, Evalue=5e-75,
Organism=Escherichia coli, GI1786392, Length=149, Percent_Identity=34.2281879194631, Blast_Score=90, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71991342, Length=437, Percent_Identity=37.9862700228833, Blast_Score=296, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI71991346, Length=354, Percent_Identity=40.3954802259887, Blast_Score=263, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6321808, Length=440, Percent_Identity=33.6363636363636, Blast_Score=266, Evalue=6e-72,
Organism=Drosophila melanogaster, GI24649468, Length=489, Percent_Identity=37.0143149284254, Blast_Score=290, Evalue=1e-78,
Organism=Drosophila melanogaster, GI24649466, Length=489, Percent_Identity=37.0143149284254, Blast_Score=290, Evalue=1e-78,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_METLZ (A2ST96)

Other databases:

- EMBL:   CP000559
- RefSeq:   YP_001030819.1
- ProteinModelPortal:   A2ST96
- SMR:   A2ST96
- STRING:   A2ST96
- GeneID:   4795368
- GenomeReviews:   CP000559_GR
- KEGG:   mla:Mlab_1386
- eggNOG:   arNOG04466
- HOGENOM:   HBG334108
- OMA:   PGWKFNE
- PhylomeDB:   A2ST96
- ProtClustDB:   PRK08661
- BioCyc:   MLAB410358:MLAB_1386-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01571
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.30.110.30
- PANTHER:   PTHR11451:SF6
- PRINTS:   PR01046
- SMART:   SM00946
- TIGRFAMs:   TIGR00408

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd; SSF64586 Pro-tRNA_synth_II_C

EC number: =6.1.1.15

Molecular weight: Translated: 53874; Mature: 53743

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEETGALPSRTDFSAWYNEVIRRADIMDVRYPVKGLYVWYPFGFALRNHTYTLLRSLLN
CCCCCCCCCCCCCHHHHHHHHHHHHHHHEEECCCCEEEEEEECCHHHHHHHHHHHHHHHC
RDHEETLFPLLIPETEFMKEAEHIKGFEDEVYWVTHGGLSPLDVKLALRPTSETAIYPMY
CCCCCCEEEEECCCHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEEEECCCCCCHHHHH
ALWVRSHADLPLKLYQVVNTFRYETKHTRPLIRLREITSFMESHTVHTDWDDANKQVEYE
HHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEE
LGLATEFYRDLGVPIIISRRPDWDKFPGADFTMAIDAVMPDGRTLQIGTVHHLGDHFSTT
ECHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEEEEECCCCCEEEEECHHHCCCCCCEE
YNITYEDVNGEQKLASQTCFGISERCIAAIIAVHGDDKGLVLPATVAPTQVVIIPIIVGK
EEEEEECCCCHHHHHHHHHCCHHHHHHEEEEEEECCCCCEEEEECCCCCEEEEEEEEECC
RGDEIMAAVEKLESELKAAGLRVKTDARDMRPGAKYYHWELHGVPLRVELGPRDLDNNQL
CCHHHHHHHHHHHHHHHHCCCEEECCHHHCCCCCCEEEEEEECEEEEEEECCCCCCCCCE
VCANRLGVKTTIPRENAADSVKRLLDEAHDQILEKAEDHLSSHLKTVKTVDECNQSLEEN
EEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
IVIVHWCGEKACADKLEELTNSSLLGTGVRSKYVVDDEGPCIVCGKPGKTALVGRSY
EEEEEECCCHHHHHHHHHHCCCCEEECCCCCEEEECCCCCEEEECCCCCEEECCCCC
>Mature Secondary Structure 
AEETGALPSRTDFSAWYNEVIRRADIMDVRYPVKGLYVWYPFGFALRNHTYTLLRSLLN
CCCCCCCCCCCCHHHHHHHHHHHHHHHEEECCCCEEEEEEECCHHHHHHHHHHHHHHHC
RDHEETLFPLLIPETEFMKEAEHIKGFEDEVYWVTHGGLSPLDVKLALRPTSETAIYPMY
CCCCCCEEEEECCCHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEEEECCCCCCHHHHH
ALWVRSHADLPLKLYQVVNTFRYETKHTRPLIRLREITSFMESHTVHTDWDDANKQVEYE
HHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEE
LGLATEFYRDLGVPIIISRRPDWDKFPGADFTMAIDAVMPDGRTLQIGTVHHLGDHFSTT
ECHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEEEEECCCCCEEEEECHHHCCCCCCEE
YNITYEDVNGEQKLASQTCFGISERCIAAIIAVHGDDKGLVLPATVAPTQVVIIPIIVGK
EEEEEECCCCHHHHHHHHHCCHHHHHHEEEEEEECCCCCEEEEECCCCCEEEEEEEEECC
RGDEIMAAVEKLESELKAAGLRVKTDARDMRPGAKYYHWELHGVPLRVELGPRDLDNNQL
CCHHHHHHHHHHHHHHHHCCCEEECCHHHCCCCCCEEEEEEECEEEEEEECCCCCCCCCE
VCANRLGVKTTIPRENAADSVKRLLDEAHDQILEKAEDHLSSHLKTVKTVDECNQSLEEN
EEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
IVIVHWCGEKACADKLEELTNSSLLGTGVRSKYVVDDEGPCIVCGKPGKTALVGRSY
EEEEEECCCHHHHHHHHHHCCCCEEECCCCCEEEECCCCCEEEECCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA