Click here to switch to the map view.

The map label for this gene is lcfA [H]

Identifier: 124486052

GI number: 124486052

Start: 1261498

End: 1263204

Strand: Reverse

Name: lcfA [H]

Synonym: Mlab_1232

Alternate gene names: 124486052

Gene position: 1263204-1261498 (Counterclockwise)

Preceding gene: 124486053

Following gene: 124486051

Centisome position: 69.99

GC content: 52.49

Gene sequence:

>1707_bases
TTGACATTACATTACTCTGAAGAAAACAAACGTTCGCTGTACGAAGCACTTCTATATGGTTTCAATAGGGCCGGCAATAC
ATCACCCGCGGCCGCATATTACGGAAGGCACATCCTGTTCAAACAGTTCATGGACGAGGTTCATGCGATCGCGGCAGCCC
TGGTGAATCATGGTGTGAAAAAAGGCGATTATGTCACTATTTTCCTGCCGAACATCCCTCAGGGGGTTCTGGCTCTCTAT
GCCGTCAACCGGATCGGCGCAATATGCAATATGGTCCATCCCTTATCCCCTCAGGAAGATATCGATTATGCCCTTAAACT
CACAGACAGCCGGATCGCTCTCGCCTGCGAGGTCAATGAAGGTCTTCTCTCCGGGAAAAATATCGAGGTCATCAGATGTC
GGACCTCCGGCTACTTCCCTTCAACCCCCAAAGGATTCGTGCTGGACAAAGGATTCCGGTTTGTGATGCGCAAATATCCA
AAGGCAGAGAACGTTGCAAAAATCACGCTCTGGACCGATCTTCTTGCCGAAGGAAAGAAACTGCTCAAAGGAGGATTCCA
GCTGCCGTCTGACGACGTCCAGCCCGACGATACCGCTGTGATCATGTACACCGGCGGAACAACGGGTGACTCGAAAGGCG
TCATGCTCTCCAATTACGCGGTAAACTCGATGTCCATCCAGCTCTTGATCGACATCGGCGAGGGTAAAACCGATGTGGGC
GACGGGTTCCTTGCGATCCTTCCGATCTTCCATGCATTCGGTCTGGCAGTTTCCGTTCATGCGCCGCTTATCTCCGGTAT
GAAGATCGTTCTGATCCCAAGATTCGATCCGAAAGGATGCGCCCAGCAGATCTTCTCCGAAGACATCCTGTTCCTCCCGG
GAGTCCCCGCTCTGTTCGAACGCATGTATCCCTACTTCGAAAATAAAGATCTCTCCAGAATGAAGCTCATGGTCAGCGGA
GGAGACCGCGTCTCCGAAGAGCTCGCCAATAAATACAATGTTCTTTTACGAAAATCCAAGGCCGATATCCTCTTTAGGAC
CGGGTACGGGCTGACGGAGGCGTGCGGTGCCTGCTGTCTCGTCGCGAACCAGTATGAAAAACTCCCGACCGGCTGTGTCG
GGTCTCCGGTTACCGGCACAAAAGTCTGCGTTGTAAAACCCGGCACAACGGAACTCGTACCGGACGGTGTGGAAGGAGAA
CTCTGTTTCCTCGGTCCTTCGGTGATGAAAGGATACTACAAGAACGAAGAAGCCACAAAGGATGTTCTCCGGCTCCATGA
CGATGGAAACGTCTGGCTCCACACCGGCGATCTTGTCGTTATCAGGAAGGACGGGAACGTCTGTTTCCGTTCCCGGCACA
AACGCCTCGTGAAGGTGAACGGCTACAATGTGTATCCAACCCTTATCGAGGAGGCGATGCAGAACCATCGCGATATCAAA
CTGGTCTGCGCCGTTGCGACCCCCTGGAAACTTGACCGGAAGATCAAACTCTTCGTTGTTCCGGAAAAGCCGCTCGGCTC
CTTTGATGCGGCTGAGGAAGAGAAGATCCTGATCGATTACGCCAAAGACCAGATGAACCGCTGGAGCGTTCCGGCCAGGG
TGGAGTTCGTGGCCGATCTTCCCATGACCAAGTTCAACAAGATAGATTACCGTCTGCTGGAAAAGCAGGAACTTTCACGG
GCAAACGATAAAAAGCAGGCTGAGTAA

Upstream 100 bases:

>100_bases
TTAAGTGATAAAATAGACAAAAATATTTCCGTTTCTATTATATGTTCCCCCGTAAATAATACTATGAGATTTTTTTACCG
CTTGATCGGAGGATACATAT

Downstream 100 bases:

>100_bases
CGTATGTCACGTCCTGTGTTTAGAGACGACGCGAAGACCGAACTGGAATACACCGACGAGACGAAACAGTCTCTCTATTC
CATGTTCAACTACGGCGTGC

Product: hypothetical protein

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 568; Mature: 567

Protein sequence:

>568_residues
MTLHYSEENKRSLYEALLYGFNRAGNTSPAAAYYGRHILFKQFMDEVHAIAAALVNHGVKKGDYVTIFLPNIPQGVLALY
AVNRIGAICNMVHPLSPQEDIDYALKLTDSRIALACEVNEGLLSGKNIEVIRCRTSGYFPSTPKGFVLDKGFRFVMRKYP
KAENVAKITLWTDLLAEGKKLLKGGFQLPSDDVQPDDTAVIMYTGGTTGDSKGVMLSNYAVNSMSIQLLIDIGEGKTDVG
DGFLAILPIFHAFGLAVSVHAPLISGMKIVLIPRFDPKGCAQQIFSEDILFLPGVPALFERMYPYFENKDLSRMKLMVSG
GDRVSEELANKYNVLLRKSKADILFRTGYGLTEACGACCLVANQYEKLPTGCVGSPVTGTKVCVVKPGTTELVPDGVEGE
LCFLGPSVMKGYYKNEEATKDVLRLHDDGNVWLHTGDLVVIRKDGNVCFRSRHKRLVKVNGYNVYPTLIEEAMQNHRDIK
LVCAVATPWKLDRKIKLFVVPEKPLGSFDAAEEEKILIDYAKDQMNRWSVPARVEFVADLPMTKFNKIDYRLLEKQELSR
ANDKKQAE

Sequences:

>Translated_568_residues
MTLHYSEENKRSLYEALLYGFNRAGNTSPAAAYYGRHILFKQFMDEVHAIAAALVNHGVKKGDYVTIFLPNIPQGVLALY
AVNRIGAICNMVHPLSPQEDIDYALKLTDSRIALACEVNEGLLSGKNIEVIRCRTSGYFPSTPKGFVLDKGFRFVMRKYP
KAENVAKITLWTDLLAEGKKLLKGGFQLPSDDVQPDDTAVIMYTGGTTGDSKGVMLSNYAVNSMSIQLLIDIGEGKTDVG
DGFLAILPIFHAFGLAVSVHAPLISGMKIVLIPRFDPKGCAQQIFSEDILFLPGVPALFERMYPYFENKDLSRMKLMVSG
GDRVSEELANKYNVLLRKSKADILFRTGYGLTEACGACCLVANQYEKLPTGCVGSPVTGTKVCVVKPGTTELVPDGVEGE
LCFLGPSVMKGYYKNEEATKDVLRLHDDGNVWLHTGDLVVIRKDGNVCFRSRHKRLVKVNGYNVYPTLIEEAMQNHRDIK
LVCAVATPWKLDRKIKLFVVPEKPLGSFDAAEEEKILIDYAKDQMNRWSVPARVEFVADLPMTKFNKIDYRLLEKQELSR
ANDKKQAE
>Mature_567_residues
TLHYSEENKRSLYEALLYGFNRAGNTSPAAAYYGRHILFKQFMDEVHAIAAALVNHGVKKGDYVTIFLPNIPQGVLALYA
VNRIGAICNMVHPLSPQEDIDYALKLTDSRIALACEVNEGLLSGKNIEVIRCRTSGYFPSTPKGFVLDKGFRFVMRKYPK
AENVAKITLWTDLLAEGKKLLKGGFQLPSDDVQPDDTAVIMYTGGTTGDSKGVMLSNYAVNSMSIQLLIDIGEGKTDVGD
GFLAILPIFHAFGLAVSVHAPLISGMKIVLIPRFDPKGCAQQIFSEDILFLPGVPALFERMYPYFENKDLSRMKLMVSGG
DRVSEELANKYNVLLRKSKADILFRTGYGLTEACGACCLVANQYEKLPTGCVGSPVTGTKVCVVKPGTTELVPDGVEGEL
CFLGPSVMKGYYKNEEATKDVLRLHDDGNVWLHTGDLVVIRKDGNVCFRSRHKRLVKVNGYNVYPTLIEEAMQNHRDIKL
VCAVATPWKLDRKIKLFVVPEKPLGSFDAAEEEKILIDYAKDQMNRWSVPARVEFVADLPMTKFNKIDYRLLEKQELSRA
NDKKQAE

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=421, Percent_Identity=26.603325415677, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI187761343, Length=421, Percent_Identity=26.603325415677, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI156151445, Length=563, Percent_Identity=23.2682060390764, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI58082049, Length=560, Percent_Identity=21.9642857142857, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI157311624, Length=564, Percent_Identity=21.6312056737589, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI157311622, Length=564, Percent_Identity=21.6312056737589, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI42544132, Length=516, Percent_Identity=22.4806201550388, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI13375727, Length=546, Percent_Identity=23.6263736263736, Blast_Score=83, Evalue=8e-16,
Organism=Homo sapiens, GI12669909, Length=371, Percent_Identity=25.8760107816712, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI4758332, Length=371, Percent_Identity=25.8760107816712, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI122937307, Length=527, Percent_Identity=23.5294117647059, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI115511026, Length=537, Percent_Identity=21.9739292364991, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI38505220, Length=530, Percent_Identity=21.8867924528302, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1788107, Length=550, Percent_Identity=28.9090909090909, Blast_Score=188, Evalue=8e-49,
Organism=Escherichia coli, GI145693145, Length=368, Percent_Identity=24.4565217391304, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789201, Length=375, Percent_Identity=25.8666666666667, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1786529, Length=544, Percent_Identity=24.6323529411765, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI1786810, Length=538, Percent_Identity=19.8884758364312, Blast_Score=70, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17559526, Length=411, Percent_Identity=28.9537712895377, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17558820, Length=524, Percent_Identity=25, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71994694, Length=538, Percent_Identity=24.3494423791822, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI71994703, Length=538, Percent_Identity=24.3494423791822, Blast_Score=122, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI71994690, Length=538, Percent_Identity=24.3494423791822, Blast_Score=122, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17531443, Length=571, Percent_Identity=24.5183887915937, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI32563687, Length=522, Percent_Identity=24.3295019157088, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17538037, Length=560, Percent_Identity=24.8214285714286, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17557194, Length=545, Percent_Identity=24.5871559633028, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17560308, Length=455, Percent_Identity=22.8571428571429, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17560140, Length=524, Percent_Identity=23.2824427480916, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71985884, Length=304, Percent_Identity=26.9736842105263, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI25147511, Length=438, Percent_Identity=24.6575342465753, Blast_Score=82, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17553312, Length=436, Percent_Identity=23.8532110091743, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17510401, Length=159, Percent_Identity=32.0754716981132, Blast_Score=65, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6323182, Length=560, Percent_Identity=23.5714285714286, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6319264, Length=546, Percent_Identity=25.6410256410256, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6319699, Length=373, Percent_Identity=22.7882037533512, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI18859661, Length=534, Percent_Identity=24.7191011235955, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24581924, Length=537, Percent_Identity=24.7672253258845, Blast_Score=125, Evalue=9e-29,
Organism=Drosophila melanogaster, GI21355181, Length=561, Percent_Identity=25.4901960784314, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI21356441, Length=383, Percent_Identity=26.6318537859008, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI281365686, Length=341, Percent_Identity=26.6862170087977, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648253, Length=381, Percent_Identity=24.4094488188976, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24648255, Length=381, Percent_Identity=24.6719160104987, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21358303, Length=292, Percent_Identity=28.4246575342466, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19922652, Length=541, Percent_Identity=21.6266173752311, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24656500, Length=537, Percent_Identity=23.2774674115456, Blast_Score=75, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 63162; Mature: 63031

Theoretical pI: Translated: 8.00; Mature: 8.00

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLHYSEENKRSLYEALLYGFNRAGNTSPAAAYYGRHILFKQFMDEVHAIAAALVNHGVK
CEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KGDYVTIFLPNIPQGVLALYAVNRIGAICNMVHPLSPQEDIDYALKLTDSRIALACEVNE
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCEEEEEEECCC
GLLSGKNIEVIRCRTSGYFPSTPKGFVLDKGFRFVMRKYPKAENVAKITLWTDLLAEGKK
CCCCCCCEEEEEEECCCCCCCCCCCCEECCHHHHHHHHCCCCCCEEEEEEHHHHHHHHHH
LLKGGFQLPSDDVQPDDTAVIMYTGGTTGDSKGVMLSNYAVNSMSIQLLIDIGEGKTDVG
HHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEEEECCEEEEEEEECCCCCCCCC
DGFLAILPIFHAFGLAVSVHAPLISGMKIVLIPRFDPKGCAQQIFSEDILFLPGVPALFE
CCHHHHHHHHHHHHHHEEECCHHHCCCEEEEEECCCCCHHHHHHHCCCCEECCCCHHHHH
RMYPYFENKDLSRMKLMVSGGDRVSEELANKYNVLLRKSKADILFRTGYGLTEACGACCL
HHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHEEEEECCCCEEEECCCCHHHHHHHHHH
VANQYEKLPTGCVGSPVTGTKVCVVKPGTTELVPDGVEGELCFLGPSVMKGYYKNEEATK
HHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEECHHHHHHHHCCCHHHH
DVLRLHDDGNVWLHTGDLVVIRKDGNVCFRSRHKRLVKVNGYNVYPTLIEEAMQNHRDIK
HHHEEECCCCEEEEECCEEEEECCCCCHHHCCCCEEEEECCEEECHHHHHHHHHCCCCEE
LVCAVATPWKLDRKIKLFVVPEKPLGSFDAAEEEKILIDYAKDQMNRWSVPARVEFVADL
EEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEHHHHHHHCCCCEEEEEEECC
PMTKFNKIDYRLLEKQELSRANDKKQAE
CCHHHCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TLHYSEENKRSLYEALLYGFNRAGNTSPAAAYYGRHILFKQFMDEVHAIAAALVNHGVK
EEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KGDYVTIFLPNIPQGVLALYAVNRIGAICNMVHPLSPQEDIDYALKLTDSRIALACEVNE
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCEEEEEEECCC
GLLSGKNIEVIRCRTSGYFPSTPKGFVLDKGFRFVMRKYPKAENVAKITLWTDLLAEGKK
CCCCCCCEEEEEEECCCCCCCCCCCCEECCHHHHHHHHCCCCCCEEEEEEHHHHHHHHHH
LLKGGFQLPSDDVQPDDTAVIMYTGGTTGDSKGVMLSNYAVNSMSIQLLIDIGEGKTDVG
HHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEEEECCEEEEEEEECCCCCCCCC
DGFLAILPIFHAFGLAVSVHAPLISGMKIVLIPRFDPKGCAQQIFSEDILFLPGVPALFE
CCHHHHHHHHHHHHHHEEECCHHHCCCEEEEEECCCCCHHHHHHHCCCCEECCCCHHHHH
RMYPYFENKDLSRMKLMVSGGDRVSEELANKYNVLLRKSKADILFRTGYGLTEACGACCL
HHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHEEEEECCCCEEEECCCCHHHHHHHHHH
VANQYEKLPTGCVGSPVTGTKVCVVKPGTTELVPDGVEGELCFLGPSVMKGYYKNEEATK
HHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEECHHHHHHHHCCCHHHH
DVLRLHDDGNVWLHTGDLVVIRKDGNVCFRSRHKRLVKVNGYNVYPTLIEEAMQNHRDIK
HHHEEECCCCEEEEECCEEEEECCCCCHHHCCCCEEEEECCEEECHHHHHHHHHCCCCEE
LVCAVATPWKLDRKIKLFVVPEKPLGSFDAAEEEKILIDYAKDQMNRWSVPARVEFVADL
EEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEHHHHHHHCCCCEEEEEEECC
PMTKFNKIDYRLLEKQELSRANDKKQAE
CCHHHCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]